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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
2960R-1 |
| Symbol |
RpL40 |
Full Name |
Ribosomal protein L40 |
| CG No |
CG2960 |
Old CG No |
CG2960 |
| Synonyms |
Ub52, CEP52, DUb52, Ubi-f52, Ubi-f, Dub52, CG2960, BcDNA:RE10554, RpL40, Ubiq |
| Accession No (Link to NCBI) |
NM_057428.3 |
| Inserted Chr. |
lll |
| Insertional Mutation |
lethal |
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
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| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgTCGTGAAAACCCTCACCGGCA |
| Primer Seq. 3' |
aatctagaggtaccCTTCAACTTCTTCTTGGGGCG |
| Predicted Fragment Size |
374 |
| IR fragment full Seq |
0001 TCGTGAAAAC CCTCACCGGC AAGACCATCA CCTTGGAGGT GGAGCCTTCT GACACCATCG
0061 AGAATGTCAA GGCTAAGATC CAGGATAAGG AGGGCATTCC CCCAGATCAG CAGCGTCTGA
0121 TCTTCGCCGG CAAGCAGCTG GAGGATGGCC GCACTCTGTC CGACTACAAC ATTCAGAAGG
0181 AGTCCACCCT GCACTTGGTN NTCCGCCNTG CGTGGTGGTA TGCATTGAGC CCTCGCTCAG
0241 GATTCTGGCC CAGAAGTACA ACTGCGACAA GATGATCTGC CGCAAGTgCT AGCGCCCGTC
0301 TGCATCCTCG TGCCACCAAC TGCCGCAAGA AGAAGTGCGG ACACACCAAC AACCTGCGCC
0361 CCAAGAAGAA GTTGAAG |
| in silico PCR Fragment |
0001 TCGTGAAAAC CCTCACCGGC AAGACCATCA CCTTGGAGGT GGAGCCTTCT GACACCATCG
0061 AGAATGTCAA GGCTAAGATC CAGGATAAGG AGGGCATTCC CCCAGATCAG CAGCGTCTGA
0121 TCTTCGCCGG CAAGCAGCTG GAGGATGGCC GCACTCTGTC CGACTACAAC ATTCAGAAGG
0181 AGTCCACCCT GCACTTGGTG CTCCGCC-TG CGTGGTGGTA T-CATTGAGC CCTCGCTCAG
0241 GATTCTGGCC CAGAAGTACA ACTGCGACAA GATGATCTGC CGCAAGTGCT A-CGCCCGTC
0301 TGCATCCTCG TGCCACCAAC TGCCGCAAGA AGAAGTGCGG ACACACCAAC AACCTGCGCC
0361 CCAAGAAGAA GTTGAAG |
| Assemble Data |
2960R-1.IR_full 1 TCGTGAAAACCCTCACCGGCAAGACCATCACCTTGGAGGTGGAGCCTTCTGACACCATCG 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2960R-1.in silico 1 TCGTGAAAACCCTCACCGGCAAGACCATCACCTTGGAGGTGGAGCCTTCTGACACCATCG 60
2960R-1.IR_full 61 AGAATGTCAAGGCTAAGATCCAGGATAAGGAGGGCATTCCCCCAGATCAGCAGCGTCTGA 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2960R-1.in silico 61 AGAATGTCAAGGCTAAGATCCAGGATAAGGAGGGCATTCCCCCAGATCAGCAGCGTCTGA 120
2960R-1.IR_full 121 TCTTCGCCGGCAAGCAGCTGGAGGATGGCCGCACTCTGTCCGACTACAACATTCAGAAGG 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2960R-1.in silico 121 TCTTCGCCGGCAAGCAGCTGGAGGATGGCCGCACTCTGTCCGACTACAACATTCAGAAGG 180
2960R-1.IR_full 181 AGTCCACCCTGCACTTGGTNNTCCGCCNTGCGTGGTGGTATGCATTGAGCCCTCGCTCAG 240
||||||||||||||||||| |||||| ||||||||||||| ||||||||||||||||||
2960R-1.in silico 181 AGTCCACCCTGCACTTGGTGCTCCGCC-TGCGTGGTGGTAT-CATTGAGCCCTCGCTCAG 240
2960R-1.IR_full 241 GATTCTGGCCCAGAAGTACAACTGCGACAAGATGATCTGCCGCAAGTGCTAGCGCCCGTC 300
||||||||||||||||||||||||||||||||||||||||||||||||||| ||||||||
2960R-1.in silico 241 GATTCTGGCCCAGAAGTACAACTGCGACAAGATGATCTGCCGCAAGTGCTA-CGCCCGTC 300
2960R-1.IR_full 301 TGCATCCTCGTGCCACCAACTGCCGCAAGAAGAAGTGCGGACACACCAACAACCTGCGCC 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2960R-1.in silico 301 TGCATCCTCGTGCCACCAACTGCCGCAAGAAGAAGTGCGGACACACCAACAACCTGCGCC 360
2960R-1.IR_full 361 CCAAGAAGAAGTTGAAG 377
|||||||||||||||||
2960R-1.in silico 361 CCAAGAAGAAGTTGAAG 377
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
356 |
0 |
0 |
0 |
NM_057428.3 |
CG2960-RA (RpL40), mRNA |
| 68.25 |
243 |
363 |
621 |
449 |
NM_168043.2 |
CG11624-RA, transcript variant A (Ubi-p63E), mRNA |
| 68.25 |
243 |
363 |
621 |
449 |
NM_206272.1 |
CG11624-RC, transcript variant C (Ubi-p63E), mRNA |
| 68.25 |
243 |
363 |
621 |
449 |
NM_079185.4 |
CG11624-RB, transcript variant B (Ubi-p63E), mRNA |
| 13.2 |
47 |
323 |
477 |
311 |
NM_167065.1 |
CG32744-RA (CG32744), mRNA |
| 8.98 |
32 |
122 |
147 |
225 |
NM_132078.1 |
CG11700-RA (CG11700), mRNA |
| 5.89 |
21 |
43 |
38 |
41 |
NM_057430.3 |
CG5271-RA (RpS27A), mRNA |
| 0 |
0 |
2 |
5 |
2 |
NM_137762.2 |
CG9878-RA, transcript variant A (Tim10), mRNA |
| 0 |
0 |
2 |
5 |
2 |
NM_166474.1 |
CG9878-RB, transcript variant B (Tim10), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_132423.1 |
CG11556-RA (Rph), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_132658.1 |
CG1681-RA (CG1681), mRNA |
| 0 |
0 |
1 |
4 |
5 |
NM_142931.3 |
CG10192-RA (CG10192), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_141045.1 |
CG7632-RA (CG7632), mRNA |
| 0 |
0 |
1 |
3 |
7 |
NM_143283.1 |
CG5882-RA (CG5882), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_078519.1 |
CG1430-RA (bys), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_142577.1 |
CG4583-RA (ire-1), mRNA |
| 0 |
0 |
1 |
2 |
7 |
NM_139482.1 |
CG14950-RA (CG14950), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_141693.2 |
CG8507-RA (CG8507), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_136623.1 |
CG8014-RA (Rme-8), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_141570.2 |
CG9801-RA, transcript variant A (CG9801), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_206462.1 |
CG9801-RC, transcript variant C (CG9801), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_169239.1 |
CG9801-RB, transcript variant B (CG9801), mRNA |
| 0 |
0 |
0 |
7 |
6 |
NM_139981.2 |
CG6831-RA (rhea), mRNA |
| 0 |
0 |
0 |
7 |
2 |
NM_139392.1 |
CG7991-RA (CG7991), mRNA |
| 0 |
0 |
0 |
5 |
7 |
NM_140320.1 |
CG10663-RA (CG10663), mRNA |
| 0 |
0 |
0 |
5 |
7 |
NM_079461.2 |
CG5219-RA (mRpL15), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_130658.2 |
CG10260-RB (CG10260), mRNA |
| 0 |
0 |
0 |
4 |
14 |
NM_136075.1 |
CG10679-RA (Nedd8), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_167846.1 |
CG1086-RA, transcript variant A (Glut1), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_140063.3 |
CG3434-RA (CG3434), mRNA |
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