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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
3164R-3 |
| Symbol |
CG3164 |
Full Name |
CG3164 |
| CG No |
CG3164 |
Old CG No |
CG3164 |
| Synonyms |
CK02656, BEST:CK02656, CG3164 |
| Accession No (Link to NCBI) |
NM_164366.1 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgAGTACCCGCCCAGCCAAAGAC |
| Primer Seq. 3' |
aatctagaggtaccAGAGCCGCTTTTTCTGACCGC |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 AGTACCCGCC CAGCCAAAGA CGCTCCAGCA CCTCCCCAAG CGACCAGCGG TGGACCTGGC
0061 CTTTCACNAA CCTGACGTAC CGCGTCAAGG AGGGGAACCG GAGCAATGCC AAGACCATTC
0121 TGAAGGGCGT CAGTGGACGc CTCCGGTCTG GAGNAACTCA CCGCCATAAT GGGACCATCG
0181 GGTGCTGGCA AGAGTACGCT GCTCAACATT TTGTCTGGCT ACAAAACTTC GAGCATCGAA
0241 GGCAGCGTTA CGATGAACGG TGCCGAGCGC AACCTAAGCG CATTTCGCAA GCTTTCTGCC
0301 TACATCATGC AGGACAATCA GCTTCACGGA AACCTTACCG TCCAGGAAGC AATGACTGTG
0361 GCAACGAATC TGAAGCTCAG CAAGAAGTTT TCGAAGCCCG AGAAGCATTC AATGATCGAT
0421 GACATTTTGC TTACTCTCAG CCTGAGCGAG CACCGATATA CGATGACTCG AAATTTGTCC
0481 GGCGGTCAGA AAAAgNGGCT NCT |
| in silico PCR Fragment |
0001 AGTACCCGCC CAGCCAAAGA CGCTCCAGCA CCTCCCCAAG CGACCAGCGG TGGACCTGGC
0061 CTTTCAC-AA CCTGACGTAC CGCGTCAAGG AGGGGAACCG GAGCAATGCC AAGACCATTC
0121 TGAAGGGCGT CAGTGGACGC CTCCGGTCTG GAG-AACTCA CCGCCATAAT GGGACCATCG
0181 GGTGCTGGCA AGAGTACGCT GCTCAACATT TTGTCTGGCT ACAAAACTTC GAGCATCGAA
0241 GGCAGCGTTA CGATGAACGG TGCCGAGCGC AACCTAAGCG CATTTCGCAA GCTTTCTGCC
0301 TACATCATGC AGGACAATCA GCTTCACGGA AACCTTACCG TCCAGGAAGC AATGACTGTG
0361 GCAACGAATC TGAAGCTCAG CAAGAAGTTT TCGAAGCCCG AGAAGCATTC AATGATCGAT
0421 GACATTTTGC TTACTCTCAG CCTGAGCGAG CACCGATATA CGATGACTCG AAATTTGTCC
0481 GGCGGTCAGA AAAAGCGGCT -CT |
| Assemble Data |
3164R-3.IR_full 1 AGTACCCGCCCAGCCAAAGACGCTCCAGCACCTCCCCAAGCGACCAGCGGTGGACCTGGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3164R-3.in silico 1 AGTACCCGCCCAGCCAAAGACGCTCCAGCACCTCCCCAAGCGACCAGCGGTGGACCTGGC 60
3164R-3.IR_full 61 CTTTCACNAACCTGACGTACCGCGTCAAGGAGGGGAACCGGAGCAATGCCAAGACCATTC 120
||||||| ||||||||||||||||||||||||||||||||||||||||||||||||||||
3164R-3.in silico 61 CTTTCAC-AACCTGACGTACCGCGTCAAGGAGGGGAACCGGAGCAATGCCAAGACCATTC 120
3164R-3.IR_full 121 TGAAGGGCGTCAGTGGACGCCTCCGGTCTGGAGNAACTCACCGCCATAATGGGACCATCG 180
||||||||||||||||||||||||||||||||| ||||||||||||||||||||||||||
3164R-3.in silico 121 TGAAGGGCGTCAGTGGACGCCTCCGGTCTGGAG-AACTCACCGCCATAATGGGACCATCG 180
3164R-3.IR_full 181 GGTGCTGGCAAGAGTACGCTGCTCAACATTTTGTCTGGCTACAAAACTTCGAGCATCGAA 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3164R-3.in silico 181 GGTGCTGGCAAGAGTACGCTGCTCAACATTTTGTCTGGCTACAAAACTTCGAGCATCGAA 240
3164R-3.IR_full 241 GGCAGCGTTACGATGAACGGTGCCGAGCGCAACCTAAGCGCATTTCGCAAGCTTTCTGCC 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3164R-3.in silico 241 GGCAGCGTTACGATGAACGGTGCCGAGCGCAACCTAAGCGCATTTCGCAAGCTTTCTGCC 300
3164R-3.IR_full 301 TACATCATGCAGGACAATCAGCTTCACGGAAACCTTACCGTCCAGGAAGCAATGACTGTG 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3164R-3.in silico 301 TACATCATGCAGGACAATCAGCTTCACGGAAACCTTACCGTCCAGGAAGCAATGACTGTG 360
3164R-3.IR_full 361 GCAACGAATCTGAAGCTCAGCAAGAAGTTTTCGAAGCCCGAGAAGCATTCAATGATCGAT 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3164R-3.in silico 361 GCAACGAATCTGAAGCTCAGCAAGAAGTTTTCGAAGCCCGAGAAGCATTCAATGATCGAT 420
3164R-3.IR_full 421 GACATTTTGCTTACTCTCAGCCTGAGCGAGCACCGATATACGATGACTCGAAATTTGTCC 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3164R-3.in silico 421 GACATTTTGCTTACTCTCAGCCTGAGCGAGCACCGATATACGATGACTCGAAATTTGTCC 480
3164R-3.IR_full 481 GGCGGTCAGAAAAAGNGGCTNCT 503
||||||||||||||| |||| ||
3164R-3.in silico 481 GGCGGTCAGAAAAAGCGGCT-CT 503
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_205881.2 |
CG3164-RC, transcript variant C (CG3164), mRNA |
| 100 |
482 |
0 |
0 |
0 |
NM_164366.1 |
CG3164-RA, transcript variant A (CG3164), mRNA |
| 100 |
482 |
0 |
0 |
0 |
NM_001038776.1 |
CG3164-RD, transcript variant D (CG3164), mRNA |
| 100 |
482 |
0 |
0 |
0 |
NM_134650.2 |
CG3164-RB, transcript variant B (CG3164), mRNA |
| 0.62 |
3 |
2 |
2 |
2 |
NM_132559.1 |
CG2750-RA (CG2750), mRNA |
| 0 |
0 |
5 |
7 |
5 |
NM_134600.1 |
CG1494-RA (CG1494), mRNA |
| 0 |
0 |
4 |
4 |
5 |
NM_135650.2 |
CG6258-RA (RfC38), mRNA |
| 0 |
0 |
3 |
13 |
13 |
NM_134916.3 |
CG9663-RA (CG9663), mRNA |
| 0 |
0 |
2 |
14 |
14 |
NM_164495.1 |
CG31689-RD, transcript variant D (CG31689), mRNA |
| 0 |
0 |
2 |
14 |
14 |
NM_164494.1 |
CG31689-RB, transcript variant B (CG31689), mRNA |
| 0 |
0 |
2 |
14 |
14 |
NM_134859.2 |
CG31689-RC, transcript variant C (CG31689), mRNA |
| 0 |
0 |
2 |
14 |
14 |
NM_164493.1 |
CG31689-RA, transcript variant A (CG31689), mRNA |
| 0 |
0 |
2 |
5 |
3 |
NM_136966.1 |
CG8771-RA (CG8771), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_058005.3 |
CG9738-RA (Mkk4), mRNA |
| 0 |
0 |
1 |
16 |
23 |
NM_164436.1 |
CG17646-RB, transcript variant B (CG17646), mRNA |
| 0 |
0 |
1 |
16 |
23 |
NM_134774.2 |
CG17646-RA, transcript variant A (CG17646), mRNA |
| 0 |
0 |
1 |
8 |
20 |
NM_078747.2 |
CG2969-RA, transcript variant A (Atet), mRNA |
| 0 |
0 |
1 |
8 |
20 |
NM_164580.1 |
CG2969-RB, transcript variant B (Atet), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_132959.2 |
CG8949-RA (CG8949), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_142354.1 |
CG4090-RA (CG4090), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_136366.1 |
CG8335-RA (CG8335), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_206397.1 |
CG5992-RB, transcript variant B (Adgf-A), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_079406.2 |
CG5992-RA, transcript variant A (Adgf-A), mRNA |
| 0 |
0 |
0 |
9 |
19 |
NM_176005.1 |
CG5853-RA (CG5853), mRNA |
| 0 |
0 |
0 |
5 |
9 |
NM_134281.1 |
CG8651-RC, transcript variant C (trx), mRNA |
| 0 |
0 |
0 |
5 |
9 |
NM_057421.2 |
CG8651-RD, transcript variant D (trx), mRNA |
| 0 |
0 |
0 |
5 |
9 |
NM_057422.2 |
CG8651-RB, transcript variant B (trx), mRNA |
| 0 |
0 |
0 |
5 |
9 |
NM_134282.1 |
CG8651-RA, transcript variant A (trx), mRNA |
| 0 |
0 |
0 |
5 |
9 |
NM_001014621.1 |
CG8651-RE, transcript variant E (trx), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_164561.2 |
CG3920-RB, transcript variant B (l(2)k16918), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
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JNK signal
| N |
 |
| |
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| DB |
 |
| +- |
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| DC |
 |
| +- |
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| DM |
 |
| + |
|
| VM |
 |
| + |
|
| VB |
 |
| |
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Caspase-3 signal
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