NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 31347R-3

Symbol CG31347 Full Name CG31347

CG No CG31347 Old CG No CG31347

Synonyms CG31347

Accession No (Link to NCBI) NM_169477.1

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
[Please submit your publication] Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGAATCCTTTAGTGTGCCGGAA

Primer Seq. 3'

aatctagaggtaccACACGCACACACGGATATCTG

Predicted Fragment Size

IR fragment full Seq

0001 GAATCCTTTA GTGTGCCGGA AGTGGACATG ATGACCGTGT CAAAGAATTC GCAATACGAA 
0061 CGGGAATCGC TACTAAAGTT CGCACCGCCA GTTGGACTGA CCAACATCAG CTACGGCAGC 
0121 TTGTACAAGG TGGACTCCTT CTATTTGGAG TGCCAGAAGT ACCGAGATCA ATTCCGCGAT 
0181 CCCTACAACA AGCTGCNTGC GCCCCAAGAT GTTCAGCACA TACAGGGGCA AGTGTGGTGT 
0241 GAAGATAGAT CCCGAACTGG AGCGCTTAAA GCGACCCGCT GCCTCCCATG TGGAGTCTAT 
0301 TCCCGTGGTT TACCCAATGG AGTACGGGCA CCAAATGGAC TTCGACAGAG GCTTCCAGAT 
0361 GCAGGGACGC AACAGCAGGG ATGCAGCCAC CGCCCGCAGA TATCCGTGTG TGCGTGT

in silico PCR Fragment

0001 GAATCCTTTA GTGTGCCGGA AGTGGACATG ATGACCGTGT CAAAGAATTC GCAATACGAA 
0061 CGGGAATCGC TACTAAAGTT CGCACCGCCA GTTGGACTGA CCAACATCAG CTACGGCAGC 
0121 TTGTACAAGG TGGACTCCTT CTATTTGGAG TGCCAGAAGT ACCGAGATCA ATTCCGCGAT 
0181 CCCTACAACA AGCTGC-TGC GCCCCAAGAT GTTCAGCACA TACAGGGGCA AGTGTGGTGT 
0241 GAAGATAGAT CCCGAACTGG AGCGCTTAAA GCGACCCGCT GCCTCCCATG TGGAGTCTAT 
0301 TCCCGTGGTT TACCCAATGG AGTACGGGCA CCAAATGGAC TTCGACAGAG GCTTCCAGAT 
0361 GCAGGGACGC AACAGCAGGG ATGCAGCCAC CGCCCGCAGA TATCCGTGTG TGCGTGT

Assemble Data


31347R-3.IR_full       1   GAATCCTTTAGTGTGCCGGAAGTGGACATGATGACCGTGTCAAAGAATTCGCAATACGAA 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31347R-3.in silico     1   GAATCCTTTAGTGTGCCGGAAGTGGACATGATGACCGTGTCAAAGAATTCGCAATACGAA 60


31347R-3.IR_full       61  CGGGAATCGCTACTAAAGTTCGCACCGCCAGTTGGACTGACCAACATCAGCTACGGCAGC 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31347R-3.in silico     61  CGGGAATCGCTACTAAAGTTCGCACCGCCAGTTGGACTGACCAACATCAGCTACGGCAGC 120


31347R-3.IR_full       121 TTGTACAAGGTGGACTCCTTCTATTTGGAGTGCCAGAAGTACCGAGATCAATTCCGCGAT 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31347R-3.in silico     121 TTGTACAAGGTGGACTCCTTCTATTTGGAGTGCCAGAAGTACCGAGATCAATTCCGCGAT 180


31347R-3.IR_full       181 CCCTACAACAAGCTGCNTGCGCCCCAAGATGTTCAGCACATACAGGGGCAAGTGTGGTGT 240
                           |||||||||||||||| |||||||||||||||||||||||||||||||||||||||||||
31347R-3.in silico     181 CCCTACAACAAGCTGC-TGCGCCCCAAGATGTTCAGCACATACAGGGGCAAGTGTGGTGT 240


31347R-3.IR_full       241 GAAGATAGATCCCGAACTGGAGCGCTTAAAGCGACCCGCTGCCTCCCATGTGGAGTCTAT 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31347R-3.in silico     241 GAAGATAGATCCCGAACTGGAGCGCTTAAAGCGACCCGCTGCCTCCCATGTGGAGTCTAT 300


31347R-3.IR_full       301 TCCCGTGGTTTACCCAATGGAGTACGGGCACCAAATGGACTTCGACAGAGGCTTCCAGAT 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31347R-3.in silico     301 TCCCGTGGTTTACCCAATGGAGTACGGGCACCAAATGGACTTCGACAGAGGCTTCCAGAT 360


31347R-3.IR_full       361 GCAGGGACGCAACAGCAGGGATGCAGCCACCGCCCGCAGATATCCGTGTGTGCGTGT 417
                           |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31347R-3.in silico     361 GCAGGGACGCAACAGCAGGGATGCAGCCACCGCCCGCAGATATCCGTGTGTGCGTGT 417

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	398	0	0	0	NM_169477.1	CG31347-RA (CG31347), mRNA
0	0	9	11	4	NM_141968.3	CG14391-RA (CG14391), mRNA
0	0	2	4	5	NM_132184.1	CG15327-RA (CG15327), mRNA
0	0	1	6	4	NM_135801.2	CG9302-RA (CG9302), mRNA
0	0	1	3	3	NM_138106.1	CG3640-RA (CG3640), mRNA
0	0	1	2	4	NM_137807.2	CG5625-RA, transcript variant A (CG5625), mRNA
0	0	1	2	4	NM_166518.1	CG5625-RB, transcript variant B (CG5625), mRNA
0	0	1	2	2	NM_136448.2	CG2144-RA (CG2144), mRNA
0	0	1	2	2	NM_136952.2	CG8587-RA (Cyp301a1), mRNA
0	0	0	7	2	NM_079131.2	CG3594-RA (Eap), mRNA
0	0	0	5	18	NM_168755.1	CG8127-RB, transcript variant B (Eip75B), mRNA
0	0	0	5	15	NM_168756.1	CG8127-RC, transcript variant C (Eip75B), mRNA
0	0	0	5	13	NM_168757.1	CG8127-RD, transcript variant D (Eip75B), mRNA
0	0	0	5	13	NM_079409.2	CG8127-RA, transcript variant A (Eip75B), mRNA
0	0	0	5	3	NM_137092.2	CG8331-RA (CG8331), mRNA
0	0	0	5	3	NM_001014605.1	CG1119-RB, transcript variant B (Gnf1), mRNA
0	0	0	5	3	NM_079505.2	CG1119-RA, transcript variant A (Gnf1), mRNA
0	0	0	4	12	NM_131948.1	CG3081-RA (CG3081), mRNA
0	0	0	4	5	NM_134817.1	CG4267-RA (CG4267), mRNA
0	0	0	4	4	NM_164903.1	CG18619-RB, transcript variant B (CG18619), mRNA
0	0	0	4	4	NM_135519.1	CG18619-RA, transcript variant A (CG18619), mRNA
0	0	0	4	2	NM_136452.2	CG1598-RA (CG1598), mRNA
0	0	0	4	2	NM_130594.1	CG14803-RA (CG14803), mRNA
0	0	0	3	8	NM_130636.2	CG3073-RA (l(1)G0144), mRNA
0	0	0	3	7	NM_135438.2	CG13110-RA (CG13110), mRNA
0	0	0	3	6	NM_206187.2	CG10036-RB, transcript variant B (otp), mRNA
0	0	0	3	6	NM_134965.2	CG3407-RA (CG3407), mRNA
0	0	0	3	6	NM_001043098.1	CG10036-RC, transcript variant C (otp), mRNA
0	0	0	3	6	NM_079075.4	CG10036-RA, transcript variant A (otp), mRNA
0	0	0	3	5	NM_144375.2	CG1652-RA (lectin-46Cb), mRNA

Wing Disc

GFP

Puc

Caspase

DAPI

Merge

Figure

JNK signal

Caspase-3 signal

Dcompartment		Penetrance

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