NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 30273R-6

Symbol CG30273 Full Name CG30273

CG No CG30273 Old CG No CG30273

Synonyms CG13515, CG30273

Accession No (Link to NCBI) NM_166542.1

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees viable

Map Viewer

Reference
[Please submit your publication] Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgTACAATCAGCAGCCGGCGGGA

Primer Seq. 3'

aatctagaggtaccATCGCTGTTGTAGACGCCGAC

Predicted Fragment Size

IR fragment full Seq

0001 TACAATCAGC AGCCGGCGGG AGCGCCgggC TATCCCCACC AGATGCCCCC ACCGAGCTAT 
0061 GAGCAGGTGG TGCACGTGCA GGCGCCAGTT CGGGTGGTTC ACCACACGAC AGCACCTCCG 
0121 CCCGTGGTGA TCATACAACA TCAAGCTGTA TTGCCGGTGG GTCCGGATCC CACCTTCATC 
0181 ACCTGCCCGC ATTGCCATGT CCAGAAACTG ACCCGCGTGG AGTACTCGCC CAGCGTGNAA 
0241 AACTCACTGC ATGGCCGCCA TTCTATGCAT CGTTGGNCTC TGGTGTTGTG CCTGCCTGCC 
0301 TTACTGCGCC ACCAGCTGCA TGAACGCCAA TCACTTTTGC GGCAATTGCA ATAAGTTCGT 
0361 CGGCGTCTAC AACAGCGAT

in silico PCR Fragment

0001 TACAATCAGC AGCCGGCGGG AGCGCCGGGC TATCCCCACC AGATGCCCCC ACCGAGCTAT 
0061 GAGCAGGTGG TGCACGTGCA GGCGCCAGTT CGGGTGGTTC ACCACACGAC AGCACCTCCG 
0121 CCCGTGGTGA TCATACAACA TCAAGCTGTA TTGCCGGTGG GTCCGGATCC CACCTTCATC 
0181 ACCTGCCCGC ATTGCCATGT CCAGAAACTG ACCCGCGTGG AGTACTCGCC CAGTGTG-AA 
0241 AACTCACTGC ATGGCCGCCA TTCTATGCAT CGTTGGCCTC TGGTGTTGTG CCTGCCTGCC 
0301 TTACTGCGCC ACCAGCTGCA TGAACGCCAA TCACTTTTGC GGCAATTGCA ATAAGTTCGT 
0361 CGGCGTCTAC AACAGCGAT

Assemble Data


30273R-6.IR_full       1   TACAATCAGCAGCCGGCGGGAGCGCCGGGCTATCCCCACCAGATGCCCCCACCGAGCTAT 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
30273R-6.in silico     1   TACAATCAGCAGCCGGCGGGAGCGCCGGGCTATCCCCACCAGATGCCCCCACCGAGCTAT 60


30273R-6.IR_full       61  GAGCAGGTGGTGCACGTGCAGGCGCCAGTTCGGGTGGTTCACCACACGACAGCACCTCCG 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
30273R-6.in silico     61  GAGCAGGTGGTGCACGTGCAGGCGCCAGTTCGGGTGGTTCACCACACGACAGCACCTCCG 120


30273R-6.IR_full       121 CCCGTGGTGATCATACAACATCAAGCTGTATTGCCGGTGGGTCCGGATCCCACCTTCATC 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
30273R-6.in silico     121 CCCGTGGTGATCATACAACATCAAGCTGTATTGCCGGTGGGTCCGGATCCCACCTTCATC 180


30273R-6.IR_full       181 ACCTGCCCGCATTGCCATGTCCAGAAACTGACCCGCGTGGAGTACTCGCCCAGCGTGNAA 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||| ||| ||
30273R-6.in silico     181 ACCTGCCCGCATTGCCATGTCCAGAAACTGACCCGCGTGGAGTACTCGCCCAGTGTG-AA 240


30273R-6.IR_full       241 AACTCACTGCATGGCCGCCATTCTATGCATCGTTGGNCTCTGGTGTTGTGCCTGCCTGCC 300
                           |||||||||||||||||||||||||||||||||||| |||||||||||||||||||||||
30273R-6.in silico     241 AACTCACTGCATGGCCGCCATTCTATGCATCGTTGGCCTCTGGTGTTGTGCCTGCCTGCC 300


30273R-6.IR_full       301 TTACTGCGCCACCAGCTGCATGAACGCCAATCACTTTTGCGGCAATTGCAATAAGTTCGT 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
30273R-6.in silico     301 TTACTGCGCCACCAGCTGCATGAACGCCAATCACTTTTGCGGCAATTGCAATAAGTTCGT 360


30273R-6.IR_full       361 CGGCGTCTACAACAGCGAT 379
                           |||||||||||||||||||
30273R-6.in silico     361 CGGCGTCTACAACAGCGAT 379

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	360	0	0	0	NM_166542.1	CG30273-RA (CG30273), mRNA
0.83	3	2	3	2	NM_058159.4	CG4700-RA, transcript variant A (Sema-2a), mRNA
0	0	4	5	2	NM_080048.2	CG10109-RA (L), mRNA
0	0	2	2	1	NM_167630.1	CG6891-RB, transcript variant B (CG6891), mRNA
0	0	1	6	4	NM_137860.2	CG30269-RA (CG30269), mRNA
0	0	1	6	2	NM_138229.1	CG13908-RA (CG13908), mRNA
0	0	1	5	35	NM_175960.3	CG33196-RB (dp), mRNA
0	0	1	4	6	NM_143665.2	CG2041-RA (lgs), mRNA
0	0	1	4	2	NM_133075.2	CG6461-RA (CG6461), mRNA
0	0	1	3	3	NM_139766.1	CG6619-RA (CG6619), mRNA
0	0	1	3	3	NM_134531.2	CG9577-RA (CG9577), mRNA
0	0	1	3	2	NM_001043028.1	CG17800-RR, transcript variant R (Dscam), mRNA
0	0	1	3	2	NM_001043030.1	CG17800-RX, transcript variant X (Dscam), mRNA
0	0	1	2	3	NM_058131.3	CG6712-RA (CG6712), mRNA
0	0	1	1	3	NM_168366.2	CG32046-RB, transcript variant B (CG32046), mRNA
0	0	1	1	3	NM_168367.1	CG32046-RA, transcript variant A (CG32046), mRNA
0	0	0	6	14	NM_001032051.1	CG33715-RD, transcript variant D (Msp-300), mRNA
0	0	0	6	2	NM_058026.3	CG5709-RA (ari-2), mRNA
0	0	0	5	4	NM_142701.2	CG5874-RA (CG5874), mRNA
0	0	0	4	5	NM_135077.2	CG14023-RA (CG14023), mRNA
0	0	0	4	4	NM_164966.2	CG6464-RA (salm), mRNA
0	0	0	4	3	NM_167193.1	CG32702-RA (CG32702), mRNA
0	0	0	4	3	NM_167330.2	CG17762-RD, transcript variant D (tomosyn), mRNA
0	0	0	4	3	NM_164797.1	CG8475-RB, transcript variant B (CG8475), mRNA
0	0	0	4	3	NM_135345.2	CG8475-RA, transcript variant A (CG8475), mRNA
0	0	0	4	2	NM_137530.1	CG15080-RA (CG15080), mRNA
0	0	0	3	9	NM_001032403.1	CG7649-RB, transcript variant B (Neu3), mRNA
0	0	0	3	8	NM_001032053.1	CG33715-RB, transcript variant B (Msp-300), mRNA
0	0	0	3	7	NM_167654.1	CG3400-RF, transcript variant F (Pfrx), mRNA
0	0	0	3	7	NM_167651.1	CG3400-RA, transcript variant A (Pfrx), mRNA

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