|
Notice:
|
|
Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
|
|
| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
discarded
|
| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
30185R-1 |
| Symbol |
CG30185 |
Full Name |
CG30185 |
| CG No |
CG30185 |
Old CG No |
CG30185 |
| Synonyms |
CG30185, CG5365, anon-EST:Posey25 |
| Accession No (Link to NCBI) |
NM_145193.1 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgTGTGACGTTGCAACAGTGCAG |
| Primer Seq. 3' |
aatctagaggtaccATGGTGGTGAAGTTCAGCAGT |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 TGTGACGTTG CNACAGTGCN GAAGATAGCA NACTGCCTGG GCGTTAATCC CGGCAAGNTG
0061 CAGCTGAACG AGGAGCNGGT CGTAACGCGC ACGAGTGGGC AAAAGAAGAG CGTGGCCGGA
0121 TTCGCCACGA TCCTGGAGAG CCTGGCCAGC GAGTCCAAGT CGGAGAcCGC CCAGAACAGC
0181 AGGGCGTCGC GGGAGGTGGA AGCCCAGGTG TACCAGTGGA TCGAGTTCTC TGTGCTCTAC
0241 GTGGCGCCCG GCTCCAAGGA CAAGTACGTG TCCAAGCAGC TTCTGGCGGA CTTCAACAAA
0301 CTGTTTGCCA GCAAATCCTA CCTGGTGGGC CACTTCATCA CTCTAGCCGA CCTGGCCGTC
0361 TACTATGCCA TCTACGATCT TGTGAAATCC CTTTCGCCGG TGGACAAGGA GGTATATTTG
0421 AATCTCTcCC GCTGGTTCGA TCACCTCCAG AATCGCGCGG ATGTGCACCA GGGGCGAGCC
0481 ACTGCTGAAC TTCACCACCA T |
| in silico PCR Fragment |
0001 TGTGACGTTG CAACAGTGCA GAAGATAGCA AACTGCCTGG GCGTTAATCC CGGCAAGGTG
0061 CAGCTGAACG AGGAGCAGGT CGTAACGCGC ACGAGTGGGC AAAAGAAGAG CGTGGCCGGA
0121 TTCGCCTCGA TCCTGGAGAG CCTGGCCAGC GAGTCCAAGT CGGAGACCGC CCAGAACAGC
0181 AGGGCGTCGC GGGAGGTGGA AGCCCAGGTG TACCAGTGGA TCGAGTTCTC CGTGCTCTAC
0241 GTGGCGCCCG GCTCCAAGGA CAAGTACGTG TCCAAGCAGC TTCTGGCGGA CTTCAACAAA
0301 CTGTTTGCCA GCAAATCCTA CCTGGTGGGC CACTTCATCA CTCTAGCCGA CCTGGCCGTC
0361 TACTATGCCA TCTACGATCT TGTGAAATCC CTTTCGCCGG TGGACAAGGA GGTATATTTG
0421 AATCTCTCCC GCTGGTTCGA TCACCTCCAG AATCGCGCGG ATGTGCACCA -GGGCGAGCC
0481 ACTGCTGAAC TTCACCACCA T |
| Assemble Data |
30185R-1.IR_full 1 TGTGACGTTGCNACAGTGCNGAAGATAGCANACTGCCTGGGCGTTAATCCCGGCAAGNTG 60
||||||||||| ||||||| |||||||||| |||||||||||||||||||||||||| ||
30185R-1.in silico 1 TGTGACGTTGCAACAGTGCAGAAGATAGCAAACTGCCTGGGCGTTAATCCCGGCAAGGTG 60
30185R-1.IR_full 61 CAGCTGAACGAGGAGCNGGTCGTAACGCGCACGAGTGGGCAAAAGAAGAGCGTGGCCGGA 120
|||||||||||||||| |||||||||||||||||||||||||||||||||||||||||||
30185R-1.in silico 61 CAGCTGAACGAGGAGCAGGTCGTAACGCGCACGAGTGGGCAAAAGAAGAGCGTGGCCGGA 120
30185R-1.IR_full 121 TTCGCCACGATCCTGGAGAGCCTGGCCAGCGAGTCCAAGTCGGAGACCGCCCAGAACAGC 180
|||||| |||||||||||||||||||||||||||||||||||||||||||||||||||||
30185R-1.in silico 121 TTCGCCTCGATCCTGGAGAGCCTGGCCAGCGAGTCCAAGTCGGAGACCGCCCAGAACAGC 180
30185R-1.IR_full 181 AGGGCGTCGCGGGAGGTGGAAGCCCAGGTGTACCAGTGGATCGAGTTCTCTGTGCTCTAC 240
|||||||||||||||||||||||||||||||||||||||||||||||||| |||||||||
30185R-1.in silico 181 AGGGCGTCGCGGGAGGTGGAAGCCCAGGTGTACCAGTGGATCGAGTTCTCCGTGCTCTAC 240
30185R-1.IR_full 241 GTGGCGCCCGGCTCCAAGGACAAGTACGTGTCCAAGCAGCTTCTGGCGGACTTCAACAAA 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
30185R-1.in silico 241 GTGGCGCCCGGCTCCAAGGACAAGTACGTGTCCAAGCAGCTTCTGGCGGACTTCAACAAA 300
30185R-1.IR_full 301 CTGTTTGCCAGCAAATCCTACCTGGTGGGCCACTTCATCACTCTAGCCGACCTGGCCGTC 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
30185R-1.in silico 301 CTGTTTGCCAGCAAATCCTACCTGGTGGGCCACTTCATCACTCTAGCCGACCTGGCCGTC 360
30185R-1.IR_full 361 TACTATGCCATCTACGATCTTGTGAAATCCCTTTCGCCGGTGGACAAGGAGGTATATTTG 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
30185R-1.in silico 361 TACTATGCCATCTACGATCTTGTGAAATCCCTTTCGCCGGTGGACAAGGAGGTATATTTG 420
30185R-1.IR_full 421 AATCTCTCCCGCTGGTTCGATCACCTCCAGAATCGCGCGGATGTGCACCAGGGGCGAGCC 480
|||||||||||||||||||||||||||||||||||||||||||||||||| |||||||||
30185R-1.in silico 421 AATCTCTCCCGCTGGTTCGATCACCTCCAGAATCGCGCGGATGTGCACCA-GGGCGAGCC 480
30185R-1.IR_full 481 ACTGCTGAACTTCACCACCAT 501
|||||||||||||||||||||
30185R-1.in silico 481 ACTGCTGAACTTCACCACCAT 501
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_145193.1 |
CG30185-RA (CG30185), mRNA |
| 0.2 |
1 |
2 |
2 |
2 |
NM_141251.1 |
CG2016-RB (CG2016), mRNA |
| 0 |
0 |
3 |
5 |
3 |
NM_140567.2 |
CG6017-RA (CG6017), mRNA |
| 0 |
0 |
2 |
4 |
4 |
NM_079381.2 |
CG4899-RA, transcript variant A (Pdh), mRNA |
| 0 |
0 |
2 |
4 |
4 |
NM_168659.1 |
CG4899-RB, transcript variant B (Pdh), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_132120.1 |
CG14438-RA, transcript variant A (CG14438), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_167090.1 |
CG14438-RB, transcript variant B (CG14438), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_001032405.1 |
CG33956-RE, transcript variant E (kay), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_001032406.1 |
CG33956-RB, transcript variant B (kay), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_001032407.1 |
CG33956-RA, transcript variant A (kay), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_001032408.1 |
CG33956-RD, transcript variant D (kay), mRNA |
| 0 |
0 |
2 |
3 |
1 |
NM_176567.1 |
CG33102-RA (Hex-t1), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_133116.1 |
CG14190-RA (CG14190), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_058091.3 |
CG3152-RA (Trap1), mRNA |
| 0 |
0 |
1 |
7 |
3 |
NM_130626.2 |
CG3835-RA, transcript variant A (CG3835), mRNA |
| 0 |
0 |
1 |
7 |
3 |
NM_166930.1 |
CG3835-RC, transcript variant C (CG3835), mRNA |
| 0 |
0 |
1 |
7 |
3 |
NM_166929.1 |
CG3835-RB, transcript variant B (CG3835), mRNA |
| 0 |
0 |
1 |
4 |
6 |
NM_134916.3 |
CG9663-RA (CG9663), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_001014695.1 |
CG11153-RB, transcript variant B (Sox102F), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_166792.1 |
CG11153-RA, transcript variant A (Sox102F), mRNA |
| 0 |
0 |
1 |
3 |
8 |
NM_164935.2 |
CG31721-RA (Trim9), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_167675.1 |
CG14213-RA, transcript variant A (CG14213), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_134491.2 |
CG14213-RB, transcript variant B (CG14213), mRNA |
| 0 |
0 |
1 |
2 |
8 |
NM_001043114.1 |
CG33484-RD, transcript variant D (zormin), mRNA |
| 0 |
0 |
1 |
2 |
8 |
NM_001043112.1 |
CG33484-RB, transcript variant B (zormin), mRNA |
| 0 |
0 |
1 |
2 |
8 |
NM_206240.1 |
CG33484-RA, transcript variant A (zormin), mRNA |
| 0 |
0 |
1 |
2 |
8 |
NM_001043113.1 |
CG33484-RC, transcript variant C (zormin), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_143391.1 |
CG11828-RA (CG11828), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_143145.1 |
CG5111-RA (CG5111), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_167398.1 |
CG32599-RA (CG32599), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
JNK signal
| N |
 |
| |
|
| DB |
 |
| +- |
|
| DC |
 |
| + |
|
| DM |
 |
| +- |
|
| VM |
 |
| |
|
| VB |
 |
| +- |
|
|
Caspase-3 signal
| N |
|
| ++ |
|
| DB |
|
| + |
|
| DC |
|
| ++ |
|
| DM |
|
| ++ |
|
| VM |
|
| ++ |
|
| VB |
|
| + |
|
|
|
|
|
|
