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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 2310R-1 
 Symbol CG2310  Full Name CG2310 
 CG No CG2310  Old CG No CG2310 
 Synonyms CG2310 
 Accession No (Link to NCBI) NM_143437.2 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgAGCCCTGTCCCTACTCTACCC 
 Primer Seq. 3' 
aatctagaggtaccCCTGGATCTCCTTCAGCAGCG 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 AGCCCTGTCC CTACTCTACC CGCTGGCCAT CGTTTCCTCG CTGGTCTGCT GCATCACCTC 
0061 GGCGGTGGCC TGGACGCACT GGCAGTACGT GCTCAATGCC TGCCCGGACA CCAATTGCGG 
0121 CTGCGTGCTG CACGGCAAGA GCACGTTCCA CAGCTTCGAG GGCGGCAACG TCGCCTACTG 
0181 CCACTTCGCC ACCTACGGCC TGCTGCTGCC GCTGCTCTTC GCCGTCGTCC TGGGCGTCTA 
0241 CCACGGCTAC CGCATGTGCA TCGGGCGGGG CAAGCCCAAG GCGNGCACGG CCACCATCCG 
0301 TCAGCGCTCC GGTGACATGA TCGTGGTGAC CACCGAGTCG GAACTCACGC CCGATGGTCT 
0361 CTCGCCCTAC TANCTGGCTG CCCGCCACCG TCATCTCGAC AATAATGGCC GTCTACCAAC 
0421 TGGTCTACTC GGCCGTGTTC ACGGATGGCT TCGAGGTGAC TTGCAAGCAG TACAGGGAGT 
0481 CGCTGCTGAA GGAGATCCAG G 
 in silico PCR Fragment 
0001 AGCCCTGTCC CTACTCTACC CGCTGGCCAT CGTTTCATCG CTGGTCTGCT GCATCACCTC 
0061 GGCGGTGGCC TGGACACACT GGCAGTACGT GCTCAATGCC TGCCCGGACA CCAATTGCGG 
0121 CTGCGTGCTG CACGGCAAGA GCACGTTCCA CAGCTTCGAG GGCGGCAACG TCGCCTACTG 
0181 CCACTTCGCC ACCTACGGCC TGCTGCTGCC GCTGCTCTTC GCCGTCGTCC TGGGCGTCTA 
0241 CCACGGCTAC CGCATGTGCA TCGGTCGGGG CAAGCCCAAG GCGGGCACGG CCACCATCCG 
0301 TCAGCGCTCC GGTGACATGA TCGTGGTGAC CACCGAGTCG GAACTCACGC CCGATGGTCT 
0361 CTCGCCCTAC TA-CTGGCTG CCCGCCACCG TCATCTCGAC AATAATGGCC GTCTACCAAC 
0421 TGGTCTACTC GGCCGTGTTC ACGGATGGCT TCGAGGTGAC TTGCAAGCAG TACAGGGAGT 
0481 CGCTGCTGAA GGAGATCCAG G 
 Assemble Data 

2310R-1.IR_full       1   AGCCCTGTCCCTACTCTACCCGCTGGCCATCGTTTCCTCGCTGGTCTGCTGCATCACCTC 60
                          |||||||||||||||||||||||||||||||||||| |||||||||||||||||||||||
2310R-1.in silico     1   AGCCCTGTCCCTACTCTACCCGCTGGCCATCGTTTCATCGCTGGTCTGCTGCATCACCTC 60


2310R-1.IR_full       61  GGCGGTGGCCTGGACGCACTGGCAGTACGTGCTCAATGCCTGCCCGGACACCAATTGCGG 120
                          ||||||||||||||| ||||||||||||||||||||||||||||||||||||||||||||
2310R-1.in silico     61  GGCGGTGGCCTGGACACACTGGCAGTACGTGCTCAATGCCTGCCCGGACACCAATTGCGG 120


2310R-1.IR_full       121 CTGCGTGCTGCACGGCAAGAGCACGTTCCACAGCTTCGAGGGCGGCAACGTCGCCTACTG 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2310R-1.in silico     121 CTGCGTGCTGCACGGCAAGAGCACGTTCCACAGCTTCGAGGGCGGCAACGTCGCCTACTG 180


2310R-1.IR_full       181 CCACTTCGCCACCTACGGCCTGCTGCTGCCGCTGCTCTTCGCCGTCGTCCTGGGCGTCTA 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2310R-1.in silico     181 CCACTTCGCCACCTACGGCCTGCTGCTGCCGCTGCTCTTCGCCGTCGTCCTGGGCGTCTA 240


2310R-1.IR_full       241 CCACGGCTACCGCATGTGCATCGGGCGGGGCAAGCCCAAGGCGNGCACGGCCACCATCCG 300
                          |||||||||||||||||||||||| |||||||||||||||||| ||||||||||||||||
2310R-1.in silico     241 CCACGGCTACCGCATGTGCATCGGTCGGGGCAAGCCCAAGGCGGGCACGGCCACCATCCG 300


2310R-1.IR_full       301 TCAGCGCTCCGGTGACATGATCGTGGTGACCACCGAGTCGGAACTCACGCCCGATGGTCT 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2310R-1.in silico     301 TCAGCGCTCCGGTGACATGATCGTGGTGACCACCGAGTCGGAACTCACGCCCGATGGTCT 360


2310R-1.IR_full       361 CTCGCCCTACTANCTGGCTGCCCGCCACCGTCATCTCGACAATAATGGCCGTCTACCAAC 420
                          |||||||||||| |||||||||||||||||||||||||||||||||||||||||||||||
2310R-1.in silico     361 CTCGCCCTACTA-CTGGCTGCCCGCCACCGTCATCTCGACAATAATGGCCGTCTACCAAC 420


2310R-1.IR_full       421 TGGTCTACTCGGCCGTGTTCACGGATGGCTTCGAGGTGACTTGCAAGCAGTACAGGGAGT 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2310R-1.in silico     421 TGGTCTACTCGGCCGTGTTCACGGATGGCTTCGAGGTGACTTGCAAGCAGTACAGGGAGT 480


2310R-1.IR_full       481 CGCTGCTGAAGGAGATCCAGG 501
                          |||||||||||||||||||||
2310R-1.in silico     481 CGCTGCTGAAGGAGATCCAGG 501



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_143437.2  CG2310-RA, transcript variant A (CG2310), mRNA 
100   482  0  0  0  NM_170411.1  CG2310-RB, transcript variant B (CG2310), mRNA 
0.41   2  2  2  5  NM_139514.2  CG11537-RC, transcript variant C (CG11537), mRNA 
0.41   2  2  2  5  NM_167989.1  CG11537-RB, transcript variant B (CG11537), mRNA 
0.41   2  2  2  5  NM_167988.1  CG11537-RA, transcript variant A (CG11537), mRNA 
0   0  5  2  5  NM_080161.1  CG3496-RA (vir), mRNA 
0   0  4  6  5  NM_164887.1  CG31714-RA (CG31714), mRNA 
0   0  3  7  12  NM_143018.2  CG5794-RD, transcript variant D (CG5794), mRNA 
0   0  3  7  12  NM_170158.1  CG5794-RB, transcript variant B (CG5794), mRNA 
0   0  3  7  10  NM_206564.1  CG5794-RE, transcript variant E (CG5794), mRNA 
0   0  3  6  28  NM_167000.1  CG32778-RA (CG32778), mRNA 
0   0  3  4  9  NM_141702.2  CG12806-RA (Teh1), mRNA 
0   0  3  3  7  NM_133114.2  CG32541-RA (CG32541), mRNA 
0   0  3  3  2  NM_079274.2  CG4167-RA (Hsp67Ba), mRNA 
0   0  3  3  2  NM_140060.1  CG3445-RA (phol), mRNA 
0   0  3  2  2  NM_140033.1  CG4684-RA (nwk), mRNA 
0   0  3  2  2  NM_141121.2  CG7139-RA, transcript variant A (CG7139), mRNA 
0   0  3  2  2  NM_001014717.1  CG33548-RA, transcript variant A (msta), mRNA 
0   0  3  2  2  NM_168947.1  CG7139-RB, transcript variant B (CG7139), mRNA 
0   0  2  20  60  NM_001038734.1  CG16902-RC (Hr4), mRNA 
0   0  2  13  51  NM_169782.1  CG31243-RA, transcript variant A (cpo), mRNA 
0   0  2  13  51  NM_169781.1  CG31243-RE, transcript variant E (cpo), mRNA 
0   0  2  13  51  NM_169783.1  CG31243-RB, transcript variant B (cpo), mRNA 
0   0  2  12  32  NM_080105.2  CG31243-RF, transcript variant F (cpo), mRNA 
0   0  2  11  27  NM_001014631.1  CG31243-RH, transcript variant H (cpo), mRNA 
0   0  2  11  24  NM_001014632.1  CG31243-RG, transcript variant G (cpo), mRNA 
0   0  2  9  29  NM_166872.1  CG3638-RD, transcript variant D (CG3638), mRNA 
0   0  2  9  29  NM_130541.3  CG3638-RC, transcript variant C (CG3638), mRNA 
0   0  2  9  29  NM_166873.1  CG3638-RB, transcript variant B (CG3638), mRNA 
0   0  2  9  29  NM_166874.1  CG3638-RA, transcript variant A (CG3638), mRNA 
 Wing Disc
GFP
 Puc
 Caspase
DAPI
 Merge
 Figure
JNK signal
N
 
DB
 
DC
+- 
DM
 
VM
 
VB
 
Caspase-3 signal
N
+ 
DB
+ 
DC
+ 
DM
+ 
VM
 
VB
 
Dcompartment   Penetrance
     

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