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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
3066R-3 |
| Symbol |
Sp7 |
Full Name |
Serine protease 7 |
| CG No |
CG3066 |
Old CG No |
CG3066 |
| Synonyms |
MP2, CG3066, SP7, proPO-AE, anon-Ryu, Sp7 |
| Accession No (Link to NCBI) |
NM_141477.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
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Reference
[Please submit your publication]
|
Kambris Z, Brun S, Jang IH, Nam HJ, Romeo Y, Takahashi K, Lee WJ, Ueda R, Lemaitre B.
Drosophila immunity: a large-scale in vivo RNAi screen identifies five serine proteases required for Toll activation.
Curr Biol (2006) 16(8) 808-13
[
PubMed ID = 16631589
]
[
RRC reference
]
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| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgCCCAACCAGAAACAGGGACAA |
| Primer Seq. 3' |
aatctagaggtaccAACCGAACGGTAGTTAGGTGA |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 CCCAACCAGA AACAGGGACA ATGTTTGTCC ATATACGACT GTCAAAGTCT GCTTTCCGTG
0061 ATTCAGCAGT CTTATGTCAG CCCGGAGGAC AGAACCTTTC TTCGAAACTC ACAGTGCCTC
0121 GATGGAGTAG GTCGCCAGCC ATATGTTTGC TGTACAAGTG ATCGCAGCTT TGGATCCCAG
0181 GAGGCCACCT CCGCAGCCCC TCCTCCAACG ACTACCAGTT CAAGTTCACG AGGTCAAGAC
0241 GGTCAAGCGG GTCTGGGCAA CCTGCTGCCC TCGCCACCCA AGTGCGGGCC CCACTCCTTC
0301 AGCAATAAGG TCTATAATGG CAACGACACT GCTATTGACG AGTTCAACTG GATGGCACTG
0361 CNTTGAGTAC GTGGATAACA GGGGACGGCG GGAACTCAGT TGCGGTGGCA GTTTGATCAA
0421 CAATCGTTAC GTTCTTACCG CCGCCCATTG CGTCATTGGT GCGGTGGAAA CGGAAGTAGG
0481 TCACCTANCT ANCGTTCGGT T |
| in silico PCR Fragment |
0001 CCCAACCAGA AACAGGGACA ATGTTTGTCC ATATACGACT GTCAAAGTCT GCTTTCCGTG
0061 ATTCAGCAGT CTTATGTCAG CCCGGAGGAC AGAACCTTTC TTCGAAACTC ACAGTGCCTC
0121 GATGGAGTAG GTCGCCAGCC ATATGTTTGC TGTACAAGTG ATCGCAGCTT TGGATCCCAG
0181 GAGGCCACCT CCGCAGCCCC TCCTCCAACG ACTACCAGTT CAAGTTCACG AGGTCAAGAC
0241 GGTCAAGCGG GTCTGGGCAA CCTGCTGCCC TCGCCACCCA AGTGCGGGCC CCACTCCTTC
0301 AGCAATAAGG TCTATAATGG CAACGACACT GCTATTGACG AGTTCAACTG GATGGCACTG
0361 C-TTGAGTAC GTGGATAACA GGGGACGGCG GGAACTCAGT TGCGGTGGCA GTTTGATCAA
0421 CAATCGTTAC GTTCTTACCG CCGCCCATTG CGTCATTGGT GCGGTGGAAA CGGAAGTAGG
0481 TCACCTAACT ACCGTTCGGT T |
| Assemble Data |
3066R-3.IR_full 1 CCCAACCAGAAACAGGGACAATGTTTGTCCATATACGACTGTCAAAGTCTGCTTTCCGTG 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3066R-3.in silico 1 CCCAACCAGAAACAGGGACAATGTTTGTCCATATACGACTGTCAAAGTCTGCTTTCCGTG 60
3066R-3.IR_full 61 ATTCAGCAGTCTTATGTCAGCCCGGAGGACAGAACCTTTCTTCGAAACTCACAGTGCCTC 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3066R-3.in silico 61 ATTCAGCAGTCTTATGTCAGCCCGGAGGACAGAACCTTTCTTCGAAACTCACAGTGCCTC 120
3066R-3.IR_full 121 GATGGAGTAGGTCGCCAGCCATATGTTTGCTGTACAAGTGATCGCAGCTTTGGATCCCAG 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3066R-3.in silico 121 GATGGAGTAGGTCGCCAGCCATATGTTTGCTGTACAAGTGATCGCAGCTTTGGATCCCAG 180
3066R-3.IR_full 181 GAGGCCACCTCCGCAGCCCCTCCTCCAACGACTACCAGTTCAAGTTCACGAGGTCAAGAC 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3066R-3.in silico 181 GAGGCCACCTCCGCAGCCCCTCCTCCAACGACTACCAGTTCAAGTTCACGAGGTCAAGAC 240
3066R-3.IR_full 241 GGTCAAGCGGGTCTGGGCAACCTGCTGCCCTCGCCACCCAAGTGCGGGCCCCACTCCTTC 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3066R-3.in silico 241 GGTCAAGCGGGTCTGGGCAACCTGCTGCCCTCGCCACCCAAGTGCGGGCCCCACTCCTTC 300
3066R-3.IR_full 301 AGCAATAAGGTCTATAATGGCAACGACACTGCTATTGACGAGTTCAACTGGATGGCACTG 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3066R-3.in silico 301 AGCAATAAGGTCTATAATGGCAACGACACTGCTATTGACGAGTTCAACTGGATGGCACTG 360
3066R-3.IR_full 361 CNTTGAGTACGTGGATAACAGGGGACGGCGGGAACTCAGTTGCGGTGGCAGTTTGATCAA 420
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3066R-3.in silico 361 C-TTGAGTACGTGGATAACAGGGGACGGCGGGAACTCAGTTGCGGTGGCAGTTTGATCAA 420
3066R-3.IR_full 421 CAATCGTTACGTTCTTACCGCCGCCCATTGCGTCATTGGTGCGGTGGAAACGGAAGTAGG 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3066R-3.in silico 421 CAATCGTTACGTTCTTACCGCCGCCCATTGCGTCATTGGTGCGGTGGAAACGGAAGTAGG 480
3066R-3.IR_full 481 TCACCTANCTANCGTTCGGTT 501
||||||| ||| |||||||||
3066R-3.in silico 481 TCACCTAACTACCGTTCGGTT 501
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
478 |
0 |
0 |
0 |
NM_206457.1 |
CG3066-RD, transcript variant D (Sp7), mRNA |
| 96.23 |
460 |
2 |
2 |
2 |
NM_169192.2 |
CG3066-RC, transcript variant C (Sp7), mRNA |
| 96.23 |
460 |
2 |
2 |
2 |
NM_141477.2 |
CG3066-RA, transcript variant A (Sp7), mRNA |
| 96.23 |
460 |
2 |
2 |
2 |
NM_169193.1 |
CG3066-RB, transcript variant B (Sp7), mRNA |
| 0 |
0 |
2 |
9 |
2 |
NM_167535.1 |
CG9676-RA (CG9676), mRNA |
| 0 |
0 |
2 |
6 |
7 |
NM_141193.3 |
CG1102-RA (MP1), mRNA |
| 0 |
0 |
2 |
4 |
4 |
NM_079195.2 |
CG15002-RB (mas), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_057385.4 |
CG11121-RA (so), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_136421.1 |
CG1850-RA (CG1850), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_138193.1 |
CG17180-RA (CG17180), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_136775.2 |
CG12344-RA (CG12344), mRNA |
| 0 |
0 |
1 |
5 |
4 |
NM_132922.2 |
CG4653-RA (CG4653), mRNA |
| 0 |
0 |
1 |
4 |
4 |
NM_132704.1 |
CG11071-RA (CG11071), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_170015.1 |
CG7057-RA, transcript variant A (AP-50), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_142792.3 |
CG7057-RB, transcript variant B (AP-50), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_079503.2 |
CG9761-RA (Nep2), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_141762.2 |
CG14692-RA (CG14692), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_206420.1 |
CG33291-RA (CG33291), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_141594.1 |
CG11970-RA (CG11970), mRNA |
| 0 |
0 |
0 |
7 |
2 |
NM_143314.2 |
CG5634-RA (dsd), mRNA |
| 0 |
0 |
0 |
6 |
5 |
NM_137872.2 |
CG13527-RA (CG13527), mRNA |
| 0 |
0 |
0 |
6 |
4 |
NM_136265.2 |
CG17571-RA (CG17571), mRNA |
| 0 |
0 |
0 |
5 |
6 |
NM_057361.3 |
CG4316-RA (Sb), mRNA |
| 0 |
0 |
0 |
5 |
5 |
NM_142346.1 |
CG5246-RA (CG5246), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_132054.1 |
CG6048-RA (CG6048), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_057269.2 |
CG10236-RA (LanA), mRNA |
| 0 |
0 |
0 |
5 |
2 |
NM_078969.3 |
CG12385-RA (thetaTry), mRNA |
| 0 |
0 |
0 |
4 |
11 |
NM_001038734.1 |
CG16902-RC (Hr4), mRNA |
| 0 |
0 |
0 |
4 |
11 |
NM_137945.1 |
CG30414-RA (CG30414), mRNA |
| 0 |
0 |
0 |
4 |
9 |
NM_141909.2 |
CG12256-RA (CG12256), mRNA |
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