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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 30165R-2 
 Symbol CG30165  Full Name CG30165 
 CG No CG30165  Old CG No CG30165 
 Synonyms CG4556, CG30165 
 Accession No (Link to NCBI) NM_166670.1 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees semi-lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgGGGACTACAGGGATCGCTTGA 
 Primer Seq. 3' 
aatctagaggtaccCGGGGTGGATTCTTCGTCTGC 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 GGGACTACAG GGATCGCTTG AAGGCCTACT TGACCACCAG TAAGGCAACC ATGGAGTCCC 
0061 AGCTGGGCGA CACTACGACC AAGTGCCGAC CCTTCTACGA CACCTTCGAC GCCAGCCGCA 
0121 CCTTCCTCTG CCGCAACATG GTGGATTTCA TCAACGGCCT CACCTTCTTC ATCTTCCTTA 
0181 CACTGCTTCT CTGGGCTATT GGCACTCCGG TGGGCCTCAA CCTGATCACG GTTCAAACGC 
0241 GGCTGAATGT GATGCAGGCG GCTCAGGCGC GGAGCAAGGG CAGGCACAGG AGGAGCGACC 
0301 GGAGTCCGGA GCATGAGCAG AGGAGCGGAA GTCGCGGCCG GAAGAAGCAA CGGACGAGCA 
0361 GTTCAGCGAC AAGCGGGCGG AGCGGAAAGA GGGACCAGAG CCGGGAGAGG AGCACCAGCA 
0421 GCACGGCGAG ACCGAAACCA CCTTTAAGAC GGACTGCCAC TGAAGAAACA CTTGATCTGG 
0481 CAGACGAAGA ATCCACCCCG  
 in silico PCR Fragment 
0001 GGGACTACAG GGATCGCTTG AAGGCCTACT TGACCACCAG TAAGGCAACC ATGGAGTCCC 
0061 AGCTGGGCGA CACTACGACC AAGTGCCGAC CCTTCTACGA CACCTTCGAC GCCAGCCGCA 
0121 CCTTCCTCTG CCGCAACATG GTGGATTTCA TCAACGGCCT CACCTTCTTC ATCTTCCTTA 
0181 CACTGCTTCT CTGGGCTATT GGCACTCCGG TGGGCCTCAA CCTGATCACG GTTCAAACGC 
0241 GGCTGAATGT GATGCAGGCG GCTCAGGCGC GGAGCAAGGG CAGGCACAGG AGGAGCGACC 
0301 GGAGTCCGGA GCATGAGCAG AGGAGCGGAA GTCGCGGCCG GAAGAAGCAA CGGACGAGCA 
0361 GTTCAGCGAC AAGCGGGCGG AGCGGAAAGA GGGACCAGAG CCGGGAGAGG AGCACCAGCA 
0421 GCACGGCGAG ACCGAAACCA CCTTTAAGAC GGACTGCCAC TGAAGAAACA CTTGATCTGG 
0481 CAGACGAAGA ATCCACCCCG  
 Assemble Data 

30165R-2.IR_full       1   GGGACTACAGGGATCGCTTGAAGGCCTACTTGACCACCAGTAAGGCAACCATGGAGTCCC 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
30165R-2.in silico     1   GGGACTACAGGGATCGCTTGAAGGCCTACTTGACCACCAGTAAGGCAACCATGGAGTCCC 60


30165R-2.IR_full       61  AGCTGGGCGACACTACGACCAAGTGCCGACCCTTCTACGACACCTTCGACGCCAGCCGCA 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
30165R-2.in silico     61  AGCTGGGCGACACTACGACCAAGTGCCGACCCTTCTACGACACCTTCGACGCCAGCCGCA 120


30165R-2.IR_full       121 CCTTCCTCTGCCGCAACATGGTGGATTTCATCAACGGCCTCACCTTCTTCATCTTCCTTA 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
30165R-2.in silico     121 CCTTCCTCTGCCGCAACATGGTGGATTTCATCAACGGCCTCACCTTCTTCATCTTCCTTA 180


30165R-2.IR_full       181 CACTGCTTCTCTGGGCTATTGGCACTCCGGTGGGCCTCAACCTGATCACGGTTCAAACGC 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
30165R-2.in silico     181 CACTGCTTCTCTGGGCTATTGGCACTCCGGTGGGCCTCAACCTGATCACGGTTCAAACGC 240


30165R-2.IR_full       241 GGCTGAATGTGATGCAGGCGGCTCAGGCGCGGAGCAAGGGCAGGCACAGGAGGAGCGACC 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
30165R-2.in silico     241 GGCTGAATGTGATGCAGGCGGCTCAGGCGCGGAGCAAGGGCAGGCACAGGAGGAGCGACC 300


30165R-2.IR_full       301 GGAGTCCGGAGCATGAGCAGAGGAGCGGAAGTCGCGGCCGGAAGAAGCAACGGACGAGCA 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
30165R-2.in silico     301 GGAGTCCGGAGCATGAGCAGAGGAGCGGAAGTCGCGGCCGGAAGAAGCAACGGACGAGCA 360


30165R-2.IR_full       361 GTTCAGCGACAAGCGGGCGGAGCGGAAAGAGGGACCAGAGCCGGGAGAGGAGCACCAGCA 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
30165R-2.in silico     361 GTTCAGCGACAAGCGGGCGGAGCGGAAAGAGGGACCAGAGCCGGGAGAGGAGCACCAGCA 420


30165R-2.IR_full       421 GCACGGCGAGACCGAAACCACCTTTAAGACGGACTGCCACTGAAGAAACACTTGATCTGG 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
30165R-2.in silico     421 GCACGGCGAGACCGAAACCACCTTTAAGACGGACTGCCACTGAAGAAACACTTGATCTGG 480


30165R-2.IR_full       481 CAGACGAAGAATCCACCCCG 500
                           ||||||||||||||||||||
30165R-2.in silico     481 CAGACGAAGAATCCACCCCG 500



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_166670.1  CG30165-RA (CG30165), mRNA 
0.2   1  2  3  13  NM_176447.1  CG33208-RF, transcript variant F (MICAL), mRNA 
0.2   1  2  3  13  NM_176445.1  CG33208-RC, transcript variant C (MICAL), mRNA 
0.2   1  2  3  13  NM_176449.1  CG33208-RH, transcript variant H (MICAL), mRNA 
0.2   1  2  3  13  NM_176448.1  CG33208-RG, transcript variant G (MICAL), mRNA 
0.2   1  2  3  13  NM_176446.1  CG33208-RD, transcript variant D (MICAL), mRNA 
0.2   1  2  3  11  NM_176444.1  CG33208-RB, transcript variant B (MICAL), mRNA 
0   0  4  2  2  NM_140407.1  CG8757-RA (CG8757), mRNA 
0   0  3  5  3  NM_166087.1  CG8153-RC, transcript variant C (mus210), mRNA 
0   0  3  5  3  NM_057513.3  CG8153-RA, transcript variant A (mus210), mRNA 
0   0  3  4  8  NM_143168.1  CG17197-RA (CG17197), mRNA 
0   0  2  4  3  NM_080306.2  CG3443-RB (pcx), mRNA 
0   0  2  3  3  NM_140270.2  CG7252-RA (CG7252), mRNA 
0   0  1  7  6  NM_001042895.1  CG31731-RB, transcript variant B (CG31731), mRNA 
0   0  1  7  6  NM_165058.3  CG31731-RA, transcript variant A (CG31731), mRNA 
0   0  1  5  3  NM_079765.2  CG13633-RA (Ast), mRNA 
0   0  1  4  4  NM_079393.2  CG4083-RA (Mo25), mRNA 
0   0  1  4  3  NM_137629.1  CG13871-RA (CG13871), mRNA 
0   0  1  4  2  NM_141108.1  CG7414-RA (CG7414), mRNA 
0   0  1  3  4  NM_135778.1  CG16815-RA (CG16815), mRNA 
0   0  1  3  3  NM_079055.2  CG4943-RA (lack), mRNA 
0   0  1  2  8  NM_169700.2  CG31045-RA, transcript variant A (Mhcl), mRNA 
0   0  1  2  8  NM_001043250.1  CG31045-RG, transcript variant G (Mhcl), mRNA 
0   0  1  2  8  NM_169701.2  CG31045-RB, transcript variant B (Mhcl), mRNA 
0   0  1  2  4  NM_143617.1  CG1638-RA (CG1638), mRNA 
0   0  1  2  2  NM_135977.1  CG15133-RA (CG15133), mRNA 
0   0  0  5  10  NM_132295.1  CG6999-RA (CG6999), mRNA 
0   0  0  5  7  NM_140858.2  CG18294-RA (CG18294), mRNA 
0   0  0  5  7  NM_140857.2  CG12519-RA (CG12519), mRNA 
0   0  0  5  7  NM_140856.1  CG14096-RA (CG14096), mRNA 

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