NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 30475R-1

Symbol CG30475 Full Name CG30475

CG No CG30475 Old CG No CG30475

Synonyms CG30475

Accession No (Link to NCBI) NM_166075.1

Inserted Chr. ll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
[Please submit your publication] Cho Y, Lai CM, Lin KY, Hsu HJ.
A Targeted RNAi Screen Reveals Drosophila Female-Sterile Genes That Control the Size of Germline Stem Cell Niche During Development.
G3 (Bethesda) (2018) 8(7) 2345-2354 [ PubMed ID = 29764959 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGGCTATTGGTCCGCCACGACT

Primer Seq. 3'

aatctagaggtaccCCACGGGTCAGCGTGTTGTTC

Predicted Fragment Size

IR fragment full Seq

0001 gGNTANTTGG TCCGCCACGA CTGTGNATCG TGGACGATCG CCGCGCCGCC TGACGCAATG 
0061 TGCACCACTT CCGGTCAACA TCGTGCAGCA GGAGGAGGAG GAGGAGCAGC CCTGCCTGCC 
0121 GCAACAGCAG CAGCATCAGC AAACGCAGCA GCAACAGCAA TCGCAGACCT GCCCCAGCAC 
0181 ACCGTCGGCC AATCAGCAGG GCAGTGGCAA CTTGTGCTAC AGCCCCACCT GTCGATCGCG 
0241 CTGCTCGAGT CCTTGTCCGG GATCTNCCGT GCGGCTCCAT TACGCCGCCA CCTCCGCCGA 
0301 TGCTCACGTC CTCGCAGCAG CACCTGCATT CCAATAAGAA TTGCCCAGCG GCGCAGCAAC 
0361 TGATGACCCA CTTGGATTAC GCCGCTCGGA GACAATCGCT CGACCAGCTG GATAGTCCAC 
0421 AGGCCAACCA GCTGTCGGAC ATCATGNTGc cNCGGAGCGG TGGTCAGTTC CATTCAGTCG 
0481 GCGAACAACA CGCTGANCCG TGG

in silico PCR Fragment

0001 GGCTA-TTGG TCCGCCACGA CTGTGGATCG TGGACGATCG CCGCGCCGCC TGACGCAATG 
0061 TGCACCACTT CCGGTCAACA TCGTGCAGCA GGAGGAGGAG GAGGAGCAGC CCTGCCTGCC 
0121 GCAACAGCAG CAGCATCAGC AAACGCAGCA GCAACAGCAA TCGCAGACCT GCCCCAGCAC 
0181 ACCGTCGGCC AATCAGCAGG GCAGTGGCAA CTTGTGCTAC AGCCCCACCT GTCGATCGCG 
0241 CTGCTCGAGT CCTTGTCCGG GATCT-CCGT GCGGCTCCAT TACGCCGCCA CCTCCGCCGA 
0301 TGCTCACGTC CTCGCAGCAG CACCTGCATT CCAATAAGAA TTGCCCAGCG GCGCAGCAAC 
0361 TGATGACCCA CTTGGATTAC GCCGCTCGGA GACAATCGCT CGACCAGCTG GATAGTCCAC 
0421 AGGCCAACCA GCTGTCGGAC ATCATG-TGC CGCGGAGCGG TGGTCAGTTC CATTCAGTCG 
0481 GCGAACAACA CGCTGACCCG TGG

Assemble Data


30475R-1.IR_full       1   GGNTANTTGGTCCGCCACGACTGTGNATCGTGGACGATCGCCGCGCCGCCTGACGCAATG 60
                           || || ||||||||||||||||||| ||||||||||||||||||||||||||||||||||
30475R-1.in silico     1   GGCTA-TTGGTCCGCCACGACTGTGGATCGTGGACGATCGCCGCGCCGCCTGACGCAATG 60


30475R-1.IR_full       61  TGCACCACTTCCGGTCAACATCGTGCAGCAGGAGGAGGAGGAGGAGCAGCCCTGCCTGCC 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
30475R-1.in silico     61  TGCACCACTTCCGGTCAACATCGTGCAGCAGGAGGAGGAGGAGGAGCAGCCCTGCCTGCC 120


30475R-1.IR_full       121 GCAACAGCAGCAGCATCAGCAAACGCAGCAGCAACAGCAATCGCAGACCTGCCCCAGCAC 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
30475R-1.in silico     121 GCAACAGCAGCAGCATCAGCAAACGCAGCAGCAACAGCAATCGCAGACCTGCCCCAGCAC 180


30475R-1.IR_full       181 ACCGTCGGCCAATCAGCAGGGCAGTGGCAACTTGTGCTACAGCCCCACCTGTCGATCGCG 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
30475R-1.in silico     181 ACCGTCGGCCAATCAGCAGGGCAGTGGCAACTTGTGCTACAGCCCCACCTGTCGATCGCG 240


30475R-1.IR_full       241 CTGCTCGAGTCCTTGTCCGGGATCTNCCGTGCGGCTCCATTACGCCGCCACCTCCGCCGA 300
                           ||||||||||||||||||||||||| ||||||||||||||||||||||||||||||||||
30475R-1.in silico     241 CTGCTCGAGTCCTTGTCCGGGATCT-CCGTGCGGCTCCATTACGCCGCCACCTCCGCCGA 300


30475R-1.IR_full       301 TGCTCACGTCCTCGCAGCAGCACCTGCATTCCAATAAGAATTGCCCAGCGGCGCAGCAAC 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
30475R-1.in silico     301 TGCTCACGTCCTCGCAGCAGCACCTGCATTCCAATAAGAATTGCCCAGCGGCGCAGCAAC 360


30475R-1.IR_full       361 TGATGACCCACTTGGATTACGCCGCTCGGAGACAATCGCTCGACCAGCTGGATAGTCCAC 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
30475R-1.in silico     361 TGATGACCCACTTGGATTACGCCGCTCGGAGACAATCGCTCGACCAGCTGGATAGTCCAC 420


30475R-1.IR_full       421 AGGCCAACCAGCTGTCGGACATCATGNTGCCNCGGAGCGGTGGTCAGTTCCATTCAGTCG 480
                           |||||||||||||||||||||||||| |||| ||||||||||||||||||||||||||||
30475R-1.in silico     421 AGGCCAACCAGCTGTCGGACATCATG-TGCCGCGGAGCGGTGGTCAGTTCCATTCAGTCG 480


30475R-1.IR_full       481 GCGAACAACACGCTGANCCGTGG 503
                           |||||||||||||||| ||||||
30475R-1.in silico     481 GCGAACAACACGCTGACCCGTGG 503

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	2	20	20	NM_166075.1	CG30475-RA (CG30475), mRNA
1.45	7	7	31	58	NM_132724.2	CG1810-RA (mRNA-capping-enzyme), mRNA
1.03	5	21	127	393	NM_206628.1	CG32767-RB, transcript variant B (CG32767), mRNA
1.03	5	21	127	393	NM_131975.3	CG32767-RA, transcript variant A (CG32767), mRNA
1.03	5	12	52	195	NM_166446.1	CG9696-RD, transcript variant D (dom), mRNA
1.03	5	12	52	195	NM_080094.2	CG9696-RA, transcript variant A (dom), mRNA
0.82	4	19	69	229	NM_078575.2	CG9355-RA (dy), mRNA
0.82	4	6	12	37	NM_080317.2	CG2647-RA (per), mRNA
0.62	3	38	51	72	NM_167524.2	CG9819-RB, transcript variant B (CanA-14F), mRNA
0.62	3	38	51	70	NM_167523.2	CG9819-RA, transcript variant A (CanA-14F), mRNA
0.62	3	16	26	43	NM_141658.3	CG8301-RA (CG8301), mRNA
0.62	3	12	29	103	NM_165233.1	CG5674-RC, transcript variant C (CG5674), mRNA
0.62	3	12	29	103	NM_136012.4	CG5674-RA, transcript variant A (CG5674), mRNA
0.62	3	12	29	103	NM_165232.1	CG5674-RB, transcript variant B (CG5674), mRNA
0.62	3	11	64	339	NM_078797.2	CG13109-RA (tai), mRNA
0.62	3	10	14	45	NM_141073.1	CG7173-RA (CG7173), mRNA
0.62	3	9	11	19	NM_134680.1	CG13691-RA (BBS8), mRNA
0.62	3	6	62	241	NM_206089.1	CG33473-RB (luna), mRNA
0.62	3	6	15	20	NM_001007096.1	CG8742-RB, transcript variant B (Gyc76C), mRNA
0.62	3	6	15	20	NM_079441.3	CG8742-RA, transcript variant A (Gyc76C), mRNA
0.62	3	6	15	20	NM_001007095.1	CG8742-RC, transcript variant C (Gyc76C), mRNA
0.62	3	4	11	98	NM_078563.2	CG18361-RA, transcript variant A (dsh), mRNA
0.62	3	4	11	98	NM_167279.1	CG18361-RB, transcript variant B (dsh), mRNA
0.62	3	4	10	83	NM_130728.1	CG15240-RA (CG15240), mRNA
0.62	3	3	20	89	NM_168039.1	CG32264-RB, transcript variant B (CG32264), mRNA
0.62	3	3	5	33	NM_206789.1	CG33253-RA (CG33253), mRNA
0.41	2	14	24	77	NM_167401.1	CG1517-RB, transcript variant B (na), mRNA
0.41	2	14	24	77	NM_167402.1	CG1517-RC, transcript variant C (na), mRNA
0.41	2	13	71	213	NM_134512.4	CG12701-RA, transcript variant A (CG12701), mRNA
0.41	2	13	71	213	NM_167685.1	CG12701-RB, transcript variant B (CG12701), mRNA

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