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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
2615R-2 |
| Symbol |
ik2 |
Full Name |
IkappaB kinase-like 2 |
| CG No |
CG2615 |
Old CG No |
CG2615 |
| Synonyms |
ik2, l(2)38Ea, DmIKKepsilon, APTX7, CG2615, IKKepsilon, Ik2, Ik2/DmIKK, dIKK, IK2, 38D.31, DmIKKepsilon/dIK2, Dmik2, DIK2, dik2, Dik2 |
| Accession No (Link to NCBI) |
NM_136204.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
semi-lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Parsons LM, Grzeschik NA, Amaratunga K, Burke P, Quinn LM, Richardson HE.
A Kinome RNAi Screen in Drosophila Identifies Novel Genes Interacting with Lgl, aPKC, and Crb Cell Polarity Genes in Epithelial Tissues.
G3 (Bethesda) (2017) 7(8) 2497-2509
[
PubMed ID = 28611255
]
[
RRC reference
]
Oshima K, Takeda M, Kuranaga E, Ueda R, Aigaki T, Miura M, Hayashi S.
IKK epsilon regulates F actin assembly and interacts with Drosophila IAP1 in cellular morphogenesis.
Curr Biol (2006) 16(15) 1531-7
[
PubMed ID = 16887350
]
[
RRC reference
]
|
| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGTGTTCCAGGGGGTCAACAAG |
| Primer Seq. 3' |
aatctagaggtaccACAGCGCGCTCGTAGAGATCG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 NTGTTCCAGG GGGTCAACAA GATCACCGGC GAATCGGTGG CGGTGAAGAC CTTTAATCCC
0061 TACAGTCACA TGCGACCGGC TGATGTGCAG ATGCGGGAGT TCGAGGCCCT GAAGAAGGTC
0121 AACCACGAGA ATATAGTAAA GCTGTTGGCG ATCGAGGAGG ATCAAGAGGG GCGTGGTAAG
0181 GTGATCGTGA TGGAGCTCTG CACAGGCGGA AGTCTCTTTA ACATCCTGGA CGATCCTGAG
0241 AACTCGTACG GTCTGCCGGA ACACGAGTTC CTGCTGGTCT TGGAACACTT GTGCGCCGGA
0301 ATGAAGCACT TGCGGGATAA CAANGCTGGT GCATCGCGAT CTGAAGCCCG GAAACATAAT
0361 GAAGTTCATC TCGGAGGACG GGCAAACCAT ATACAAGCTT ACTGATTTCG GTGCTGCTAG
0421 AGAACTGGAG GATAATCAGC CGTTTGCCTC TCTATACGGC ACAGAAGAGT ATCTTCATCC
0481 CGATCTCTAC GAGCGCGCTG T |
| in silico PCR Fragment |
0001 GTGTTCCAGG GGGTCAACAA GATCACCGGC GAATCGGTGG CGGTGAAGAC CTTTAATCCC
0061 TACAGTCACA TGCGACCGGC TGATGTGCAG ATGCGGGAGT TCGAGGCCCT GAAAAAGGTC
0121 AACCACGAGA ATATAGTAAA GCTGTTGGCG ATCGAGGAGG ATCAAGAGGG GCGTGGTAAG
0181 GTGATCGTGA TGGAGCTCTG CACAGGCGGA AGTCTCTTTA ACATCCTGGA CGATCCTGAG
0241 AACTCGTACG GTCTGCCGGA ACACGAGTTC CTGCTGGTCT TGGAACACTT GTGCGCCGGA
0301 ATGAAGCACT TGCGGGATAA CAA-GCTGGT GCATCGCGAT CTGAAGCCCG GAAACATAAT
0361 GAAGTTCATC TCGGAGGACG GGCAAACCAT ATACAAGCTT ACTGATTTCG GTGCTGCTAG
0421 AGAACTGGAG GATAATCAGC CGTTTGCCTC TCTATACGGC ACAGAAGAGT ATCTTCATCC
0481 CGATCTCTAC GAGCGCGCTG T |
| Assemble Data |
2615R-2.IR_full 1 TGTTCCAGGGGGTCAACAAGATCACCGGCGAATCGGTGGCGGTGAAGACCTTTAATCCC 59
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2615R-2.in silico 1 TGTTCCAGGGGGTCAACAAGATCACCGGCGAATCGGTGGCGGTGAAGACCTTTAATCCC 59
2615R-2.IR_full 61 TACAGTCACATGCGACCGGCTGATGTGCAGATGCGGGAGTTCGAGGCCCTGAAGAAGGTC 119
||||||||||||||||||||||||||||||||||||||||||||||||||||| ||||||
2615R-2.in silico 61 TACAGTCACATGCGACCGGCTGATGTGCAGATGCGGGAGTTCGAGGCCCTGAAAAAGGTC 119
2615R-2.IR_full 121 AACCACGAGAATATAGTAAAGCTGTTGGCGATCGAGGAGGATCAAGAGGGGCGTGGTAAG 179
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2615R-2.in silico 121 AACCACGAGAATATAGTAAAGCTGTTGGCGATCGAGGAGGATCAAGAGGGGCGTGGTAAG 179
2615R-2.IR_full 181 GTGATCGTGATGGAGCTCTGCACAGGCGGAAGTCTCTTTAACATCCTGGACGATCCTGAG 239
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2615R-2.in silico 181 GTGATCGTGATGGAGCTCTGCACAGGCGGAAGTCTCTTTAACATCCTGGACGATCCTGAG 239
2615R-2.IR_full 241 AACTCGTACGGTCTGCCGGAACACGAGTTCCTGCTGGTCTTGGAACACTTGTGCGCCGGA 299
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2615R-2.in silico 241 AACTCGTACGGTCTGCCGGAACACGAGTTCCTGCTGGTCTTGGAACACTTGTGCGCCGGA 299
2615R-2.IR_full 301 ATGAAGCACTTGCGGGATAACAANGCTGGTGCATCGCGATCTGAAGCCCGGAAACATAAT 359
||||||||||||||||||||||| ||||||||||||||||||||||||||||||||||||
2615R-2.in silico 301 ATGAAGCACTTGCGGGATAACAA-GCTGGTGCATCGCGATCTGAAGCCCGGAAACATAAT 359
2615R-2.IR_full 361 GAAGTTCATCTCGGAGGACGGGCAAACCATATACAAGCTTACTGATTTCGGTGCTGCTAG 419
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2615R-2.in silico 361 GAAGTTCATCTCGGAGGACGGGCAAACCATATACAAGCTTACTGATTTCGGTGCTGCTAG 419
2615R-2.IR_full 421 AGAACTGGAGGATAATCAGCCGTTTGCCTCTCTATACGGCACAGAAGAGTATCTTCATCC 479
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2615R-2.in silico 421 AGAACTGGAGGATAATCAGCCGTTTGCCTCTCTATACGGCACAGAAGAGTATCTTCATCC 479
2615R-2.IR_full 481 CGATCTCTACGAGCGCGCTGT 500
|||||||||||||||||||||
2615R-2.in silico 481 CGATCTCTACGAGCGCGCTGT 500
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_165337.1 |
CG2615-RB, transcript variant B (ik2), mRNA |
| 100 |
482 |
0 |
0 |
0 |
NM_136204.2 |
CG2615-RA, transcript variant A (ik2), mRNA |
| 0.2 |
1 |
2 |
2 |
2 |
NM_167015.2 |
CG6998-RC, transcript variant C (ctp), mRNA |
| 0.2 |
1 |
2 |
2 |
2 |
NM_080336.3 |
CG6998-RA, transcript variant A (ctp), mRNA |
| 0.2 |
1 |
2 |
2 |
2 |
NM_167016.1 |
CG6998-RD, transcript variant D (ctp), mRNA |
| 0.2 |
1 |
2 |
2 |
2 |
NM_167014.1 |
CG6998-RB, transcript variant B (ctp), mRNA |
| 0 |
0 |
4 |
3 |
11 |
NM_166125.2 |
CG18255-RA, transcript variant A (Strn-Mlck), mRNA |
| 0 |
0 |
4 |
3 |
9 |
NM_166128.1 |
CG18255-RG, transcript variant G (Strn-Mlck), mRNA |
| 0 |
0 |
4 |
3 |
9 |
NM_166126.1 |
CG18255-RC, transcript variant C (Strn-Mlck), mRNA |
| 0 |
0 |
4 |
3 |
9 |
NM_166127.1 |
CG18255-RF, transcript variant F (Strn-Mlck), mRNA |
| 0 |
0 |
4 |
3 |
9 |
NM_079030.2 |
CG18255-RB, transcript variant B (Strn-Mlck), mRNA |
| 0 |
0 |
3 |
6 |
5 |
NM_135226.2 |
CG11221-RA (CG11221), mRNA |
| 0 |
0 |
3 |
4 |
8 |
NM_136670.2 |
CG1776-RA (CG1776), mRNA |
| 0 |
0 |
3 |
3 |
2 |
NM_057687.3 |
CG4488-RA (wee), mRNA |
| 0 |
0 |
3 |
2 |
6 |
NM_206620.4 |
CG32782-RD, transcript variant D (tlk), mRNA |
| 0 |
0 |
3 |
2 |
6 |
NM_130717.3 |
CG32782-RC, transcript variant C (tlk), mRNA |
| 0 |
0 |
2 |
7 |
6 |
NM_137515.1 |
CG15072-RA (CG15072), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_079696.4 |
CG10498-RB, transcript variant B (cdc2c), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_169916.1 |
CG10498-RA, transcript variant A (cdc2c), mRNA |
| 0 |
0 |
2 |
1 |
2 |
NM_164806.1 |
CG7795-RB, transcript variant B (CG7795), mRNA |
| 0 |
0 |
2 |
1 |
2 |
NM_135364.1 |
CG7795-RA, transcript variant A (CG7795), mRNA |
| 0 |
0 |
1 |
5 |
4 |
NM_135357.3 |
CG8183-RA, transcript variant A (Khc-73), mRNA |
| 0 |
0 |
1 |
5 |
4 |
NM_176176.1 |
CG8183-RB, transcript variant B (Khc-73), mRNA |
| 0 |
0 |
1 |
4 |
4 |
NM_079464.2 |
CG3354-RA (Mst77F), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_165490.1 |
CG3427-RA (Epac), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_057732.3 |
CG8203-RA (Cdk5), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_141401.1 |
CG2330-RA (CG2330), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_141453.2 |
CG1234-RA (CG1234), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_140583.1 |
CG12272-RA (CG12272), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_057373.2 |
CG2822-RA, transcript variant A (Shaw), mRNA |
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