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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 31538R-1 
 Symbol CG31538  Full Name CG31538 
 CG No CG31538  Old CG No CG31538 
 Synonyms NEST:bs10d08, NEST:bs09e09, NEST:bs09g01, NEST:bs02h07, BcDNA:AT27831, BEST:GH11373, BEST:GH27336, BEST:LP11873, NEST:bs26g11, BEST:GH20014, CG31538 
 Accession No (Link to NCBI) NM_169046.1 
 Inserted Chr. lll 
 Insertional Mutation  semi-lethal 
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]  
 Comment balanced with TM6B, Tb 
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgGGCTTCAGCCTGCGGATGAAT 
 Primer Seq. 3' 
aatctagaggtaccGTAAGCATGTCGCGCGTGTCG 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 GGCTTCAGCC TGCGGATGAA TCCGCTGGCC GGCACACTGC AAAGGCTTTA CCACACCCGT 
0061 AGCAGGCCAT GTGTGGCCCA GAGTGTGAGC CAGGTGGGTG AACGGTCCAA GCCAACGTTG 
0121 CAGCCCTACG ACTTCGACCG AAGCCGGGTG CGTAAAATTG CCGGCGAGAG GAAGACGCTG 
0181 GTCGACCAGG TGCGGGGCAT GAGCACAAGC CTGGGACATG CCAAGTTGGT GCCCCAAAAG 
0241 ATGTACGGCA AGACGAGGCT GTTCTCCTCC GGAAGCACCC TCTACAGTCG CTTGCAGCAT 
0301 CTGGAACAGC AGAACGAGGA ATATTCGAGT CGAGAGTCCC AGCCGACGCA CACCACAGCC 
0361 TCATTGTCGG CCACCCAGCA GAGGTACAAT CAGGCGGCAG GATTCGGCAA GGGCATGGCC 
0421 ATACAGACGG CCAAAGACAT CAGCCAGTCC ATCCAGGAGG AGCTGATGGT GGTGGACGCC 
0481 GACACGCGCG ACATGCTTAC  
 in silico PCR Fragment 
0001 GGCTTCAGCC TGCGGATGAA TCCGCTGGCC GGCACACTGC AAAGGCTTTA CCACACCCGT 
0061 AGCAGGCCAT GTGTGGCCCA GAGTGTGAGC CAGGTGGGTG AACGGTCCAA GCCAACGTTG 
0121 CAGCCCTACG ACTTCGACCG AAGCCGGGTG CGTAAAATTG CCGGCGAGAG GAAGACGCTG 
0181 GTCGACCAGG TGCGGGGCAT GAGCACAAGC CTGGGACATG CCAAGTTGGT GCCCCAAAAG 
0241 ATGTACGGCA AGACGAGGCT GTTCTCCTCC GGAAGCACCC TCTACAGTCG CTTGCAGCAT 
0301 CTGGAACAGC AGAACGAGGA ATATTCGAGT CGAGAGTCCC AGCCGACGCA CACCACAGCC 
0361 TCATTGTCGG CCACCCAGCA GAGGTACAAT CAGGCGGCAG GATTCGGCAA GGGCATGGCC 
0421 ATACAGACGG CCAAAGACAT CAGCCAGTCC ATCCAGGAGG AGCTGATGGT GGTGGACGCC 
0481 GACACGCGCG ACATGCTTAC  
 Assemble Data 

31538R-1.IR_full       1   GGCTTCAGCCTGCGGATGAATCCGCTGGCCGGCACACTGCAAAGGCTTTACCACACCCGT 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31538R-1.in silico     1   GGCTTCAGCCTGCGGATGAATCCGCTGGCCGGCACACTGCAAAGGCTTTACCACACCCGT 60


31538R-1.IR_full       61  AGCAGGCCATGTGTGGCCCAGAGTGTGAGCCAGGTGGGTGAACGGTCCAAGCCAACGTTG 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31538R-1.in silico     61  AGCAGGCCATGTGTGGCCCAGAGTGTGAGCCAGGTGGGTGAACGGTCCAAGCCAACGTTG 120


31538R-1.IR_full       121 CAGCCCTACGACTTCGACCGAAGCCGGGTGCGTAAAATTGCCGGCGAGAGGAAGACGCTG 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31538R-1.in silico     121 CAGCCCTACGACTTCGACCGAAGCCGGGTGCGTAAAATTGCCGGCGAGAGGAAGACGCTG 180


31538R-1.IR_full       181 GTCGACCAGGTGCGGGGCATGAGCACAAGCCTGGGACATGCCAAGTTGGTGCCCCAAAAG 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31538R-1.in silico     181 GTCGACCAGGTGCGGGGCATGAGCACAAGCCTGGGACATGCCAAGTTGGTGCCCCAAAAG 240


31538R-1.IR_full       241 ATGTACGGCAAGACGAGGCTGTTCTCCTCCGGAAGCACCCTCTACAGTCGCTTGCAGCAT 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31538R-1.in silico     241 ATGTACGGCAAGACGAGGCTGTTCTCCTCCGGAAGCACCCTCTACAGTCGCTTGCAGCAT 300


31538R-1.IR_full       301 CTGGAACAGCAGAACGAGGAATATTCGAGTCGAGAGTCCCAGCCGACGCACACCACAGCC 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31538R-1.in silico     301 CTGGAACAGCAGAACGAGGAATATTCGAGTCGAGAGTCCCAGCCGACGCACACCACAGCC 360


31538R-1.IR_full       361 TCATTGTCGGCCACCCAGCAGAGGTACAATCAGGCGGCAGGATTCGGCAAGGGCATGGCC 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31538R-1.in silico     361 TCATTGTCGGCCACCCAGCAGAGGTACAATCAGGCGGCAGGATTCGGCAAGGGCATGGCC 420


31538R-1.IR_full       421 ATACAGACGGCCAAAGACATCAGCCAGTCCATCCAGGAGGAGCTGATGGTGGTGGACGCC 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31538R-1.in silico     421 ATACAGACGGCCAAAGACATCAGCCAGTCCATCCAGGAGGAGCTGATGGTGGTGGACGCC 480


31538R-1.IR_full       481 GACACGCGCGACATGCTTAC 500
                           ||||||||||||||||||||
31538R-1.in silico     481 GACACGCGCGACATGCTTAC 500



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_169046.1  CG31538-RA (CG31538), mRNA 
0.41   2  2  2  2  NM_138079.1  CG13582-RA (CG13582), mRNA 
0   0  4  3  8  NM_135559.2  CG5337-RA (CG5337), mRNA 
0   0  2  3  3  NM_135485.4  CG4619-RA (CG4619), mRNA 
0   0  2  2  5  NM_168199.1  CG8606-RB, transcript variant B (RhoGEF4), mRNA 
0   0  2  2  5  NM_139844.2  CG8606-RA, transcript variant A (RhoGEF4), mRNA 
0   0  2  2  3  NM_143455.1  CG15505-RA (Obp99d), mRNA 
0   0  2  2  2  NM_176581.2  CG33204-RA (CG33204), mRNA 
0   0  1  5  4  NM_079461.2  CG5219-RA (mRpL15), mRNA 
0   0  1  3  5  NM_057304.3  CG11988-RA, transcript variant A (neur), mRNA 
0   0  1  3  4  NM_206045.1  CG3572-RC, transcript variant C (vimar), mRNA 
0   0  1  3  4  NM_057957.4  CG3572-RB, transcript variant B (vimar), mRNA 
0   0  1  3  4  NM_169257.1  CG11988-RB, transcript variant B (neur), mRNA 
0   0  1  3  3  NM_167374.1  CG32632-RB (Tango13), mRNA 
0   0  1  3  2  NM_079090.2  CG3668-RA (fd59A), mRNA 
0   0  1  2  10  NM_167844.1  CG32479-RA (CG32479), mRNA 
0   0  1  2  5  NM_139904.1  CG8281-RA (CG8281), mRNA 
0   0  1  2  2  NM_164954.1  CG16833-RC, transcript variant C (CG16833), mRNA 
0   0  1  2  2  NM_143798.2  CG16833-RA, transcript variant A (CG16833), mRNA 
0   0  1  2  2  NM_164953.1  CG16833-RB, transcript variant B (CG16833), mRNA 
0   0  1  2  2  NM_142316.1  CG14906-RA (CG14906), mRNA 
0   0  0  6  4  NM_001032399.1  CG33955-RB (eys), mRNA 
0   0  0  5  9  NM_165760.2  CG18408-RB, transcript variant B (CAP), mRNA 
0   0  0  5  7  NM_001031917.1  CG1414-RB, transcript variant B (bbx), mRNA 
0   0  0  5  7  NM_001031918.1  CG1410-RA, transcript variant A (waw), mRNA 
0   0  0  5  7  NM_001043070.1  CG34146-RA (brp), mRNA 
0   0  0  5  4  NM_001014630.1  CG33547-RA (Rim), mRNA 
0   0  0  5  3  NM_001031916.1  CG1414-RC, transcript variant C (bbx), mRNA 
0   0  0  5  3  NM_057364.3  CG9999-RA (RanGap), mRNA 
0   0  0  5  3  NM_130511.1  CG14632-RB, transcript variant B (CG14632), mRNA 

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