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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 30005R-2 
 Symbol CG30005  Full Name CG30005 
 CG No CG30005  Old CG No CG30005 
 Synonyms CG12930A, CG12930, CG30005 
 Accession No (Link to NCBI) NM_165690.2 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgCCCGTGGTCTATGGATTGGTT 
 Primer Seq. 3' 
aatctagaggtaccCACACTCAGCTGGTTGATTTC 
 Predicted Fragment Size 
497 
 IR fragment full Seq 
0001 cCCNTGGNTC TATGGATTGG TTTAAAATTC AGCAACTCAC CCGTTGAGTA TTGCCCGATT 
0061 GCCCTGCGAA AATTTGAGCA ATTGACCGAC GAGTACAAGA AGATCAATCG ATTCCAGAAG 
0121 GTGCCCGCCA TTGTGGATGG GGACTTCCAT TTGTCTGAGA CCATAGCGAT TATAAGATAT 
0181 CTCGCAGACA AAGGTCAGTT CGATGAGAAG CTCTACCCAA AGACGCTGGA GAACCGTGCC 
0241 CGAGTGGATG AGTTCCTTGA GTGGCAACAC CTGAACATTC GTCTTGCATG TTCCATGTAC 
0301 TTTCGAGATG CCTGGCTGTT TCCCATGAAC GGGATAGCGC CCAAGCCCAA GCCAGAGCAG 
0361 ATTCAAGCAC TGATTGAGGG AGTGGAAAAC AATCTGGGTT TACTGGAGCG CCTGTGGCTG 
0421 GAAAATGATT TTTTGGTTGG GAAAAACCTG ACAATGGCTG ATATCCTTGG CTCTTCCGAA 
0481 ATCAACCAGC TGAGTGTA 
 in silico PCR Fragment 
0001 CCCGTGG-TC TATGGATTGG TTTAAAATTC AGCAACTCAC CCGTTGAGTA TTGCCCGATT 
0061 GCCCTGCGAA AATTTGAGCA ATTGACCGAC GAGTACAAGA AGATCAATCG ATTCCAGAAG 
0121 GTGCCCGCCA TTGTGGGTGG GGACTTCCAT TTGTCTGAGA CCATAGCGAT TATAAGATAT 
0181 CTCGCAGACA AAGGTCAGTT CGATGAGAAG CTCTACCCAA AGACGCTGGA GAACCGTGCC 
0241 CGAGTGGATG AGTTCCTTGA GTGGCAACAC CTGAACATTC GTCTTGCATG TTCCATGTAC 
0301 TTTCGAGATG CCTGGCTGTT TCCCATGAAC GGGATAGCGC CCAAGCCCAA GCCAGAGCAG 
0361 ATTCAAGCAC TGATTGAGGG AGTGGAAAAC AATCTGGGTT TACTGGAGCG CCTGTGGCTG 
0421 GAAAATGATT TTTTGGTTGG GAAAAACCTG ACAATGGCTG ATATCCTTGG CTCTTCCGAA 
0481 ATCAACCAGC TGAGTGTA 
 Assemble Data 

30005R-2.IR_full       1   CCCNTGGNTCTATGGATTGGTTTAAAATTCAGCAACTCACCCGTTGAGTATTGCCCGATT 60
                           ||| ||| ||||||||||||||||||||||||||||||||||||||||||||||||||||
30005R-2.in silico     1   CCCGTGG-TCTATGGATTGGTTTAAAATTCAGCAACTCACCCGTTGAGTATTGCCCGATT 60


30005R-2.IR_full       61  GCCCTGCGAAAATTTGAGCAATTGACCGACGAGTACAAGAAGATCAATCGATTCCAGAAG 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
30005R-2.in silico     61  GCCCTGCGAAAATTTGAGCAATTGACCGACGAGTACAAGAAGATCAATCGATTCCAGAAG 120


30005R-2.IR_full       121 GTGCCCGCCATTGTGGATGGGGACTTCCATTTGTCTGAGACCATAGCGATTATAAGATAT 180
                           |||||||||||||||| |||||||||||||||||||||||||||||||||||||||||||
30005R-2.in silico     121 GTGCCCGCCATTGTGGGTGGGGACTTCCATTTGTCTGAGACCATAGCGATTATAAGATAT 180


30005R-2.IR_full       181 CTCGCAGACAAAGGTCAGTTCGATGAGAAGCTCTACCCAAAGACGCTGGAGAACCGTGCC 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
30005R-2.in silico     181 CTCGCAGACAAAGGTCAGTTCGATGAGAAGCTCTACCCAAAGACGCTGGAGAACCGTGCC 240


30005R-2.IR_full       241 CGAGTGGATGAGTTCCTTGAGTGGCAACACCTGAACATTCGTCTTGCATGTTCCATGTAC 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
30005R-2.in silico     241 CGAGTGGATGAGTTCCTTGAGTGGCAACACCTGAACATTCGTCTTGCATGTTCCATGTAC 300


30005R-2.IR_full       301 TTTCGAGATGCCTGGCTGTTTCCCATGAACGGGATAGCGCCCAAGCCCAAGCCAGAGCAG 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
30005R-2.in silico     301 TTTCGAGATGCCTGGCTGTTTCCCATGAACGGGATAGCGCCCAAGCCCAAGCCAGAGCAG 360


30005R-2.IR_full       361 ATTCAAGCACTGATTGAGGGAGTGGAAAACAATCTGGGTTTACTGGAGCGCCTGTGGCTG 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
30005R-2.in silico     361 ATTCAAGCACTGATTGAGGGAGTGGAAAACAATCTGGGTTTACTGGAGCGCCTGTGGCTG 420


30005R-2.IR_full       421 GAAAATGATTTTTTGGTTGGGAAAAACCTGACAATGGCTGATATCCTTGGCTCTTCCGAA 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
30005R-2.in silico     421 GAAAATGATTTTTTGGTTGGGAAAAACCTGACAATGGCTGATATCCTTGGCTCTTCCGAA 480


30005R-2.IR_full       481 ATCAACCAGCTGAGTGTA 498
                           ||||||||||||||||||
30005R-2.in silico     481 ATCAACCAGCTGAGTGTA 498



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   479  0  0  2  NM_165690.2  CG30005-RA (CG30005), mRNA 
0   0  5  29  58  NM_136665.3  CG30000-RA (CG30000), mRNA 
0   0  2  4  3  NM_080715.3  CG12223-RC, transcript variant C (Dsp1), mRNA 
0   0  2  4  3  NM_167502.2  CG12223-RD, transcript variant D (Dsp1), mRNA 
0   0  2  4  3  NM_164744.1  CG4675-RB, transcript variant B (Ndae1), mRNA 
0   0  2  4  3  NM_078777.2  CG4675-RA, transcript variant A (Ndae1), mRNA 
0   0  2  2  3  NM_001038730.1  CG3711-RB, transcript variant B (CG3711), mRNA 
0   0  2  2  3  NM_130513.2  CG3711-RA, transcript variant A (CG3711), mRNA 
0   0  2  2  2  NM_140740.2  CG6259-RA (CG6259), mRNA 
0   0  1  7  2  NM_164554.2  CG2822-RB, transcript variant B (Shaw), mRNA 
0   0  1  3  4  NM_138258.2  CG2211-RA (CG2211), mRNA 
0   0  1  3  2  NM_135791.1  CG15639-RA (CG15639), mRNA 
0   0  1  2  3  NM_168171.1  CG32397-RA (CG32397), mRNA 
0   0  1  2  2  NM_140938.1  CG13813-RA (CG13813), mRNA 
0   0  0  5  5  NM_166863.1  CG5273-RC, transcript variant C (CG5273), mRNA 
0   0  0  5  5  NM_130501.3  CG5273-RA, transcript variant A (CG5273), mRNA 
0   0  0  5  4  NM_166862.1  CG5273-RB, transcript variant B (CG5273), mRNA 
0   0  0  5  3  NM_170653.1  CG6643-RB, transcript variant B (CG6643), mRNA 
0   0  0  4  9  NM_143104.1  CG11856-RA (Nup358), mRNA 
0   0  0  4  8  NM_079564.3  CG8874-RA, transcript variant A (Fps85D), mRNA 
0   0  0  4  8  NM_169274.1  CG8874-RB, transcript variant B (Fps85D), mRNA 
0   0  0  4  8  NM_169275.1  CG8874-RC, transcript variant C (Fps85D), mRNA 
0   0  0  4  5  NM_001038738.1  CG7803-RB, transcript variant B (z), mRNA 
0   0  0  4  5  NM_080312.2  CG7803-RA, transcript variant A (z), mRNA 
0   0  0  4  5  NM_079176.1  CG12008-RA, transcript variant A (kst), mRNA 
0   0  0  4  5  NM_206265.1  CG12008-RC, transcript variant C (kst), mRNA 
0   0  0  4  5  NM_206266.1  CG12008-RB, transcript variant B (kst), mRNA 
0   0  0  4  3  NM_079140.2  CG2679-RA, transcript variant A (gol), mRNA 
0   0  0  4  3  NM_164484.1  CG31684-RA (CG31684), mRNA 
0   0  0  4  3  NM_079054.3  CG6493-RA (Dcr-2), mRNA 

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