NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 3092R-1

Symbol CG3092 Full Name CG3092

CG No CG3092 Old CG No CG3092

Synonyms anon-WO0140519.90, CG3092

Accession No (Link to NCBI) NM_137926.2

Inserted Chr. lll

Insertional Mutation lethal

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
[Please submit your publication] Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgTATTCGGCAATCACCATCCGC

Primer Seq. 3'

aatctagaggtaccTGGCCACCTTCATGGGAATCG

Predicted Fragment Size

IR fragment full Seq

0001 TATTCGGCAA TCACCATCCG CATAACACCC ATACGCGCAA TCCGAGCTGC CCTTGGAAGT 
0061 CGGAGTGCGG ATGCGGGCAC GGATGCGTTT GTGGCGACGT CCCCGGCGAT TCGCAGGTGA 
0121 TCCGCCGTGG AATACACGAC TGGGATCGGC GCANGTGCGG caCACCNACG AGCGCCGTAA 
0181 GATCATTCCG AAACTGGTGT ATTTGCACAA TCCGTGGAAG TACCTGGTGA CCAAGTTCAA 
0241 TCTGTGGAAG CTTAAGTGGC TCTGGGATCG GGAGTTTAGC GAGCCTGACT TCTTGGAGGG 
0301 CGCCAAGCAG GCGGCCATCG TGATGACGGA CATCATCCGT CAGCAGAGTG CCGATAAGAT 
0361 TAGCGAGTAT ACGACACCGG TGGGCTTCCA GCAGATCACC CGGGACATGC TGCTATCCCG 
0421 CAACGACACG CGCCTCCAGC TGGTCAGGTT CGAGCGGGAG CACCTGNGCC GCGCGATTCC 
0481 CATGAAGGTG GCCA

in silico PCR Fragment

0001 TATTCGGCAA TCACCATCCG CATAACACCC ATACGCGCAA TCCGAGCTGC CCTTGGAAGT 
0061 CGGAGTGCGG ATGCGGGCAC GGATGCGTTT GTGGCGACGT CCCCGGCGAT TCGCAGGTGA 
0121 TCTGCCGCGG AATACACGAC TGGGATCGGC GCA-GTGCGG CACACC-ACG AGCGCCGTAA 
0181 GATCATTCCG AAACTGGTGT ATTTGCACAA TCCGTGGAAG TACCTGGTGA CCAAGTTCAA 
0241 TCTGTGGAAG CTTAAGTGGC TCTGGGATCG GGAGTTTAGC GAGCCTGACT TCTTGGAGGG 
0301 CGCCAAGCAG GCGGCCATCG TGATGACGGA CATCATCCGT CAGCAGAGTG CCGATAAGAT 
0361 TAGCGAGTAT ACGACACCGG TGGGCTTCCA GCAGATCACC CGGGACATGC TGCTATCCCG 
0421 CAACGACACG CGCCTCCAGC TGGTCAGGTT CGAGCGGGAG CACCTGCGCC GCGCGATTCC 
0481 CATGAAGGTG GCCA

Assemble Data


3092R-1.IR_full       1   TATTCGGCAATCACCATCCGCATAACACCCATACGCGCAATCCGAGCTGCCCTTGGAAGT 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3092R-1.in silico     1   TATTCGGCAATCACCATCCGCATAACACCCATACGCGCAATCCGAGCTGCCCTTGGAAGT 60


3092R-1.IR_full       61  CGGAGTGCGGATGCGGGCACGGATGCGTTTGTGGCGACGTCCCCGGCGATTCGCAGGTGA 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3092R-1.in silico     61  CGGAGTGCGGATGCGGGCACGGATGCGTTTGTGGCGACGTCCCCGGCGATTCGCAGGTGA 120


3092R-1.IR_full       121 TCCGCCGTGGAATACACGACTGGGATCGGCGCANGTGCGGCACACCNACGAGCGCCGTAA 180
                          || |||| ||||||||||||||||||||||||| |||||||||||| |||||||||||||
3092R-1.in silico     121 TCTGCCGCGGAATACACGACTGGGATCGGCGCA-GTGCGGCACACC-ACGAGCGCCGTAA 180


3092R-1.IR_full       181 GATCATTCCGAAACTGGTGTATTTGCACAATCCGTGGAAGTACCTGGTGACCAAGTTCAA 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3092R-1.in silico     181 GATCATTCCGAAACTGGTGTATTTGCACAATCCGTGGAAGTACCTGGTGACCAAGTTCAA 240


3092R-1.IR_full       241 TCTGTGGAAGCTTAAGTGGCTCTGGGATCGGGAGTTTAGCGAGCCTGACTTCTTGGAGGG 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3092R-1.in silico     241 TCTGTGGAAGCTTAAGTGGCTCTGGGATCGGGAGTTTAGCGAGCCTGACTTCTTGGAGGG 300


3092R-1.IR_full       301 CGCCAAGCAGGCGGCCATCGTGATGACGGACATCATCCGTCAGCAGAGTGCCGATAAGAT 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3092R-1.in silico     301 CGCCAAGCAGGCGGCCATCGTGATGACGGACATCATCCGTCAGCAGAGTGCCGATAAGAT 360


3092R-1.IR_full       361 TAGCGAGTATACGACACCGGTGGGCTTCCAGCAGATCACCCGGGACATGCTGCTATCCCG 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3092R-1.in silico     361 TAGCGAGTATACGACACCGGTGGGCTTCCAGCAGATCACCCGGGACATGCTGCTATCCCG 420


3092R-1.IR_full       421 CAACGACACGCGCCTCCAGCTGGTCAGGTTCGAGCGGGAGCACCTGNGCCGCGCGATTCC 480
                          |||||||||||||||||||||||||||||||||||||||||||||| |||||||||||||
3092R-1.in silico     421 CAACGACACGCGCCTCCAGCTGGTCAGGTTCGAGCGGGAGCACCTGCGCCGCGCGATTCC 480


3092R-1.IR_full       481 CATGAAGGTGGCCA 494
                          ||||||||||||||
3092R-1.in silico     481 CATGAAGGTGGCCA 494

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	474	0	0	0	NM_137926.2	CG3092-RA (CG3092), mRNA
0	0	6	3	8	NM_206374.1	CG7450-RB, transcript variant B (CrebA), mRNA
0	0	6	3	8	NM_079363.3	CG7450-RA, transcript variant A (CrebA), mRNA
0	0	5	2	11	NM_080020.3	CG11491-RE, transcript variant E (br), mRNA
0	0	5	2	11	NM_166898.1	CG11491-RG, transcript variant G (br), mRNA
0	0	5	2	5	NM_079181.2	CG1065-RA (Scsalpha), mRNA
0	0	3	5	2	NM_142182.1	CG14864-RA, transcript variant A (CG14864), mRNA
0	0	3	5	2	NM_001043249.1	CG14864-RB, transcript variant B (CG14864), mRNA
0	0	2	3	5	NM_143582.1	CG15552-RA (Sox100B), mRNA
0	0	2	3	3	NM_140175.2	CG6297-RB, transcript variant B (JIL-1), mRNA
0	0	1	4	3	NM_142583.2	CG4703-RA (Arc42), mRNA
0	0	1	3	2	NM_140968.2	CG11796-RB, transcript variant B (CG11796), mRNA
0	0	1	3	2	NM_168857.1	CG11796-RA, transcript variant A (CG11796), mRNA
0	0	1	2	8	NM_139584.1	CG14982-RB (CG14982), mRNA
0	0	1	2	5	NM_078601.2	CG9533-RA (rut), mRNA
0	0	1	2	4	NM_057391.3	CG1977-RA (alpha-Spec), mRNA
0	0	1	2	2	NM_165403.1	CG31601-RA (CG31601), mRNA
0	0	1	1	4	NM_133004.1	CG8211-RA (CG8211), mRNA
0	0	0	8	7	NM_137990.2	CG4797-RA, transcript variant A (CG4797), mRNA
0	0	0	8	7	NM_166625.1	CG4797-RB, transcript variant B (CG4797), mRNA
0	0	0	5	5	NM_078878.2	CG10123-RA (Top3alpha), mRNA
0	0	0	5	4	NM_132794.2	CG18210-RA (CG18210), mRNA
0	0	0	4	8	NM_140803.1	CG14073-RB, transcript variant B (CG14073), mRNA
0	0	0	4	5	NM_167041.1	CG3187-RA, transcript variant A (Sirt4), mRNA
0	0	0	4	5	NM_132013.2	CG3187-RC, transcript variant C (Sirt4), mRNA
0	0	0	4	5	NM_132122.2	CG3126-RA, transcript variant A (C3G), mRNA
0	0	0	4	5	NM_176694.1	CG3126-RB, transcript variant B (C3G), mRNA
0	0	0	4	5	NM_167042.2	CG3187-RB, transcript variant B (Sirt4), mRNA
0	0	0	4	4	NM_057691.3	CG4654-RA, transcript variant A (Dp), mRNA
0	0	0	4	4	NM_144269.2	CG3576-RB, transcript variant B (Lag1), mRNA

Wing Disc

GFP

Puc

Caspase

DAPI

Merge

Figure

JNK signal

Caspase-3 signal

Dcompartment		Penetrance

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