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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
2950R-1 |
| Symbol |
CG2950 |
Full Name |
CG2950 |
| CG No |
CG2950 |
Old CG No |
CG2950 |
| Synonyms |
cg2950, BcDNA:GH06479, CG2950 |
| Accession No (Link to NCBI) |
NM_164598.1 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
semi-lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgCGCCCGTTGAAGACACAATCG |
| Primer Seq. 3' |
aatctagaggtaccGCTGCAGATGCTGCATTGAAG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 CGCCCGTTGA AGACACAATC GCGATCGTCG TTGAAGAACA GCTACCTGGT GATCGAGGAG
0061 CTAATCCAGT TGATAGACAA CGTGACCGTC GGTCTTCAGT CGTGCAACAC CACGCCGGAA
0121 TCGATCACGC TGCTCCTGCA CAACCTCCGG GTGCATGGAC CGCAACTGGA GGCGGTGTCC
0181 AAGGATACAC TGGACCGGGC CTTCGTCGTT TTCCGCAACG CTTCGCAGGA CGAACGGCTG
0241 AATATAACGA CGCGCCTCAA GCTGCTCGAA CTCATCGAgC TGCGCGCCAA GAGCTGGGAC
0301 AATGACGACA CAATCGCGTA CTACAAGTCC AAGCAGCAGA TCTCCAACGT GGAGCTGCCT
0361 TCGGAATACC AGTACGATGC CGGCGTGCAG cCTGGCGCCT TCAGCACCTC CCCCACATTC
0421 GGCGTCAGCG GCGGTGTTGG TGGCGTTAAC GATGGAGCCG CAGCCGCcgC TGCCGCCGTC
0481 TTCAATGCAG CATCTGCAGC |
| in silico PCR Fragment |
0001 CGCCCGTTGA AGACACAATC GCGATCGTCG TTGAAGAACA GCTACCTGGT GATCGAGGAG
0061 CTAATCCAGT TGATAGACAA CGTGACCGTC GGTCTTCAGT CGTGCAACAC CACGCCGGAA
0121 TCGATCACGC TGCTCCTGCA CAACCTCCGG GTGCATGGAC CGCAACTGGA GGCGGTGTCC
0181 AAGGATACAC TGGACCGGGC CTTCGTCGTT TTCCGCAACG CTTCGCAGGA CGAACGGCTG
0241 AATATAACGA CGCGCCTCAA GCTGCTCGAA CTCATCGAGC TGCGCGCCAA GAGCTGGGAC
0301 AATGACGACA CAATCGCGTA CTACAAGTCC AAGCAGCAGA TCTCCAACGT GGAGCTGCCT
0361 TCGGAATACC AGTACGATGC CGGCGTGCAG CCTGGCGCCT TCAGCACCTC CCCCACATTC
0421 GGCGTCAGCG GCGGTGTTGG TGGCGTTAAC GATGGAGCCG CAGCCGCCGC TGCCGCCGTC
0481 TTCAATGCAG CATCTGCAGC |
| Assemble Data |
2950R-1.IR_full 1 CGCCCGTTGAAGACACAATCGCGATCGTCGTTGAAGAACAGCTACCTGGTGATCGAGGAG 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2950R-1.in silico 1 CGCCCGTTGAAGACACAATCGCGATCGTCGTTGAAGAACAGCTACCTGGTGATCGAGGAG 60
2950R-1.IR_full 61 CTAATCCAGTTGATAGACAACGTGACCGTCGGTCTTCAGTCGTGCAACACCACGCCGGAA 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2950R-1.in silico 61 CTAATCCAGTTGATAGACAACGTGACCGTCGGTCTTCAGTCGTGCAACACCACGCCGGAA 120
2950R-1.IR_full 121 TCGATCACGCTGCTCCTGCACAACCTCCGGGTGCATGGACCGCAACTGGAGGCGGTGTCC 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2950R-1.in silico 121 TCGATCACGCTGCTCCTGCACAACCTCCGGGTGCATGGACCGCAACTGGAGGCGGTGTCC 180
2950R-1.IR_full 181 AAGGATACACTGGACCGGGCCTTCGTCGTTTTCCGCAACGCTTCGCAGGACGAACGGCTG 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2950R-1.in silico 181 AAGGATACACTGGACCGGGCCTTCGTCGTTTTCCGCAACGCTTCGCAGGACGAACGGCTG 240
2950R-1.IR_full 241 AATATAACGACGCGCCTCAAGCTGCTCGAACTCATCGAGCTGCGCGCCAAGAGCTGGGAC 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2950R-1.in silico 241 AATATAACGACGCGCCTCAAGCTGCTCGAACTCATCGAGCTGCGCGCCAAGAGCTGGGAC 300
2950R-1.IR_full 301 AATGACGACACAATCGCGTACTACAAGTCCAAGCAGCAGATCTCCAACGTGGAGCTGCCT 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2950R-1.in silico 301 AATGACGACACAATCGCGTACTACAAGTCCAAGCAGCAGATCTCCAACGTGGAGCTGCCT 360
2950R-1.IR_full 361 TCGGAATACCAGTACGATGCCGGCGTGCAGCCTGGCGCCTTCAGCACCTCCCCCACATTC 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2950R-1.in silico 361 TCGGAATACCAGTACGATGCCGGCGTGCAGCCTGGCGCCTTCAGCACCTCCCCCACATTC 420
2950R-1.IR_full 421 GGCGTCAGCGGCGGTGTTGGTGGCGTTAACGATGGAGCCGCAGCCGCCGCTGCCGCCGTC 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2950R-1.in silico 421 GGCGTCAGCGGCGGTGTTGGTGGCGTTAACGATGGAGCCGCAGCCGCCGCTGCCGCCGTC 480
2950R-1.IR_full 481 TTCAATGCAGCATCTGCAGC 500
||||||||||||||||||||
2950R-1.in silico 481 TTCAATGCAGCATCTGCAGC 500
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
1 |
10 |
NM_164598.1 |
CG2950-RA, transcript variant A (CG2950), mRNA |
| 100 |
482 |
0 |
1 |
8 |
NM_135022.1 |
CG2950-RB, transcript variant B (CG2950), mRNA |
| 100 |
482 |
0 |
1 |
8 |
NM_164599.1 |
CG2950-RC, transcript variant C (CG2950), mRNA |
| 0.2 |
1 |
5 |
3 |
16 |
NM_130578.2 |
CG32809-RD, transcript variant D (CG32809), mRNA |
| 0.2 |
1 |
4 |
5 |
22 |
NM_166896.1 |
CG11491-RB, transcript variant B (br), mRNA |
| 0.2 |
1 |
4 |
5 |
22 |
NM_166897.1 |
CG11491-RC, transcript variant C (br), mRNA |
| 0.2 |
1 |
4 |
2 |
6 |
NM_079757.3 |
CG5610-RA (nAcRalpha-96Aa), mRNA |
| 0.2 |
1 |
2 |
8 |
11 |
NM_001014652.1 |
CG10693-RP, transcript variant P (slo), mRNA |
| 0.2 |
1 |
2 |
8 |
11 |
NM_001014651.1 |
CG10693-RQ, transcript variant Q (slo), mRNA |
| 0.2 |
1 |
2 |
8 |
11 |
NM_001014663.1 |
CG10693-RE, transcript variant E (slo), mRNA |
| 0.2 |
1 |
2 |
8 |
11 |
NM_001014664.1 |
CG10693-RD, transcript variant D (slo), mRNA |
| 0.2 |
1 |
2 |
8 |
11 |
NM_001014653.1 |
CG10693-RO, transcript variant O (slo), mRNA |
| 0.2 |
1 |
2 |
8 |
11 |
NM_001014660.1 |
CG10693-RH, transcript variant H (slo), mRNA |
| 0.2 |
1 |
2 |
8 |
11 |
NM_001014658.1 |
CG10693-RJ, transcript variant J (slo), mRNA |
| 0.2 |
1 |
2 |
8 |
11 |
NM_001014659.1 |
CG10693-RI, transcript variant I (slo), mRNA |
| 0.2 |
1 |
2 |
8 |
11 |
NM_001014656.1 |
CG10693-RL, transcript variant L (slo), mRNA |
| 0.2 |
1 |
2 |
8 |
11 |
NM_001014657.1 |
CG10693-RK, transcript variant K (slo), mRNA |
| 0.2 |
1 |
2 |
8 |
11 |
NM_079762.2 |
CG10693-RA, transcript variant A (slo), mRNA |
| 0.2 |
1 |
2 |
8 |
11 |
NM_001014661.1 |
CG10693-RG, transcript variant G (slo), mRNA |
| 0.2 |
1 |
2 |
8 |
11 |
NM_001014655.1 |
CG10693-RM, transcript variant M (slo), mRNA |
| 0.2 |
1 |
2 |
8 |
11 |
NM_001014654.1 |
CG10693-RN, transcript variant N (slo), mRNA |
| 0.2 |
1 |
2 |
8 |
11 |
NM_001014662.1 |
CG10693-RF, transcript variant F (slo), mRNA |
| 0.2 |
1 |
2 |
8 |
11 |
NM_206566.1 |
CG10693-RC, transcript variant C (slo), mRNA |
| 0.2 |
1 |
2 |
8 |
11 |
NM_170164.1 |
CG10693-RB, transcript variant B (slo), mRNA |
| 0 |
0 |
6 |
13 |
33 |
NM_001031888.1 |
CG33691-RB, transcript variant B (CG33691), mRNA |
| 0 |
0 |
6 |
13 |
33 |
NM_001031885.1 |
CG33692-RB, transcript variant B (CG33692), mRNA |
| 0 |
0 |
6 |
10 |
36 |
NM_001031889.1 |
CG33691-RA, transcript variant A (CG33691), mRNA |
| 0 |
0 |
6 |
10 |
36 |
NM_001031886.1 |
CG33692-RA, transcript variant A (CG33692), mRNA |
| 0 |
0 |
6 |
10 |
36 |
NM_001031887.1 |
CG33691-RC, transcript variant C (CG33691), mRNA |
| 0 |
0 |
6 |
8 |
24 |
NM_001031884.1 |
CG33692-RC, transcript variant C (CG33692), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
JNK signal
| N |
 |
| +- |
|
| DB |
 |
| + |
|
| DC |
 |
| ++ |
|
| DM |
 |
| + |
|
| VM |
 |
| |
|
| VB |
 |
| |
|
|
Caspase-3 signal
| N |
|
| + |
|
| DB |
|
| + |
|
| DC |
|
| ++ |
|
| DM |
|
| + |
|
| VM |
|
| |
|
| VB |
|
| |
|
|
|
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