NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 3033R-2

Symbol CG3033 Full Name CG3033

CG No CG3033 Old CG No CG3033

Synonyms CG3033

Accession No (Link to NCBI) NM_132045.2

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees larval lethal

Map Viewer

Reference
[Please submit your publication] Yano H, Yamamoto-Hino M, Awano W, Aoki-Kinoshita KF, Tsuda-Sakurai K, Okano H, Goto S.
Identification of proteasome components required for apical localization of Chaoptin using functional genomics.
J Neurogenet (2012) 26(1) 53-63 [ PubMed ID = 22417167 ] [ RRC reference ]

Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624 [ PubMed ID = 21666802 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCGATATCGACGCAAAGCAAGC

Primer Seq. 3'

aatctagaggtaccAAAATCGGCGAAGGCCAGCAG

Predicted Fragment Size

IR fragment full Seq

0001 CGATATTCGA CGCAAAGCAA GCTGGTGGAC GGACTGGCGC GTCATGTTCG CAAGGTGTGC 
0061 TACGCCCTTT ATGTGGCGGG TGTGGCCTGG TTCTTCTGTT TGGCGCTGCC GGAGTTCAAC 
0121 CACGGGACAT ATCTCTCCGA GAATGCTCTT TCGCCAGGCT TGGTCTATCC GGAGATCCGC 
0181 ATCGATGCGA ATCGTCTGGC CATCCAGTTG CTGGAGGAGC TGCAGCGGGA GCGAAAGGAT 
0241 CATCTGTCCA CCACGCCGCA CGCCTGGATT GCGGCCAAGA TGAACGAGTT CGGCCTAGAG 
0301 ACGCANCACG CACAACTACA CGTTGCGCTA CCCATTTGGC GGCGGTAAAG AGTATCACGG 
0361 CAAGAATATC TACGGCATAT TGAGGGCCCC AAGAATTGNC TCCACGGAGG GCATCGTTTT 
0421 TGCGGCTCCA TATCGCGCTG CCAGTTCCGT GCACACGGAC ATTTCCGNCC AGCGTGCCGC 
0481 TTCTGCTGGC CTTCGCCGAT TTT

in silico PCR Fragment

0001 CGATA-TCGA CGCAAAGCAA GCTGGTGGAC GGACTGGCGC GTCATGTTCG CAAGGTGTGC 
0061 TACGCCCTTT ATGTGGCGGG TGTGGCCTGG TTCTTCTGTT TGGCGCTGCC GGAGTTCAAC 
0121 CACGGGACAT ATCTCTCCGA GAATGCTCTT TCGCCAGGCT TGGTCTATCC GGAGATCCGC 
0181 ATCGATGCGA ATCGTCTGGC CATCCAGTTG CTGGAGGAGC TGCAGCGGGA GCGAAAGGAT 
0241 CATCTGTCCA CCACGCCGCA CGCCTGGATT GCGGCCAAGA TGAACGAGTT CGGCCTGGAG 
0301 ACGCA-CACG CACAACTACA CGTTGCGCTA CCCATTTGGC GGCGGTAAAG AGTATCACGG 
0361 CAAGAATATC TACGGCATAT TGAGGGCCCC AAGAATTGCC TCCACGGAGG GCATCGTTTT 
0421 TGCGGCTCCA TATCGCGCTG CCAGTTCCGT GCACACGGAC ATTTCCG-CC AGCGTGCCGC 
0481 TTCTGCTGGC CTTCGCCGAT TTT

Assemble Data


3033R-2.IR_full       1   CGATATTCGACGCAAAGCAAGCTGGTGGACGGACTGGCGCGTCATGTTCGCAAGGTGTGC 60
                          ||||| ||||||||||||||||||||||||||||||||||||||||||||||||||||||
3033R-2.in silico     1   CGATA-TCGACGCAAAGCAAGCTGGTGGACGGACTGGCGCGTCATGTTCGCAAGGTGTGC 60


3033R-2.IR_full       61  TACGCCCTTTATGTGGCGGGTGTGGCCTGGTTCTTCTGTTTGGCGCTGCCGGAGTTCAAC 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3033R-2.in silico     61  TACGCCCTTTATGTGGCGGGTGTGGCCTGGTTCTTCTGTTTGGCGCTGCCGGAGTTCAAC 120


3033R-2.IR_full       121 CACGGGACATATCTCTCCGAGAATGCTCTTTCGCCAGGCTTGGTCTATCCGGAGATCCGC 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3033R-2.in silico     121 CACGGGACATATCTCTCCGAGAATGCTCTTTCGCCAGGCTTGGTCTATCCGGAGATCCGC 180


3033R-2.IR_full       181 ATCGATGCGAATCGTCTGGCCATCCAGTTGCTGGAGGAGCTGCAGCGGGAGCGAAAGGAT 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3033R-2.in silico     181 ATCGATGCGAATCGTCTGGCCATCCAGTTGCTGGAGGAGCTGCAGCGGGAGCGAAAGGAT 240


3033R-2.IR_full       241 CATCTGTCCACCACGCCGCACGCCTGGATTGCGGCCAAGATGAACGAGTTCGGCCTAGAG 300
                          |||||||||||||||||||||||||||||||||||||||||||||||||||||||| |||
3033R-2.in silico     241 CATCTGTCCACCACGCCGCACGCCTGGATTGCGGCCAAGATGAACGAGTTCGGCCTGGAG 300


3033R-2.IR_full       301 ACGCANCACGCACAACTACACGTTGCGCTACCCATTTGGCGGCGGTAAAGAGTATCACGG 360
                          ||||| ||||||||||||||||||||||||||||||||||||||||||||||||||||||
3033R-2.in silico     301 ACGCA-CACGCACAACTACACGTTGCGCTACCCATTTGGCGGCGGTAAAGAGTATCACGG 360


3033R-2.IR_full       361 CAAGAATATCTACGGCATATTGAGGGCCCCAAGAATTGNCTCCACGGAGGGCATCGTTTT 420
                          |||||||||||||||||||||||||||||||||||||| |||||||||||||||||||||
3033R-2.in silico     361 CAAGAATATCTACGGCATATTGAGGGCCCCAAGAATTGCCTCCACGGAGGGCATCGTTTT 420


3033R-2.IR_full       421 TGCGGCTCCATATCGCGCTGCCAGTTCCGTGCACACGGACATTTCCGNCCAGCGTGCCGC 480
                          ||||||||||||||||||||||||||||||||||||||||||||||| ||||||||||||
3033R-2.in silico     421 TGCGGCTCCATATCGCGCTGCCAGTTCCGTGCACACGGACATTTCCG-CCAGCGTGCCGC 480


3033R-2.IR_full       481 TTCTGCTGGCCTTCGCCGATTTT 503
                          |||||||||||||||||||||||
3033R-2.in silico     481 TTCTGCTGGCCTTCGCCGATTTT 503

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	2	4	NM_132045.2	CG3033-RA (CG3033), mRNA
0.2	1	4	2	4	NM_078924.3	CG12165-RA (Incenp), mRNA
0.2	1	2	2	5	NM_135010.1	CG11928-RA (CG11928), mRNA
0	0	5	8	6	NM_139934.3	CG8006-RA (CG8006), mRNA
0	0	3	5	3	NM_132132.1	CG4557-RA (CG4557), mRNA
0	0	3	2	4	NM_167160.1	CG1634-RB, transcript variant B (Nrg), mRNA
0	0	2	4	11	NM_078517.2	CG2175-RA, transcript variant A (dec-1), mRNA
0	0	2	4	11	NM_167133.1	CG2175-RC, transcript variant C (dec-1), mRNA
0	0	2	4	11	NM_167132.1	CG2175-RB, transcript variant B (dec-1), mRNA
0	0	2	3	6	NM_144343.2	CG8250-RA (Alk), mRNA
0	0	2	3	3	NM_080356.2	CG5993-RA (os), mRNA
0	0	2	2	6	NM_169455.1	CG4795-RB, transcript variant B (Cpn), mRNA
0	0	2	2	6	NM_169454.1	CG4795-RA, transcript variant A (Cpn), mRNA
0	0	2	2	3	NM_164692.2	CG31638-RA (CG31638), mRNA
0	0	1	6	3	NM_079105.2	CG4084-RA (l(2)not), mRNA
0	0	1	5	4	NM_168837.1	CG17233-RC, transcript variant C (CG17233), mRNA
0	0	1	5	4	NM_140939.2	CG17233-RB, transcript variant B (CG17233), mRNA
0	0	1	4	18	NM_134544.1	CG11734-RB (HERC2), mRNA
0	0	1	4	9	NM_079903.2	CG15319-RB (nej), mRNA
0	0	1	4	6	NM_167188.1	CG32703-RA (CG32703), mRNA
0	0	1	4	6	NM_132323.1	CG17438-RA (CG17438), mRNA
0	0	1	4	4	NM_165611.1	CG8643-RA (rgr), mRNA
0	0	1	4	2	NM_137920.1	CG9899-RA (CG9899), mRNA
0	0	1	3	8	NM_141446.1	CG10055-RA (CG10055), mRNA
0	0	1	3	4	NM_139721.1	CG10591-RA (CG10591), mRNA
0	0	1	3	2	NM_139792.2	CG10125-RA (zpg), mRNA
0	0	1	2	3	NM_079311.2	CG5638-RA (Rh7), mRNA
0	0	1	2	3	NM_167109.1	CG4607-RB, transcript variant B (CG4607), mRNA
0	0	1	2	3	NM_135982.2	CG6304-RA (CG6304), mRNA
0	0	1	2	3	NM_132152.1	CG4607-RA, transcript variant A (CG4607), mRNA

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