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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
30394R-1 |
| Symbol |
CG30394 |
Full Name |
CG30394 |
| CG No |
CG30394 |
Old CG No |
CG30394 |
| Synonyms |
CG15670, CG10500, anon-WO0172774.188, anon-WO0172774.187, CG30394 |
| Accession No (Link to NCBI) |
NM_137735.1 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgCCACTCCGCGCATGTAATGAC |
| Primer Seq. 3' |
aatctagaggtaccGGGCCTCCAACACGATCTTGA |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 CCACTCCGCG CATGTAATGA CGTTGGCCAA CAGCATCATA GGCGTCGGCA TCTTGGCCAT
0061 GCCGTTCTGC TTCCAGAAGT GCGGCATCCT GTTATCCATC GTTCTTCTTG TGCTAAGCAA
0121 TGGAATAACC CGGGTTTGCT GCCACTATCT TATTAAAACC TCGCTGCTGA CACGTAGGAG
0181 GAGCTTCGAG ATGCTCGGTC TCCATGCTTT CGGTACTTCC GGCAAGCTGC TGGTGGAGCT
0241 GTGCATCATT GGCTACCTGA TTGGAACGTG CATCACGTAT TTTGTGGTCG TGGGCGATCT
0301 GGGTCCGCAG ATAATCGCCA AGATTTTCGC GCTGGACGTG GCAGATCACC TGCATCTGCG
0361 GAGTCTGGTC ATGGTCGTAG TCACTGTGGT GTGCATAGTG CCCCTCGGCA TGCTCNCGCA
0421 ATGTGGACAG CTTGTCCGCC GTTTGCACCG CGTCCATAGG TTTCTATGTC TGTCTAATTC
0481 TCAAGATCGT GTTGGAGGCC C |
| in silico PCR Fragment |
0001 CCACTCCGCG CATGTAATGA CGTTGGCCAA CAGCATCATA GGCGTCGGCA TCTTGGCCAT
0061 GCCGTTCTGC TTCCAGAAGT GCGGCATCCT GTTATCCATC GTTCTTCTTG TGCTAAGCAA
0121 TGGAATAACC CGGGTTTGCT GCCACTATCT TATTAAAACC TCGCTGCTGA CACGTAGGAG
0181 GAGCTTCGAG ATGCTCGGTC TCCATGCTTT CGGTACTTCC GGCAAGCTGC TGGTGGAGCT
0241 GTGCATCATT GGCTACCTGA TTGGAACGTG CATCACGTAT TTTGTGGTCG TGGGCGATCT
0301 GGGTCCGCAG ATAATCGCCA AGATTTTCGC GCTGGACGTG GCAGATCACC TGCATCTGCG
0361 GAGTCTGGTC ATGGTCGTAG TCACTGTGGT GTGCATAGTG CCCCTCGGCA TGCTC-CGCA
0421 ATGTGGACAG CTTGTCCGCC GTTTGCACCG CGTCCATAGG TTTCTATGTC TGCCTAATTC
0481 TCAAGATCGT GTTGGAGGCC C |
| Assemble Data |
30394R-1.IR_full 1 CCACTCCGCGCATGTAATGACGTTGGCCAACAGCATCATAGGCGTCGGCATCTTGGCCAT 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
30394R-1.in silico 1 CCACTCCGCGCATGTAATGACGTTGGCCAACAGCATCATAGGCGTCGGCATCTTGGCCAT 60
30394R-1.IR_full 61 GCCGTTCTGCTTCCAGAAGTGCGGCATCCTGTTATCCATCGTTCTTCTTGTGCTAAGCAA 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
30394R-1.in silico 61 GCCGTTCTGCTTCCAGAAGTGCGGCATCCTGTTATCCATCGTTCTTCTTGTGCTAAGCAA 120
30394R-1.IR_full 121 TGGAATAACCCGGGTTTGCTGCCACTATCTTATTAAAACCTCGCTGCTGACACGTAGGAG 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
30394R-1.in silico 121 TGGAATAACCCGGGTTTGCTGCCACTATCTTATTAAAACCTCGCTGCTGACACGTAGGAG 180
30394R-1.IR_full 181 GAGCTTCGAGATGCTCGGTCTCCATGCTTTCGGTACTTCCGGCAAGCTGCTGGTGGAGCT 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
30394R-1.in silico 181 GAGCTTCGAGATGCTCGGTCTCCATGCTTTCGGTACTTCCGGCAAGCTGCTGGTGGAGCT 240
30394R-1.IR_full 241 GTGCATCATTGGCTACCTGATTGGAACGTGCATCACGTATTTTGTGGTCGTGGGCGATCT 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
30394R-1.in silico 241 GTGCATCATTGGCTACCTGATTGGAACGTGCATCACGTATTTTGTGGTCGTGGGCGATCT 300
30394R-1.IR_full 301 GGGTCCGCAGATAATCGCCAAGATTTTCGCGCTGGACGTGGCAGATCACCTGCATCTGCG 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
30394R-1.in silico 301 GGGTCCGCAGATAATCGCCAAGATTTTCGCGCTGGACGTGGCAGATCACCTGCATCTGCG 360
30394R-1.IR_full 361 GAGTCTGGTCATGGTCGTAGTCACTGTGGTGTGCATAGTGCCCCTCGGCATGCTCNCGCA 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||| ||||
30394R-1.in silico 361 GAGTCTGGTCATGGTCGTAGTCACTGTGGTGTGCATAGTGCCCCTCGGCATGCTC-CGCA 420
30394R-1.IR_full 421 ATGTGGACAGCTTGTCCGCCGTTTGCACCGCGTCCATAGGTTTCTATGTCTGTCTAATTC 480
|||||||||||||||||||||||||||||||||||||||||||||||||||| |||||||
30394R-1.in silico 421 ATGTGGACAGCTTGTCCGCCGTTTGCACCGCGTCCATAGGTTTCTATGTCTGCCTAATTC 480
30394R-1.IR_full 481 TCAAGATCGTGTTGGAGGCCC 501
|||||||||||||||||||||
30394R-1.in silico 481 TCAAGATCGTGTTGGAGGCCC 501
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_137735.1 |
CG30394-RA, transcript variant A (CG30394), mRNA |
| 100 |
482 |
0 |
0 |
0 |
NM_137734.2 |
CG30394-RB, transcript variant B (CG30394), mRNA |
| 0 |
0 |
3 |
5 |
3 |
NM_168779.1 |
CG3979-RC, transcript variant C (Indy), mRNA |
| 0 |
0 |
3 |
5 |
3 |
NM_168778.1 |
CG3979-RB, transcript variant B (Indy), mRNA |
| 0 |
0 |
3 |
5 |
3 |
NM_079426.2 |
CG3979-RA, transcript variant A (Indy), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_135735.1 |
CG5525-RA (CG5525), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_137616.2 |
CG11208-RA (CG11208), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_133027.1 |
CG12985-RA (CG12985), mRNA |
| 0 |
0 |
1 |
5 |
11 |
NM_169143.1 |
CG10272-RD, transcript variant D (gpp), mRNA |
| 0 |
0 |
1 |
5 |
11 |
NM_141398.1 |
CG10272-RA, transcript variant A (gpp), mRNA |
| 0 |
0 |
1 |
5 |
11 |
NM_169142.1 |
CG10272-RB, transcript variant B (gpp), mRNA |
| 0 |
0 |
1 |
4 |
6 |
NM_169144.1 |
CG10272-RC, transcript variant C (gpp), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_206441.1 |
CG31481-RB, transcript variant B (pb), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_057321.4 |
CG31481-RA, transcript variant A (pb), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_206439.1 |
CG31481-RD, transcript variant D (pb), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_206440.1 |
CG31481-RC, transcript variant C (pb), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_140936.2 |
CG17147-RA (CG17147), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_001043097.1 |
CG9854-RC, transcript variant C (hrg), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_166372.1 |
CG9854-RB, transcript variant B (hrg), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_080529.2 |
CG9854-RA, transcript variant A (hrg), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_136812.1 |
CG13223-RA (CG13223), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_134846.2 |
CG3227-RA (insv), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_141219.1 |
CG1078-RA (CG1078), mRNA |
| 0 |
0 |
0 |
7 |
5 |
NM_001014526.1 |
CG33528-RE, transcript variant E (CG33528), mRNA |
| 0 |
0 |
0 |
7 |
5 |
NM_001014524.1 |
CG33528-RC, transcript variant C (CG33528), mRNA |
| 0 |
0 |
0 |
7 |
5 |
NM_001014525.1 |
CG33528-RD, transcript variant D (CG33528), mRNA |
| 0 |
0 |
0 |
5 |
13 |
NM_134512.4 |
CG12701-RA, transcript variant A (CG12701), mRNA |
| 0 |
0 |
0 |
5 |
13 |
NM_167685.1 |
CG12701-RB, transcript variant B (CG12701), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_142750.1 |
CG13408-RA (CG13408), mRNA |
| 0 |
0 |
0 |
4 |
8 |
NM_166546.2 |
CG30092-RD, transcript variant D (jbug), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
JNK signal
| N |
 |
| +- |
|
| DB |
 |
| +- |
|
| DC |
 |
| ++ |
|
| DM |
 |
| +- |
|
| VM |
 |
| |
|
| VB |
 |
| |
|
|
Caspase-3 signal
| N |
|
| + |
|
| DB |
|
| ++ |
|
| DC |
|
| ++ |
|
| DM |
|
| + |
|
| VM |
|
| + |
|
| VB |
|
| |
|
|
| Dcompartment |
|
Penetrance |
| + |
|
|
|
|
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