NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 31044R-1

Symbol CG31044 Full Name CG31044

CG No CG31044 Old CG No CG31044

Synonyms BcDNA:SD09455, CG31044

Accession No (Link to NCBI) NM_170400.1

Inserted Chr. ll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
[Please submit your publication] Ohhara Y, Nakamura A, Kato Y, Yamakawa-Kobayashi K.
Chaperonin TRiC/CCT supports mitotic exit and entry into endocycle in Drosophila.
PLoS Genet (2019) 15(4) e1008121 [ PubMed ID = 31034473 ] [ RRC reference ]

Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]

Molnar C, Casado M, López-Varea A, Cruz C, de Celis JF.
Genetic annotation of gain-of-function screens using RNA interference and in situ hybridization of candidate genes in the Drosophila wing.
Genetics (2012) 192(2) 741-52 [ PubMed ID = 22798488 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGCCGGAACACCTTTCAACCCC

Primer Seq. 3'

aatctagaggtaccTAGTTGCGGCGTTTGACGGCT

Predicted Fragment Size

IR fragment full Seq

0001 GCCGGAACAC CTTTCAACCC CGTGGGCCAT TGTTAGTGCT CCGTGGCTAG GGATAGATGG 
0061 CTACTGTATG GCCACTTGCT GCACGGCCAT GAAGCTACTC CTCCCACACG ATTTGCTTCA 
0121 CGCTCATTTA AATGCCAACG CACCGGAGGG AGGAGAGAGC AGGGCACTTC ACTTCGGCGG 
0181 CTCGGCTGGC TTATTTACAT ATTTTCTAAA TGAATATGGG CCCCGCGAGT TTGCAACCCC 
0241 GACCGGCGGT GGCAAAGCCG AGAAGCGCAT TCAGTTGCAA CGGCCGCGGC AGCTGGCTTT 
0301 CCCTGCACTG GATTTCCACT TCCTCCTGGT CGAAGCAGCC GCAGCAGCAG CAGCAATAAC 
0361 AATCAGGAAC AGCAGCAGCA GCAGCAACAA GAAGCCGTCA AACGCCGCAA CTA

in silico PCR Fragment

0001 GCCGGAACAC CTTTCAACCC CGTGGGCCAT TGTTAGTGCT CCGTGGCTAG GGATAGATGG 
0061 CTACTGTATG GCCACTTGCT GCACGGCCAT GAAGCTACTC CTCCCACACG ATTTGCTTCA 
0121 CGCTCATTTA AATGCCAACG CACCGCAGGG AGGAGAGAGC AGGGCACTTC ACTTCGGCGG 
0181 CTCGGCTGGC TTATTTACAT ATTTTCTAAA TGAATATGGG CCCCGCGAGT TTGCAACCCC 
0241 GACCGGCGGT GGCAAAGCCG AGAAGCGCAT TCAGTTGCAA CGGCCGCGGC AGCTGGCTTT 
0301 CCCTGCACTG GATTTCCACT TCCTCCTGGT CGAAGCAGCC GCAGCAGCAG CAGCAATAAC 
0361 AATCAGGAAC AGCAGCAGCA GCAGCAACAA GAAGCCGTCA AACGCCGCAA CTA

Assemble Data


31044R-1.IR_full       1   GCCGGAACACCTTTCAACCCCGTGGGCCATTGTTAGTGCTCCGTGGCTAGGGATAGATGG 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31044R-1.in silico     1   GCCGGAACACCTTTCAACCCCGTGGGCCATTGTTAGTGCTCCGTGGCTAGGGATAGATGG 60


31044R-1.IR_full       61  CTACTGTATGGCCACTTGCTGCACGGCCATGAAGCTACTCCTCCCACACGATTTGCTTCA 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31044R-1.in silico     61  CTACTGTATGGCCACTTGCTGCACGGCCATGAAGCTACTCCTCCCACACGATTTGCTTCA 120


31044R-1.IR_full       121 CGCTCATTTAAATGCCAACGCACCGGAGGGAGGAGAGAGCAGGGCACTTCACTTCGGCGG 180
                           ||||||||||||||||||||||||| ||||||||||||||||||||||||||||||||||
31044R-1.in silico     121 CGCTCATTTAAATGCCAACGCACCGCAGGGAGGAGAGAGCAGGGCACTTCACTTCGGCGG 180


31044R-1.IR_full       181 CTCGGCTGGCTTATTTACATATTTTCTAAATGAATATGGGCCCCGCGAGTTTGCAACCCC 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31044R-1.in silico     181 CTCGGCTGGCTTATTTACATATTTTCTAAATGAATATGGGCCCCGCGAGTTTGCAACCCC 240


31044R-1.IR_full       241 GACCGGCGGTGGCAAAGCCGAGAAGCGCATTCAGTTGCAACGGCCGCGGCAGCTGGCTTT 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31044R-1.in silico     241 GACCGGCGGTGGCAAAGCCGAGAAGCGCATTCAGTTGCAACGGCCGCGGCAGCTGGCTTT 300


31044R-1.IR_full       301 CCCTGCACTGGATTTCCACTTCCTCCTGGTCGAAGCAGCCGCAGCAGCAGCAGCAATAAC 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31044R-1.in silico     301 CCCTGCACTGGATTTCCACTTCCTCCTGGTCGAAGCAGCCGCAGCAGCAGCAGCAATAAC 360


31044R-1.IR_full       361 AATCAGGAACAGCAGCAGCAGCAGCAACAAGAAGCCGTCAAACGCCGCAACTA 413
                           |||||||||||||||||||||||||||||||||||||||||||||||||||||
31044R-1.in silico     361 AATCAGGAACAGCAGCAGCAGCAGCAACAAGAAGCCGTCAAACGCCGCAACTA 413

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	395	2	46	63	NM_170400.1	CG31044-RA (CG31044), mRNA
11.89	47	418	1126	2192	NM_080536.2	CG4013-RA, transcript variant A (Smr), mRNA
11.89	47	418	1126	2192	NM_167334.1	CG4013-RB, transcript variant B (Smr), mRNA
11.89	47	418	1126	2192	NM_167335.1	CG4013-RC, transcript variant C (Smr), mRNA
5.06	20	122	369	724	NM_168571.2	CG32133-RA (CG32133), mRNA
4.3	17	161	465	1049	NM_166030.1	CG8118-RB, transcript variant B (mam), mRNA
4.3	17	161	465	1049	NM_080376.1	CG8118-RA, transcript variant A (mam), mRNA
3.79	15	55	222	457	NM_057497.3	CG5058-RD, transcript variant D (grh), mRNA
3.79	15	55	222	457	NM_057496.3	CG5058-RC, transcript variant C (grh), mRNA
3.29	13	106	248	483	NM_134474.4	CG32532-RA (CG32532), mRNA
3.29	13	66	312	676	NM_079903.2	CG15319-RB (nej), mRNA
3.29	13	63	246	396	NM_167000.1	CG32778-RA (CG32778), mRNA
3.03	12	100	233	526	NM_132246.2	CG10555-RA (CG10555), mRNA
3.03	12	81	392	873	NM_001038734.1	CG16902-RC (Hr4), mRNA
3.03	12	62	302	560	NM_080364.3	CG5461-RA, transcript variant A (bun), mRNA
3.03	12	62	292	519	NM_001042894.1	CG5461-RF, transcript variant F (bun), mRNA
3.03	12	59	218	425	NM_078514.2	CG9653-RA (brk), mRNA
3.03	12	49	187	364	NM_078575.2	CG9355-RA (dy), mRNA
3.03	12	45	180	310	NM_143700.3	CG17724-RA, transcript variant A (CG17724), mRNA
3.03	12	34	143	350	NM_001032244.1	CG32904-RA, transcript variant A (seq), mRNA
2.78	11	70	264	723	NM_169783.1	CG31243-RB, transcript variant B (cpo), mRNA
2.78	11	70	263	714	NM_169781.1	CG31243-RE, transcript variant E (cpo), mRNA
2.78	11	70	263	714	NM_169782.1	CG31243-RA, transcript variant A (cpo), mRNA
2.78	11	56	292	652	NM_139493.2	CG2083-RA (CG2083), mRNA
2.78	11	50	169	309	NM_057495.3	CG5058-RB, transcript variant B (grh), mRNA
2.78	11	50	169	309	NM_057494.3	CG5058-RA, transcript variant A (grh), mRNA
2.78	11	49	234	548	NM_176556.1	CG33106-RA, transcript variant A (mask), mRNA
2.78	11	49	234	547	NM_176557.1	CG33106-RB, transcript variant B (mask), mRNA
2.78	11	49	208	501	NM_137690.2	CG10543-RA, transcript variant A (CG10543), mRNA
2.78	11	41	183	442	NM_166416.1	CG10543-RB, transcript variant B (CG10543), mRNA

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