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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 31051R-3

Symbol CG31051 Full Name CG31051

CG No CG31051 Old CG No CG31051

Synonyms CT3186, CG1540, CG31051

Accession No (Link to NCBI) NM_170368.1

Inserted Chr. X

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees pupal semi-lethal

Map Viewer

Reference
[Please submit your publication] Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624 [ PubMed ID = 21666802 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgTCCAACATCTACGAGGATGGG

Primer Seq. 3'

aatctagaggtaccTCCGACTCCAGATCCGAGTCA

Predicted Fragment Size

IR fragment full Seq

0001 TCCAACATCT ACGAGGATGG GGCTGCGGGA CTCTTGAAAA CGGCCAGCGG AGCACCCAAA 
0061 CATATGTCGG ATCTGGAGTT TGAGGTGGCA TTCAGGGGTG CCGGCAAGGT CTATGCCCCC 
0121 ACGACCATAG AAATCGATGA AGAGGAGGAA GCGACCGCAG GAAGCGGTCG AGGCCACTGA 
0181 GCCACCCAAC ACCTGCCGCA TACTGCAGCG CAAACTGGAG CTGAAGGTCG AGCGGGCCCG 
0241 GCNGGAACTA CACTCAGTTT CAGGAGGAGC AGGCTACCAA GACGCAGTCG TCCACTCTGA 
0301 TACCAATCAA CCGTCTACCC ATTCCTGGCG AGCCGGAACA CAAGCCACTG GTGGACTACA 
0361 AATCCGAGAG TAGCGACGAG GCCGAGGAGA TCTCTTTCTT TCCGGCCCAG CTGAAGCGGT 
0421 CCAAGCGCCG CCAGCAGAAC CACGAACTGA CTGACACCTT CAGCATACAG GAGATGACCA 
0481 TTGACTCGGA TCTGGAGTCG GA

in silico PCR Fragment

0001 TCCAACATCT ACGAGGATGG GGCTGCGGGA CTCTTGAAAA CGGCCAGCGG AGCACCCAAA 
0061 CATATGTCAG ATCTGGAGTT TGAGGTGGCA TTCAGGGGTG CCGGCAAGGT CTATGCCCCC 
0121 ACGACCATAG AAATCGATGA AGAGGAGGAA GCGACCGCAG GAAGCGGTCG AGGCCAC-GA 
0181 GCCACCCAAC ACCTGCCGCA TACTGCAGCG CAAACTGGAG CTGAAGGTCG AGCGGGCCCG 
0241 GC-GGAACTA CACTCAGTTT CAGGCGGAGC AGGCTACCAA GACGCAGTCG TCCACTCTGA 
0301 TACCAATCAA CCGTCTACCC ATTCCTGGCG AGCCGGAACA CAAGCCACTG GTGGACTACA 
0361 AATCCGAGAG TAGCGACGAG GCCGAGGAGA TCTCCTTCTT TCCGGCCCAG CTGAAGCGGT 
0421 CCAAGCGCCG CCAGCAGAAC CACGAACTGA CTGACACCTT CAGCATACAG GAGATGACCA 
0481 TTGACTCGGA TCTGGAGTCG GA

Assemble Data


31051R-3.IR_full       1   TCCAACATCTACGAGGATGGGGCTGCGGGACTCTTGAAAACGGCCAGCGGAGCACCCAAA 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31051R-3.in silico     1   TCCAACATCTACGAGGATGGGGCTGCGGGACTCTTGAAAACGGCCAGCGGAGCACCCAAA 60


31051R-3.IR_full       61  CATATGTCGGATCTGGAGTTTGAGGTGGCATTCAGGGGTGCCGGCAAGGTCTATGCCCCC 120
                           |||||||| |||||||||||||||||||||||||||||||||||||||||||||||||||
31051R-3.in silico     61  CATATGTCAGATCTGGAGTTTGAGGTGGCATTCAGGGGTGCCGGCAAGGTCTATGCCCCC 120


31051R-3.IR_full       121 ACGACCATAGAAATCGATGAAGAGGAGGAAGCGACCGCAGGAAGCGGTCGAGGCCACTGA 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||| ||
31051R-3.in silico     121 ACGACCATAGAAATCGATGAAGAGGAGGAAGCGACCGCAGGAAGCGGTCGAGGCCAC-GA 180


31051R-3.IR_full       181 GCCACCCAACACCTGCCGCATACTGCAGCGCAAACTGGAGCTGAAGGTCGAGCGGGCCCG 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31051R-3.in silico     181 GCCACCCAACACCTGCCGCATACTGCAGCGCAAACTGGAGCTGAAGGTCGAGCGGGCCCG 240


31051R-3.IR_full       241 GCNGGAACTACACTCAGTTTCAGGAGGAGCAGGCTACCAAGACGCAGTCGTCCACTCTGA 300
                           || ||||||||||||||||||||| |||||||||||||||||||||||||||||||||||
31051R-3.in silico     241 GC-GGAACTACACTCAGTTTCAGGCGGAGCAGGCTACCAAGACGCAGTCGTCCACTCTGA 300


31051R-3.IR_full       301 TACCAATCAACCGTCTACCCATTCCTGGCGAGCCGGAACACAAGCCACTGGTGGACTACA 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31051R-3.in silico     301 TACCAATCAACCGTCTACCCATTCCTGGCGAGCCGGAACACAAGCCACTGGTGGACTACA 360


31051R-3.IR_full       361 AATCCGAGAGTAGCGACGAGGCCGAGGAGATCTCTTTCTTTCCGGCCCAGCTGAAGCGGT 420
                           |||||||||||||||||||||||||||||||||| |||||||||||||||||||||||||
31051R-3.in silico     361 AATCCGAGAGTAGCGACGAGGCCGAGGAGATCTCCTTCTTTCCGGCCCAGCTGAAGCGGT 420


31051R-3.IR_full       421 CCAAGCGCCGCCAGCAGAACCACGAACTGACTGACACCTTCAGCATACAGGAGATGACCA 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31051R-3.in silico     421 CCAAGCGCCGCCAGCAGAACCACGAACTGACTGACACCTTCAGCATACAGGAGATGACCA 480


31051R-3.IR_full       481 TTGACTCGGATCTGGAGTCGGA 502
                           ||||||||||||||||||||||
31051R-3.in silico     481 TTGACTCGGATCTGGAGTCGGA 502

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_170368.1	CG31051-RA (CG31051), mRNA
0.2	1	3	2	2	NM_135093.2	CG14015-RA (CG14015), mRNA
0	0	5	5	4	NM_132329.3	CG16892-RA (CG16892), mRNA
0	0	4	2	3	NM_140776.2	CG13699-RA (CG13699), mRNA
0	0	4	2	3	NM_142457.2	CG7669-RA (CG7669), mRNA
0	0	2	3	4	NM_132200.1	CG1531-RB (CG1531), mRNA
0	0	2	2	3	NM_168147.1	CG32407-RA (CG32407), mRNA
0	0	2	2	2	NM_080079.2	CG32120-RA (sens), mRNA
0	0	2	2	1	NM_176001.1	CG32982-RA, transcript variant A (CG32982), mRNA
0	0	2	2	1	NM_001042884.1	CG32982-RC, transcript variant C (CG32982), mRNA
0	0	2	2	1	NM_176002.1	CG32982-RB, transcript variant B (CG32982), mRNA
0	0	1	7	4	NM_137205.2	CG8195-RA (CG8195), mRNA
0	0	1	5	15	NM_166125.2	CG18255-RA, transcript variant A (Strn-Mlck), mRNA
0	0	1	5	8	NM_166129.2	CG18255-RD, transcript variant D (Strn-Mlck), mRNA
0	0	1	4	6	NM_057245.2	CG6189-RA (l(1)1Bi), mRNA
0	0	1	4	3	NM_139998.2	CG5804-RA (CG5804), mRNA
0	0	1	4	3	NM_135806.1	CG16957-RA (CG16957), mRNA
0	0	1	3	7	NM_135461.2	CG4364-RA (CG4364), mRNA
0	0	1	3	6	NM_165511.2	CG30385-RA (CG30385), mRNA
0	0	1	3	5	NM_132432.1	CG2186-RA (CG2186), mRNA
0	0	1	3	3	NM_134669.2	CG11601-RA (CG11601), mRNA
0	0	1	3	3	NM_142335.2	CG5208-RA (CG5208), mRNA
0	0	1	3	2	NM_130628.2	CG3621-RA (CG3621), mRNA
0	0	1	2	7	NM_170094.2	CG13598-RA, transcript variant A (sba), mRNA
0	0	1	2	6	NM_176549.1	CG13598-RB, transcript variant B (sba), mRNA
0	0	1	2	4	NM_140569.2	CG5841-RA (mib1), mRNA
0	0	1	2	3	NM_176299.1	CG33057-RA, transcript variant A (CG33057), mRNA
0	0	1	2	3	NM_136419.2	CG30384-RA (CG30384), mRNA
0	0	1	2	3	NM_168272.1	CG7163-RA, transcript variant A (mkg-p), mRNA
0	0	1	2	3	NM_170528.2	CG31012-RD, transcript variant D (CG31012), mRNA

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