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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 2714R-3 
 Symbol crm  Full Name cramped 
 CG No CG2714  Old CG No CG2714 
 Synonyms CG2714, EG:95B7.2, zw-9, swa, zw9, l(1)zw9, sa, ZW-9, l(1)3C1, sta[P], l(1)3Ca, crm 
 Accession No (Link to NCBI) NM_057757.3 
 Inserted Chr. ll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Cho Y, Lai CM, Lin KY, Hsu HJ.
A Targeted RNAi Screen Reveals Drosophila Female-Sterile Genes That Control the Size of Germline Stem Cell Niche During Development.
G3 (Bethesda) (2018) 8(7) 2345-2354 [ PubMed ID = 29764959 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgGTGTCCATTGAGGAGCCGCTG 
 Primer Seq. 3' 
aatctagaggtaccAACTCCTGGGCGGGCTTTTTC 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 GTGTCCATTG AGGAGCCGCT GCCAAATGGC AAGGGAGGAG GAGCAGTAGT TGTTAACTCG 
0061 ATAGCTAAGC TGCCCGAGGA GGAGCTCCTC GGCTCGGTGA CCATGCACAA TTGTCCGGGA 
0121 ACGCGGGCCA GTGCCCGGGT CNATACAGAA GATGAAGCAG GACCAGACGC GCCCGATGAC 
0181 ACCGCCGCCC AGCGAACGGG AGCCGAACAA AAAGGAGGAG AAGGCCGCCC AAAAGACNGC 
0241 CCAGCCAGCT GAAGACGGGC AGCGGGAAGA CCACCTGGAC GAATGTGGAG CGCAACTGCT 
0301 TCTTCGACGC CCTCAACGAA TTCGGCAAGG ACTTCGAgGC GGTGGCCAAC TGCATCAATG 
0361 CCAANNTGAA NCGCCgCAAT GCCAACAGCG ACTATAGTTT CAAAACCAAG GACCAAGTTC 
0421 GCCAGCACTA TTACCAGACC CATCACAAGA TTTGCAAATA TGTGCGCTTC TCGGAGGAAC 
0481 TGAAAAAGCC CGCCCAgGAG TT 
 in silico PCR Fragment 
0001 GTGTCCATTG AGGAGCCGCT GCCAAATGGC AAGGGAGGAG GAGCAGTAGT TGTTAACTCG 
0061 ATAGCTAAGC TGCCCGAGGA GGAGCTCCTC GGCTCGGTGA CCATGCACAA TTGTCCGGGA 
0121 ACGCGGGCCA GTGCCCGGGT C-ATACAGAA GATGAAGCAG GACCAGACGC GCCCGATGAC 
0181 ACCGCCGCCC AGCGAACGGG AGCCGAACAA AAAGGAGGAG AAGGCCGCCC AAAAGAC-GC 
0241 CCAGCCAGCT GAAGACGGGC AGCGGGAAGA CCACCTGGAC GAATGTGGAG CGCAACTGCT 
0301 TCTTCGACGC CCTCAACGAA TTCGGCAAGG ACTTCGAGGC GGTGGCCAAC TGCATCAATG 
0361 CCAAGCTGAA GCGCCGCAAT GCCAACAGCG ACTATAGTTT CAAAACCAAG GACCAAGTTC 
0421 GCCAGCACTA TTACCAGACC CATCACAAGA TTTGCAAATA TGTGCGCTTC TCGGAGGAAC 
0481 TGAAAAAGCC CGCCCAGGAG TT 
 Assemble Data 

2714R-3.IR_full       1   GTGTCCATTGAGGAGCCGCTGCCAAATGGCAAGGGAGGAGGAGCAGTAGTTGTTAACTCG 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2714R-3.in silico     1   GTGTCCATTGAGGAGCCGCTGCCAAATGGCAAGGGAGGAGGAGCAGTAGTTGTTAACTCG 60


2714R-3.IR_full       61  ATAGCTAAGCTGCCCGAGGAGGAGCTCCTCGGCTCGGTGACCATGCACAATTGTCCGGGA 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2714R-3.in silico     61  ATAGCTAAGCTGCCCGAGGAGGAGCTCCTCGGCTCGGTGACCATGCACAATTGTCCGGGA 120


2714R-3.IR_full       121 ACGCGGGCCAGTGCCCGGGTCNATACAGAAGATGAAGCAGGACCAGACGCGCCCGATGAC 180
                          ||||||||||||||||||||| ||||||||||||||||||||||||||||||||||||||
2714R-3.in silico     121 ACGCGGGCCAGTGCCCGGGTC-ATACAGAAGATGAAGCAGGACCAGACGCGCCCGATGAC 180


2714R-3.IR_full       181 ACCGCCGCCCAGCGAACGGGAGCCGAACAAAAAGGAGGAGAAGGCCGCCCAAAAGACNGC 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||| ||
2714R-3.in silico     181 ACCGCCGCCCAGCGAACGGGAGCCGAACAAAAAGGAGGAGAAGGCCGCCCAAAAGAC-GC 240


2714R-3.IR_full       241 CCAGCCAGCTGAAGACGGGCAGCGGGAAGACCACCTGGACGAATGTGGAGCGCAACTGCT 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2714R-3.in silico     241 CCAGCCAGCTGAAGACGGGCAGCGGGAAGACCACCTGGACGAATGTGGAGCGCAACTGCT 300


2714R-3.IR_full       301 TCTTCGACGCCCTCAACGAATTCGGCAAGGACTTCGAGGCGGTGGCCAACTGCATCAATG 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2714R-3.in silico     301 TCTTCGACGCCCTCAACGAATTCGGCAAGGACTTCGAGGCGGTGGCCAACTGCATCAATG 360


2714R-3.IR_full       361 CCAANNTGAANCGCCGCAATGCCAACAGCGACTATAGTTTCAAAACCAAGGACCAAGTTC 420
                          ||||  |||| |||||||||||||||||||||||||||||||||||||||||||||||||
2714R-3.in silico     361 CCAAGCTGAAGCGCCGCAATGCCAACAGCGACTATAGTTTCAAAACCAAGGACCAAGTTC 420


2714R-3.IR_full       421 GCCAGCACTATTACCAGACCCATCACAAGATTTGCAAATATGTGCGCTTCTCGGAGGAAC 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2714R-3.in silico     421 GCCAGCACTATTACCAGACCCATCACAAGATTTGCAAATATGTGCGCTTCTCGGAGGAAC 480


2714R-3.IR_full       481 TGAAAAAGCCCGCCCAGGAGTT 502
                          ||||||||||||||||||||||
2714R-3.in silico     481 TGAAAAAGCCCGCCCAGGAGTT 502



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  1  NM_166951.1  CG2714-RB, transcript variant B (crm), mRNA 
100   482  0  0  1  NM_057757.3  CG2714-RA, transcript variant A (crm), mRNA 
0.41   2  3  3  6  NM_176437.1  CG9423-RC, transcript variant C (Kap-alpha3), mRNA 
0.41   2  3  3  6  NM_169296.2  CG9423-RB, transcript variant B (Kap-alpha3), mRNA 
0.41   2  3  3  6  NM_169295.2  CG9423-RA, transcript variant A (Kap-alpha3), mRNA 
0.2   1  2  2  4  NM_134924.1  CG3327-RB, transcript variant B (E23), mRNA 
0.2   1  2  2  4  NM_079909.2  CG3327-RA, transcript variant A (E23), mRNA 
0.2   1  2  2  4  NM_205900.1  CG3327-RC, transcript variant C (E23), mRNA 
0   0  3  4  4  NM_169947.1  CG17299-RF, transcript variant F (SNF4Agamma), mRNA 
0   0  3  2  5  NM_165255.1  CG10283-RB, transcript variant B (CG10283), mRNA 
0   0  3  2  5  NM_136035.4  CG10283-RA, transcript variant A (CG10283), mRNA 
0   0  2  4  6  NM_130516.2  CG3704-RA (CG3704), mRNA 
0   0  2  4  4  NM_057260.3  CG10561-RA (CG10561), mRNA 
0   0  2  2  8  NM_169637.2  CG4898-RF, transcript variant F (Tm1), mRNA 
0   0  2  2  4  NM_080112.1  CG1857-RA (nec), mRNA 
0   0  2  2  2  NM_164651.1  CG14011-RB, transcript variant B (CG14011), mRNA 
0   0  2  2  2  NM_135099.1  CG14011-RA, transcript variant A (CG14011), mRNA 
0   0  2  2  2  NM_164652.1  CG14011-RC, transcript variant C (CG14011), mRNA 
0   0  2  2  2  NM_140195.2  CG6190-RA (As), mRNA 
0   0  2  1  1  NM_166904.1  CG32806-RA, transcript variant A (CG32806), mRNA 
0   0  1  5  2  NM_169833.1  CG7717-RB, transcript variant B (Mekk1), mRNA 
0   0  1  5  2  NM_142493.2  CG7717-RA, transcript variant A (Mekk1), mRNA 
0   0  1  4  5  NM_132855.2  CG9038-RA, transcript variant A (UBL3), mRNA 
0   0  1  4  5  NM_206730.1  CG9038-RB, transcript variant B (UBL3), mRNA 
0   0  1  4  4  NM_079675.2  CG5452-RA (dnk), mRNA 
0   0  1  4  3  NM_132719.2  CG32604-RA, transcript variant A (l(1)G0007), mRNA 
0   0  1  4  2  NM_132329.3  CG16892-RA (CG16892), mRNA 
0   0  1  3  6  NM_001042852.1  CG12500-RB, transcript variant B (stnA), mRNA 
0   0  1  3  6  NM_001042851.1  CG12500-RA, transcript variant A (stnA), mRNA 
0   0  1  3  6  NM_001042854.1  CG12473-RB, transcript variant B (stnB), mRNA 

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