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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 3117R-1 
 Symbol CG3117  Full Name CG3117 
 CG No CG3117  Old CG No CG3117 
 Synonyms SPH94, c-SPH94, CG3117 
 Accession No (Link to NCBI) NM_134878.1 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]

Kambris Z, Brun S, Jang IH, Nam HJ, Romeo Y, Takahashi K, Lee WJ, Ueda R, Lemaitre B.
Drosophila immunity: a large-scale in vivo RNAi screen identifies five serine proteases required for Toll activation.
Curr Biol (2006) 16(8) 808-13 [ PubMed ID = 16631589 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgTTGGGAGAAGATGTCGCGGTT 
 Primer Seq. 3' 
aatctagaggtaccGACGATCCATCGGTGGATTTA 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 TTGGGAGAAG ATGTCGCGGT TGTGCACTTC TTTGGTCATC ATTTTGGGTC TCATCCTCTC 
0061 GAGCGGTGGC ACTCACATAT GCCTGAGCTC CAATCTGTGT GTTCCCAGGG AAAATTGTCG 
0121 TGAAGAGATG CCGTTCTTCG ACTTTAGTTC CACCATTGAG TGCTCCGACG AAGAAGTGTG 
0181 CTGTGAAAAG TCAAATGTGA TAGGCATGAG TAAGAGTCCT CCACAACATT CAGTGGACAC 
0241 ACTTCTTAGG ACAAGTTATC CAAATGCCCT GGATGGAAGC CCTCAGGTGT TTGGTGACCA 
0301 AACCAAACCG AATCAATTTC CCTGGGTGAC GGCTTTGTTC GCCAAGGGAT CTTATCTGGG 
0361 AGGAGGATCT CTGATTACCC CAGGACTTGT ACTAACCGCT GCCCACATCC TAGCGGGATT 
0421 ATCCCCCAAT GACATCATGG TTAGAGCTGG CGAATGGGAT CTATCCTCCA GTGAGAAACT 
0481 AAATCCACCG ATGGATCGTC  
 in silico PCR Fragment 
0001 TTGGGAGAAG ATGTCGCGGT TGTGCACTTC TTTGGTCATC ATTTTGGGTC TCATCCTCTC 
0061 GAGCGGTGGC ACTCACATAT GCCTGAGCTC CAATCTGTGT GTTCCCAGGG AAAATTGTCG 
0121 TGAAGAGATG CCGTTCTTCG ACTTTAGTTC CACCATTGAG TGCTCCGACG AAGAAGTGTG 
0181 CTGTGAAAAG TCAAATGTGA TAGGCATGAG TAAGAGTCCT CCACAACATT CAGTGGACAC 
0241 ACTTCTTAGG ACAAGTTATC CAAATGCCCT GGATGGAAGC CCTCAGGTGT TTGGTGACCA 
0301 AACCAAACCG AATCAATTTC CCTGGGTGAC GGCTTTGTTC GCCAAGGGAT CTTATCTGGG 
0361 AGGAGGATCT CTGATTACCC CAGGACTTGT ACTAACCGCT GCCCACATCC TAGCGGGATT 
0421 ATCCCCCAAT GACATCATGG TTAGAGCTGG CGAATGGGAT CTATCCTCCA GTGAGAAACT 
0481 AAATCCACCG ATGGATCGTC  
 Assemble Data 

3117R-1.IR_full       1   TTGGGAGAAGATGTCGCGGTTGTGCACTTCTTTGGTCATCATTTTGGGTCTCATCCTCTC 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3117R-1.in silico     1   TTGGGAGAAGATGTCGCGGTTGTGCACTTCTTTGGTCATCATTTTGGGTCTCATCCTCTC 60


3117R-1.IR_full       61  GAGCGGTGGCACTCACATATGCCTGAGCTCCAATCTGTGTGTTCCCAGGGAAAATTGTCG 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3117R-1.in silico     61  GAGCGGTGGCACTCACATATGCCTGAGCTCCAATCTGTGTGTTCCCAGGGAAAATTGTCG 120


3117R-1.IR_full       121 TGAAGAGATGCCGTTCTTCGACTTTAGTTCCACCATTGAGTGCTCCGACGAAGAAGTGTG 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3117R-1.in silico     121 TGAAGAGATGCCGTTCTTCGACTTTAGTTCCACCATTGAGTGCTCCGACGAAGAAGTGTG 180


3117R-1.IR_full       181 CTGTGAAAAGTCAAATGTGATAGGCATGAGTAAGAGTCCTCCACAACATTCAGTGGACAC 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3117R-1.in silico     181 CTGTGAAAAGTCAAATGTGATAGGCATGAGTAAGAGTCCTCCACAACATTCAGTGGACAC 240


3117R-1.IR_full       241 ACTTCTTAGGACAAGTTATCCAAATGCCCTGGATGGAAGCCCTCAGGTGTTTGGTGACCA 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3117R-1.in silico     241 ACTTCTTAGGACAAGTTATCCAAATGCCCTGGATGGAAGCCCTCAGGTGTTTGGTGACCA 300


3117R-1.IR_full       301 AACCAAACCGAATCAATTTCCCTGGGTGACGGCTTTGTTCGCCAAGGGATCTTATCTGGG 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3117R-1.in silico     301 AACCAAACCGAATCAATTTCCCTGGGTGACGGCTTTGTTCGCCAAGGGATCTTATCTGGG 360


3117R-1.IR_full       361 AGGAGGATCTCTGATTACCCCAGGACTTGTACTAACCGCTGCCCACATCCTAGCGGGATT 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3117R-1.in silico     361 AGGAGGATCTCTGATTACCCCAGGACTTGTACTAACCGCTGCCCACATCCTAGCGGGATT 420


3117R-1.IR_full       421 ATCCCCCAATGACATCATGGTTAGAGCTGGCGAATGGGATCTATCCTCCAGTGAGAAACT 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3117R-1.in silico     421 ATCCCCCAATGACATCATGGTTAGAGCTGGCGAATGGGATCTATCCTCCAGTGAGAAACT 480


3117R-1.IR_full       481 AAATCCACCGATGGATCGTC 500
                          ||||||||||||||||||||
3117R-1.in silico     481 AAATCCACCGATGGATCGTC 500



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_134878.1  CG3117-RA (CG3117), mRNA 
0   0  4  3  2  NM_135530.2  CG5390-RA (CG5390), mRNA 
0   0  2  6  9  NM_132725.2  CG1461-RA (CG1461), mRNA 
0   0  2  3  2  NM_137689.2  CG3221-RA (CG3221), mRNA 
0   0  2  2  5  NM_136558.2  CG8586-RA (CG8586), mRNA 
0   0  1  4  5  NM_057943.3  CG12249-RA, transcript variant A (mira), mRNA 
0   0  1  4  5  NM_057944.3  CG12249-RB, transcript variant B (mira), mRNA 
0   0  1  3  2  NM_001038781.1  CG33979-RB, transcript variant B (capt), mRNA 
0   0  1  3  2  NM_132755.1  CG9509-RA (CG9509), mRNA 
0   0  1  2  3  NM_166171.2  CG30098-RA (CG30098), mRNA 
0   0  1  2  3  NM_078701.2  CG32508-RC, transcript variant C (shakB), mRNA 
0   0  1  2  2  NM_142933.1  CG13597-RA (CG13597), mRNA 
0   0  1  2  2  NM_168540.1  CG11282-RB, transcript variant B (caps), mRNA 
0   0  1  2  2  NM_079332.2  CG11282-RA, transcript variant A (caps), mRNA 
0   0  1  2  2  NM_132831.1  CG8184-RB (CG8184), mRNA 
0   0  1  2  2  NM_001043139.1  CG11282-RC, transcript variant C (caps), mRNA 
0   0  0  8  9  NM_134801.1  CG4259-RA (CG4259), mRNA 
0   0  0  7  6  NM_168379.1  CG32050-RA (CG32050), mRNA 
0   0  0  5  5  NM_134766.3  CG31666-RA, transcript variant A (CG31666), mRNA 
0   0  0  5  5  NM_164428.2  CG31666-RB, transcript variant B (CG31666), mRNA 
0   0  0  5  5  NM_164430.2  CG31666-RD, transcript variant D (CG31666), mRNA 
0   0  0  5  5  NM_164429.1  CG31666-RC, transcript variant C (CG31666), mRNA 
0   0  0  5  3  NM_135980.2  CG4841-RA (CG4841), mRNA 
0   0  0  4  4  NM_130666.2  CG2681-RA (CG2681), mRNA 
0   0  0  4  3  NM_168208.1  CG8588-RC, transcript variant C (pst), mRNA 
0   0  0  4  3  NM_168209.1  CG8588-RD, transcript variant D (pst), mRNA 
0   0  0  4  3  NM_139853.1  CG8588-RA, transcript variant A (pst), mRNA 
0   0  0  4  3  NM_132487.1  CG15196-RA (CG15196), mRNA 
0   0  0  4  3  NM_168207.1  CG8588-RB, transcript variant B (pst), mRNA 
0   0  0  4  3  NM_169775.1  CG7467-RA, transcript variant A (osa), mRNA 

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