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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 2909R-3 
 Symbol CG2909  Full Name CG2909 
 CG No CG2909  Old CG No CG2909 
 Synonyms CG2909 
 Accession No (Link to NCBI) NM_132388.2 
 Inserted Chr. ll 
 Insertional Mutation  lethal 
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624 [ PubMed ID = 21666802 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgGGTGATTCCCCGCATCTCACT 
 Primer Seq. 3' 
aatctagaggtaccAGAGCGGAGAGCTTGATATCG 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 GGTGATTCCC CGCATCTCAC TAATTTCGGA CGCAAATTGC TGAAGGATTG CCGCCAAGTG 
0061 CAGAAACCAA TCGGAGGATA CGAAAATTTG GGCAATGTGA TTAAATTGTC CGCGGAGTTT 
0121 CCGCTGGAGT TCGGCGTGAA CAGTGTCAAA GTATACCGGC AATCTCCATC GCGGCTTGCC 
0181 CGGATAAATG AGGAAGTGGC ATCCGCCTAT CCATTGATAC ACGAACGTAC ATTGGGCCTC 
0241 TACTTGCAGT ACTTGGAGCA CAAGTGTCGT TGGGGCAACG CGGTGGAAAA GCCCATCTAC 
0301 CGGAATTTAT CGCTATGCGG ATTTGTGCAA CGTCTGCTGG TAAAGCGTTG CGCCAGCTTC 
0361 TTTGCGCGGA ACGACAAGTA CCTGCTGGTG AGCGGAGAGT CCGGGGCCAG TGGCTTCGAA 
0421 GCCGTGGGTA CTCGGGAGGA GAAGGCGCCT CTGGTGCTGG CCAACGTGCT CAGCTATGAC 
0481 GATATCAAGC TCTCCGCTCT  
 in silico PCR Fragment 
0001 GGTGATTCCC CGCATCTCAC TAATTTCGGA CGCAAATTGC TGAAGGATTG CCGCCAAGTG 
0061 CAGAAACCAA TCGGAGGATA CGAAAATTTG GGCAATGTGA TTAAATTGTC CGCGGAGTTT 
0121 CCGCTGGAGT TCGGCGTGAA CAGTGTCAAA GTATACCGGC AATCTCCATC GCGGCTTGCC 
0181 CGGATAAATG AGGAAGTGGC ATCCGCCTAT CCATTGATAC ACGAACGTAC ATTGGGCCTC 
0241 TACTTGCAGT ACTTGGAGCA CAAGTGTCGT TGGGGCAACG CGGTGGAAAA GCCCATCTAC 
0301 CGGAATTTAT CGCTATGCGG ATTTGTGCAA CGTCTGCTGG TAAAGCGTTG CGCCAGCTTC 
0361 TTTGCGCGGA ACGACAAGTA CCTGCTGGTG AGCGGAGAGT CCGGGGCCAG TGGCTTCGAA 
0421 GCCGTGGGTA CTCGGGAGGA GAAGGCGCCT CTGGTGCTGG CCAACGTGCT CAGCTATGAC 
0481 GATATCAAGC TCTCCGCTCT  
 Assemble Data 

2909R-3.IR_full       1   GGTGATTCCCCGCATCTCACTAATTTCGGACGCAAATTGCTGAAGGATTGCCGCCAAGTG 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2909R-3.in silico     1   GGTGATTCCCCGCATCTCACTAATTTCGGACGCAAATTGCTGAAGGATTGCCGCCAAGTG 60


2909R-3.IR_full       61  CAGAAACCAATCGGAGGATACGAAAATTTGGGCAATGTGATTAAATTGTCCGCGGAGTTT 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2909R-3.in silico     61  CAGAAACCAATCGGAGGATACGAAAATTTGGGCAATGTGATTAAATTGTCCGCGGAGTTT 120


2909R-3.IR_full       121 CCGCTGGAGTTCGGCGTGAACAGTGTCAAAGTATACCGGCAATCTCCATCGCGGCTTGCC 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2909R-3.in silico     121 CCGCTGGAGTTCGGCGTGAACAGTGTCAAAGTATACCGGCAATCTCCATCGCGGCTTGCC 180


2909R-3.IR_full       181 CGGATAAATGAGGAAGTGGCATCCGCCTATCCATTGATACACGAACGTACATTGGGCCTC 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2909R-3.in silico     181 CGGATAAATGAGGAAGTGGCATCCGCCTATCCATTGATACACGAACGTACATTGGGCCTC 240


2909R-3.IR_full       241 TACTTGCAGTACTTGGAGCACAAGTGTCGTTGGGGCAACGCGGTGGAAAAGCCCATCTAC 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2909R-3.in silico     241 TACTTGCAGTACTTGGAGCACAAGTGTCGTTGGGGCAACGCGGTGGAAAAGCCCATCTAC 300


2909R-3.IR_full       301 CGGAATTTATCGCTATGCGGATTTGTGCAACGTCTGCTGGTAAAGCGTTGCGCCAGCTTC 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2909R-3.in silico     301 CGGAATTTATCGCTATGCGGATTTGTGCAACGTCTGCTGGTAAAGCGTTGCGCCAGCTTC 360


2909R-3.IR_full       361 TTTGCGCGGAACGACAAGTACCTGCTGGTGAGCGGAGAGTCCGGGGCCAGTGGCTTCGAA 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2909R-3.in silico     361 TTTGCGCGGAACGACAAGTACCTGCTGGTGAGCGGAGAGTCCGGGGCCAGTGGCTTCGAA 420


2909R-3.IR_full       421 GCCGTGGGTACTCGGGAGGAGAAGGCGCCTCTGGTGCTGGCCAACGTGCTCAGCTATGAC 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2909R-3.in silico     421 GCCGTGGGTACTCGGGAGGAGAAGGCGCCTCTGGTGCTGGCCAACGTGCTCAGCTATGAC 480


2909R-3.IR_full       481 GATATCAAGCTCTCCGCTCT 500
                          ||||||||||||||||||||
2909R-3.in silico     481 GATATCAAGCTCTCCGCTCT 500



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_132388.2  CG2909-RA (CG2909), mRNA 
0   0  4  2  2  NM_137837.1  CG3927-RA (CG3927), mRNA 
0   0  4  2  2  NM_137838.1  CG3875-RA (CG3875), mRNA 
0   0  4  2  2  NM_206018.1  CG33317-RA (CG33317), mRNA 
0   0  4  2  2  NR_001677.1  CR33318, mRNA 
0   0  2  3  2  NM_137766.1  CG4021-RA (CG4021), mRNA 
0   0  1  5  3  NM_134916.3  CG9663-RA (CG9663), mRNA 
0   0  1  4  12  NM_168641.1  CG5942-RB, transcript variant B (brm), mRNA 
0   0  1  4  12  NM_080497.4  CG5942-RC, transcript variant C (brm), mRNA 
0   0  1  4  12  NM_168640.1  CG5942-RD, transcript variant D (brm), mRNA 
0   0  1  4  12  NM_080498.2  CG5942-RA, transcript variant A (brm), mRNA 
0   0  1  3  4  NM_135247.2  CG9138-RA (SP1070), mRNA 
0   0  1  3  3  NM_206224.1  CG32346-RC, transcript variant C (E(bx)), mRNA 
0   0  1  3  3  NM_167817.2  CG32346-RB, transcript variant B (E(bx)), mRNA 
0   0  1  3  3  NM_167819.2  CG32346-RA, transcript variant A (E(bx)), mRNA 
0   0  1  3  2  NM_136434.2  CG1669-RA (kappaB-Ras), mRNA 
0   0  1  2  5  NM_057250.3  CG3647-RA, transcript variant A (stc), mRNA 
0   0  1  2  5  NM_057251.3  CG3647-RB, transcript variant B (stc), mRNA 
0   0  1  2  3  NM_140769.1  CG5290-RA (CG5290), mRNA 
0   0  0  15  27  NM_131938.1  CG3568-RA (CG3568), mRNA 
0   0  0  6  7  NM_168479.1  CG5946-RA, transcript variant A (CG5946), mRNA 
0   0  0  6  7  NM_140255.2  CG5946-RB, transcript variant B (CG5946), mRNA 
0   0  0  5  2  NM_057891.2  CG3365-RB, transcript variant B (drongo), mRNA 
0   0  0  4  7  NM_133041.1  CG7135-RA (CG7135), mRNA 
0   0  0  4  3  NM_079860.2  CG12073-RA (5-HT7), mRNA 
0   0  0  4  3  NM_134483.2  CG14208-RB, transcript variant B (Tyler), mRNA 
0   0  0  4  3  NM_167671.1  CG14208-RA, transcript variant A (Tyler), mRNA 
0   0  0  4  3  NM_001031900.1  CG14209-RD, transcript variant D (Shawn), mRNA 
0   0  0  4  3  NM_001031902.1  CG14209-RB, transcript variant B (Shawn), mRNA 
0   0  0  4  3  NM_001031901.1  CG14209-RC, transcript variant C (Shawn), mRNA 

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