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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 30468R-2 
 Symbol CG30468  Full Name CG30468 
 CG No CG30468  Old CG No CG30468 
 Synonyms CG12961, CG30468 
 Accession No (Link to NCBI) NM_166098.1 
 Inserted Chr. ll 
 Insertional Mutation  lethal 
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgAGATTGCAAGTTCCTGCAGTG 
 Primer Seq. 3' 
aatctagaggtaccTGCATAGTGGCGTTAACCTTC 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 AGATTGCAAG TTCCTGCAGT GCTGGTGTCC TCAGGCAGCA CCAACTTTGA TTGGAATTTC 
0061 AGCAGCCTAA CACTGATACT CAGTTGTAAT TTCCAAGACG AGAGGGAAGA AAATTATCGA 
0121 ACACTGATGA AGCTGCAGAC TAGCAGGCGC CTAATCCTTC TGAAAGGACA TATAAAACCC 
0181 GAGTCTGTGT GCGATTTCTA TTCTAAAAAG GAGCAGCATA ATGTAGCCAT GGTGAAGGAA 
0241 AACTTCTATC AGCTTGAAGT GGTATACTCA TGTCGCCTAT TTCAAGATCA AAACTATGAA 
0301 AAACTGAATC TCTTCGATGG CAAATCGATT TACAAAGATC AATTTCGAAA TATGCATGGA 
0361 GCGCCAATAA GAACACTATC TGATAAGGAG CCACCCCGAA CCATACCATA TATTGATAGT 
0421 AAGACCGGTG AAGAAAAGTT TAAGGGCTAT GTGGGCATGT TGATAAGCCA ATTCGTTAAG 
0481 AAGGTTAACG CCACTATGCA  
 in silico PCR Fragment 
0001 AGATTGCAAG TTCCTGCAGT GCTGGTGTCC TCAGGCAGCA CCAACTTTGA TTGGAATTTC 
0061 AGCAGCCTAA CACTGATACT CAGTTGTAAT TTCCAAGACG AGAGGGAAGA AAATTATCGA 
0121 ACACTGATGA AGCTGCAGAC TAGCAGGCGC CTAATCCTTC TGAAAGGACA TATAAAACCC 
0181 GAGTCTGTGT GCGATTTCTA TTCTAAAAAG GAGCAGCATA ATGTAGCCAT GGTGAAGGAA 
0241 AACTTCTATC AGCTTGAAGT GGTATACTCA TGTCGCCTAT TTCAAGATCA AAACTATGAA 
0301 AAACTGAATC TCTTCGATGG CAAGTCGATT TACAAAGATC AATTTCGAAA TATGCATGGA 
0361 GCCCCAATAA GAACACTATC TGATAAGGAG CCACCCCGAA CCATACCATA TATTGATAGT 
0421 AAGACCGGTG AAGAAAAGTT TAAGGGCTAT GTGGGCATGT TAATAAGCCA ATTCGTTAAG 
0481 AAGGTTAACG CCACTATGCA  
 Assemble Data 

30468R-2.IR_full       1   AGATTGCAAGTTCCTGCAGTGCTGGTGTCCTCAGGCAGCACCAACTTTGATTGGAATTTC 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
30468R-2.in silico     1   AGATTGCAAGTTCCTGCAGTGCTGGTGTCCTCAGGCAGCACCAACTTTGATTGGAATTTC 60


30468R-2.IR_full       61  AGCAGCCTAACACTGATACTCAGTTGTAATTTCCAAGACGAGAGGGAAGAAAATTATCGA 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
30468R-2.in silico     61  AGCAGCCTAACACTGATACTCAGTTGTAATTTCCAAGACGAGAGGGAAGAAAATTATCGA 120


30468R-2.IR_full       121 ACACTGATGAAGCTGCAGACTAGCAGGCGCCTAATCCTTCTGAAAGGACATATAAAACCC 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
30468R-2.in silico     121 ACACTGATGAAGCTGCAGACTAGCAGGCGCCTAATCCTTCTGAAAGGACATATAAAACCC 180


30468R-2.IR_full       181 GAGTCTGTGTGCGATTTCTATTCTAAAAAGGAGCAGCATAATGTAGCCATGGTGAAGGAA 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
30468R-2.in silico     181 GAGTCTGTGTGCGATTTCTATTCTAAAAAGGAGCAGCATAATGTAGCCATGGTGAAGGAA 240


30468R-2.IR_full       241 AACTTCTATCAGCTTGAAGTGGTATACTCATGTCGCCTATTTCAAGATCAAAACTATGAA 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
30468R-2.in silico     241 AACTTCTATCAGCTTGAAGTGGTATACTCATGTCGCCTATTTCAAGATCAAAACTATGAA 300


30468R-2.IR_full       301 AAACTGAATCTCTTCGATGGCAAATCGATTTACAAAGATCAATTTCGAAATATGCATGGA 360
                           ||||||||||||||||||||||| ||||||||||||||||||||||||||||||||||||
30468R-2.in silico     301 AAACTGAATCTCTTCGATGGCAAGTCGATTTACAAAGATCAATTTCGAAATATGCATGGA 360


30468R-2.IR_full       361 GCGCCAATAAGAACACTATCTGATAAGGAGCCACCCCGAACCATACCATATATTGATAGT 420
                           || |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
30468R-2.in silico     361 GCCCCAATAAGAACACTATCTGATAAGGAGCCACCCCGAACCATACCATATATTGATAGT 420


30468R-2.IR_full       421 AAGACCGGTGAAGAAAAGTTTAAGGGCTATGTGGGCATGTTGATAAGCCAATTCGTTAAG 480
                           ||||||||||||||||||||||||||||||||||||||||| ||||||||||||||||||
30468R-2.in silico     421 AAGACCGGTGAAGAAAAGTTTAAGGGCTATGTGGGCATGTTAATAAGCCAATTCGTTAAG 480


30468R-2.IR_full       481 AAGGTTAACGCCACTATGCA 500
                           ||||||||||||||||||||
30468R-2.in silico     481 AAGGTTAACGCCACTATGCA 500



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_166098.1  CG30468-RA (CG30468), mRNA 
0.2   1  2  2  7  NM_138141.2  CG3770-RA (CG3770), mRNA 
0   0  7  22  17  NM_166097.1  CG30469-RA (CG30469), mRNA 
0   0  3  2  2  NM_164552.1  CG31955-RA (CG31955), mRNA 
0   0  2  3  2  NM_164876.1  CG31711-RA (CG31711), mRNA 
0   0  1  6  7  NM_165413.1  CG17018-RA, transcript variant A (CG17018), mRNA 
0   0  1  6  7  NM_165415.1  CG17018-RD, transcript variant D (CG17018), mRNA 
0   0  1  6  7  NM_165414.1  CG17018-RC, transcript variant C (CG17018), mRNA 
0   0  1  3  2  NM_167189.2  CG32705-RA (CG32705), mRNA 
0   0  1  2  7  NM_001015505.1  CG17866-PA.3 (CG17866), mRNA 
0   0  1  2  6  NM_079222.2  CG8339-RA (sfl), mRNA 
0   0  1  2  2  NM_168408.1  CG32065-RA (CG32065), mRNA 
0   0  0  14  64  NM_137198.1  CG30464-RA (CG30464), mRNA 
0   0  0  7  4  NM_131961.1  CG2861-RA, transcript variant A (CG2861), mRNA 
0   0  0  7  3  NM_167021.1  CG2861-RB, transcript variant B (CG2861), mRNA 
0   0  0  5  4  NM_132529.2  CG2025-RA (CG2025), mRNA 
0   0  0  5  2  NM_170430.2  CG31037-RA (ca), mRNA 
0   0  0  4  5  NM_132660.2  CG1716-RA (CG1716), mRNA 
0   0  0  4  3  NM_135799.2  CG9305-RA (CG9305), mRNA 
0   0  0  4  3  NM_139982.3  CG6638-RA (CG6638), mRNA 
0   0  0  4  2  NM_143609.2  CG31002-RA (CG31002), mRNA 
0   0  0  4  2  NM_057867.2  CG8808-RA (Pdk), mRNA 
0   0  0  3  8  NM_057511.3  CG3936-RA (N), mRNA 
0   0  0  3  7  NM_169560.1  CG2988-RA (ems), mRNA 
0   0  0  3  7  NM_137832.1  CG4554-RA (CG4554), mRNA 
0   0  0  3  5  NM_135347.1  CG8455-RB, transcript variant B (CG8455), mRNA 
0   0  0  3  5  NM_164798.1  CG8455-RA, transcript variant A (CG8455), mRNA 
0   0  0  3  4  NM_080325.3  CG6450-RC (lva), mRNA 
0   0  0  3  4  NM_132370.1  CG2989-RA (CG2989), mRNA 
0   0  0  3  2  NM_135604.2  CG12299-RA (CG12299), mRNA 

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