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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 2788R-1 
 Symbol Dot  Full Name Dorothy 
 CG No CG2788  Old CG No CG2788 
 Synonyms CG2788, Dot 
 Accession No (Link to NCBI) NM_079947.2 
 Inserted Chr. ll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees pupal lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgCCTATCCCAACTATGAGGAGC 
 Primer Seq. 3' 
aatctagaggtaccCCGTACCAACTCGTTGTCAGC 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 CCTATCCCAA CTATGAGGAG CTACTGATTT CGGCTCCCGG TGAGGATCAG TCGAGCACCA 
0061 CGATCAACTT GGTTCCCCTG ACGGAGCACA CACCCACCAG ATCCCTGGGA GTCCTCATCC 
0121 GTGAGTATGT AGATCTGCAC GAAGAGGGCC AGAAGACCTG CGAGCACCTC TTCGCCAGTG 
0181 GCCACATAGA ACGGGCCATC GAACGACATC GAAATAAGCC CTACGATCTG CTACTCACGG 
0241 AATACTTCAA CTCCGATTGC CAACTGGCCT TGGCCAAGTT ATTAAATCTG CCAATTATAG 
0301 GCTTAAGTAC CTGCGCGCTG ATGCCCTACT ATTACGATCG CATCGATTTG CCCGACACAC 
0361 CTGCCTTCAT ACAGTCGGAA TTCGTGGGTT TCGCTGGCCA ACTGAACTGG CACGAACGGC 
0421 TGCTCAACTT TGTTCAGGCC AAGTTATTAA AGTTTCTGTA CAAGTACCAT TCCAACAGGG 
0481 CTGACAACGA GTTGGTACGG  
 in silico PCR Fragment 
0001 CCTATCCCAA CTATGAGGAG CTACTGATTT CGGCTCCCGG TGAGGATCAG TCGAGCACCA 
0061 CGATCAACTT GGTTCCCCTG ACGGAGCACA CACCCACCAG ATCCCTGGGA GTCCTCATCC 
0121 GTGAGTATGT AGATCTGCAC GAAGAGGGCC AGAAGACCTG CGAGCACCTC TTCGCCAGTG 
0181 GCCACATAGA ACGGGCCATC GAACGACATC GAAATAAGCC CTACGATCTG CTACTCACGG 
0241 AATACTTCAA CTCCGATTGC CAACTGGCCT TGGCCAAGTT ATTAAATCTG CCAATTATAG 
0301 GCTTAAGTAC CTGCGCGCTG ATGCCCTACT ATTACGATCG CATCGATTTG CCCGACACAC 
0361 CTGCCTTCAT ACAGTCGGAA TTCGTGGGTT TCGCTGGCCA ACTGAACTGG CACGAACGGC 
0421 TGCTCAACTT TGTTCAGGCC AAGTTATTAA AGTTTCTGTA CAAGTACCAT TCCAACAGGG 
0481 CTGACAACGA GTTGGTACGG  
 Assemble Data 

2788R-1.IR_full       1   CCTATCCCAACTATGAGGAGCTACTGATTTCGGCTCCCGGTGAGGATCAGTCGAGCACCA 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2788R-1.in silico     1   CCTATCCCAACTATGAGGAGCTACTGATTTCGGCTCCCGGTGAGGATCAGTCGAGCACCA 60


2788R-1.IR_full       61  CGATCAACTTGGTTCCCCTGACGGAGCACACACCCACCAGATCCCTGGGAGTCCTCATCC 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2788R-1.in silico     61  CGATCAACTTGGTTCCCCTGACGGAGCACACACCCACCAGATCCCTGGGAGTCCTCATCC 120


2788R-1.IR_full       121 GTGAGTATGTAGATCTGCACGAAGAGGGCCAGAAGACCTGCGAGCACCTCTTCGCCAGTG 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2788R-1.in silico     121 GTGAGTATGTAGATCTGCACGAAGAGGGCCAGAAGACCTGCGAGCACCTCTTCGCCAGTG 180


2788R-1.IR_full       181 GCCACATAGAACGGGCCATCGAACGACATCGAAATAAGCCCTACGATCTGCTACTCACGG 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2788R-1.in silico     181 GCCACATAGAACGGGCCATCGAACGACATCGAAATAAGCCCTACGATCTGCTACTCACGG 240


2788R-1.IR_full       241 AATACTTCAACTCCGATTGCCAACTGGCCTTGGCCAAGTTATTAAATCTGCCAATTATAG 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2788R-1.in silico     241 AATACTTCAACTCCGATTGCCAACTGGCCTTGGCCAAGTTATTAAATCTGCCAATTATAG 300


2788R-1.IR_full       301 GCTTAAGTACCTGCGCGCTGATGCCCTACTATTACGATCGCATCGATTTGCCCGACACAC 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2788R-1.in silico     301 GCTTAAGTACCTGCGCGCTGATGCCCTACTATTACGATCGCATCGATTTGCCCGACACAC 360


2788R-1.IR_full       361 CTGCCTTCATACAGTCGGAATTCGTGGGTTTCGCTGGCCAACTGAACTGGCACGAACGGC 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2788R-1.in silico     361 CTGCCTTCATACAGTCGGAATTCGTGGGTTTCGCTGGCCAACTGAACTGGCACGAACGGC 420


2788R-1.IR_full       421 TGCTCAACTTTGTTCAGGCCAAGTTATTAAAGTTTCTGTACAAGTACCATTCCAACAGGG 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2788R-1.in silico     421 TGCTCAACTTTGTTCAGGCCAAGTTATTAAAGTTTCTGTACAAGTACCATTCCAACAGGG 480


2788R-1.IR_full       481 CTGACAACGAGTTGGTACGG 500
                          ||||||||||||||||||||
2788R-1.in silico     481 CTGACAACGAGTTGGTACGG 500



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  6  NM_079947.2  CG2788-RA (Dot), mRNA 
0.41   2  2  2  3  NM_138200.2  CG12030-RA (CG12030), mRNA 
0   0  3  3  5  NM_132125.1  CG4536-RA (CG4536), mRNA 
0   0  2  3  2  NM_167562.1  CG10598-RB, transcript variant B (CG10598), mRNA 
0   0  1  4  3  NM_143520.1  CG15530-RA, transcript variant A (CG15530), mRNA 
0   0  1  4  3  NM_170475.1  CG15530-RB, transcript variant B (CG15530), mRNA 
0   0  1  3  3  NM_141114.2  CG7143-RA (DNApol-eta), mRNA 
0   0  1  3  2  NM_134544.1  CG11734-RB (HERC2), mRNA 
0   0  1  2  2  NM_141936.1  CG6525-RA (CG6525), mRNA 
0   0  0  7  4  NM_057598.3  CG10739-RA (pigeon), mRNA 
0   0  0  6  3  NM_175983.1  CG8222-RC, transcript variant C (Pvr), mRNA 
0   0  0  6  3  NM_205926.1  CG8222-RE, transcript variant E (Pvr), mRNA 
0   0  0  6  3  NM_205925.1  CG8222-RF, transcript variant F (Pvr), mRNA 
0   0  0  6  3  NM_078785.2  CG8222-RA, transcript variant A (Pvr), mRNA 
0   0  0  6  3  NM_164801.1  CG8222-RB, transcript variant B (Pvr), mRNA 
0   0  0  6  3  NM_205927.1  CG8222-RD, transcript variant D (Pvr), mRNA 
0   0  0  5  6  NM_135325.2  CG7227-RA (CG7227), mRNA 
0   0  0  5  3  NM_168464.1  CG32086-RA (CG32086), mRNA 
0   0  0  4  3  NM_165386.1  CG2201-RA, transcript variant A (CG2201), mRNA 
0   0  0  4  3  NM_142399.1  CG7379-RA (CG7379), mRNA 
0   0  0  4  3  NM_001038842.1  CG2201-RE, transcript variant E (CG2201), mRNA 
0   0  0  4  3  NM_001038843.1  CG2201-RD, transcript variant D (CG2201), mRNA 
0   0  0  4  3  NM_136271.2  CG2201-RC, transcript variant C (CG2201), mRNA 
0   0  0  4  3  NM_165387.1  CG2201-RB, transcript variant B (CG2201), mRNA 
0   0  0  4  3  NM_142755.1  CG5732-RA (CG5732), mRNA 
0   0  0  4  2  NM_176414.1  CG1988-RB, transcript variant B (CG1988), mRNA 
0   0  0  4  2  NM_141430.2  CG1988-RA, transcript variant A (CG1988), mRNA 
0   0  0  4  2  NM_078867.2  CG5526-RA (Dhc36C), mRNA 
0   0  0  3  7  NM_206741.1  CG16952-RB, transcript variant B (CG16952), mRNA 
0   0  0  3  7  NM_132857.2  CG16952-RA, transcript variant A (CG16952), mRNA 

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