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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
2905R-3 |
| Symbol |
Nipped-A |
Full Name |
Nipped-A |
| CG No |
CG33554 |
Old CG No |
CG2905 |
| Synonyms |
dTra1, dmTRRAP, TRRAP, CG2905, tra1, 41Ah, Nipped[A], l(2)41Ah, l(2)EMS34-12, GroupV, Tra-1, CG33554, l(2)C123, CG10549, Tra1, Nipped-A, lethal(2)41Ah, Nipped |
| Accession No (Link to NCBI) |
NM_001014499.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Parsons LM, Grzeschik NA, Amaratunga K, Burke P, Quinn LM, Richardson HE.
A Kinome RNAi Screen in Drosophila Identifies Novel Genes Interacting with Lgl, aPKC, and Crb Cell Polarity Genes in Epithelial Tissues.
G3 (Bethesda) (2017) 7(8) 2497-2509
[
PubMed ID = 28611255
]
[
RRC reference
]
Nitta Y, Yamazaki D, Sugie A, Hiroi M, Tabata T.
DISCO Interacting Protein 2 regulates axonal bifurcation and guidance of Drosophila mushroom body neurons.
Dev Biol (2017) 421(2) 233-244
[
PubMed ID = 27908785
]
[
RRC reference
]
Swarup S, Pradhan-Sundd T, Verheyen EM.
Genome-wide identification of phospho-regulators of Wnt signaling in Drosophila.
Development (2015) 142(8) 1502-15
[
PubMed ID = 25852200
]
[
RRC reference
]
Mummery-Widmer JL, Yamazaki M, Stoeger T, Novatchkova M, Bhalerao S, Chen D, Dietzl G, Dickson BJ, Knoblich JA.
Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi.
Nature (2009) 458(7241) 987-92
[
PubMed ID = 19363474
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgTTGTCCCAAGGAAGCCGCTCA |
| Primer Seq. 3' |
aatctagaggtaccGCGTTGCCAGATGAATTTACC |
| Predicted Fragment Size |
501 |
| IR fragment full Seq |
0001 TTGTCCCAAG GAAGCCGCTC ATCTCAGAAA AGAACTTTTA ATTGCTGCCC GTCATATATT
0061 TGCTACAGAT TTACGTCAAA AATTTATTCC ATCAATCGAA CAATTGTTGG ATGAGGATTT
0121 GCTTATTGGA AAAGGAGTTA CATTGGATTC TATTCGGCCA TTGGCGTATT CAACTTTAGC
0181 TGACCTAGCT CACCACGTTC GCCAAAGCCT AAATATAGAC GTTTTAATAA AGGCAGTGAA
0241 TCTCTTTTCA AAAAATGTTC ATGATGAATC ACTTGCTGTG GGAATACAAA CTATGTCATG
0301 CAAGCTTTTG TTAAATCTAG TTGATTGTTT ACGTCATCAC AGTGAAACAG AACCACAAAG
0361 ATCAAAAGCT CTACTCTCAA AACTGTTAAA AGTGTTTGTA AAAAAATTTG AAACTATAGC
0421 TAAAATTCAG CTTCCATTAA TAATTCAAAA GTGCAAAGGG CACGCATTTT CTGGAGCATT
0481 GGTAAATTCA TTCTGGCNAC Gc |
| in silico PCR Fragment |
0001 TTGTCCCAAG GAAGCCGCTC ATCTCAGAAA AGAACTTTTA ATTGCTGCCC GTCATATATT
0061 TGCTACAGAT TTACGTCAAA AATTTATTCC ATCAATCGAA CAATTGTTTG ATGAGGATTT
0121 GCTTATTGGA AAAGGAGTTA CATTGGATTC TATTCGGCCA TTGGCGTATT CAACTTTAGC
0181 TGACCTAGCT CACCACGTTC GCCAAAGCCT AAATATAGAC GTTTTAATAA AGGCAGTGAA
0241 TCTCTTTTCA AAAAATGTTC ATGATGAATC ACTTGCTGTG GGAATACAAA CTATGTCATG
0301 CAAGCTTTTG TTAAATCTAG TTGATTGTTT ACGTCATCAC AGTGAAACAG AACCACAAAG
0361 ATCAAAAGCT CTACTCTCAA AACTGTTAAA AGTGTTTGTA AAAAAATTTG AAACTATAGC
0421 TAAAATTCAG CTTCCATTAA TAATTCAAAA GTGCAAAGGG CACGCATTTT CTGGAGCATT
0481 GGTAAATTCA -TCTGGCAAC GC |
| Assemble Data |
2905R-3.IR_full 1 TTGTCCCAAGGAAGCCGCTCATCTCAGAAAAGAACTTTTAATTGCTGCCCGTCATATATT 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2905R-3.in silico 1 TTGTCCCAAGGAAGCCGCTCATCTCAGAAAAGAACTTTTAATTGCTGCCCGTCATATATT 60
2905R-3.IR_full 61 TGCTACAGATTTACGTCAAAAATTTATTCCATCAATCGAACAATTGTTGGATGAGGATTT 120
|||||||||||||||||||||||||||||||||||||||||||||||| |||||||||||
2905R-3.in silico 61 TGCTACAGATTTACGTCAAAAATTTATTCCATCAATCGAACAATTGTTTGATGAGGATTT 120
2905R-3.IR_full 121 GCTTATTGGAAAAGGAGTTACATTGGATTCTATTCGGCCATTGGCGTATTCAACTTTAGC 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2905R-3.in silico 121 GCTTATTGGAAAAGGAGTTACATTGGATTCTATTCGGCCATTGGCGTATTCAACTTTAGC 180
2905R-3.IR_full 181 TGACCTAGCTCACCACGTTCGCCAAAGCCTAAATATAGACGTTTTAATAAAGGCAGTGAA 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2905R-3.in silico 181 TGACCTAGCTCACCACGTTCGCCAAAGCCTAAATATAGACGTTTTAATAAAGGCAGTGAA 240
2905R-3.IR_full 241 TCTCTTTTCAAAAAATGTTCATGATGAATCACTTGCTGTGGGAATACAAACTATGTCATG 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2905R-3.in silico 241 TCTCTTTTCAAAAAATGTTCATGATGAATCACTTGCTGTGGGAATACAAACTATGTCATG 300
2905R-3.IR_full 301 CAAGCTTTTGTTAAATCTAGTTGATTGTTTACGTCATCACAGTGAAACAGAACCACAAAG 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2905R-3.in silico 301 CAAGCTTTTGTTAAATCTAGTTGATTGTTTACGTCATCACAGTGAAACAGAACCACAAAG 360
2905R-3.IR_full 361 ATCAAAAGCTCTACTCTCAAAACTGTTAAAAGTGTTTGTAAAAAAATTTGAAACTATAGC 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2905R-3.in silico 361 ATCAAAAGCTCTACTCTCAAAACTGTTAAAAGTGTTTGTAAAAAAATTTGAAACTATAGC 420
2905R-3.IR_full 421 TAAAATTCAGCTTCCATTAATAATTCAAAAGTGCAAAGGGCACGCATTTTCTGGAGCATT 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2905R-3.in silico 421 TAAAATTCAGCTTCCATTAATAATTCAAAAGTGCAAAGGGCACGCATTTTCTGGAGCATT 480
2905R-3.IR_full 481 GGTAAATTCATTCTGGCNACGC 502
|||||||||| |||||| ||||
2905R-3.in silico 481 GGTAAATTCA-TCTGGCAACGC 502
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
483 |
0 |
0 |
8 |
NM_001014499.2 |
CG33554-RA, transcript variant A (Nipped-A), mRNA |
| 90.06 |
435 |
2 |
1 |
9 |
NM_001043006.1 |
CG33554-RB, transcript variant B (Nipped-A), mRNA |
| 76.19 |
368 |
1 |
1 |
7 |
NM_001043007.1 |
CG33554-RC, transcript variant C (Nipped-A), mRNA |
| 0 |
0 |
3 |
2 |
3 |
NM_080509.2 |
CG17299-RB, transcript variant B (SNF4Agamma), mRNA |
| 0 |
0 |
3 |
2 |
2 |
NM_079125.2 |
CG4634-RA (Nurf-38), mRNA |
| 0 |
0 |
2 |
2 |
7 |
NM_132092.2 |
CG3869-RA, transcript variant A (Marf), mRNA |
| 0 |
0 |
2 |
2 |
7 |
NM_206634.1 |
CG3869-RC, transcript variant C (Marf), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_134890.2 |
CG17265-RA (CG17265), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_135567.2 |
CG6187-RA (RluA-2), mRNA |
| 0 |
0 |
1 |
2 |
7 |
NM_176064.1 |
CG10658-RA (Os9), mRNA |
| 0 |
0 |
0 |
6 |
8 |
NM_001015505.1 |
CG17866-PA.3 (CG17866), mRNA |
| 0 |
0 |
0 |
5 |
7 |
NM_078589.2 |
CG1903-RB, transcript variant B (sno), mRNA |
| 0 |
0 |
0 |
5 |
6 |
NM_176556.1 |
CG33106-RA, transcript variant A (mask), mRNA |
| 0 |
0 |
0 |
5 |
6 |
NM_176557.1 |
CG33106-RB, transcript variant B (mask), mRNA |
| 0 |
0 |
0 |
5 |
6 |
NM_166790.2 |
CG32019-RA, transcript variant A (bt), mRNA |
| 0 |
0 |
0 |
5 |
6 |
NM_205877.1 |
CG32019-RD, transcript variant D (bt), mRNA |
| 0 |
0 |
0 |
5 |
6 |
NM_205876.1 |
CG32019-RC, transcript variant C (bt), mRNA |
| 0 |
0 |
0 |
5 |
6 |
NM_205875.1 |
CG32019-RE, transcript variant E (bt), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_169935.1 |
CG31191-RA (CG31191), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_142283.2 |
CG17565-RA (CG17565), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_135649.1 |
CG4738-RA (CG4738), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_167132.1 |
CG2175-RB, transcript variant B (dec-1), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_167133.1 |
CG2175-RC, transcript variant C (dec-1), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_078517.2 |
CG2175-RA, transcript variant A (dec-1), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_135650.2 |
CG6258-RA (RfC38), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_001015222.1 |
CG40351-PB.3 (CG40351), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_001015221.1 |
CG40351-PA.3 (CG40351), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_141297.1 |
CG2926-RA (CG2926), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_001031959.1 |
CG33687-RA (CG33687), mRNA |
| 0 |
0 |
0 |
3 |
10 |
NM_169414.1 |
CG17309-RB, transcript variant B (Csk), mRNA |
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