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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
2263R-1 |
| Symbol |
CG2263 |
Full Name |
CG2263 |
| CG No |
CG2263 |
Old CG No |
CG2263 |
| Synonyms |
CG2263 |
| Accession No (Link to NCBI) |
NM_132220.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Ohhara Y, Nakamura A, Kato Y, Yamakawa-Kobayashi K.
Chaperonin TRiC/CCT supports mitotic exit and entry into endocycle in Drosophila.
PLoS Genet (2019) 15(4) e1008121
[
PubMed ID = 31034473
]
[
RRC reference
]
Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624
[
PubMed ID = 21666802
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgATGCATCCCGATCTCACGGAG |
| Primer Seq. 3' |
aatctagaggtaccGTTTCCTGGAGGAGCTTGCGT |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 ATGCATCCCG ATCTCACGGA GCGCATTCTC CAGCATTTGG AGACCACCGA CAAGGCGGAC
0061 ACACTGGACC TGGCCGCACT CTTTGCCGAG GATCACCAGA AGATTGTGGG CTCATTGAAG
0121 AGCATACAGG CGCATGGCGA ACNTAGTCAC CGCGGAAACG GTCACCCACA AGAGTTTGGG
0181 CCTCACAGAC GAGGGTCGCG CCGTCGTGGA GAATGGCAGT CATGAGGCAC TGGTCTACGA
0241 CCTAGTTCCG CCGGAGGGAA TCGCCCAGGC GGCTCTAATG GCAGNCGGTG GCGCAAATGC
0301 CAAAGTGGGC TTTAGNAAAG CCATGTCGCA CGGCTGGATT TTGGTGGACA ANGAGCGTNC
0361 ACNCCGCCGC TGGTGCGCCG CAAGGTGNGA CACCATTACG GATGTGGTGC GCAATCAACT
0421 GCAGCAGGTG GCGCTGGGAA AGGGTGACCA GCTGCCTGCC AAGGAGGTGG CCGACTTCAA
0481 GAANACGCAA GCTCCTCCAG GAAAC |
| in silico PCR Fragment |
0001 ATGCATCCCG ATCTCACGGA GCGCATTCTC CAGCATTTGG AGACCACCGA CAAGGCGGAC
0061 ACACTGGACC TGGCCGCACT CTTTGCCGAG GATCACCAGA AGATTGTGGG CTCATTGAAG
0121 AGCATACAGG CGCATGGCGA AC-TAGTCAC CGCGGAAACG GTCACCCACA AGAGTTTGGG
0181 CCTCACAGAC GAGGGTCGCG CCGTCGTGGA GAATGGCAGT CATGAGGCAC TGGTCTACGA
0241 CCTAGTTCCG CCGGAGGGAA TCGCCCAGGC GGCTCTAATG GCAGCCGGTG GCGCAAATGC
0301 CAAAGTGGGC TTTAGCAAAG CCATGTCGCA CGGCTGGATT TTGGTGGACA A-GAGCGT-C
0361 ACGCCGCCGC TGGTGCGCCG CAAGGTG-GA CACCATTACG GATGTGGTGC GCAATCAACT
0421 GCAGCAGGTG GCGCTGGGAA AGGGTGACCA GCTGCCTGCC AAGGAGGTGG CCGACTTCAA
0481 GAA-ACGCAA GCTCCTCCAG GAAAC |
| Assemble Data |
2263R-1.IR_full 1 ATGCATCCCGATCTCACGGAGCGCATTCTCCAGCATTTGGAGACCACCGACAAGGCGGAC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2263R-1.in silico 1 ATGCATCCCGATCTCACGGAGCGCATTCTCCAGCATTTGGAGACCACCGACAAGGCGGAC 60
2263R-1.IR_full 61 ACACTGGACCTGGCCGCACTCTTTGCCGAGGATCACCAGAAGATTGTGGGCTCATTGAAG 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2263R-1.in silico 61 ACACTGGACCTGGCCGCACTCTTTGCCGAGGATCACCAGAAGATTGTGGGCTCATTGAAG 120
2263R-1.IR_full 121 AGCATACAGGCGCATGGCGAACNTAGTCACCGCGGAAACGGTCACCCACAAGAGTTTGGG 180
|||||||||||||||||||||| |||||||||||||||||||||||||||||||||||||
2263R-1.in silico 121 AGCATACAGGCGCATGGCGAAC-TAGTCACCGCGGAAACGGTCACCCACAAGAGTTTGGG 180
2263R-1.IR_full 181 CCTCACAGACGAGGGTCGCGCCGTCGTGGAGAATGGCAGTCATGAGGCACTGGTCTACGA 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2263R-1.in silico 181 CCTCACAGACGAGGGTCGCGCCGTCGTGGAGAATGGCAGTCATGAGGCACTGGTCTACGA 240
2263R-1.IR_full 241 CCTAGTTCCGCCGGAGGGAATCGCCCAGGCGGCTCTAATGGCAGNCGGTGGCGCAAATGC 300
|||||||||||||||||||||||||||||||||||||||||||| |||||||||||||||
2263R-1.in silico 241 CCTAGTTCCGCCGGAGGGAATCGCCCAGGCGGCTCTAATGGCAGCCGGTGGCGCAAATGC 300
2263R-1.IR_full 301 CAAAGTGGGCTTTAGNAAAGCCATGTCGCACGGCTGGATTTTGGTGGACAANGAGCGTNC 360
||||||||||||||| ||||||||||||||||||||||||||||||||||| |||||| |
2263R-1.in silico 301 CAAAGTGGGCTTTAGCAAAGCCATGTCGCACGGCTGGATTTTGGTGGACAA-GAGCGT-C 360
2263R-1.IR_full 361 ACNCCGCCGCTGGTGCGCCGCAAGGTGNGACACCATTACGGATGTGGTGCGCAATCAACT 420
|| |||||||||||||||||||||||| ||||||||||||||||||||||||||||||||
2263R-1.in silico 361 ACGCCGCCGCTGGTGCGCCGCAAGGTG-GACACCATTACGGATGTGGTGCGCAATCAACT 420
2263R-1.IR_full 421 GCAGCAGGTGGCGCTGGGAAAGGGTGACCAGCTGCCTGCCAAGGAGGTGGCCGACTTCAA 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2263R-1.in silico 421 GCAGCAGGTGGCGCTGGGAAAGGGTGACCAGCTGCCTGCCAAGGAGGTGGCCGACTTCAA 480
2263R-1.IR_full 481 GAANACGCAAGCTCCTCCAGGAAAC 505
||| |||||||||||||||||||||
2263R-1.in silico 481 GAA-ACGCAAGCTCCTCCAGGAAAC 505
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_132220.2 |
CG2263-RA (CG2263), mRNA |
| 0 |
0 |
5 |
3 |
2 |
NM_135959.1 |
CG17996-RA (CG17996), mRNA |
| 0 |
0 |
2 |
5 |
4 |
NM_143012.1 |
CG5789-RA (CG5789), mRNA |
| 0 |
0 |
2 |
4 |
2 |
NM_164500.1 |
CG2862-RB, transcript variant B (CG2862), mRNA |
| 0 |
0 |
2 |
4 |
2 |
NM_134867.2 |
CG2862-RA, transcript variant A (CG2862), mRNA |
| 0 |
0 |
2 |
3 |
6 |
NM_165630.1 |
CG8266-RB, transcript variant B (sec31), mRNA |
| 0 |
0 |
2 |
3 |
6 |
NM_136570.2 |
CG8266-RA, transcript variant A (sec31), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_168802.1 |
CG32210-RA (CG32210), mRNA |
| 0 |
0 |
2 |
2 |
6 |
NM_138041.2 |
CG4049-RA (CG4049), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_079245.2 |
CG7436-RA (Nmt), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_136477.2 |
CG30497-RA, transcript variant A (CG30497), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_165555.1 |
CG30497-RB, transcript variant B (CG30497), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_165556.1 |
CG30497-RC, transcript variant C (CG30497), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_137238.2 |
CG8403-RA (SP2353), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_057785.3 |
CG5373-RA (Pi3K59F), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_001014711.1 |
CG3114-RC, transcript variant C (ewg), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_001014709.1 |
CG3114-RE, transcript variant E (ewg), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_001014710.1 |
CG3114-RD, transcript variant D (ewg), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_166836.1 |
CG3114-RA, transcript variant A (ewg), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_057544.3 |
CG3114-RB, transcript variant B (ewg), mRNA |
| 0 |
0 |
1 |
5 |
3 |
NM_078605.1 |
CG6211-RA (gce), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_078525.2 |
CG10961-RA (Traf2), mRNA |
| 0 |
0 |
1 |
3 |
8 |
NM_136986.2 |
CG3884-RB, transcript variant B (CG3884), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_057450.3 |
CG9704-RB, transcript variant B (Nrt), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_079996.2 |
CG18024-RA (SoxN), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_168682.1 |
CG9704-RA, transcript variant A (Nrt), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_130559.2 |
CG14788-RA (l(1)G0431), mRNA |
| 0 |
0 |
1 |
2 |
7 |
NM_137100.2 |
CG8503-RA (CG8503), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_134796.2 |
CG4238-RA (CG4238), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_001043070.1 |
CG34146-RA (brp), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
JNK signal
| N |
 |
| +- |
|
| DB |
 |
| + |
|
| DC |
 |
| + |
|
| DM |
 |
| ++ |
|
| VM |
 |
| + |
|
| VB |
 |
| |
|
|
Caspase-3 signal
| N |
|
| + |
|
| DB |
|
| + |
|
| DC |
|
| |
|
| DM |
|
| ++ |
|
| VM |
|
| + |
|
| VB |
|
| |
|
|
| Dcompartment |
|
Penetrance |
| ++ |
|
|
|
|
|
|
