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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 30482R-3 
 Symbol CG30482  Full Name CG30482 
 CG No CG30482  Old CG No CG30482 
 Synonyms CG30482 
 Accession No (Link to NCBI) NM_166029.1 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgGAAGGCTGAAAGTCGTCGGCT 
 Primer Seq. 3' 
aatctagaggtaccTGGCTTGGATTTTGGCCCGGA 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 GAAGGCTGAA AGTCGTCGGC TGGCTGAAAC GTCTGTTGGC CCATGAGAAA ACGACGCCAA 
0061 ACAAAATGGT GCCGCCTCCA GTGACGAAAG CGACAAGCCA ACTAACTAGC CAAGTAACTA 
0121 ACAACCAACA AGCAACGACT AACGACAAAC GCAATACACA AAGAGAACGA CAAACGATGG 
0181 CGGCGACAAA AAGTCAATTT TATCGGAAAC TAANCAGGCT GCAAGCAGCC GGAGTAAAGC 
0241 AAAGCAAAGC TGGGACGGAA AAGCCAAGGC GGACTTTCCA CTTTTCGTTT GGCTGGCCTG 
0301 GAAAAGCTCT CTTTCTCTAT TGCCTCTATG GCTCGGNAGC GcTGGGCGCC TAATCCGAAT 
0361 TGCGAGAAAG ATCCGCTTTT CGAGCCAAAA ACCNTCGAAA AGCCTGGCAA GCACACAGCT 
0421 CGCACAAATT TTCCGCCCAA AAgNCACGCC CCCGAAATTT GGAGTGCCGC CCACCTGGAT 
0481 GTCCGGGCCA AAATCCAAGC CA 
 in silico PCR Fragment 
0001 GAAGGCTGAA AGTCGTCGGC TGGCTGAAAC GTCTGTTGGC CCATGAGAAA ACGACGCCAA 
0061 ACAAAATGGT GCCGCCTCCA GTGACGAAAG CGACAAGCCA ACTAACTAGC CAAGTAACTA 
0121 ACAACCAACA ACCAACGACT AACGACAAAC GCAACACACA AAGAGAACGA CAAACGATGG 
0181 CGGCGACAAA AAGTCAATTT TATCGGAAAC TAA-CAGGCT GCAAGCAGCC GGAG-AAAGC 
0241 AAAGCAAAGC TGGGACGGAA AAGCCAAGGC GGACTTTCCA CTTTTCGTTT GGCTGGCCTG 
0301 GAAAAGCTCT CTTTCTCTAT TGCCTCTATG GCTCGGCAGC GCTGGGCGCC TAATCCGAAT 
0361 TGCGAGAAAG ATCCGCTTTT CGAGCCAAAA ACCATCGAAA AGCCTGGCAA GCACACAGCT 
0421 CGCACAAATT TTCCGCCCAA AAGCCACGCC CCCGAAATTT GGAGTGCCGC CCACCTGGAT 
0481 GTCCGGGCCA AAATCCAAGC CA 
 Assemble Data 

30482R-3.IR_full       1   GAAGGCTGAAAGTCGTCGGCTGGCTGAAACGTCTGTTGGCCCATGAGAAAACGACGCCAA 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
30482R-3.in silico     1   GAAGGCTGAAAGTCGTCGGCTGGCTGAAACGTCTGTTGGCCCATGAGAAAACGACGCCAA 60


30482R-3.IR_full       61  ACAAAATGGTGCCGCCTCCAGTGACGAAAGCGACAAGCCAACTAACTAGCCAAGTAACTA 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
30482R-3.in silico     61  ACAAAATGGTGCCGCCTCCAGTGACGAAAGCGACAAGCCAACTAACTAGCCAAGTAACTA 120


30482R-3.IR_full       121 ACAACCAACAAGCAACGACTAACGACAAACGCAATACACAAAGAGAACGACAAACGATGG 180
                           ||||||||||| |||||||||||||||||||||| |||||||||||||||||||||||||
30482R-3.in silico     121 ACAACCAACAACCAACGACTAACGACAAACGCAACACACAAAGAGAACGACAAACGATGG 180


30482R-3.IR_full       181 CGGCGACAAAAAGTCAATTTTATCGGAAACTAANCAGGCTGCAAGCAGCCGGAGTAAAGC 240
                           ||||||||||||||||||||||||||||||||| |||||||||||||||||||| |||||
30482R-3.in silico     181 CGGCGACAAAAAGTCAATTTTATCGGAAACTAA-CAGGCTGCAAGCAGCCGGAG-AAAGC 240


30482R-3.IR_full       241 AAAGCAAAGCTGGGACGGAAAAGCCAAGGCGGACTTTCCACTTTTCGTTTGGCTGGCCTG 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
30482R-3.in silico     241 AAAGCAAAGCTGGGACGGAAAAGCCAAGGCGGACTTTCCACTTTTCGTTTGGCTGGCCTG 300


30482R-3.IR_full       301 GAAAAGCTCTCTTTCTCTATTGCCTCTATGGCTCGGNAGCGCTGGGCGCCTAATCCGAAT 360
                           |||||||||||||||||||||||||||||||||||| |||||||||||||||||||||||
30482R-3.in silico     301 GAAAAGCTCTCTTTCTCTATTGCCTCTATGGCTCGGCAGCGCTGGGCGCCTAATCCGAAT 360


30482R-3.IR_full       361 TGCGAGAAAGATCCGCTTTTCGAGCCAAAAACCNTCGAAAAGCCTGGCAAGCACACAGCT 420
                           ||||||||||||||||||||||||||||||||| ||||||||||||||||||||||||||
30482R-3.in silico     361 TGCGAGAAAGATCCGCTTTTCGAGCCAAAAACCATCGAAAAGCCTGGCAAGCACACAGCT 420


30482R-3.IR_full       421 CGCACAAATTTTCCGCCCAAAAGNCACGCCCCCGAAATTTGGAGTGCCGCCCACCTGGAT 480
                           ||||||||||||||||||||||| ||||||||||||||||||||||||||||||||||||
30482R-3.in silico     421 CGCACAAATTTTCCGCCCAAAAGCCACGCCCCCGAAATTTGGAGTGCCGCCCACCTGGAT 480


30482R-3.IR_full       481 GTCCGGGCCAAAATCCAAGCCA 502
                           ||||||||||||||||||||||
30482R-3.in silico     481 GTCCGGGCCAAAATCCAAGCCA 502



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  4  18  NM_166029.1  CG30482-RA (CG30482), mRNA 
0   0  6  2  3  NM_143433.1  CG14506-RA (CG14506), mRNA 
0   0  3  4  2  NM_138154.2  CG32475-RA (mthl8), mRNA 
0   0  2  3  3  NM_135693.1  CG6766-RA (CG6766), mRNA 
0   0  1  4  8  NM_134860.2  CG9961-RA (CG9961), mRNA 
0   0  1  4  5  NM_080250.2  CG6224-RA (dbo), mRNA 
0   0  1  3  6  NM_140637.2  CG4573-RA (CG4573), mRNA 
0   0  1  3  4  NM_133099.1  CG7058-RA (CG7058), mRNA 
0   0  1  3  4  NM_078596.2  CG1372-RA, transcript variant A (yl), mRNA 
0   0  1  3  4  NM_206710.1  CG1372-RB, transcript variant B (yl), mRNA 
0   0  1  3  2  NM_142766.1  CG5386-RA (CG5386), mRNA 
0   0  1  2  4  NM_170318.1  CG5896-RA, transcript variant A (CG5896), mRNA 
0   0  1  2  4  NM_143286.1  CG5896-RB, transcript variant B (CG5896), mRNA 
0   0  1  2  3  NM_057958.3  CG3584-RA (qkr58E-3), mRNA 
0   0  1  2  2  NM_206018.1  CG33317-RA (CG33317), mRNA 
0   0  1  2  2  NM_139596.2  CG1136-RA (CG1136), mRNA 
0   0  0  7  6  NM_167119.2  CG4626-RB, transcript variant B (fz4), mRNA 
0   0  0  7  4  NM_078513.2  CG4626-RA, transcript variant A (fz4), mRNA 
0   0  0  5  10  NM_078856.2  CG4838-RA (beat-Ic), mRNA 
0   0  0  5  7  NM_164577.1  CG15427-RC, transcript variant C (tutl), mRNA 
0   0  0  5  5  NM_175973.1  CG33113-RA, transcript variant A (Rtnl1), mRNA 
0   0  0  5  3  NM_166950.1  CG2694-RC, transcript variant C (CG2694), mRNA 
0   0  0  5  3  NM_130668.2  CG2694-RB, transcript variant B (CG2694), mRNA 
0   0  0  5  3  NM_166949.1  CG2694-RA, transcript variant A (CG2694), mRNA 
0   0  0  5  3  NM_138181.1  CG13882-RA (CG13882), mRNA 
0   0  0  5  2  NM_136637.1  CG13954-RA (CG13954), mRNA 
0   0  0  4  10  NR_001953.1  CR32028, miscRNA 
0   0  0  4  6  NM_078645.2  CG4252-RA (mei-41), mRNA 
0   0  0  4  6  NM_165325.1  CG10076-RC, transcript variant C (spir), mRNA 
0   0  0  4  5  NM_165323.1  CG10076-RA, transcript variant A (spir), mRNA 
 Wing Disc
GFP
 Puc
 Caspase
DAPI
 Merge
 Figure
JNK signal
N
 
DB
 
DC
 
DM
 
VM
 
VB
 
Caspase-3 signal
N
+ 
DB
+ 
DC
++ 
DM
+ 
VM
+ 
VB
 
Dcompartment   Penetrance
     

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