National Institute of Genetics
Contact us      Quick Search       
NIG-Fly - Fly Stocks of National Institute of Genetics (NIG) Drosophila strain -NIG-Fly - Fly Stocks of National Institute of Genetics (NIG) Drosophila strain -NIG-Fly - Fly Stocks of National Institute of Genetics (NIG) Drosophila strain -
Home Home
Japanese | English  
Notice:

Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
NIG-RNAi Strains Detail
Request : Click the "Order" button to request for this stock discarded request_help.gif
Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 30284R-6 
 Symbol CG30284  Full Name CG30284 
 CG No CG30284  Old CG No CG30284 
 Synonyms NEST:bs16b04, BcDNA:AT12437, BcDNA:AT22714, CG30284 
 Accession No (Link to NCBI) NM_166468.2 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgCTGCAGGAGCCCGGCCATAAT 
 Primer Seq. 3' 
aatctagaggtaccTGTTGAACAGTGCGTCCACCC 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 NTGCAgGAGC CCGGCCATAA TACGCAGtCC GCTGAACTCT GAGGAATTTC AGGACTCGGC 
0061 AACGGCAGcC NCCACACAAC AACGGCTTTT CTGGAGCGGC CAgNGNGCNT CCGACGGCGG 
0121 GCAGGGATGA TAAAACCAAG CCTGATTCGA CTGCCGCCGG AAAAAAGAAG CCACCTACGA 
0181 CGACAAGGCA GTCAAATTTT ACCCAGACCG AACGAAGACG GACACATCGC AAGCTGAGTC 
0241 CGCGGAGCAC GCGTTGCAAT TCGGTGGCAG TTACGGCGCT GGTCAGCAGA GAAAATATAC 
0301 CGCAAACATC CGGTAAGCGC GGAAAAAAAA CTCCGAGCCA CCGGAGCACA CTAATAAGTA 
0361 CTCCATCGAA AACACTACAA TTAAAAACGG CCGCAGGGTA CAGAAGGCCA GAGGACCTAA 
0421 CCGCCCGGTG CANTgCGTCG CACGAGGGGC GCAAGCTTCT GCAGGAGCGG CTGATGGTGC 
0481 GGGTGGACGC ACTGTTCAAC A 
 in silico PCR Fragment 
0001 CTGCAGGAGC CCGGCCATAA TACGCAGTCC GCTGAACTCT GAGGAATTTC AGGACTCGGC 
0061 AACGGCAGCC GCCACACAAC AACGGCTTTT CTGGAGCGGC CAGCGGGCCT -CGACGGCGG 
0121 GCAGGGATGA TAAAACCAAG CCTGATTCGA CTGCCGCCGG AAAAAAGAAG CCACCTACGA 
0181 CGACAAGGCA GTCAAATTTT ACCCAGACCG AACGAAGACG GACACATCGC AAGCTGAGTC 
0241 CGCGGAGCAC GCGTTGCAAT TCGGTGGCAG TTACGGCGCT GGTCAGCAGA GAAAATATAC 
0301 CGCAAACATC CGGTAAGCGC GGAAAAAAAA CTCCGAGCCA CCGGAGCACA CTAATAAGTA 
0361 CTCCATCGAA AACACTACAA TTAAAAACGG CCGCAGGGTA CAGAAGGCCA GAGGACCTAA 
0421 CCGCCCGGTG CAGTGCGTCG CACGAGGGGC GCAAGCTTCT GCAGGAGCGG CTGATGGTGC 
0481 GGGTGGACGC ACTGTTCAAC A 
 Assemble Data 

30284R-6.IR_full        1   TGCAGGAGCCCGGCCATAATACGCAGTCCGCTGAACTCTGAGGAATTTCAGGACTCGGC 59
                            |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
30284R-6.in silico      1   TGCAGGAGCCCGGCCATAATACGCAGTCCGCTGAACTCTGAGGAATTTCAGGACTCGGC 59


30284R-6.IR_full       61  AACGGCAGCCNCCACACAACAACGGCTTTTCTGGAGCGGCCAGNGNGCNTCCGACGGCGG 119
                           |||||||||| |||||||||||||||||||||||||||||||| | || | |||||||||
30284R-6.in silico     61  AACGGCAGCCGCCACACAACAACGGCTTTTCTGGAGCGGCCAGCGGGCCT-CGACGGCGG 119


30284R-6.IR_full       121 GCAGGGATGATAAAACCAAGCCTGATTCGACTGCCGCCGGAAAAAAGAAGCCACCTACGA 179
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
30284R-6.in silico     121 GCAGGGATGATAAAACCAAGCCTGATTCGACTGCCGCCGGAAAAAAGAAGCCACCTACGA 179


30284R-6.IR_full       181 CGACAAGGCAGTCAAATTTTACCCAGACCGAACGAAGACGGACACATCGCAAGCTGAGTC 239
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
30284R-6.in silico     181 CGACAAGGCAGTCAAATTTTACCCAGACCGAACGAAGACGGACACATCGCAAGCTGAGTC 239


30284R-6.IR_full       241 CGCGGAGCACGCGTTGCAATTCGGTGGCAGTTACGGCGCTGGTCAGCAGAGAAAATATAC 299
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
30284R-6.in silico     241 CGCGGAGCACGCGTTGCAATTCGGTGGCAGTTACGGCGCTGGTCAGCAGAGAAAATATAC 299


30284R-6.IR_full       301 CGCAAACATCCGGTAAGCGCGGAAAAAAAACTCCGAGCCACCGGAGCACACTAATAAGTA 359
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
30284R-6.in silico     301 CGCAAACATCCGGTAAGCGCGGAAAAAAAACTCCGAGCCACCGGAGCACACTAATAAGTA 359


30284R-6.IR_full       361 CTCCATCGAAAACACTACAATTAAAAACGGCCGCAGGGTACAGAAGGCCAGAGGACCTAA 419
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
30284R-6.in silico     361 CTCCATCGAAAACACTACAATTAAAAACGGCCGCAGGGTACAGAAGGCCAGAGGACCTAA 419


30284R-6.IR_full       421 CCGCCCGGTGCANTGCGTCGCACGAGGGGCGCAAGCTTCTGCAGGAGCGGCTGATGGTGC 479
                           |||||||||||| |||||||||||||||||||||||||||||||||||||||||||||||
30284R-6.in silico     421 CCGCCCGGTGCAGTGCGTCGCACGAGGGGCGCAAGCTTCTGCAGGAGCGGCTGATGGTGC 479


30284R-6.IR_full       481 GGGTGGACGCACTGTTCAACA 500
                           |||||||||||||||||||||
30284R-6.in silico     481 GGGTGGACGCACTGTTCAACA 500



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   398  0  0  1  NM_166467.2  CG30284-RB, transcript variant B (CG30284), mRNA 
95.97   382  3  3  4  NM_166468.2  CG30284-RA, transcript variant A (CG30284), mRNA 
0   0  3  3  4  NM_133159.1  CG14200-RA (CG14200), mRNA 
0   0  2  3  5  NM_079051.2  CG4903-RA (MESR4), mRNA 
0   0  1  4  2  NM_057532.3  CG6235-RA, transcript variant A (tws), mRNA 
0   0  1  4  2  NM_169329.1  CG6235-RF, transcript variant F (tws), mRNA 
0   0  1  4  2  NM_134286.1  CG6235-RB, transcript variant B (tws), mRNA 
0   0  1  4  2  NM_057533.2  CG6235-RE, transcript variant E (tws), mRNA 
0   0  1  4  2  NM_134284.1  CG6235-RD, transcript variant D (tws), mRNA 
0   0  1  4  2  NM_134285.1  CG6235-RC, transcript variant C (tws), mRNA 
0   0  1  4  2  NM_169330.1  CG6235-RG, transcript variant G (tws), mRNA 
0   0  1  4  2  NM_169331.1  CG6235-RH, transcript variant H (tws), mRNA 
0   0  1  3  10  NM_169489.1  CG7518-RA, transcript variant A (CG7518), mRNA 
0   0  1  3  10  NM_141993.2  CG7518-RB, transcript variant B (CG7518), mRNA 
0   0  1  3  3  NM_169530.1  CG9412-RC, transcript variant C (rin), mRNA 
0   0  1  3  3  NM_169532.1  CG9412-RE, transcript variant E (rin), mRNA 
0   0  1  3  3  NM_169533.1  CG9412-RA, transcript variant A (rin), mRNA 
0   0  1  3  3  NM_080168.2  CG9412-RB, transcript variant B (rin), mRNA 
0   0  1  3  3  NM_169531.1  CG9412-RD, transcript variant D (rin), mRNA 
0   0  1  3  2  NM_079485.2  CG7464-RA (CS-2), mRNA 
0   0  1  2  4  NM_132836.1  CG8206-RA (CG8206), mRNA 
0   0  1  2  3  NM_079492.2  CG11401-RA (Trxr-2), mRNA 
0   0  0  6  5  NM_167436.1  CG6170-RB, transcript variant B (HDAC6), mRNA 
0   0  0  6  5  NM_167437.1  CG6170-RC, transcript variant C (HDAC6), mRNA 
0   0  0  6  5  NM_132789.2  CG6170-RA, transcript variant A (HDAC6), mRNA 
0   0  0  6  5  NM_170184.1  CG6238-RB, transcript variant B (ssh), mRNA 
0   0  0  6  5  NM_079768.2  CG6238-RA, transcript variant A (ssh), mRNA 
0   0  0  5  7  NM_176229.1  CG33147-RA (CG33147), mRNA 
0   0  0  5  6  NM_134512.4  CG12701-RA, transcript variant A (CG12701), mRNA 
0   0  0  5  6  NM_167685.1  CG12701-RB, transcript variant B (CG12701), mRNA 

SHIGEN - SHared Information of GENetic resources - National Institute of Genetics (NIG)
 Copyright (c) National Institute of Genetics (NIG)
All rights reserved.
The NIG-Fly is a part of the National BioResource Project which started in 2002.