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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 31003R-1 
 Symbol gskt  Full Name gasket 
 CG No CG31003  Old CG No CG31003 
 Synonyms CG31003, NGSK, GSK3b, CT4237, CG11338, mjl, BEST:GH16447, NEST:bs22e06, Gsk3b, BcDNA:AT21229, gskt 
 Accession No (Link to NCBI) NM_170547.2 
 Inserted Chr. X 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgGTGGGTTGACCAACAAGGTGA 
 Primer Seq. 3' 
aatctagaggtaccCTTAAGCACACCCGTCTCCGA 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 GTGGGTTGAC CAACAAGGTG ACCACGGTGG TGGCCANCAA TGCCTTCGGC GCTGATGTGA 
0061 TGAGCGAGAT CAGCTACACG GACGCGAAAG TTGTGGGCAA TGGCAGCTTC GGCGTTGGTC 
0121 TTCCAGGCCA AAATGGTGCC CAGCAACGAA ATGGTGGCCA TAAAGAAGGT CCTGCAGGAT 
0181 CGGCGCTTCA AGAATCGGGA ACTGCAAATC ATGCGCAAGC TGCGCCACGA TAACATCATC 
0241 ACTCTCAAGT GGTTCTTCTT TTCAAGTGGC GAGAAAAGGG ACGAGGTCTA TCTGAACCTG 
0301 GTCATGGAGT TCTTGCCGGA GACGCTGTAT AAAGTGGAGC GACAGTACGC AAGGGCAAAG 
0361 CAAACCTTGC CAGTTAACTT TGTCCGACTT TACATGTACC AGCTGCTGAG GAGCATGGGA 
0421 TATCTCCACA GCTTGGGATT CTGTCACAGG GACATCAAGC CGCAGAACAT GCTGCTCGAT 
0481 TCGGAGACGG GTGTGCTTAA G 
 in silico PCR Fragment 
0001 GTGGGTTGAC CAACAAGGTG ACCACGGTGG TGGCCACCAA TGCCTTCGGC GCTGATGTGA 
0061 TGAGCGAGAT CAGCTACACG GACGCGAAAG TTGTGGGCAA TGGCAGCTTC GGCG-TGGTC 
0121 TTCCAGGCCA AAATGGTGCC CAGCAACGAA ATGGTGGCCA TAAAGAAGGT CCTGCAGGAT 
0181 CGGCGCTTCA AGAATCGGGA ACTGCAAATC ATGCGCAAGC TGCGCCACGA TAACATCATA 
0241 ACTCTCAAGT GGTTCTTCTT TTCAAGTGGC GAGAAAAGGG ACGAGGTCTA TCTGAACCTG 
0301 GTCATGGAGT TCTTGCCGGA GACGCTGTAT AAAGTGGAGC GACAGTACGC AAGGGCAAAG 
0361 CAAACCTTGC CAGTTAACTT TGTCCGACTT TACATGTACC AGCTGCTGAG GAGCATGGGA 
0421 TATCTCCACA GCTTGGGATT CTGTCACAGG GACATCAAGC CGCAGAACAT GCTGCTCGAT 
0481 TCGGAGACGG GTGTGCTTAA G 
 Assemble Data 

31003R-1.IR_full       1   GTGGGTTGACCAACAAGGTGACCACGGTGGTGGCCANCAATGCCTTCGGCGCTGATGTGA 60
                           |||||||||||||||||||||||||||||||||||| |||||||||||||||||||||||
31003R-1.in silico     1   GTGGGTTGACCAACAAGGTGACCACGGTGGTGGCCACCAATGCCTTCGGCGCTGATGTGA 60


31003R-1.IR_full       61  TGAGCGAGATCAGCTACACGGACGCGAAAGTTGTGGGCAATGGCAGCTTCGGCGTTGGTC 120
                           |||||||||||||||||||||||||||||||||||||||||||||||||||||| |||||
31003R-1.in silico     61  TGAGCGAGATCAGCTACACGGACGCGAAAGTTGTGGGCAATGGCAGCTTCGGCG-TGGTC 120


31003R-1.IR_full       121 TTCCAGGCCAAAATGGTGCCCAGCAACGAAATGGTGGCCATAAAGAAGGTCCTGCAGGAT 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31003R-1.in silico     121 TTCCAGGCCAAAATGGTGCCCAGCAACGAAATGGTGGCCATAAAGAAGGTCCTGCAGGAT 180


31003R-1.IR_full       181 CGGCGCTTCAAGAATCGGGAACTGCAAATCATGCGCAAGCTGCGCCACGATAACATCATC 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| 
31003R-1.in silico     181 CGGCGCTTCAAGAATCGGGAACTGCAAATCATGCGCAAGCTGCGCCACGATAACATCATA 240


31003R-1.IR_full       241 ACTCTCAAGTGGTTCTTCTTTTCAAGTGGCGAGAAAAGGGACGAGGTCTATCTGAACCTG 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31003R-1.in silico     241 ACTCTCAAGTGGTTCTTCTTTTCAAGTGGCGAGAAAAGGGACGAGGTCTATCTGAACCTG 300


31003R-1.IR_full       301 GTCATGGAGTTCTTGCCGGAGACGCTGTATAAAGTGGAGCGACAGTACGCAAGGGCAAAG 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31003R-1.in silico     301 GTCATGGAGTTCTTGCCGGAGACGCTGTATAAAGTGGAGCGACAGTACGCAAGGGCAAAG 360


31003R-1.IR_full       361 CAAACCTTGCCAGTTAACTTTGTCCGACTTTACATGTACCAGCTGCTGAGGAGCATGGGA 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31003R-1.in silico     361 CAAACCTTGCCAGTTAACTTTGTCCGACTTTACATGTACCAGCTGCTGAGGAGCATGGGA 420


31003R-1.IR_full       421 TATCTCCACAGCTTGGGATTCTGTCACAGGGACATCAAGCCGCAGAACATGCTGCTCGAT 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31003R-1.in silico     421 TATCTCCACAGCTTGGGATTCTGTCACAGGGACATCAAGCCGCAGAACATGCTGCTCGAT 480


31003R-1.IR_full       481 TCGGAGACGGGTGTGCTTAAG 501
                           |||||||||||||||||||||
31003R-1.in silico     481 TCGGAGACGGGTGTGCTTAAG 501



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_170547.2  CG31003-RA (gskt), mRNA 
0.41   2  6  26  35  NM_057366.3  CG2621-RA, transcript variant A (sgg), mRNA 
0.41   2  6  26  35  NM_206613.1  CG2621-RJ, transcript variant J (sgg), mRNA 
0.41   2  6  26  35  NM_166948.1  CG2621-RF, transcript variant F (sgg), mRNA 
0.41   2  6  26  35  NM_166947.1  CG2621-RE, transcript variant E (sgg), mRNA 
0.41   2  6  26  35  NM_057367.3  CG2621-RB, transcript variant B (sgg), mRNA 
0.41   2  6  26  35  NM_206614.1  CG2621-RI, transcript variant I (sgg), mRNA 
0.41   2  6  26  35  NM_206615.1  CG2621-RH, transcript variant H (sgg), mRNA 
0.41   2  6  26  35  NM_134278.2  CG2621-RC, transcript variant C (sgg), mRNA 
0.41   2  6  26  35  NM_206612.1  CG2621-RG, transcript variant G (sgg), mRNA 
0.41   2  6  26  35  NM_206611.1  CG2621-RK, transcript variant K (sgg), mRNA 
0.41   2  6  26  35  NM_057368.3  CG2621-RD, transcript variant D (sgg), mRNA 
0.41   2  6  26  35  NM_001042794.1  CG2621-RL, transcript variant L (sgg), mRNA 
0.2   1  3  5  3  NM_079113.2  CG4012-RA (gek), mRNA 
0   0  9  4  4  NM_167288.1  CG32666-RB, transcript variant B (CG32666), mRNA 
0   0  9  4  4  NM_206688.1  CG32666-RA, transcript variant A (CG32666), mRNA 
0   0  7  11  4  NM_057401.3  CG3068-RA (aur), mRNA 
0   0  4  5  4  NM_079940.4  CG16973-RA, transcript variant A (msn), mRNA 
0   0  4  5  4  NM_206252.2  CG16973-RB, transcript variant B (msn), mRNA 
0   0  4  5  4  NM_206251.2  CG16973-RD, transcript variant D (msn), mRNA 
0   0  4  5  4  NM_206250.2  CG16973-RC, transcript variant C (msn), mRNA 
0   0  4  5  3  NM_206249.2  CG16973-RE, transcript variant E (msn), mRNA 
0   0  3  8  5  NM_140327.1  CG10522-RA (sti), mRNA 
0   0  2  8  6  NM_135106.1  CG7236-RA (CG7236), mRNA 
0   0  2  5  2  NM_143446.2  CG1907-RA (CG1907), mRNA 
0   0  2  4  4  NM_144350.1  CG7487-RA (RecQ4), mRNA 
0   0  2  3  9  NM_176397.2  CG32944-RB, transcript variant B (CG32944), mRNA 
0   0  2  3  9  NM_001043201.1  CG32944-RC, transcript variant C (CG32944), mRNA 
0   0  2  3  4  NM_134774.2  CG17646-RA, transcript variant A (CG17646), mRNA 
0   0  2  3  4  NM_164436.1  CG17646-RB, transcript variant B (CG17646), mRNA 

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