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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 2837R-6 
 Symbol CG2837  Full Name CG2837 
 CG No CG2837  Old CG No CG2837 
 Synonyms CG2837 
 Accession No (Link to NCBI) NM_164601.1 
 Inserted Chr. ll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgATCGGCCTGCTCCACACACAA 
 Primer Seq. 3' 
aatctagaggtaccTAGAGCAACTGCAGCTGCTTC 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 ATCGGCCTGC TCCACACACA AAATGCATCA GCTTTTATTG TGCCCCGGGA ACTGCCCTCC 
0061 ATCTTATCCA TAGTTTACTC CAATATACCA CCGATTAAAA AGGGAACTGA TTCCCGTTTG 
0121 GGCTTTGgCT TTCGCCTGGG CGAGCATGCG GACTTTCAGG TGATGGTGGA ACTGGGTCCC 
0181 CAGAAGGAGA CGCGTCCCAT CGGCGAGCCC AACCAGGATG ATCAATCGTT CAACAAGCGC 
0241 CAGGTGAGCC AGAGCGATCA GAAGGCTCTG GCCCGTCAAC TTTATCGCCA GCAGGTGATG 
0301 GAGGAGGCGG AGAGATTGAT GACGTCCACG GAGAGGAATG GGGCCAGCTG GCTGCAGGCC 
0361 TGGTCGAATG GCATGAAACC GCAGAAGCCC AAATCAAAGG ATCAGGCCAA GAAACCAGTG 
0421 AAAGAAAGTT CTTCAAGCAA TTATCAGAAT GCACAGGCAA CAGATCCCAC CAGTGCCATG 
0481 AAGCAGCTGC AGTTGCTCTA  
 in silico PCR Fragment 
0001 ATCGGCCTGC TCCACACACA AAATGCATCA GCTTTTATTG TGCCCCGGGA ACTGCCCTCC 
0061 ATCTTATCCA TAGTTTACTC CAATATACCA CCGATTAAAA AGGGAACTGA TTCCCGTTTG 
0121 GGCTTTGGCT TTCGCCTGGG CGAGCATGCG GACTTTCAGG TGATGGTGGA ACTGGGTCCC 
0181 CAGAAGGAGA CGCGTCCCAT CGGCGAGCCC AACCAGGATG ATCAATCGTT CAACAAGCGC 
0241 CAGGTGAGCC AGAGCGATCA GAAGGCTCTG GCCCGTCAAC TTTATCGCCA GCAGGTGATG 
0301 GAGGAGGCGG AGAGATTGAT GACGTCCACG GAGAGGAATG GGGCCAGCTG GCTGCAGGCC 
0361 TGGTCGAATG GCATGAAACC GCAGAAGCCC AAATCAAAGG ATCAGGCCAA GAAACCAGTG 
0421 AAAGAAAGTT CTTCAAGCAA TTATCAGAAT GCACAGGCAA CAGATCCCAC CAGTGCCATG 
0481 AAGCAGCTGC AGTTGCTCTA  
 Assemble Data 

2837R-6.IR_full       1   ATCGGCCTGCTCCACACACAAAATGCATCAGCTTTTATTGTGCCCCGGGAACTGCCCTCC 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2837R-6.in silico     1   ATCGGCCTGCTCCACACACAAAATGCATCAGCTTTTATTGTGCCCCGGGAACTGCCCTCC 60


2837R-6.IR_full       61  ATCTTATCCATAGTTTACTCCAATATACCACCGATTAAAAAGGGAACTGATTCCCGTTTG 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2837R-6.in silico     61  ATCTTATCCATAGTTTACTCCAATATACCACCGATTAAAAAGGGAACTGATTCCCGTTTG 120


2837R-6.IR_full       121 GGCTTTGGCTTTCGCCTGGGCGAGCATGCGGACTTTCAGGTGATGGTGGAACTGGGTCCC 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2837R-6.in silico     121 GGCTTTGGCTTTCGCCTGGGCGAGCATGCGGACTTTCAGGTGATGGTGGAACTGGGTCCC 180


2837R-6.IR_full       181 CAGAAGGAGACGCGTCCCATCGGCGAGCCCAACCAGGATGATCAATCGTTCAACAAGCGC 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2837R-6.in silico     181 CAGAAGGAGACGCGTCCCATCGGCGAGCCCAACCAGGATGATCAATCGTTCAACAAGCGC 240


2837R-6.IR_full       241 CAGGTGAGCCAGAGCGATCAGAAGGCTCTGGCCCGTCAACTTTATCGCCAGCAGGTGATG 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2837R-6.in silico     241 CAGGTGAGCCAGAGCGATCAGAAGGCTCTGGCCCGTCAACTTTATCGCCAGCAGGTGATG 300


2837R-6.IR_full       301 GAGGAGGCGGAGAGATTGATGACGTCCACGGAGAGGAATGGGGCCAGCTGGCTGCAGGCC 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2837R-6.in silico     301 GAGGAGGCGGAGAGATTGATGACGTCCACGGAGAGGAATGGGGCCAGCTGGCTGCAGGCC 360


2837R-6.IR_full       361 TGGTCGAATGGCATGAAACCGCAGAAGCCCAAATCAAAGGATCAGGCCAAGAAACCAGTG 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2837R-6.in silico     361 TGGTCGAATGGCATGAAACCGCAGAAGCCCAAATCAAAGGATCAGGCCAAGAAACCAGTG 420


2837R-6.IR_full       421 AAAGAAAGTTCTTCAAGCAATTATCAGAATGCACAGGCAACAGATCCCACCAGTGCCATG 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2837R-6.in silico     421 AAAGAAAGTTCTTCAAGCAATTATCAGAATGCACAGGCAACAGATCCCACCAGTGCCATG 480


2837R-6.IR_full       481 AAGCAGCTGCAGTTGCTCTA 500
                          ||||||||||||||||||||
2837R-6.in silico     481 AAGCAGCTGCAGTTGCTCTA 500



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_135030.2  CG2837-RB, transcript variant B (CG2837), mRNA 
100   482  0  0  0  NM_164602.1  CG2837-RC, transcript variant C (CG2837), mRNA 
100   482  0  0  0  NM_164601.1  CG2837-RA, transcript variant A (CG2837), mRNA 
0   0  4  3  3  NM_140647.2  CG4157-RA (Rpn12), mRNA 
0   0  3  5  2  NM_134550.1  CG1695-RA, transcript variant A (CG1695), mRNA 
0   0  3  5  2  NM_134551.4  CG32506-RA (CG32506), mRNA 
0   0  3  5  2  NM_167705.1  CG1695-RB, transcript variant B (CG1695), mRNA 
0   0  3  4  7  NM_132367.1  CG15252-RA (CG15252), mRNA 
0   0  3  4  4  NM_134990.2  CG15433-RA (CG15433), mRNA 
0   0  2  6  8  NM_167950.1  CG1146-RB, transcript variant B (CG1146), mRNA 
0   0  2  6  8  NM_167951.1  CG1146-RC, transcript variant C (CG1146), mRNA 
0   0  2  6  8  NM_139468.1  CG1146-RA, transcript variant A (CG1146), mRNA 
0   0  2  5  3  NM_167324.1  CG32653-RA (CG32653), mRNA 
0   0  2  4  6  NM_144381.1  CG15378-RA (lectin-22C), mRNA 
0   0  2  4  2  NM_078689.2  CG14228-RA (Mer), mRNA 
0   0  2  3  10  NM_176113.1  CG2049-RD, transcript variant D (Pkn), mRNA 
0   0  2  3  10  NM_176111.1  CG2049-RC, transcript variant C (Pkn), mRNA 
0   0  2  2  2  NM_001038797.1  CG34059-RA (ppk16), mRNA 
0   0  2  2  2  NM_135216.2  CG11070-RA (CG11070), mRNA 
0   0  1  4  3  NM_138257.2  CG2213-RA (CG2213), mRNA 
0   0  1  3  6  NM_137493.2  CG30116-RC, transcript variant C (CG30116), mRNA 
0   0  1  3  6  NM_137494.1  CG30116-RB, transcript variant B (CG30116), mRNA 
0   0  1  3  4  NM_140006.1  CG5751-RA (TrpA1), mRNA 
0   0  1  3  4  NM_166292.1  CG30116-RA, transcript variant A (CG30116), mRNA 
0   0  1  3  4  NM_166291.1  CG30116-RD, transcript variant D (CG30116), mRNA 
0   0  1  2  8  NM_165165.1  CG5953-RB, transcript variant B (CG5953), mRNA 
0   0  1  2  8  NM_135952.1  CG5953-RA, transcript variant A (CG5953), mRNA 
0   0  1  2  7  NM_165026.1  CG16975-RA, transcript variant A (CG16975), mRNA 
0   0  1  2  7  NM_143141.2  CG4548-RB, transcript variant B (XNP), mRNA 
0   0  1  2  7  NM_135762.2  CG16975-RB, transcript variant B (CG16975), mRNA 

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