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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 30386R-3 
 Symbol CG30386  Full Name CG30386 
 CG No CG30386  Old CG No CG30386 
 Synonyms CG30386 
 Accession No (Link to NCBI) NM_166440.1 
 Inserted Chr. ll 
 Insertional Mutation  semi-llethal 
 Phenotype induced by Act5C-GAL4 at 28 degrees semi-lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgCAAAAGAGGGCGTCCATCGCA 
 Primer Seq. 3' 
aatctagaggtaccTCCAACAAGCGGTAGAGCAAC 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 CAAAAGAGGG CGTCCATCGC AGAGGAAACT CCTGTCTCCG ATTCCACAAC TCCAGCCACA 
0061 ACAGCATCAC CCAGCTCACC TAGCAGAACT GCTGGCCACT CCACCATCGG TGCTTTGGTC 
0121 AGTAACCTGC ACCACCATGA CATTCAGTCC CTGCATCAAC CACTGCTGGA GAGCGAGGAG 
0181 CGGGTGATAG TCGCGGTTAA CCACAGGAAT CCTCGCCAGC CACATCGGAG TTATGGGACC 
0241 GAGCGGAAGC CGCACAGTTC CCTGCCCCGT TTCGTGATCA ACATTGAGGA GGAGACGGAT 
0301 GCAGCGGTGG CTGCGGCTGA GGGAAAGAGC AGTCCCGCTC CTAACCAGGA GCACGAGGAG 
0361 CACTCCAACC AGAGTCCTTC CCGCCGTTTC AGTCACTTCA ATCTGGCCCT GCGTCGCTTC 
0421 TCCCACATCC ATGCCCACGT AAATGCCGAT TATCCTGGCT ATGTTGGTGT TGTAACCTTG 
0481 TTGCTCTACC GCTTGTTGGA  
 in silico PCR Fragment 
0001 CAAAAGAGGG CGTCCATCGC AGAGGAAACT CCTGTCTCCG ATTCCACAAC TCCAGCCACA 
0061 ACAGCATCAC CCAGCTCACC CAGCAGAACT GCTGGCCACT CCACCATCGG TGCTTTGGTC 
0121 AGCAACCTGC ACCACCATGA CATTCAGTCC CTGCATCAAC CACTGCTGGA GAGCGAGGAG 
0181 CGGGTGATAG TCGCGGTTAA CCACAGGAAT CCTCGCCAGC CACATCGGAG TTATGGGACC 
0241 GAGCGGAAGC CGCACAGTTC CCTGCCCCGT TTCGTGATCA ACATTGAGGA GGAGACGGAT 
0301 GCAGCGGTGG CTGCGGCTGA GGGAAAGAGC AGTCCCGCTC CTAACCAGGA GCACGAGGAG 
0361 CACTCCAACC AGAGTCCTTC CCGCCGTTTC AGTCACTTCA ATCTGGCCCT GCGTCGCTTC 
0421 TCCCACATCC ATGCCCACGT AAATGCCGAT TATCCTGGCT ATGTTGGTGT TGTAACCTTG 
0481 TTGCTCTACC GCTTGTTGGA  
 Assemble Data 

30386R-3.IR_full       1   CAAAAGAGGGCGTCCATCGCAGAGGAAACTCCTGTCTCCGATTCCACAACTCCAGCCACA 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
30386R-3.in silico     1   CAAAAGAGGGCGTCCATCGCAGAGGAAACTCCTGTCTCCGATTCCACAACTCCAGCCACA 60


30386R-3.IR_full       61  ACAGCATCACCCAGCTCACCTAGCAGAACTGCTGGCCACTCCACCATCGGTGCTTTGGTC 120
                           |||||||||||||||||||| |||||||||||||||||||||||||||||||||||||||
30386R-3.in silico     61  ACAGCATCACCCAGCTCACCCAGCAGAACTGCTGGCCACTCCACCATCGGTGCTTTGGTC 120


30386R-3.IR_full       121 AGTAACCTGCACCACCATGACATTCAGTCCCTGCATCAACCACTGCTGGAGAGCGAGGAG 180
                           || |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
30386R-3.in silico     121 AGCAACCTGCACCACCATGACATTCAGTCCCTGCATCAACCACTGCTGGAGAGCGAGGAG 180


30386R-3.IR_full       181 CGGGTGATAGTCGCGGTTAACCACAGGAATCCTCGCCAGCCACATCGGAGTTATGGGACC 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
30386R-3.in silico     181 CGGGTGATAGTCGCGGTTAACCACAGGAATCCTCGCCAGCCACATCGGAGTTATGGGACC 240


30386R-3.IR_full       241 GAGCGGAAGCCGCACAGTTCCCTGCCCCGTTTCGTGATCAACATTGAGGAGGAGACGGAT 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
30386R-3.in silico     241 GAGCGGAAGCCGCACAGTTCCCTGCCCCGTTTCGTGATCAACATTGAGGAGGAGACGGAT 300


30386R-3.IR_full       301 GCAGCGGTGGCTGCGGCTGAGGGAAAGAGCAGTCCCGCTCCTAACCAGGAGCACGAGGAG 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
30386R-3.in silico     301 GCAGCGGTGGCTGCGGCTGAGGGAAAGAGCAGTCCCGCTCCTAACCAGGAGCACGAGGAG 360


30386R-3.IR_full       361 CACTCCAACCAGAGTCCTTCCCGCCGTTTCAGTCACTTCAATCTGGCCCTGCGTCGCTTC 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
30386R-3.in silico     361 CACTCCAACCAGAGTCCTTCCCGCCGTTTCAGTCACTTCAATCTGGCCCTGCGTCGCTTC 420


30386R-3.IR_full       421 TCCCACATCCATGCCCACGTAAATGCCGATTATCCTGGCTATGTTGGTGTTGTAACCTTG 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
30386R-3.in silico     421 TCCCACATCCATGCCCACGTAAATGCCGATTATCCTGGCTATGTTGGTGTTGTAACCTTG 480


30386R-3.IR_full       481 TTGCTCTACCGCTTGTTGGA 500
                           ||||||||||||||||||||
30386R-3.in silico     481 TTGCTCTACCGCTTGTTGGA 500



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_166440.1  CG30386-RA (CG30386), mRNA 
0.2   1  2  5  4  NM_141661.1  CG8312-RA, transcript variant A (CG8312), mRNA 
0   0  4  5  9  NM_143615.2  CG11518-RA (pygo), mRNA 
0   0  4  2  2  NM_143211.2  CG6073-RA (CG6073), mRNA 
0   0  3  7  4  NM_132878.1  CG9216-RA, transcript variant A (CG9216), mRNA 
0   0  3  4  2  NM_167486.1  CG9216-RB, transcript variant B (CG9216), mRNA 
0   0  3  4  2  NM_167485.1  CG9216-RC, transcript variant C (CG9216), mRNA 
0   0  3  3  14  NM_078592.2  CG11172-RA (NFAT), mRNA 
0   0  2  6  3  NM_167228.1  CG32681-RA (CG32681), mRNA 
0   0  2  3  5  NM_136202.4  CG31678-RA (CG31678), mRNA 
0   0  2  3  4  NM_141795.2  CG6693-RA (CG6693), mRNA 
0   0  2  3  3  NM_176374.1  CG4761-RA (knrl), mRNA 
0   0  2  2  4  NM_137447.2  CG5098-RA (CG5098), mRNA 
0   0  1  5  5  NM_130715.2  CG13316-RB, transcript variant B (Mnt), mRNA 
0   0  1  5  5  NM_166983.1  CG13316-RA, transcript variant A (Mnt), mRNA 
0   0  1  5  5  NM_166984.1  CG13316-RC, transcript variant C (Mnt), mRNA 
0   0  1  4  10  NM_168195.1  CG32387-RB, transcript variant B (CG32387), mRNA 
0   0  1  4  10  NM_001043123.1  CG32387-RC, transcript variant C (CG32387), mRNA 
0   0  1  4  9  NM_169258.1  CG9755-RA, transcript variant A (pum), mRNA 
0   0  1  4  9  NM_169259.1  CG9755-RD, transcript variant D (pum), mRNA 
0   0  1  4  9  NM_079561.2  CG9755-RC, transcript variant C (pum), mRNA 
0   0  1  4  8  NM_169177.1  CG2507-RB, transcript variant B (sas), mRNA 
0   0  1  4  8  NM_168196.1  CG32387-RA, transcript variant A (CG32387), mRNA 
0   0  1  4  8  NM_165794.2  CG11895-RA (stan), mRNA 
0   0  1  4  7  NM_057263.2  CG2507-RA, transcript variant A (sas), mRNA 
0   0  1  4  6  NM_169260.1  CG9755-RB, transcript variant B (pum), mRNA 
0   0  1  4  4  NM_001038921.1  CG33991-RD, transcript variant D (nuf), mRNA 
0   0  1  4  4  NM_001038917.1  CG33991-RF, transcript variant F (nuf), mRNA 
0   0  1  4  4  NM_001038919.1  CG33991-RE, transcript variant E (nuf), mRNA 
0   0  1  4  4  NM_001038920.1  CG33991-RC, transcript variant C (nuf), mRNA 

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