National Institute of Genetics
Contact us      Quick Search       
NIG-Fly - Fly Stocks of National Institute of Genetics (NIG) Drosophila strain -NIG-Fly - Fly Stocks of National Institute of Genetics (NIG) Drosophila strain -NIG-Fly - Fly Stocks of National Institute of Genetics (NIG) Drosophila strain -
Home Home
Japanese | English  
Notice:

Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
NIG-RNAi Strains Detail
Request : Click the "Order" button to request for this stock   request request_help.gif
Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 31287R-3 
 Symbol CG31287  Full Name CG31287 
 CG No CG31287  Old CG No CG31287 
 Synonyms NEST:bs24f08, CG31287 
 Accession No (Link to NCBI) NM_169708.1 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgTGCAGGACAGCCTTGAAATGG 
 Primer Seq. 3' 
aatctagaggtaccCTGCTCGAACTCCTTTTGAAC 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 TGCAGGACAG CCTTGAAATG GAATCAGATT TCAATACCAA GTTAAAATTT CTTAAGGAAC 
0061 AGGAACAAAT TTGCCTAGAC AATGCCAAAA ATGCACTTCC CATCGATGAT ACCCTGAGAT 
0121 CAGTAAAGTG CACCATGCTT AGGGTAGAAA TGGAGCTGCA TGAAAACGAG ATTAACCTTA 
0181 TACAGGCGGA GAAAGCAGTT ACGAAATTGG AGCAGAGCTA TCCGAGTCCT GAGAGTCTGG 
0241 CAGGCCAAGT GTTTCCTTTG GCAAGTACCA CATATCAGGA TTTGCTGAGT ATGCTGATGT 
0301 CTTCGAAAAA AACAGTCGAT CATTGCAATC AGATCAAAGA GGAGGTCGCG ACGTATAATG 
0361 AGCAGATATC CGCAAAAGTA CCTTCCGTAA ATTTGGTAAC CAAGCTTATC GATTACCACA 
0421 ATAATACACT GGAGTCTATG GAAAAAAATA TCGAAAAATT GAAGAGCCAG GTCACACAAG 
0481 TTCAAAAGGA GTTCGAGCAG  
 in silico PCR Fragment 
0001 TGCAGGACAG CCTTGAAATG GAATCAGATT TCAATACCAA GTTAAAATTT CTTAAGGAAC 
0061 AGGAACAAAT TTGCCTAGAC AATGCCAAAA ATGCACTTCC CATCGATGAT ACCCTGAGAT 
0121 CAGTAAAGTG CACCATGCTT AGGGTAGAAA TGGAGCTGCA TGAAAACGAG ATTAACCTTA 
0181 TACAGGCGGA GAAAGCAGTT ACGAAATTGG AGCAGAGCTA TCCGAGTCCT GAGAGTCTGG 
0241 CAGGCCAAGT GTTTCCTTTG GCAAGTACCA CATATCAGGA TTTGCTGAGT ATGCTGATGT 
0301 CTTCGAAAAA AACAGTCGAT CATTGCAATC AGATCAAAGA GGAGGTCGCG ACGTATAATG 
0361 AGCAGATATC CGCAAAAGTA CCTTCCGTAA ATTTGGTAAC CAAGCTTATC GATTACCACA 
0421 ATAATACACT GGAGTCTATG GAAAAAAATA TCGAAAAATT GAAGAGCCAG GTCACACAAG 
0481 TTCAAAAGGA GTTCGAGCAG  
 Assemble Data 

31287R-3.IR_full       1   TGCAGGACAGCCTTGAAATGGAATCAGATTTCAATACCAAGTTAAAATTTCTTAAGGAAC 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31287R-3.in silico     1   TGCAGGACAGCCTTGAAATGGAATCAGATTTCAATACCAAGTTAAAATTTCTTAAGGAAC 60


31287R-3.IR_full       61  AGGAACAAATTTGCCTAGACAATGCCAAAAATGCACTTCCCATCGATGATACCCTGAGAT 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31287R-3.in silico     61  AGGAACAAATTTGCCTAGACAATGCCAAAAATGCACTTCCCATCGATGATACCCTGAGAT 120


31287R-3.IR_full       121 CAGTAAAGTGCACCATGCTTAGGGTAGAAATGGAGCTGCATGAAAACGAGATTAACCTTA 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31287R-3.in silico     121 CAGTAAAGTGCACCATGCTTAGGGTAGAAATGGAGCTGCATGAAAACGAGATTAACCTTA 180


31287R-3.IR_full       181 TACAGGCGGAGAAAGCAGTTACGAAATTGGAGCAGAGCTATCCGAGTCCTGAGAGTCTGG 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31287R-3.in silico     181 TACAGGCGGAGAAAGCAGTTACGAAATTGGAGCAGAGCTATCCGAGTCCTGAGAGTCTGG 240


31287R-3.IR_full       241 CAGGCCAAGTGTTTCCTTTGGCAAGTACCACATATCAGGATTTGCTGAGTATGCTGATGT 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31287R-3.in silico     241 CAGGCCAAGTGTTTCCTTTGGCAAGTACCACATATCAGGATTTGCTGAGTATGCTGATGT 300


31287R-3.IR_full       301 CTTCGAAAAAAACAGTCGATCATTGCAATCAGATCAAAGAGGAGGTCGCGACGTATAATG 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31287R-3.in silico     301 CTTCGAAAAAAACAGTCGATCATTGCAATCAGATCAAAGAGGAGGTCGCGACGTATAATG 360


31287R-3.IR_full       361 AGCAGATATCCGCAAAAGTACCTTCCGTAAATTTGGTAACCAAGCTTATCGATTACCACA 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31287R-3.in silico     361 AGCAGATATCCGCAAAAGTACCTTCCGTAAATTTGGTAACCAAGCTTATCGATTACCACA 420


31287R-3.IR_full       421 ATAATACACTGGAGTCTATGGAAAAAAATATCGAAAAATTGAAGAGCCAGGTCACACAAG 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31287R-3.in silico     421 ATAATACACTGGAGTCTATGGAAAAAAATATCGAAAAATTGAAGAGCCAGGTCACACAAG 480


31287R-3.IR_full       481 TTCAAAAGGAGTTCGAGCAG 500
                           ||||||||||||||||||||
31287R-3.in silico     481 TTCAAAAGGAGTTCGAGCAG 500



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_169708.1  CG31287-RA (CG31287), mRNA 
0   0  3  3  2  NM_132868.3  CG8931-RA (CG8931), mRNA 
0   0  1  4  2  NM_136073.2  CG18397-RA (CG18397), mRNA 
0   0  1  3  5  NM_165961.1  CG3845-RA, transcript variant A (l(2)01424), mRNA 
0   0  1  3  5  NM_001038854.1  CG3845-RC, transcript variant C (l(2)01424), mRNA 
0   0  1  3  5  NM_143770.2  CG3845-RB, transcript variant B (l(2)01424), mRNA 
0   0  1  3  4  NM_176213.1  CG33197-RC, transcript variant C (mbl), mRNA 
0   0  1  3  2  NM_206442.1  CG1030-RC, transcript variant C (Scr), mRNA 
0   0  1  3  2  NM_206443.1  CG1030-RB, transcript variant B (Scr), mRNA 
0   0  1  3  2  NM_079524.3  CG1030-RA, transcript variant A (Scr), mRNA 
0   0  1  2  5  NM_143996.1  CG13643-RA (CG13643), mRNA 
0   0  0  6  3  NM_140953.2  CG5605-RA, transcript variant A (eRF1), mRNA 
0   0  0  6  3  NM_168848.1  CG5605-RF, transcript variant F (eRF1), mRNA 
0   0  0  6  3  NM_176369.1  CG5605-RG, transcript variant G (eRF1), mRNA 
0   0  0  6  3  NM_168847.1  CG5605-RE, transcript variant E (eRF1), mRNA 
0   0  0  6  3  NM_168845.1  CG5605-RB, transcript variant B (eRF1), mRNA 
0   0  0  6  3  NM_168846.1  CG5605-RC, transcript variant C (eRF1), mRNA 
0   0  0  5  5  NM_079009.2  CG18076-RA, transcript variant A (shot), mRNA 
0   0  0  5  5  NM_166017.1  CG18076-RE, transcript variant E (shot), mRNA 
0   0  0  5  5  NM_166016.1  CG18076-RB, transcript variant B (shot), mRNA 
0   0  0  5  5  NM_166015.1  CG18076-RG, transcript variant G (shot), mRNA 
0   0  0  5  5  NM_166018.1  CG18076-RC, transcript variant C (shot), mRNA 
0   0  0  5  5  NM_166019.1  CG18076-RH, transcript variant H (shot), mRNA 
0   0  0  5  3  NM_057214.3  CG4807-RA, transcript variant A (ab), mRNA 
0   0  0  5  3  NM_057215.3  CG4807-RB, transcript variant B (ab), mRNA 
0   0  0  4  6  NM_141376.2  CG15594-RA, transcript variant A (CG15594), mRNA 
0   0  0  4  4  NM_166218.1  CG15611-RB, transcript variant B (CG15611), mRNA 
0   0  0  4  4  NM_137345.2  CG15611-RA, transcript variant A (CG15611), mRNA 
0   0  0  4  3  NM_176181.1  CG8291-RC, transcript variant C (CG8291), mRNA 
0   0  0  4  3  NM_137220.2  CG8291-RA, transcript variant A (CG8291), mRNA 
 Wing Disc
GFP
 Puc
 Caspase
DAPI
 Merge
 Figure
JNK signal
N
 
DB
 
DC
+- 
DM
 
VM
 
VB
 
Caspase-3 signal
N
 
DB
 
DC
 
DM
 
VM
 
VB
 
Dcompartment   Penetrance
     

SHIGEN - SHared Information of GENetic resources - National Institute of Genetics (NIG)
 Copyright (c) National Institute of Genetics (NIG)
All rights reserved.
The NIG-Fly is a part of the National BioResource Project which started in 2002.