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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
31196R-3 |
| Symbol |
14-3-3epsilon |
Full Name |
14-3-3epsilon |
| CG No |
CG31196 |
Old CG No |
CG31196 |
| Synonyms |
PAR5, 14-3-3, par-5, Par-5, D14-3-3epsilon, D14-3-3e, 14-3-3-e, 14-3-3e, EK3-5, CT24092, 14-3-3epsilon, d14-3-3epsilon, SR3-9, l(3)j2B10, Su(Raf)3B, CG31196, anon-WO02059370.52, anon-WO0172774.141 |
| Accession No (Link to NCBI) |
NM_169796.1 |
| Inserted Chr. |
X |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Nam S, Le TP, Chung S, Choi KW.
Tctp regulates the level and localization of Foxo for cell growth in Drosophila.
Cell Death Discov (2022) 8(1) 146
[
PubMed ID = 35361773
]
[
RRC reference
]
Cho Y, Lai CM, Lin KY, Hsu HJ.
A Targeted RNAi Screen Reveals Drosophila Female-Sterile Genes That Control the Size of Germline Stem Cell Niche During Development.
G3 (Bethesda) (2018) 8(7) 2345-2354
[
PubMed ID = 29764959
]
[
RRC reference
]
Le TP, Vuong LT, Kim AR, Hsu YC, Choi KW.
14-3-3 proteins regulate Tctp-Rheb interaction for organ growth in Drosophila.
Nat Commun (2016) 7 11501
[
PubMed ID = 27151460
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgAATGGTGGAGGCCATGAAGAA |
| Primer Seq. 3' |
aatctagaggtaccCGGCGAGTTGAGAATCTCATA |
| Predicted Fragment Size |
499 |
| IR fragment full Seq |
0001 AATGGTGGAG GCCATGAAGA AGGTCGCCTC CATGGACGTA GAGCTGACCG TCGAGGAGCG
0061 AAATCTGCTG TCGGTGGCGT ACAAGAATGT GATTGGAGCA CGCCCGTCGC CTCGTTGGCG
0121 CATCATCACC TCGATCGAAC AGAAGGAGGA GAACAAGGGG GCCCGAGGAG AAATTGGAGA
0181 TGATCAAAAC CTACCGCGGA CAGGTGGAGA AGGAGCTGCG CGACATCTGC TCGGATATAC
0241 TGAACGTGCT CGAGAAGCAT CTCATTCCAT GCGCCACATC CGGCGAAAGC AAAGTATTCT
0301 ACTATAAGAT GAAGGGCGAC TACCATCGCT ACCTGGCCGA AtTNNNCACC GGCTCCGAcC
0361 GCAAGGATGC GGCAGAGAAC TCGCTGATTG CCTACAAGGC GGCCAGCGAT ATTGCCATGA
0421 ACGATCTGCC ACCAACACAC CCCATCCGTT TGGGCTTGGC ATTGAACTTC TCGGTGTTCT
0481 ACTATGAGAT TCTCAACTCG CCG |
| in silico PCR Fragment |
0001 AATGGTGGAG GCCATGAAGA AGGTCGCCTC CATGGACGTA GAGCTGACCG TCGAGGAGCG
0061 AAATCTGCTG TCGGTGGCGT ACAAGAATGT GATTGGAGCA CG-CCGT-GC CTCG-TGGCG
0121 CATCATCACC TCGATCGAAC AGAAGGAGGA GAACAAGGGG G-CCGAGGAG AAATTGGAGA
0181 TGATCAAAAC CTACCGCGGA CAGGTGGAGA AGGAGCTGCG CGACATCTGC TCGGATATAC
0241 TGAACGTGCT CGAGAAGCAT CTCATTCCAT GCGCCACATC CGGCGAAAGC AAAGTATTCT
0301 ACTATAAGAT GAAGGGCGAC TACCATCGCT ACCTGGCCGA ATTCGCCACC GGCTCCGACC
0361 GCAAGGATGC GGCAGAGAAC TCGCTGATTG CCTACAAGGC GGCCAGCGAT ATTGCCATGA
0421 ACGATCTGCC ACCAACACAC CCCATCCGTT TGGGCTTGGC ATTGAACTTC TCGGTGTTCT
0481 ACTATGAGAT TCTCAACTCG CCG |
| Assemble Data |
31196R-3.IR_full 1 AATGGTGGAGGCCATGAAGAAGGTCGCCTCCATGGACGTAGAGCTGACCGTCGAGGAGCG 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31196R-3.in silico 1 AATGGTGGAGGCCATGAAGAAGGTCGCCTCCATGGACGTAGAGCTGACCGTCGAGGAGCG 60
31196R-3.IR_full 61 AAATCTGCTGTCGGTGGCGTACAAGAATGTGATTGGAGCACGCCCGTCGCCTCGTTGGCG 120
|||||||||||||||||||||||||||||||||||||||||| |||| |||||| |||||
31196R-3.in silico 61 AAATCTGCTGTCGGTGGCGTACAAGAATGTGATTGGAGCACG-CCGT-GCCTCG-TGGCG 120
31196R-3.IR_full 121 CATCATCACCTCGATCGAACAGAAGGAGGAGAACAAGGGGGCCCGAGGAGAAATTGGAGA 180
||||||||||||||||||||||||||||||||||||||||| ||||||||||||||||||
31196R-3.in silico 121 CATCATCACCTCGATCGAACAGAAGGAGGAGAACAAGGGGG-CCGAGGAGAAATTGGAGA 180
31196R-3.IR_full 181 TGATCAAAACCTACCGCGGACAGGTGGAGAAGGAGCTGCGCGACATCTGCTCGGATATAC 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31196R-3.in silico 181 TGATCAAAACCTACCGCGGACAGGTGGAGAAGGAGCTGCGCGACATCTGCTCGGATATAC 240
31196R-3.IR_full 241 TGAACGTGCTCGAGAAGCATCTCATTCCATGCGCCACATCCGGCGAAAGCAAAGTATTCT 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31196R-3.in silico 241 TGAACGTGCTCGAGAAGCATCTCATTCCATGCGCCACATCCGGCGAAAGCAAAGTATTCT 300
31196R-3.IR_full 301 ACTATAAGATGAAGGGCGACTACCATCGCTACCTGGCCGAATTNNNCACCGGCTCCGACC 360
||||||||||||||||||||||||||||||||||||||||||| ||||||||||||||
31196R-3.in silico 301 ACTATAAGATGAAGGGCGACTACCATCGCTACCTGGCCGAATTCGCCACCGGCTCCGACC 360
31196R-3.IR_full 361 GCAAGGATGCGGCAGAGAACTCGCTGATTGCCTACAAGGCGGCCAGCGATATTGCCATGA 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31196R-3.in silico 361 GCAAGGATGCGGCAGAGAACTCGCTGATTGCCTACAAGGCGGCCAGCGATATTGCCATGA 420
31196R-3.IR_full 421 ACGATCTGCCACCAACACACCCCATCCGTTTGGGCTTGGCATTGAACTTCTCGGTGTTCT 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31196R-3.in silico 421 ACGATCTGCCACCAACACACCCCATCCGTTTGGGCTTGGCATTGAACTTCTCGGTGTTCT 480
31196R-3.IR_full 481 ACTATGAGATTCTCAACTCGCCG 503
|||||||||||||||||||||||
31196R-3.in silico 481 ACTATGAGATTCTCAACTCGCCG 503
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
481 |
0 |
0 |
0 |
NM_169796.1 |
CG31196-RA, transcript variant A (14-3-3epsilon), mRNA |
| 100 |
481 |
0 |
0 |
0 |
NM_169797.1 |
CG31196-RB, transcript variant B (14-3-3epsilon), mRNA |
| 100 |
481 |
0 |
0 |
0 |
NM_169798.1 |
CG31196-RD, transcript variant D (14-3-3epsilon), mRNA |
| 100 |
481 |
0 |
0 |
0 |
NM_169799.1 |
CG31196-RC, transcript variant C (14-3-3epsilon), mRNA |
| 0 |
0 |
6 |
2 |
6 |
NM_140068.2 |
CG3428-RA (CG3428), mRNA |
| 0 |
0 |
5 |
22 |
36 |
NM_001014516.1 |
CG17870-RI, transcript variant I (14-3-3zeta), mRNA |
| 0 |
0 |
5 |
22 |
36 |
NM_165741.2 |
CG17870-RA, transcript variant A (14-3-3zeta), mRNA |
| 0 |
0 |
5 |
22 |
36 |
NM_001014515.1 |
CG17870-RJ, transcript variant J (14-3-3zeta), mRNA |
| 0 |
0 |
5 |
22 |
36 |
NM_057537.3 |
CG17870-RD, transcript variant D (14-3-3zeta), mRNA |
| 0 |
0 |
5 |
22 |
36 |
NM_165745.2 |
CG17870-RG, transcript variant G (14-3-3zeta), mRNA |
| 0 |
0 |
5 |
22 |
36 |
NM_206070.1 |
CG17870-RH, transcript variant H (14-3-3zeta), mRNA |
| 0 |
0 |
5 |
22 |
36 |
NM_165740.2 |
CG17870-RE, transcript variant E (14-3-3zeta), mRNA |
| 0 |
0 |
5 |
22 |
36 |
NM_165742.1 |
CG17870-RB, transcript variant B (14-3-3zeta), mRNA |
| 0 |
0 |
3 |
6 |
6 |
NM_205934.1 |
CG13391-RB, transcript variant B (Aats-ala), mRNA |
| 0 |
0 |
3 |
6 |
6 |
NM_078787.2 |
CG13391-RA, transcript variant A (Aats-ala), mRNA |
| 0 |
0 |
3 |
3 |
8 |
NM_142668.2 |
CG3822-RA (CG3822), mRNA |
| 0 |
0 |
2 |
7 |
5 |
NM_078867.2 |
CG5526-RA (Dhc36C), mRNA |
| 0 |
0 |
2 |
6 |
5 |
NM_167364.1 |
CG15747-RA (CG15747), mRNA |
| 0 |
0 |
2 |
2 |
21 |
NM_166125.2 |
CG18255-RA, transcript variant A (Strn-Mlck), mRNA |
| 0 |
0 |
2 |
2 |
13 |
NM_166129.2 |
CG18255-RD, transcript variant D (Strn-Mlck), mRNA |
| 0 |
0 |
2 |
2 |
6 |
NM_206012.1 |
CG33322-RA (CG33322), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_140400.1 |
CG10083-RA (CG10083), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_134486.1 |
CG14221-RA (CG14221), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_080021.2 |
CG2952-RA (Dox-A3), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_058091.3 |
CG3152-RA (Trap1), mRNA |
| 0 |
0 |
1 |
7 |
2 |
NM_001038808.1 |
CG17140-RA, transcript variant A (CG17140), mRNA |
| 0 |
0 |
1 |
7 |
2 |
NM_001038809.1 |
CG17139-RA, transcript variant A (CG17139), mRNA |
| 0 |
0 |
1 |
5 |
14 |
NM_001043251.1 |
CG31045-RF, transcript variant F (Mhcl), mRNA |
| 0 |
0 |
1 |
5 |
14 |
NM_169701.2 |
CG31045-RB, transcript variant B (Mhcl), mRNA |
| 0 |
0 |
1 |
5 |
14 |
NM_169700.2 |
CG31045-RA, transcript variant A (Mhcl), mRNA |
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