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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
31600R-1 |
| Symbol |
CG31600 |
Full Name |
CG31600 |
| CG No |
CG31600 |
Old CG No |
CG31600 |
| Synonyms |
CG31600 |
| Accession No (Link to NCBI) |
NM_165401.1 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340
[
PubMed ID = 25473846
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGGTCAGCCTATACTTAGCCAA |
| Primer Seq. 3' |
aatctagaggtaccCTATTAGTACCGCAAGCCCGT |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 GGTCAGCCTA TACTTAGCCA ATTTGCGTGA TGATTGGCTT GTTCTCGGGC TTATACCGCT
0061 GCAGATACTC GTCCTTCATT GGACTTACTT CGCAATTTCA AAACAACTAG AAGCCATGAA
0121 CAGGAGCTAT AGTACATTCA TGGATAGCTT AAACCTTAAA TGGCTGGCAT ACTCCAATTT
0181 TAACGAGACA GAATGGCCTT CGATTGAAAA TGCGGTTTTA TGCTGTGGAC TGGAGGGTCC
0241 CCGTTCTTAT ATGGATTATC TACAAGGGGT TCCAACCCAC TGCTACCATC CCGACCTTAT
0301 CACCCAAGGT TGCAGTGACT TTGTTAAAAA CATTGTTATG CCGATACAAC AAATAAGTCA
0361 TTTGCAGCTC AGATTGGCTA TTTTTGTGGA ACTGGTAATT TTACTTATTC TTGCTGCAAC
0421 GCTTTTGAAG AAATGTATTT CTTTGATCGG CGAAAGGAAA CATAAAAGAG TTATTACACA
0481 CGGGCTTGCG GTACTAATAG |
| in silico PCR Fragment |
0001 GGTCAGCCTA TACTTAGCCA ATTTGCGTGA TGATTGGCTT GTTCTCGGGC TTATACCGCT
0061 GCAGATACTC GTCCTTCATT GGACTTACTT CACAATTTCA AAACAACTAG AAGCCATGAA
0121 CAGGAGCTAT AGTACATTCA TGGATAGCTT AAACCTTAAA TGGCTGGCAT ACTCCAATTT
0181 TAACGAGACA GATTGGCCTT CGATTGAAAA TGCGGTTTTA TGCTGTGGAC TGGAGGGTCC
0241 CCGTTCTTAT ATGGATTATC TACAAGGGGT TCCAACCCAC TGCTACCATC CCGACCTTAT
0301 CACCCAAGGT TGCAGTGACT TTGTTAAAAA CATTTTTATG CCGATACAAC ATATAAGTCA
0361 TTTGCAGCTC AGATTGGCTA TTTTTGTGGA ACTGGTAATT TTACTTATTC TTGCTGCAAC
0421 GCTTTTGAAG AAATGTATTT CTTTGATCGG CGAAAGGAAA CATAAAAGAG TTATTACACA
0481 CGGGCTTGCG GTACTAATAG |
| Assemble Data |
31600R-1.IR_full 1 GGTCAGCCTATACTTAGCCAATTTGCGTGATGATTGGCTTGTTCTCGGGCTTATACCGCT 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31600R-1.in silico 1 GGTCAGCCTATACTTAGCCAATTTGCGTGATGATTGGCTTGTTCTCGGGCTTATACCGCT 60
31600R-1.IR_full 61 GCAGATACTCGTCCTTCATTGGACTTACTTCGCAATTTCAAAACAACTAGAAGCCATGAA 120
||||||||||||||||||||||||||||||| ||||||||||||||||||||||||||||
31600R-1.in silico 61 GCAGATACTCGTCCTTCATTGGACTTACTTCACAATTTCAAAACAACTAGAAGCCATGAA 120
31600R-1.IR_full 121 CAGGAGCTATAGTACATTCATGGATAGCTTAAACCTTAAATGGCTGGCATACTCCAATTT 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31600R-1.in silico 121 CAGGAGCTATAGTACATTCATGGATAGCTTAAACCTTAAATGGCTGGCATACTCCAATTT 180
31600R-1.IR_full 181 TAACGAGACAGAATGGCCTTCGATTGAAAATGCGGTTTTATGCTGTGGACTGGAGGGTCC 240
|||||||||||| |||||||||||||||||||||||||||||||||||||||||||||||
31600R-1.in silico 181 TAACGAGACAGATTGGCCTTCGATTGAAAATGCGGTTTTATGCTGTGGACTGGAGGGTCC 240
31600R-1.IR_full 241 CCGTTCTTATATGGATTATCTACAAGGGGTTCCAACCCACTGCTACCATCCCGACCTTAT 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31600R-1.in silico 241 CCGTTCTTATATGGATTATCTACAAGGGGTTCCAACCCACTGCTACCATCCCGACCTTAT 300
31600R-1.IR_full 301 CACCCAAGGTTGCAGTGACTTTGTTAAAAACATTGTTATGCCGATACAACAAATAAGTCA 360
|||||||||||||||||||||||||||||||||| |||||||||||||||| ||||||||
31600R-1.in silico 301 CACCCAAGGTTGCAGTGACTTTGTTAAAAACATTTTTATGCCGATACAACATATAAGTCA 360
31600R-1.IR_full 361 TTTGCAGCTCAGATTGGCTATTTTTGTGGAACTGGTAATTTTACTTATTCTTGCTGCAAC 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31600R-1.in silico 361 TTTGCAGCTCAGATTGGCTATTTTTGTGGAACTGGTAATTTTACTTATTCTTGCTGCAAC 420
31600R-1.IR_full 421 GCTTTTGAAGAAATGTATTTCTTTGATCGGCGAAAGGAAACATAAAAGAGTTATTACACA 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31600R-1.in silico 421 GCTTTTGAAGAAATGTATTTCTTTGATCGGCGAAAGGAAACATAAAAGAGTTATTACACA 480
31600R-1.IR_full 481 CGGGCTTGCGGTACTAATAG 500
||||||||||||||||||||
31600R-1.in silico 481 CGGGCTTGCGGTACTAATAG 500
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_165401.1 |
CG31600-RA (CG31600), mRNA |
| 0 |
0 |
2 |
4 |
2 |
NM_165139.1 |
CG4793-RC, transcript variant C (CG4793), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_141410.1 |
CG1138-RA (CG1138), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_137806.2 |
CG5465-RA (MED16), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_079799.2 |
CG6338-RA (Ets97D), mRNA |
| 0 |
0 |
0 |
6 |
4 |
NM_141786.2 |
CG4757-RA (CG4757), mRNA |
| 0 |
0 |
0 |
6 |
2 |
NM_141877.1 |
CG18347-RA (CG18347), mRNA |
| 0 |
0 |
0 |
4 |
7 |
NM_170414.1 |
CG2304-RB, transcript variant B (Trc8), mRNA |
| 0 |
0 |
0 |
4 |
7 |
NM_170413.2 |
CG2304-RA, transcript variant A (Trc8), mRNA |
| 0 |
0 |
0 |
4 |
7 |
NM_206580.1 |
CG2304-RD, transcript variant D (Trc8), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_135910.1 |
CG15256-RA (CG15256), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_137328.2 |
CG9010-RA (CG9010), mRNA |
| 0 |
0 |
0 |
3 |
9 |
NM_168486.1 |
CG6928-RA, transcript variant A (CG6928), mRNA |
| 0 |
0 |
0 |
3 |
6 |
NM_136011.2 |
CG7527-RA, transcript variant A (CadN2), mRNA |
| 0 |
0 |
0 |
3 |
6 |
NM_139882.1 |
CG7716-RA (CG7716), mRNA |
| 0 |
0 |
0 |
3 |
6 |
NM_165099.1 |
CG7595-RA, transcript variant A (ck), mRNA |
| 0 |
0 |
0 |
3 |
6 |
NM_001042903.1 |
CG7527-RB, transcript variant B (CadN2), mRNA |
| 0 |
0 |
0 |
3 |
6 |
NM_078847.2 |
CG7595-RB, transcript variant B (ck), mRNA |
| 0 |
0 |
0 |
3 |
5 |
NM_057815.3 |
CG5475-RA, transcript variant A (Mpk2), mRNA |
| 0 |
0 |
0 |
3 |
5 |
NM_170126.2 |
CG5475-RB, transcript variant B (Mpk2), mRNA |
| 0 |
0 |
0 |
3 |
4 |
NM_134871.1 |
CG3131-RA (Duox), mRNA |
| 0 |
0 |
0 |
3 |
4 |
NM_169826.1 |
CG31224-RA (CG31224), mRNA |
| 0 |
0 |
0 |
3 |
4 |
NM_167966.1 |
CG2103-RB, transcript variant B (pgant6), mRNA |
| 0 |
0 |
0 |
3 |
3 |
NM_170405.1 |
CG11956-RB, transcript variant B (SP1029), mRNA |
| 0 |
0 |
0 |
3 |
3 |
NM_167411.1 |
CG9413-RB, transcript variant B (CG9413), mRNA |
| 0 |
0 |
0 |
3 |
3 |
NM_132738.1 |
CG9413-RA, transcript variant A (CG9413), mRNA |
| 0 |
0 |
0 |
3 |
3 |
NM_144361.1 |
CG11956-RA, transcript variant A (SP1029), mRNA |
| 0 |
0 |
0 |
3 |
3 |
NM_170406.1 |
CG11956-RC, transcript variant C (SP1029), mRNA |
| 0 |
0 |
0 |
3 |
3 |
NM_132267.4 |
CG12109-RB (Caf1-180), mRNA |
| 0 |
0 |
0 |
3 |
3 |
NM_165202.1 |
CG31806-RA (CG31806), mRNA |
|
| Wing Disc |
GFP
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Puc
 |
Caspase
 |
DAPI
 |
Merge
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Figure
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JNK signal
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Caspase-3 signal
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