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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 31496R-2 
 Symbol CG31496  Full Name CG31496 
 CG No CG31496  Old CG No CG31496 
 Synonyms CG14604, CG31496 
 Accession No (Link to NCBI) NM_169182.1 
 Inserted Chr. ll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgGTGTGGGTCTTTTGGGTGGAG 
 Primer Seq. 3' 
aatctagaggtaccAGGAACAACAGAAGGGGCAGC 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 GTGTGGGTCT TTTGGGTGGA GCTTTAgCTG GCGCTGTCAT TGGCAACGTT GTGGCCAAGT 
0061 CTCAACNGGC NCCCGNTGCC GCTCCTgCTG CTCCGGTAGC CGTAGTGNAT CACCACTATC 
0121 CGCCAgGTGC TCCTGCAGCA CNTGCTCCGG CATCCCAGCC AGATCCCAAA AAGATGACTG 
0181 TCATCGANAA CTGGCACACC CGATGCCAAC GGATGCTACA CGCAAACTAT TCGGGAACCC 
0241 AATCCCAGCA ATCCAAAGTC CTATACGGAG ACACAGCACC TGATCTGTNC CCACGCTGCA 
0301 GCAGGCCAAC CAGCCTGCAC CAGCAATCGC TCCAAGTATT GCCCAGGTTC CTGTGATGCC 
0361 GCAGCCCTCA CCAGTTTTCC CTGCGGTATC TCCAGCTGCT GCTCCGGCCA ATGTTGCTCC 
0421 AGCTCCTGCT GCTCCTGTNN CTCCGGCTGC CCCAGCNGCC CCTGTGGTGC CTGTCGCTCC 
0481 AGCTGCCCCT TCTGTTGTTN CCT 
 in silico PCR Fragment 
0001 GTGTGGGTCT TTTGGGTGGA GCTTTAGCTG GCGCTGTCAT TGGCAACGTT GTGGCCAAGT 
0061 CTCAACAGGC TCCCGCTGCC GCTCCTGCTG CTCCGGTAGC CGTAGTGCAT CACCACTATC 
0121 CGCCAGGTGC TCCTGCAGCA CCTGCTCCGG CATCCCAGCC AGATCCCAAA AAGATGACTG 
0181 TCATCGA-AA CTGGCACACC CGATGCCAAC GGATGCTACA CGCAAACTAT TCGGGAACCC 
0241 AATCCCAGCA ATCCAAAGTC CTATACGGAG ACACAGCACC TGATCTGT-C CCACGCTGCA 
0301 GCAGGCCAAC CAGCCTGCAC CAGCAATCGC TCCAAGTATT GCCCAGGTTC CTGTGATGCC 
0361 GCAGCCCTCA CCAGTTTTCC CTGCGGTATC TCCAGCTGCT GCTCCGGCCA ATGTTGCTCC 
0421 AGCTCCTGCT GCTCCTGTTG CTCCGGCTGC CCCAGCTGCC CCTGTGGTGC CTGTCGCTCC 
0481 AGCTGCCCCT TCTGTTGTT- CCT 
 Assemble Data 

31496R-2.IR_full       1   GTGTGGGTCTTTTGGGTGGAGCTTTAGCTGGCGCTGTCATTGGCAACGTTGTGGCCAAGT 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31496R-2.in silico     1   GTGTGGGTCTTTTGGGTGGAGCTTTAGCTGGCGCTGTCATTGGCAACGTTGTGGCCAAGT 60


31496R-2.IR_full       61  CTCAACNGGCNCCCGNTGCCGCTCCTGCTGCTCCGGTAGCCGTAGTGNATCACCACTATC 120
                           |||||| ||| |||| ||||||||||||||||||||||||||||||| ||||||||||||
31496R-2.in silico     61  CTCAACAGGCTCCCGCTGCCGCTCCTGCTGCTCCGGTAGCCGTAGTGCATCACCACTATC 120


31496R-2.IR_full       121 CGCCAGGTGCTCCTGCAGCACNTGCTCCGGCATCCCAGCCAGATCCCAAAAAGATGACTG 180
                           ||||||||||||||||||||| ||||||||||||||||||||||||||||||||||||||
31496R-2.in silico     121 CGCCAGGTGCTCCTGCAGCACCTGCTCCGGCATCCCAGCCAGATCCCAAAAAGATGACTG 180


31496R-2.IR_full       181 TCATCGANAACTGGCACACCCGATGCCAACGGATGCTACACGCAAACTATTCGGGAACCC 240
                           ||||||| ||||||||||||||||||||||||||||||||||||||||||||||||||||
31496R-2.in silico     181 TCATCGA-AACTGGCACACCCGATGCCAACGGATGCTACACGCAAACTATTCGGGAACCC 240


31496R-2.IR_full       241 AATCCCAGCAATCCAAAGTCCTATACGGAGACACAGCACCTGATCTGTNCCCACGCTGCA 300
                           |||||||||||||||||||||||||||||||||||||||||||||||| |||||||||||
31496R-2.in silico     241 AATCCCAGCAATCCAAAGTCCTATACGGAGACACAGCACCTGATCTGT-CCCACGCTGCA 300


31496R-2.IR_full       301 GCAGGCCAACCAGCCTGCACCAGCAATCGCTCCAAGTATTGCCCAGGTTCCTGTGATGCC 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31496R-2.in silico     301 GCAGGCCAACCAGCCTGCACCAGCAATCGCTCCAAGTATTGCCCAGGTTCCTGTGATGCC 360


31496R-2.IR_full       361 GCAGCCCTCACCAGTTTTCCCTGCGGTATCTCCAGCTGCTGCTCCGGCCAATGTTGCTCC 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31496R-2.in silico     361 GCAGCCCTCACCAGTTTTCCCTGCGGTATCTCCAGCTGCTGCTCCGGCCAATGTTGCTCC 420


31496R-2.IR_full       421 AGCTCCTGCTGCTCCTGTNNCTCCGGCTGCCCCAGCNGCCCCTGTGGTGCCTGTCGCTCC 480
                           ||||||||||||||||||  |||||||||||||||| |||||||||||||||||||||||
31496R-2.in silico     421 AGCTCCTGCTGCTCCTGTTGCTCCGGCTGCCCCAGCTGCCCCTGTGGTGCCTGTCGCTCC 480


31496R-2.IR_full       481 AGCTGCCCCTTCTGTTGTTNCCT 503
                           ||||||||||||||||||| |||
31496R-2.in silico     481 AGCTGCCCCTTCTGTTGTT-CCT 503



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  21  50  NM_169182.1  CG31496-RA (CG31496), mRNA 
0.41   2  3  2  5  NM_136198.2  CG2608-RA (CG2608), mRNA 
0.41   2  2  9  17  NM_136807.1  CG13226-RA (CG13226), mRNA 
0.2   1  2  4  13  NM_142104.1  CG14847-RA (CG14847), mRNA 
0   0  9  13  17  NM_176001.1  CG32982-RA, transcript variant A (CG32982), mRNA 
0   0  9  12  34  NM_142851.1  CG13835-RA (CG13835), mRNA 
0   0  8  18  74  NM_143148.1  CG5079-RA (CG5079), mRNA 
0   0  7  9  42  NM_169223.2  CG31369-RA (PQBP-1), mRNA 
0   0  6  35  143  NM_167319.3  CG32656-RA (CG32656), mRNA 
0   0  6  23  41  NM_169177.1  CG2507-RB, transcript variant B (sas), mRNA 
0   0  6  21  93  NM_143310.2  CG12885-RA (CG12885), mRNA 
0   0  6  15  128  NM_001014565.1  CG10491-RB, transcript variant B (vn), mRNA 
0   0  6  15  128  NM_079218.2  CG10491-RA, transcript variant A (vn), mRNA 
0   0  6  3  5  NM_137567.1  CG9811-RA (Rgk1), mRNA 
0   0  5  6  23  NM_078573.2  CG1822-RC, transcript variant C (bif), mRNA 
0   0  5  6  23  NM_167295.1  CG1822-RB, transcript variant B (bif), mRNA 
0   0  4  16  51  NM_135505.2  CG4839-RA, transcript variant A (CG4839), mRNA 
0   0  4  16  51  NM_164890.1  CG4839-RB, transcript variant B (CG4839), mRNA 
0   0  4  12  55  NM_130497.2  CG13363-RA (Suv4-20), mRNA 
0   0  4  11  65  NM_132457.1  CG11122-RA (CG11122), mRNA 
0   0  4  9  86  NM_132288.1  CG15365-RA (CG15365), mRNA 
0   0  4  6  29  NM_140725.1  CG18265-RA (CG18265), mRNA 
0   0  4  6  16  NM_165112.1  CG31829-RA (CG31829), mRNA 
0   0  3  17  87  NM_137342.2  CG30460-RC, transcript variant C (CG30460), mRNA 
0   0  3  17  57  NM_167844.1  CG32479-RA (CG32479), mRNA 
0   0  3  13  80  NM_206150.1  CG30460-RB, transcript variant B (CG30460), mRNA 
0   0  3  13  80  NM_206149.1  CG30460-RA, transcript variant A (CG30460), mRNA 
0   0  3  12  22  NM_169541.1  CG9374-RA, transcript variant A (lkb1), mRNA 
0   0  3  12  22  NM_169544.1  CG9374-RE, transcript variant E (lkb1), mRNA 
0   0  3  12  22  NM_169547.1  CG9374-RI, transcript variant I (lkb1), mRNA 

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