NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 3044R-4

Symbol CG3044 Full Name CG3044

CG No CG3044 Old CG No CG3044

Synonyms CG3044

Accession No (Link to NCBI) NM_132133.1

Inserted Chr. lll

Insertional Mutation lethal

Phenotype induced by Act5C-GAL4 at 28 degrees semi-lethal

Map Viewer

Reference
[Please submit your publication] Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624 [ PubMed ID = 21666802 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCCTGCGAACTTTGGTCTGCTG

Primer Seq. 3'

aatctagaggtaccGCTGCGAGAGATGCATTCGCT

Predicted Fragment Size

IR fragment full Seq

0001 CCTGCGAACT TTGGTCTGCT GGAGTGCTTT GTTGTTCTCT TTGTTGTGCC TTTGCCTCGG 
0061 GTTCATCGGC CTGGGATTAC TCGGCATCCA AACTGGGGAA GTGCACAAGG TGGGGCAGCG 
0121 ATTGGTGTGC TACTACGCCA GCGATGGCAC CCACAATCTC AGCCTTTTGG ATGTTCCCGG 
0181 CGATCTGTGC ACCCACATCA ATATCGGACC CGCCACTTTG GATAATGCCA CCATAGTCCT 
0241 TCCCGATAcC CTCAGACAGG TACTACAGAA CGACACCCGA TCCTTTCGTG CTGCCCATCC 
0301 GCAGGTTCAC CTGCTTCTCT GGATCGGTGG CGCCGACAGT GGGCGGTCAT TTGCCCTCAT 
0361 GGTgGCGAAC CACgCCATGC GAAAGTTGTT CCTGCGctNC CTCCGGGAAA TATTGCGCAC 
0421 CTATCCCAGT TTGGATGGCA TCGATCTCGA CTGGGAGTTT CCGAGTGCCT ACGATCGTGA 
0481 GcGAATGCAT CTCTCGCAGC

in silico PCR Fragment

0001 CCTGCGAACT TTGGTCTGCT GGAGTGCTTT GTTGTTCTCT TTGTTGTGCC TTTGCCTCGG 
0061 GTTCATCGGC CTGGGATTAC TCGGCATCCA AACTGGGGAA GTGCACAAGG TGGGGCAGCG 
0121 GTTGGTGTGC TACTACGCAA GCGATGGCAC CCACAATCTC AGCCTTTTGG ATGTTCCCGG 
0181 CGACCTGTGC ACCCACATCA ATATCGGACC CGCCACTTTG GATAATGCCA CCATAGTCCT 
0241 TCCCGATACC CTCAGACAGG TACTACAGAA CGACACCCGA TCCTTTCGTG CTGCCCATCC 
0301 GCAGGTTCAC CTGCTTCTCT GGATCGGTGG CGCCGACAGT GGGCGGTCAT TTGCCCTCAT 
0361 GGTGGCGAAC CACGCCATGC GAAAGTTGTT CCTGCGCTCC CTCCGGGAAA TATTGCGCAC 
0421 CTATCCCAGT TTGGATGGCA TCGATCTCGA CTGGGAGTTT CCGAGTGCCT ACGATCGTGA 
0481 GCGAATGCAT CTCTCGCAGC

Assemble Data


3044R-4.IR_full       1   CCTGCGAACTTTGGTCTGCTGGAGTGCTTTGTTGTTCTCTTTGTTGTGCCTTTGCCTCGG 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3044R-4.in silico     1   CCTGCGAACTTTGGTCTGCTGGAGTGCTTTGTTGTTCTCTTTGTTGTGCCTTTGCCTCGG 60


3044R-4.IR_full       61  GTTCATCGGCCTGGGATTACTCGGCATCCAAACTGGGGAAGTGCACAAGGTGGGGCAGCG 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3044R-4.in silico     61  GTTCATCGGCCTGGGATTACTCGGCATCCAAACTGGGGAAGTGCACAAGGTGGGGCAGCG 120


3044R-4.IR_full       121 ATTGGTGTGCTACTACGCCAGCGATGGCACCCACAATCTCAGCCTTTTGGATGTTCCCGG 180
                           ||||||||||||||||| |||||||||||||||||||||||||||||||||||||||||
3044R-4.in silico     121 GTTGGTGTGCTACTACGCAAGCGATGGCACCCACAATCTCAGCCTTTTGGATGTTCCCGG 180


3044R-4.IR_full       181 CGATCTGTGCACCCACATCAATATCGGACCCGCCACTTTGGATAATGCCACCATAGTCCT 240
                          ||| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3044R-4.in silico     181 CGACCTGTGCACCCACATCAATATCGGACCCGCCACTTTGGATAATGCCACCATAGTCCT 240


3044R-4.IR_full       241 TCCCGATACCCTCAGACAGGTACTACAGAACGACACCCGATCCTTTCGTGCTGCCCATCC 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3044R-4.in silico     241 TCCCGATACCCTCAGACAGGTACTACAGAACGACACCCGATCCTTTCGTGCTGCCCATCC 300


3044R-4.IR_full       301 GCAGGTTCACCTGCTTCTCTGGATCGGTGGCGCCGACAGTGGGCGGTCATTTGCCCTCAT 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3044R-4.in silico     301 GCAGGTTCACCTGCTTCTCTGGATCGGTGGCGCCGACAGTGGGCGGTCATTTGCCCTCAT 360


3044R-4.IR_full       361 GGTGGCGAACCACGCCATGCGAAAGTTGTTCCTGCGCTNCCTCCGGGAAATATTGCGCAC 420
                          |||||||||||||||||||||||||||||||||||||| |||||||||||||||||||||
3044R-4.in silico     361 GGTGGCGAACCACGCCATGCGAAAGTTGTTCCTGCGCTCCCTCCGGGAAATATTGCGCAC 420


3044R-4.IR_full       421 CTATCCCAGTTTGGATGGCATCGATCTCGACTGGGAGTTTCCGAGTGCCTACGATCGTGA 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3044R-4.in silico     421 CTATCCCAGTTTGGATGGCATCGATCTCGACTGGGAGTTTCCGAGTGCCTACGATCGTGA 480


3044R-4.IR_full       481 GCGAATGCATCTCTCGCAGC 500
                          ||||||||||||||||||||
3044R-4.in silico     481 GCGAATGCATCTCTCGCAGC 500

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	2	NM_132133.1	CG3044-RA (CG3044), mRNA
0.41	2	6	8	11	NM_142057.2	CG9307-RA (CG9307), mRNA
0.2	1	2	2	3	NM_079741.2	CG10210-RA (tst), mRNA
0	0	4	12	4	NM_137477.2	CG5154-RA (Idgf5), mRNA
0	0	3	4	11	NM_167080.1	CG32742-RA (l(1)G0148), mRNA
0	0	3	2	5	NM_142495.1	CG14299-RA, transcript variant A (CG14299), mRNA
0	0	3	2	5	NM_001014640.1	CG14299-RB, transcript variant B (CG14299), mRNA
0	0	2	7	3	NM_168574.1	CG6603-RC, transcript variant C (Hsc70Cb), mRNA
0	0	2	7	3	NM_168573.1	CG6603-RB, transcript variant B (Hsc70Cb), mRNA
0	0	2	7	3	NM_140430.2	CG6603-RA, transcript variant A (Hsc70Cb), mRNA
0	0	2	5	4	NM_057950.2	CG2054-RA (Cht2), mRNA
0	0	1	5	5	NM_078956.2	CG12210-RA, transcript variant A (Syb), mRNA
0	0	1	4	7	NM_079572.2	CG9484-RA (hyd), mRNA
0	0	1	3	4	NM_057754.2	CG9484-RA (hyd), mRNA, type I CG4099-RA (Sr-CI), mRNA
0	0	1	3	4	NM_168038.1	CG32264-RD, transcript variant D (CG32264), mRNA
0	0	1	3	3	NM_137871.2	CG3536-RA (CG3536), mRNA
0	0	1	3	3	NM_166551.2	CG30267-RA (CG30267), mRNA
0	0	1	3	2	NM_143318.2	CG31057-RA (tau), mRNA
0	0	0	18	20	NM_205954.2	CG33300-RA (CG33300), mRNA
0	0	0	8	55	NM_175960.3	CG33196-RB (dp), mRNA
0	0	0	7	11	NM_167207.1	CG1780-RB, transcript variant B (Idgf4), mRNA
0	0	0	7	11	NM_078546.2	CG1780-RA, transcript variant A (Idgf4), mRNA
0	0	0	6	3	NM_143058.1	CG13645-RA, transcript variant A (Nmnat), mRNA
0	0	0	6	3	NM_170185.1	CG13645-RB, transcript variant B (Nmnat), mRNA
0	0	0	5	3	NM_165411.1	CG6448-RA, transcript variant A (CG6448), mRNA
0	0	0	5	3	NM_136290.2	CG6448-RB, transcript variant B (CG6448), mRNA
0	0	0	5	2	NM_135451.1	CG3818-RA (CG3818), mRNA
0	0	0	4	8	NM_167325.1	CG11138-RB, transcript variant B (CG11138), mRNA
0	0	0	4	8	NM_132581.1	CG11138-RC, transcript variant C (CG11138), mRNA
0	0	0	4	8	NM_135028.2	CG15625-RA (CG15625), mRNA

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