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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 3151R-1 
 Symbol Rbp9  Full Name RNA-binding protein 9 
 CG No CG3151  Old CG No CG3151 
 Synonyms fs(2)B, fes, rbp9, RBP9, RRM9, RRM10, RBP9-2, rbp9a, rbp, rrm9, rrm10, Rbp10, CG15402, CG3151, Rbp9 
 Accession No (Link to NCBI) NM_057589.2 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Cho Y, Lai CM, Lin KY, Hsu HJ.
A Targeted RNAi Screen Reveals Drosophila Female-Sterile Genes That Control the Size of Germline Stem Cell Niche During Development.
G3 (Bethesda) (2018) 8(7) 2345-2354 [ PubMed ID = 29764959 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgGCAGCAGCAGCAGCAACCATC 
 Primer Seq. 3' 
aatctagaggtaccGCCTGAGGCCGTTCAATGCGT 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 GCAGCAGCAG CAGCAACCAT CCGGCGCGGG CgGTGcCAGN GAGCGTGTGG AAGCACCACC 
0061 GGATCCGCTG GAGGACCGGC TACgGCCAAC AATGTGACCA ACAGTCAGGC CCAAACCAAT 
0121 GGGGGCAcCA CTGCGACCAC GACTGCCGCA gCGGGTGCCG GATCCACGAC AAATGCGGCC 
0181 GTcggCCAGG CCACAGCAAA CAATGCTGCG AGCAACAACA ACAATAATAA TAATAATACC 
0241 AACAACAACA ATAACAATAA CGCCACTGCA AACAACAACA ACAATAATGA GCCAGACCCC 
0301 AAGACCAATT TGATTGTGAA CTACTTGCCA CAGACGATGT CGCAGGACGA GATCCGTTCG 
0361 TTGTTCGTCA GTTTTGGCGA GGTGGAGAGC TGCAAGTTGA TACGCGACAA GGTGACAGGA 
0421 CAAAGTCTGG GCTACGGATT CGTGAACTAC GTGAAGCAGG AGGATGCGGA AAAGGCAATC 
0481 AACGCATTGA ACGGCCTCAG CGGTAGC 
 in silico PCR Fragment 
0001 GCAGCAGCAG CAGCAACCAT CCGGCGCGGG CGGTGCCAGC G-GCGTG-GG AAGCACCACC 
0061 GGATCCGCTG GAGGACCGGC TACGGCCAAC AATGTGACCA ACAGTCAGGC CCAAACCAAT 
0121 GGGGGCACCA CTGCGACCAC GACTGCCGCA GCGGGTGCCG GATCCACGAC AAATGCGGCC 
0181 GTCGGCCAGG CCACAGCAAA CAATGCTGCG AGCAACAACA ACAATAATAA TAATAATACC 
0241 AACAACAACA ATAACAATAA CGCCACTGCA AACAACAACA ACAATAATGA GCCAGACCCC 
0301 AAGACCAATT TGATTGTGAA CTACTTGCCA CAGACGATGT CGCAGGACGA GATCCGTTCG 
0361 TTGTTCGTCA GTTTTGGCGA GGTGGAGAGC TGCAAGTTGA TACGCGACAA GGTGACAGGA 
0421 CAAAGTCTGG GCTACGGATT CGTGAACTAC GTGAAGCAGG AGGATGCGGA AAAGGCAATC 
0481 AACGCATTGA ACGGCCTCAG -----GC 
 Assemble Data 

3151R-1.IR_full       1   GCAGCAGCAGCAGCAACCATCCGGCGCGGGCGGTGCCAGNGAGCGTGTGGAAGCACCACC 60
                          ||||||||||||||||||||||||||||||||||||||| | ||||| ||||||||||||
3151R-1.in silico     1   GCAGCAGCAGCAGCAACCATCCGGCGCGGGCGGTGCCAGCG-GCGTG-GGAAGCACCACC 60


3151R-1.IR_full       61  GGATCCGCTGGAGGACCGGCTACGGCCAACAATGTGACCAACAGTCAGGCCCAAACCAAT 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3151R-1.in silico     61  GGATCCGCTGGAGGACCGGCTACGGCCAACAATGTGACCAACAGTCAGGCCCAAACCAAT 120


3151R-1.IR_full       121 GGGGGCACCACTGCGACCACGACTGCCGCAGCGGGTGCCGGATCCACGACAAATGCGGCC 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3151R-1.in silico     121 GGGGGCACCACTGCGACCACGACTGCCGCAGCGGGTGCCGGATCCACGACAAATGCGGCC 180


3151R-1.IR_full       181 GTCGGCCAGGCCACAGCAAACAATGCTGCGAGCAACAACAACAATAATAATAATAATACC 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3151R-1.in silico     181 GTCGGCCAGGCCACAGCAAACAATGCTGCGAGCAACAACAACAATAATAATAATAATACC 240


3151R-1.IR_full       241 AACAACAACAATAACAATAACGCCACTGCAAACAACAACAACAATAATGAGCCAGACCCC 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3151R-1.in silico     241 AACAACAACAATAACAATAACGCCACTGCAAACAACAACAACAATAATGAGCCAGACCCC 300


3151R-1.IR_full       301 AAGACCAATTTGATTGTGAACTACTTGCCACAGACGATGTCGCAGGACGAGATCCGTTCG 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3151R-1.in silico     301 AAGACCAATTTGATTGTGAACTACTTGCCACAGACGATGTCGCAGGACGAGATCCGTTCG 360


3151R-1.IR_full       361 TTGTTCGTCAGTTTTGGCGAGGTGGAGAGCTGCAAGTTGATACGCGACAAGGTGACAGGA 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3151R-1.in silico     361 TTGTTCGTCAGTTTTGGCGAGGTGGAGAGCTGCAAGTTGATACGCGACAAGGTGACAGGA 420


3151R-1.IR_full       421 CAAAGTCTGGGCTACGGATTCGTGAACTACGTGAAGCAGGAGGATGCGGAAAAGGCAATC 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3151R-1.in silico     421 CAAAGTCTGGGCTACGGATTCGTGAACTACGTGAAGCAGGAGGATGCGGAAAAGGCAATC 480


3151R-1.IR_full       481 AACGCATTGAACGGCCTCAGCGGTAGC 507
                          ||||||||||||||||||||     ||
3151R-1.in silico     481 AACGCATTGAACGGCCTCAG-----GC 507



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  16  46  130  NM_134299.3  CG3151-RF, transcript variant F (Rbp9), mRNA 
100   482  16  46  130  NM_134300.3  CG3151-RC, transcript variant C (Rbp9), mRNA 
100   482  16  46  123  NM_001014462.1  CG3151-RG, transcript variant G (Rbp9), mRNA 
100   482  16  46  123  NM_057589.2  CG3151-RA, transcript variant A (Rbp9), mRNA 
100   482  16  46  123  NM_057588.2  CG3151-RD, transcript variant D (Rbp9), mRNA 
100   482  16  46  123  NM_134297.3  CG3151-RB, transcript variant B (Rbp9), mRNA 
100   482  16  46  123  NM_134298.3  CG3151-RE, transcript variant E (Rbp9), mRNA 
2.07   10  49  118  182  NM_141121.2  CG7139-RA, transcript variant A (CG7139), mRNA 
2.07   10  15  40  76  NM_143459.1  CG1964-RA (Kul), mRNA 
1.86   9  20  40  99  NM_057368.3  CG2621-RD, transcript variant D (sgg), mRNA 
1.24   6  37  92  118  NM_166999.2  CG32790-RA (CG32790), mRNA 
1.24   6  13  25  51  NM_078514.2  CG9653-RA (brk), mRNA 
1.03   5  29  77  154  NM_001038965.1  CG9924-RE, transcript variant E (CG9924), mRNA 
1.03   5  25  64  125  NM_142068.1  CG9924-RC, transcript variant C (CG9924), mRNA 
1.03   5  25  64  125  NM_169564.1  CG9924-RD, transcript variant D (CG9924), mRNA 
1.03   5  25  64  125  NM_169565.1  CG9924-RA, transcript variant A (CG9924), mRNA 
1.03   5  25  64  125  NM_169566.1  CG9924-RB, transcript variant B (CG9924), mRNA 
1.03   5  13  42  82  NM_080331.2  CG3578-RA (bi), mRNA 
0.82   4  33  84  150  NM_164741.1  CG4502-RB, transcript variant B (CG4502), mRNA 
0.82   4  33  84  150  NM_135259.2  CG4502-RA, transcript variant A (CG4502), mRNA 
0.82   4  24  109  248  NM_079002.2  CG3905-RA (Su(z)2), mRNA 
0.82   4  21  76  155  NM_134554.1  CG15455-RA (CG15455), mRNA 
0.82   4  19  35  47  NM_142931.3  CG10192-RA (CG10192), mRNA 
0.82   4  17  53  131  NM_079224.3  CG10037-RA (vvl), mRNA 
0.82   4  16  30  53  NM_165915.1  CG8815-RB, transcript variant B (Sin3A), mRNA 
0.82   4  16  30  53  NM_165916.1  CG8815-RC, transcript variant C (Sin3A), mRNA 
0.82   4  16  30  53  NM_136955.2  CG8815-RA, transcript variant A (Sin3A), mRNA 
0.82   4  14  45  110  NM_206091.1  CG7734-RD, transcript variant D (shn), mRNA 
0.82   4  14  45  110  NM_057376.2  CG7734-RC, transcript variant C (shn), mRNA 
0.82   4  14  45  109  NM_057375.3  CG7734-RA, transcript variant A (shn), mRNA 

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