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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
31318R-1 |
| Symbol |
Rpb4 |
Full Name |
Rpb4 |
| CG No |
CG33520 |
Old CG No |
CG31318 |
| Synonyms |
Ada2a/Rpb4, RPB4, CG7150, Ada2a, dRpb4, dAda2a, ADA2A, dAda2A, ADA2a, CG31237, CG31318, CG33520, Ada2A, anon-WO0118547.402, Rpb4 |
| Accession No (Link to NCBI) |
NM_001014635.1 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Park J, Lee J, Kim JH, Lee J, Park H, Lim C.
ZNF598 co-translationally titrates poly(GR) protein implicated in the pathogenesis of C9ORF72-associated ALS/FTD.
Nucleic Acids Res (2021) 49(19) 11294-11311
[
PubMed ID = 34551427
]
[
RRC reference
]
Nitta Y, Yamazaki D, Sugie A, Hiroi M, Tabata T.
DISCO Interacting Protein 2 regulates axonal bifurcation and guidance of Drosophila mushroom body neurons.
Dev Biol (2017) 421(2) 233-244
[
PubMed ID = 27908785
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgCCTGCAGTTTCCCAAAGTTGA |
| Primer Seq. 3' |
aatctagaggtaccAGCAATCGTGGTAGTGACGAC |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 CCTGCAGTTT CCCAAAGTTG AGCCACTGGG GCCACAGGTT AAGCGGTTCA TCCATGCACA
0061 TCAGAATGAA CACACCAGGA ACTCCATGGT TTTGTATAAT CCGCACACGT TGCACAGGTA
0121 CTTGGAGGAG ATGGAGGAAA AGACGCGGGA CCAGAATAGC AGTGTTCCAT CGGCTACAAA
0181 GGATGCCAAT CGGTGTGCCA CATGTCGCTG CAGTCTCACA GAGCCGTACA TTAAGTGCTC
0241 CGAGTGCCTC GACACCCTGC TCTGCCTGCA ATGTTTCTCA CGTGGAAAGG AGGCCTTTTC
0301 TCACCGCAAT AACCACGCTT ACATCATAGT CCGCGACAAC ATACAGGTTT TCGCCGATGA
0361 GCCACACTGG ACGGCACGGG ACGANGCGCA TACTGCTGAA AACACTTCGC ACCCACGGCT
0421 ATGGCAACTG GGAGGCGGTC TCCCAGGCTC TGGACCAGCG CCACGAACCC GCAGAAGTGC
0481 GTCGTCACTA CCACGATTGC T |
| in silico PCR Fragment |
0001 CCTGCAGTTT CCCAAAGTTG AGCCACTGGG GCCACAGGTT AAGCGGTTCA TCCATGCACA
0061 TCAGAATGAA CACACCAGGA ACTCCATGGT TTTGTATAAT CCGCACACGT TGCACAGGTA
0121 CTTGGAGGAG ATGGAGGAAA AGACGCGGGA CCAGAATAGC AGTGTTCCAT CGGCTACAAA
0181 GGATGCCAAT CGGTGTGCCA CATGTCGCTG CAGTCTCACA GAGCCGTACA TTAAGTGCTC
0241 CGAGTGCCTC GACACCCTGC TCTGCCTGCA ATGTTTCTCA CGTGGAAAGG AGGCCTTTTC
0301 TCACCGCAAT AACCACGCTT ACATCATAGT CCGCGACAAC ATACAGGTTT TCGCCGATGA
0361 GCCACACTGG ACGGCACGGG ACGA-GCGCA TACTGCTGAA AACACTTCGC ACCCACGGCT
0421 ATGGCAACTG GGAGGCGGTC TCCCAGGCTC TGGACCAGCG CCACGAACCC GCAGAAGTGC
0481 GTCGTCACTA CCACGATTGC T |
| Assemble Data |
31318R-1.IR_full 1 CCTGCAGTTTCCCAAAGTTGAGCCACTGGGGCCACAGGTTAAGCGGTTCATCCATGCACA 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31318R-1.in silico 1 CCTGCAGTTTCCCAAAGTTGAGCCACTGGGGCCACAGGTTAAGCGGTTCATCCATGCACA 60
31318R-1.IR_full 61 TCAGAATGAACACACCAGGAACTCCATGGTTTTGTATAATCCGCACACGTTGCACAGGTA 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31318R-1.in silico 61 TCAGAATGAACACACCAGGAACTCCATGGTTTTGTATAATCCGCACACGTTGCACAGGTA 120
31318R-1.IR_full 121 CTTGGAGGAGATGGAGGAAAAGACGCGGGACCAGAATAGCAGTGTTCCATCGGCTACAAA 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31318R-1.in silico 121 CTTGGAGGAGATGGAGGAAAAGACGCGGGACCAGAATAGCAGTGTTCCATCGGCTACAAA 180
31318R-1.IR_full 181 GGATGCCAATCGGTGTGCCACATGTCGCTGCAGTCTCACAGAGCCGTACATTAAGTGCTC 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31318R-1.in silico 181 GGATGCCAATCGGTGTGCCACATGTCGCTGCAGTCTCACAGAGCCGTACATTAAGTGCTC 240
31318R-1.IR_full 241 CGAGTGCCTCGACACCCTGCTCTGCCTGCAATGTTTCTCACGTGGAAAGGAGGCCTTTTC 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31318R-1.in silico 241 CGAGTGCCTCGACACCCTGCTCTGCCTGCAATGTTTCTCACGTGGAAAGGAGGCCTTTTC 300
31318R-1.IR_full 301 TCACCGCAATAACCACGCTTACATCATAGTCCGCGACAACATACAGGTTTTCGCCGATGA 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31318R-1.in silico 301 TCACCGCAATAACCACGCTTACATCATAGTCCGCGACAACATACAGGTTTTCGCCGATGA 360
31318R-1.IR_full 361 GCCACACTGGACGGCACGGGACGANGCGCATACTGCTGAAAACACTTCGCACCCACGGCT 420
|||||||||||||||||||||||| |||||||||||||||||||||||||||||||||||
31318R-1.in silico 361 GCCACACTGGACGGCACGGGACGA-GCGCATACTGCTGAAAACACTTCGCACCCACGGCT 420
31318R-1.IR_full 421 ATGGCAACTGGGAGGCGGTCTCCCAGGCTCTGGACCAGCGCCACGAACCCGCAGAAGTGC 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31318R-1.in silico 421 ATGGCAACTGGGAGGCGGTCTCCCAGGCTCTGGACCAGCGCCACGAACCCGCAGAAGTGC 480
31318R-1.IR_full 481 GTCGTCACTACCACGATTGCT 501
|||||||||||||||||||||
31318R-1.in silico 481 GTCGTCACTACCACGATTGCT 501
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
2 |
NM_001014635.1 |
CG33520-RA, transcript variant A (Rpb4), mRNA |
| 100 |
482 |
0 |
0 |
2 |
NM_001014637.1 |
CG33520-RC, transcript variant C (Rpb4), mRNA |
| 96.88 |
467 |
3 |
2 |
5 |
NM_001014636.1 |
CG33520-RB, transcript variant B (Rpb4), mRNA |
| 96.88 |
467 |
3 |
2 |
5 |
NM_001014634.1 |
CG33520-RE, transcript variant E (Rpb4), mRNA |
| 0.2 |
1 |
2 |
3 |
3 |
NM_141426.1 |
CG2360-RA (Ccp84Aa), mRNA |
| 0.2 |
1 |
2 |
3 |
3 |
NM_141425.1 |
CG1252-RA (Ccp84Ab), mRNA |
| 0 |
0 |
3 |
3 |
3 |
NM_136502.2 |
CG14762-RA (CG14762), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_001032048.1 |
CG7254-RB, transcript variant B (GlyP), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_164453.3 |
CG7254-RA, transcript variant A (GlyP), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_137155.2 |
CG10228-RA (Pcf11), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_079054.3 |
CG6493-RA (Dcr-2), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_176777.1 |
CG14616-RC, transcript variant C (l(1)G0196), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_132155.2 |
CG4617-RA (CG4617), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_176780.1 |
CG14616-RE, transcript variant E (l(1)G0196), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_176779.1 |
CG14616-RA, transcript variant A (l(1)G0196), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_176778.1 |
CG14616-RD, transcript variant D (l(1)G0196), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_140314.2 |
CG10698-RA (GRHRII), mRNA |
| 0 |
0 |
0 |
6 |
4 |
NM_166465.1 |
CG9856-RA (PTP-ER), mRNA |
| 0 |
0 |
0 |
6 |
2 |
NM_140648.1 |
CG4169-RA (CG4169), mRNA |
| 0 |
0 |
0 |
6 |
2 |
NM_141982.2 |
CG7242-RA (CG7242), mRNA |
| 0 |
0 |
0 |
5 |
7 |
NM_057734.3 |
CG3924-RA, transcript variant A (Chi), mRNA |
| 0 |
0 |
0 |
5 |
7 |
NM_166650.1 |
CG3924-RB, transcript variant B (Chi), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_164701.1 |
CG11328-RA, transcript variant A (Nhe3), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_135220.2 |
CG11328-RB, transcript variant B (Nhe3), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_079478.3 |
CG5069-RA (croc), mRNA |
| 0 |
0 |
0 |
5 |
2 |
NM_138063.2 |
CG13578-RA (CG13578), mRNA |
| 0 |
0 |
0 |
4 |
9 |
NM_132410.2 |
CG2124-RA (CG2124), mRNA |
| 0 |
0 |
0 |
4 |
7 |
NM_139618.2 |
CG1259-RB (CG1259), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_170354.1 |
CG12428-RE, transcript variant E (CG12428), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_139532.1 |
CG14967-RA (CG14967), mRNA |
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