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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
2556R-4 |
| Symbol |
CG2556 |
Full Name |
CG2556 |
| CG No |
CG2556 |
Old CG No |
CG2556 |
| Synonyms |
CG2556 |
| Accession No (Link to NCBI) |
NM_132577.1 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
male lethal, female semi-lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624
[
PubMed ID = 21666802
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGGAGGAGATCAAGGAGGTAGT |
| Primer Seq. 3' |
aatctagaggtaccGGGTGATCAGCTTCTGCTGTG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 GGAGGAGATC AAGGAGGTAG TGCGGCGCGA GGAGGAGTAC CGCCAGCTGG CCACACTGTC
0061 GTCCAGCGCC TCGATCACTT CCGCTTCGCC GGATTTCGAG ACATACACGG TTAATAGTAA
0121 CTTTGAGCCG AAACTGCAGC AGGATGATGA GCACGATCTG GTCGAAAGTC CAGATCAGGA
0181 CCAGGGCATG CTCGTTACCG CCCAGCCGGC GGCCGTGCAG ACCACCTCGC TCCTGCTGCC
0241 CATCGACGAG CTGTCCAACA CACCATCGCT GGCCTCCAGT GTGAATAGCG CCAAGGAGGA
0301 GAGCCTCGAT GGCCAGCACT CGGATGATTC GGGCATTTCG GCCTCCTCGC AGAGAAACAT
0361 CCTCATCACT GGGATCACCA CGAATGTGGC CAAGCAGCAG CCACTCAAAC TGACCGTGGT
0421 GACGCGTCAG GAGCAGCGTT ACATTGGGCC CAACTGCTAT AATCTGACAC CTGAACCGCC
0481 ACAGCAGAAG CTGATCACCC |
| in silico PCR Fragment |
0001 GGAGGAGATC AAGGAGGTAG TGCGGCGCGA GGAGGAGTAC CGCCAGCTGG CCACACTGTC
0061 GTCCAGCGCC TCGATCACTT CCGCTTCGCC GGATTTCGAG ACATACACGG TTAATAGTAA
0121 CTTTGAGCCG AAACTGCAGC AGGATGATGA GCACGATCTG GTCGAAAGTC CAGATCAGGA
0181 CCAGGGCATG CTCGTTACCG CCCAGCCGGC GGCCGTGCAG ACCACCTCGC TCCTGCTGCC
0241 CATCGACGAG CTGTCCAACA CACCATCGCT GGCCTCCAGT GTGAATAGCG CCAAGGAGGA
0301 GAGCCTCGAT GGCCAGCACT CGGATGATTC GGGCATTTCG GCCTCCTCGC AGAGCAACAT
0361 CCTCATCACT GGGATCACCA CGAATGTGGC CAAGCAGCAG CCACTCAAAC TGACCGTGGT
0421 GACGCGTCAG GAGCAGCGTT ACATTGGGCC CAACTGCTAT AATCTGACAC CTGAACCGCC
0481 ACAGCAGAAG CTGATCACCC |
| Assemble Data |
2556R-4.IR_full 1 GGAGGAGATCAAGGAGGTAGTGCGGCGCGAGGAGGAGTACCGCCAGCTGGCCACACTGTC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2556R-4.in silico 1 GGAGGAGATCAAGGAGGTAGTGCGGCGCGAGGAGGAGTACCGCCAGCTGGCCACACTGTC 60
2556R-4.IR_full 61 GTCCAGCGCCTCGATCACTTCCGCTTCGCCGGATTTCGAGACATACACGGTTAATAGTAA 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2556R-4.in silico 61 GTCCAGCGCCTCGATCACTTCCGCTTCGCCGGATTTCGAGACATACACGGTTAATAGTAA 120
2556R-4.IR_full 121 CTTTGAGCCGAAACTGCAGCAGGATGATGAGCACGATCTGGTCGAAAGTCCAGATCAGGA 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2556R-4.in silico 121 CTTTGAGCCGAAACTGCAGCAGGATGATGAGCACGATCTGGTCGAAAGTCCAGATCAGGA 180
2556R-4.IR_full 181 CCAGGGCATGCTCGTTACCGCCCAGCCGGCGGCCGTGCAGACCACCTCGCTCCTGCTGCC 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2556R-4.in silico 181 CCAGGGCATGCTCGTTACCGCCCAGCCGGCGGCCGTGCAGACCACCTCGCTCCTGCTGCC 240
2556R-4.IR_full 241 CATCGACGAGCTGTCCAACACACCATCGCTGGCCTCCAGTGTGAATAGCGCCAAGGAGGA 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2556R-4.in silico 241 CATCGACGAGCTGTCCAACACACCATCGCTGGCCTCCAGTGTGAATAGCGCCAAGGAGGA 300
2556R-4.IR_full 301 GAGCCTCGATGGCCAGCACTCGGATGATTCGGGCATTTCGGCCTCCTCGCAGAGAAACAT 360
|||||||||||||||||||||||||||||||||||||||||||||||||||||| |||||
2556R-4.in silico 301 GAGCCTCGATGGCCAGCACTCGGATGATTCGGGCATTTCGGCCTCCTCGCAGAGCAACAT 360
2556R-4.IR_full 361 CCTCATCACTGGGATCACCACGAATGTGGCCAAGCAGCAGCCACTCAAACTGACCGTGGT 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2556R-4.in silico 361 CCTCATCACTGGGATCACCACGAATGTGGCCAAGCAGCAGCCACTCAAACTGACCGTGGT 420
2556R-4.IR_full 421 GACGCGTCAGGAGCAGCGTTACATTGGGCCCAACTGCTATAATCTGACACCTGAACCGCC 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2556R-4.in silico 421 GACGCGTCAGGAGCAGCGTTACATTGGGCCCAACTGCTATAATCTGACACCTGAACCGCC 480
2556R-4.IR_full 481 ACAGCAGAAGCTGATCACCC 500
||||||||||||||||||||
2556R-4.in silico 481 ACAGCAGAAGCTGATCACCC 500
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_132577.1 |
CG2556-RA (CG2556), mRNA |
| 0 |
0 |
3 |
3 |
3 |
NM_167157.1 |
CG12117-RA (Sptr), mRNA |
| 0 |
0 |
3 |
2 |
3 |
NM_132722.2 |
CG1434-RA (CG1434), mRNA |
| 0 |
0 |
3 |
2 |
2 |
NM_136037.2 |
CG10275-RA (CG10275), mRNA |
| 0 |
0 |
2 |
8 |
2 |
NM_205901.1 |
CG3304-RC, transcript variant C (CG3304), mRNA |
| 0 |
0 |
2 |
4 |
3 |
NM_079304.2 |
CG11621-RA, transcript variant A (Pi3K68D), mRNA |
| 0 |
0 |
2 |
4 |
3 |
NM_168475.1 |
CG11621-RC, transcript variant C (Pi3K68D), mRNA |
| 0 |
0 |
2 |
4 |
2 |
NM_141130.3 |
CG11489-RB, transcript variant B (CG11489), mRNA |
| 0 |
0 |
2 |
4 |
2 |
NM_176386.1 |
CG11489-RC, transcript variant C (CG11489), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_057245.2 |
CG6189-RA (l(1)1Bi), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_143813.1 |
CG4163-RA (Cyp303a1), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_206017.1 |
CG9339-RD, transcript variant D (CG9339), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_206016.1 |
CG9339-RH, transcript variant H (CG9339), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_165349.2 |
CG9339-RG, transcript variant G (CG9339), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_136229.3 |
CG9339-RA, transcript variant A (CG9339), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_165348.2 |
CG9339-RE, transcript variant E (CG9339), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_165350.2 |
CG9339-RB, transcript variant B (CG9339), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_165351.2 |
CG9339-RF, transcript variant F (CG9339), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_165352.2 |
CG9339-RC, transcript variant C (CG9339), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_134518.2 |
CG11943-RA, transcript variant A (CG11943), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_167687.1 |
CG11943-RB, transcript variant B (CG11943), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_205918.1 |
CG13788-RE, transcript variant E (Gr28b), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_205919.1 |
CG13788-RD, transcript variant D (Gr28b), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_205920.1 |
CG13788-RC, transcript variant C (Gr28b), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_205921.1 |
CG13788-RB, transcript variant B (Gr28b), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_139357.2 |
CG13788-RA, transcript variant A (Gr28b), mRNA |
| 0 |
0 |
1 |
5 |
5 |
NM_205961.1 |
CG33305-RA (CG33305), mRNA |
| 0 |
0 |
1 |
4 |
5 |
NM_165984.1 |
CG17034-RA, transcript variant A (CG17034), mRNA |
| 0 |
0 |
1 |
4 |
5 |
NM_170624.1 |
CG17034-RB, transcript variant B (CG17034), mRNA |
| 0 |
0 |
1 |
4 |
5 |
NM_170625.1 |
CG17034-RC, transcript variant C (CG17034), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
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Figure
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JNK signal
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Caspase-3 signal
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