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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 30274R-9 
 Symbol CG30274  Full Name CG30274 
 CG No CG30274  Old CG No CG30274 
 Synonyms CG13529, CG30274 
 Accession No (Link to NCBI) NM_166563.1 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgTATTCGTTCCCGACGATCTGC 
 Primer Seq. 3' 
aatctagaggtaccGAAGACGAAAGCCGCTGTGTT 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 TATTCGTTCC CGACGATCTG CACAGTGCCC GACGGGAGCT GCAGGATGGC GAGATCTCAA 
0061 GGACCTTGGA GCGCTGCTTT AGCGTCATTA CGGAATCGGG TAAATTGCTG AGCGGCACTA 
0121 CGGCATCGGC NTCTATCGCA TCGAAAGCCA CTGAGTGCAT CCTCGCTGCA GCNTTTCGGT 
0181 GACCAGCTAT CTGAGTAGAT TCCTCAAACG TGGCGTCTAC GACAAGATCA AGCGCCGACA 
0241 GACCCGACTC GATCACAATC TCTTCGATGT CCTGTGGCCA GCGATGCGGA AAACATCGAN 
0301 AGGCGCGCCA CTTGGAGGAG GACATCAACT GCGGGATTAT AGCTCCAGAT TTCGATGTGT 
0361 TTGTGGTGTT CCAAGAGTTC TTGGTGCCGT TGCTTAAGGA CATGCATTGC CTGAGTATCG 
0421 ATGCTGATTT CAAACCGCAA CCTCGACTGG CCTATTTTCC CATGGACAAT GGCGAAAGGC 
0481 TCAACACAGC GGCTTTCGTC TTC 
 in silico PCR Fragment 
0001 TATTCGTTCC CGACGATCTG CACAGTGCCC GACGGGAGCT GCAGGATGGC GAGATCTCAA 
0061 GGACCTTGGA GCGCTGCTTT AGCGTCATTA CGGAATCGGG TAAATTGCTG AGCGGCACTA 
0121 CGGCATCGGC -TCTATCGCA TCGAAAGCCA CTGAGTGCAT CCTCGCTGCA GC-TTTCGGT 
0181 GACCAGCTAT CTGAGTAGAT TCCTCAAACG TGGCGTCTAC GACAAGATCA AGCGCCGACA 
0241 GACCCGACTC GATCACAATC TCTTCGATGT CCTGTGGCCA GCGATGCGGA AAACATCGA- 
0301 AGGCGCGCCA CTTGGAGGAG GACATCAACT GCGGGATTAT AGCTCCAGAT TTCGATGTGT 
0361 TTGTGGTGTT CCAAGAGTTC TTGGTGCCGT TGCTTAAGGA CATGCATTGC CTGAGTATCG 
0421 ATGCTGATTT CAAACCGCAA CCTCGACTGG CCTATTTTCC CATGGACAAT GGCGAAAGGC 
0481 TCAACACAGC GGCTTTCGTC TTC 
 Assemble Data 

30274R-9.IR_full       1   TATTCGTTCCCGACGATCTGCACAGTGCCCGACGGGAGCTGCAGGATGGCGAGATCTCAA 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
30274R-9.in silico     1   TATTCGTTCCCGACGATCTGCACAGTGCCCGACGGGAGCTGCAGGATGGCGAGATCTCAA 60


30274R-9.IR_full       61  GGACCTTGGAGCGCTGCTTTAGCGTCATTACGGAATCGGGTAAATTGCTGAGCGGCACTA 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
30274R-9.in silico     61  GGACCTTGGAGCGCTGCTTTAGCGTCATTACGGAATCGGGTAAATTGCTGAGCGGCACTA 120


30274R-9.IR_full       121 CGGCATCGGCNTCTATCGCATCGAAAGCCACTGAGTGCATCCTCGCTGCAGCNTTTCGGT 180
                           |||||||||| ||||||||||||||||||||||||||||||||||||||||| |||||||
30274R-9.in silico     121 CGGCATCGGC-TCTATCGCATCGAAAGCCACTGAGTGCATCCTCGCTGCAGC-TTTCGGT 180


30274R-9.IR_full       181 GACCAGCTATCTGAGTAGATTCCTCAAACGTGGCGTCTACGACAAGATCAAGCGCCGACA 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
30274R-9.in silico     181 GACCAGCTATCTGAGTAGATTCCTCAAACGTGGCGTCTACGACAAGATCAAGCGCCGACA 240


30274R-9.IR_full       241 GACCCGACTCGATCACAATCTCTTCGATGTCCTGTGGCCAGCGATGCGGAAAACATCGAN 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| 
30274R-9.in silico     241 GACCCGACTCGATCACAATCTCTTCGATGTCCTGTGGCCAGCGATGCGGAAAACATCGA- 300


30274R-9.IR_full       301 AGGCGCGCCACTTGGAGGAGGACATCAACTGCGGGATTATAGCTCCAGATTTCGATGTGT 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
30274R-9.in silico     301 AGGCGCGCCACTTGGAGGAGGACATCAACTGCGGGATTATAGCTCCAGATTTCGATGTGT 360


30274R-9.IR_full       361 TTGTGGTGTTCCAAGAGTTCTTGGTGCCGTTGCTTAAGGACATGCATTGCCTGAGTATCG 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
30274R-9.in silico     361 TTGTGGTGTTCCAAGAGTTCTTGGTGCCGTTGCTTAAGGACATGCATTGCCTGAGTATCG 420


30274R-9.IR_full       421 ATGCTGATTTCAAACCGCAACCTCGACTGGCCTATTTTCCCATGGACAATGGCGAAAGGC 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
30274R-9.in silico     421 ATGCTGATTTCAAACCGCAACCTCGACTGGCCTATTTTCCCATGGACAATGGCGAAAGGC 480


30274R-9.IR_full       481 TCAACACAGCGGCTTTCGTCTTC 503
                           |||||||||||||||||||||||
30274R-9.in silico     481 TCAACACAGCGGCTTTCGTCTTC 503



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  4  NM_166563.1  CG30274-RA (CG30274), mRNA 
0.41   2  2  2  2  NM_080125.2  CG4399-RB (east), mRNA 
0   0  4  2  2  NM_079728.4  CG31289-RA (Dph5), mRNA 
0   0  2  5  4  NM_169801.1  CG31241-RA (CG31241), mRNA 
0   0  2  4  4  NM_134722.1  CG14339-RA (CG14339), mRNA 
0   0  2  4  2  NM_141431.1  CG1105-RA (CG1105), mRNA 
0   0  2  3  4  NM_079488.3  CG9054-RA (Ddx1), mRNA 
0   0  2  3  2  NM_167460.1  CG8944-RA, transcript variant A (CG8944), mRNA 
0   0  2  3  2  NM_132837.1  CG8944-RB, transcript variant B (CG8944), mRNA 
0   0  1  6  5  NM_164639.1  CG14029-RC, transcript variant C (vri), mRNA 
0   0  1  5  3  NM_057247.3  CG9075-RC, transcript variant C (eIF-4a), mRNA 
0   0  1  5  3  NM_164668.1  CG9075-RA, transcript variant A (eIF-4a), mRNA 
0   0  1  5  3  NM_164669.1  CG9075-RB, transcript variant B (eIF-4a), mRNA 
0   0  1  5  3  NM_164670.1  CG9075-RD, transcript variant D (eIF-4a), mRNA 
0   0  1  4  4  NM_141072.2  CG7177-RA (CG7177), mRNA 
0   0  1  3  9  NM_001043298.1  CG33553-RH, transcript variant H (Doa), mRNA 
0   0  1  3  9  NM_001014681.1  CG33553-RD, transcript variant D (Doa), mRNA 
0   0  1  3  8  NM_176104.1  CG33087-RC (CG33087), mRNA 
0   0  1  3  2  NM_167911.1  CG2086-RB, transcript variant B (drpr), mRNA 
0   0  1  3  2  NM_058102.2  CG2086-RA, transcript variant A (drpr), mRNA 
0   0  1  2  8  NM_137493.2  CG30116-RC, transcript variant C (CG30116), mRNA 
0   0  1  2  8  NM_137494.1  CG30116-RB, transcript variant B (CG30116), mRNA 
0   0  1  2  8  NM_166291.1  CG30116-RD, transcript variant D (CG30116), mRNA 
0   0  1  2  8  NM_166292.1  CG30116-RA, transcript variant A (CG30116), mRNA 
0   0  1  2  6  NM_142284.2  CG14881-RB, transcript variant B (CG14881), mRNA 
0   0  1  2  6  NM_169718.1  CG14881-RA, transcript variant A (CG14881), mRNA 
0   0  0  7  7  NM_139462.1  CG13800-RA (CG13800), mRNA 
0   0  0  6  3  NM_079180.2  CG10579-RD, transcript variant D (Eip63E), mRNA 
0   0  0  6  3  NM_168033.1  CG10579-RE, transcript variant E (Eip63E), mRNA 
0   0  0  6  3  NM_001043116.1  CG10579-RF, transcript variant F (Eip63E), mRNA 

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