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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 2341R-1 
 Symbol Ccp84Ad  Full Name Ccp84Ad 
 CG No CG2341  Old CG No CG2341 
 Synonyms unnamed, cc5, CG2341, BG:DS00004.4, Ccp84Ad, Ccp 
 Accession No (Link to NCBI) NM_141423.2 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgCAAGTTCATCGCCCTCTTCGC 
 Primer Seq. 3' 
aatctagaggtaccGGAGCCACGGTCTTGACCACA 
 Predicted Fragment Size 
477 
 IR fragment full Seq 
0001 CAAGTTCATC GCCCTCTTCG CCCTGATCGC CGCCGCCAGC GCGCGGTGTT CTGCCCGTCC 
0061 AGCAGGTGTA CCACGCCGCC CCCGCCGTGG CCACCTACGC CCAAGCACCT GTCGCCGTTG 
0121 CTCACGCCCA GCCGGTTCTT GCCAAGGCCG CCGAGGAGTA CGATCCCCAT CCCCAGTACA 
0181 AGTACGCCTA CGATGTTCAG GACTCCCTCT CCGGAGACTC CAAGAGCCAG GTGGAGGANG 
0241 CGCGATGGCG ACGTGGTCCG CGGAGAGTAC TCCCTGATNC GACGCCGATG GCTACAAGAG 
0301 AACCGTCCAG TACACCGCCG ATCCCATCAA CGGATTCAAC GCCGTTGTCA ACCGTGAGCC 
0361 CCTGGTCAAG GCCGTCGCCG TTGCCCCCGT TGTGAAGACC GTCGCCGCTC CCGTTGCCCA 
0421 CTACGCCGCC CCTGCTGTCG CCCATTACGC NTGCTCCCGC TGTGGTCAAG ACCGTGGCTC 
0481 C 
 in silico PCR Fragment 
0001 CAAGTTCATC GCCCTCTTCG CCCTGATCGC CGCCGCCAGC GC-CGGTGTT CTGCCCGTCC 
0061 AGCAGGTGTA CCACGCCGCC CCCGCCGTGG CCACCTACGC CCAAGCACCT GTCGCCGTTG 
0121 CTCACGCCCA GCCGGTTCTT GCCAAGGCCG CCGAGGAGTA CGATCCCCAT CCCCAGTACA 
0181 AGTACGCCTA CGATGTTCAG GACTCCCTCT CCGGAGACTC CAAGAGCCAG GTGGAGGA-G 
0241 CGCGATGGCG ACGTGGTCCG CGGAGAGTAC TCCCTGAT-C GACGCCGATG GCTACAAGAG 
0301 AACCGTCCAG TACACCGCCG ATCCCATCAA CGGATTCAAC GCCGTTGTCA ACCGTGAGCC 
0361 CCTGGTCAAG GCCGTCGCCG TTGCCCCCGT TGTGAAGACC GTCGCCGCTC CCGTTGCCCA 
0421 CTACGCCGCC CCTGCTGTCG CCCATTACGC -TGCTCCCGC TGTGGTCAAG ACCGTGGCTC 
0481 C 
 Assemble Data 

2341R-1.IR_full       1   CAAGTTCATCGCCCTCTTCGCCCTGATCGCCGCCGCCAGCGCGCGGTGTTCTGCCCGTCC 60
                          |||||||||||||||||||||||||||||||||||||||||| |||||||||||||||||
2341R-1.in silico     1   CAAGTTCATCGCCCTCTTCGCCCTGATCGCCGCCGCCAGCGC-CGGTGTTCTGCCCGTCC 60


2341R-1.IR_full       61  AGCAGGTGTACCACGCCGCCCCCGCCGTGGCCACCTACGCCCAAGCACCTGTCGCCGTTG 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2341R-1.in silico     61  AGCAGGTGTACCACGCCGCCCCCGCCGTGGCCACCTACGCCCAAGCACCTGTCGCCGTTG 120


2341R-1.IR_full       121 CTCACGCCCAGCCGGTTCTTGCCAAGGCCGCCGAGGAGTACGATCCCCATCCCCAGTACA 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2341R-1.in silico     121 CTCACGCCCAGCCGGTTCTTGCCAAGGCCGCCGAGGAGTACGATCCCCATCCCCAGTACA 180


2341R-1.IR_full       181 AGTACGCCTACGATGTTCAGGACTCCCTCTCCGGAGACTCCAAGAGCCAGGTGGAGGANG 240
                          |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| |
2341R-1.in silico     181 AGTACGCCTACGATGTTCAGGACTCCCTCTCCGGAGACTCCAAGAGCCAGGTGGAGGA-G 240


2341R-1.IR_full       241 CGCGATGGCGACGTGGTCCGCGGAGAGTACTCCCTGATNCGACGCCGATGGCTACAAGAG 300
                          |||||||||||||||||||||||||||||||||||||| |||||||||||||||||||||
2341R-1.in silico     241 CGCGATGGCGACGTGGTCCGCGGAGAGTACTCCCTGAT-CGACGCCGATGGCTACAAGAG 300


2341R-1.IR_full       301 AACCGTCCAGTACACCGCCGATCCCATCAACGGATTCAACGCCGTTGTCAACCGTGAGCC 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2341R-1.in silico     301 AACCGTCCAGTACACCGCCGATCCCATCAACGGATTCAACGCCGTTGTCAACCGTGAGCC 360


2341R-1.IR_full       361 CCTGGTCAAGGCCGTCGCCGTTGCCCCCGTTGTGAAGACCGTCGCCGCTCCCGTTGCCCA 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2341R-1.in silico     361 CCTGGTCAAGGCCGTCGCCGTTGCCCCCGTTGTGAAGACCGTCGCCGCTCCCGTTGCCCA 420


2341R-1.IR_full       421 CTACGCCGCCCCTGCTGTCGCCCATTACGCNTGCTCCCGCTGTGGTCAAGACCGTGGCTC 480
                          |||||||||||||||||||||||||||||| |||||||||||||||||||||||||||||
2341R-1.in silico     421 CTACGCCGCCCCTGCTGTCGCCCATTACGC-TGCTCCCGCTGTGGTCAAGACCGTGGCTC 480


2341R-1.IR_full       481 C 481
                          |
2341R-1.in silico     481 C 481



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100.43  461  30  23  47  NM_141423.2  CG2341-RA (Ccp84Ad), mRNA 
29.19   134  84  101  75  NM_141426.1  CG2360-RA (Ccp84Aa), mRNA 
28.32   130  94  87  60  NM_141425.1  CG1252-RA (Ccp84Ab), mRNA 
8.49   39  87  95  72  NM_141421.1  CG1331-RA (Ccp84Af), mRNA 
5.88   27  62  62  68  NM_132038.1  CG4052-RA (CG4052), mRNA 
3.48   16  17  20  29  NM_141420.2  CG2342-RA (Ccp84Ag), mRNA 
2.17   10  39  24  20  NM_141422.2  CG1330-RA (Ccp84Ae), mRNA 
1.08   5  7  5  21  NM_139616.1  CG15007-RA (CG15007), mRNA 
0.65   3  6  47  72  NM_167394.1  CG32603-RA (CG32603), mRNA 
0.21   1  2  2  7  NM_166126.1  CG18255-RC, transcript variant C (Strn-Mlck), mRNA 
0.21   1  2  2  5  NM_166130.1  CG18255-RE, transcript variant E (Strn-Mlck), mRNA 
0   0  12  12  48  NM_141424.2  CG1327-RA (Ccp84Ac), mRNA 
0   0  6  22  46  NM_139618.2  CG1259-RB (CG1259), mRNA 
0   0  5  4  2  NM_134697.1  CG12506-RA (CG12506), mRNA 
0   0  4  6  5  NM_134698.1  CG13947-RA (CG13947), mRNA 
0   0  3  20  12  NM_057558.3  CG16963-RA (Cry), mRNA 
0   0  3  16  30  NM_139411.1  CG1919-RA (CG1919), mRNA 
0   0  3  6  9  NM_142562.1  CG6231-RA, transcript variant A (CG6231), mRNA 
0   0  3  6  9  NM_169864.1  CG6231-RB, transcript variant B (CG6231), mRNA 
0   0  3  6  9  NM_169866.1  CG6231-RD, transcript variant D (CG6231), mRNA 
0   0  3  6  9  NM_169865.1  CG6231-RC, transcript variant C (CG6231), mRNA 
0   0  3  3  5  NM_057926.2  CG10533-RA (Lcp65Af), mRNA 
0   0  3  3  4  NM_079329.2  CG11276-RB, transcript variant B (RpS4), mRNA 
0   0  3  3  4  NM_168537.1  CG11276-RA, transcript variant A (RpS4), mRNA 
0   0  3  3  2  NM_142258.2  CG5516-RA (CG5516), mRNA 
0   0  3  3  2  NM_170046.1  CG31280-RA (Gr94a), mRNA 
0   0  3  2  7  NM_136515.2  CG8709-RA (CG8709), mRNA 
0   0  2  5  13  NM_132711.1  CG1368-RA (CG1368), mRNA 
0   0  2  2  7  NM_142042.2  CG10841-RA (CG10841), mRNA 
0   0  2  2  7  NM_168413.1  CG32062-RB, transcript variant B (CG32062), mRNA 

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