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NIG-Fly - Fly Stocks of National Institute of Genetics (NIG) Drosophila strain -NIG-Fly - Fly Stocks of National Institute of Genetics (NIG) Drosophila strain -NIG-Fly - Fly Stocks of National Institute of Genetics (NIG) Drosophila strain -
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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 2256R-2 
 Symbol CG2256  Full Name CG2256 
 CG No CG2256  Old CG No CG2256 
 Synonyms CG2256 
 Accession No (Link to NCBI) NM_132209.1 
 Inserted Chr. X 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624 [ PubMed ID = 21666802 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgCGCGGTTTACGAAGGACGAGC 
 Primer Seq. 3' 
aatctagaggtaccGACCTTGCCGTCCTTGTCCAG 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 CGCGGTTTAC GAAGGACGAG CTGGATGCGT TGTGCCGCAT CTATCGCAAG CTGGTATCAA 
0061 ATTGTCAATA TGCGGCCAAG ACGCTGGCCT CCAGCTCCTC AAGCGCAGCT ATTGCCAGGC 
0121 CACATGCCGC CGTGGAGGGC ATCGATCGCA TTGTGTTTCG CGAACTGTTG CACAGCACTT 
0181 TCGACATCGT CACCGAGGAG ATCCTGATGG AGCGCATCTT CTGCAGCTGG GACAAGGCCC 
0241 ACGAGGGTTT GCCCCTNTCG CCTCGAGGGC TGGCTCATTG GACTTTCAAC ATTNCCTGCG 
0301 CGGTACGCCG GCGGAACGGG CGGCATTTTG CTTCCGGGTT TACGATCTGA ACACGGATGG 
0361 CTTCATCACC AAGGATGAAA TGTTTACGCT GCTGCGAAAT TGCCTGATAA AACAACCGCA 
0421 GGACGAAGAT CCCGACGAGG GCGTCAAGGA TCTGGTGGAA ATTGTGCTCA AGAAGTTCGA 
0481 CCTGGACAAG GACGGCAAGG TC 
 in silico PCR Fragment 
0001 CGCGGTTTAC GAAGGACGAG CTGGATGCGT TGTGCCGCAT CTATCGCAAG CTGGTATCAA 
0061 ATTGTCAATA TGCGGCCAAG ACGCTGGCCT CCAGCTCCTC GAGCGCAGCT ATTGCCAAGC 
0121 CACATGCTGC CGTGGAGGGC ATCGATCGCA TTGTGTTCCG CGAGCTGTTG CACAGCACTT 
0181 TCGACATCGT CACCGAGGAG ATCCTGATGG AGCGCATCTT CTGCAGCTGG GACAAGGCCC 
0241 ACGAGGGTTT GCCCCT-TCG CCTCGAGGGC TGGCTCATTG GACTTTCAAC ATT-CCTGCG 
0301 CGGCACGCCG GCGGAACGGG CGGCTTTTTG CTTCCGGGTT TACGACCTGA ACACGGATGG 
0361 CTTCATCACC AAGGATGAAA TGTTTACGCT GCTGCGAAAT TGCCTGATAA AACAACCGCA 
0421 GGACGAAGAT CCCGACGAGG GCGTCAAGGA TCTGGTGGAG ATTGTGCTCA AGAAGTTCGA 
0481 CCTGGACAAG GACGGCAAGG TC 
 Assemble Data 

2256R-2.IR_full       1   CGCGGTTTACGAAGGACGAGCTGGATGCGTTGTGCCGCATCTATCGCAAGCTGGTATCAA 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2256R-2.in silico     1   CGCGGTTTACGAAGGACGAGCTGGATGCGTTGTGCCGCATCTATCGCAAGCTGGTATCAA 60


2256R-2.IR_full       61  ATTGTCAATATGCGGCCAAGACGCTGGCCTCCAGCTCCTCAAGCGCAGCTATTGCCAGGC 120
                          |||||||||||||||||||||||||||||||||||||||| |||||||||||||||| ||
2256R-2.in silico     61  ATTGTCAATATGCGGCCAAGACGCTGGCCTCCAGCTCCTCGAGCGCAGCTATTGCCAAGC 120


2256R-2.IR_full       121 CACATGCCGCCGTGGAGGGCATCGATCGCATTGTGTTTCGCGAACTGTTGCACAGCACTT 180
                          ||||||| ||||||||||||||||||||||||||||| ||||| ||||||||||||||||
2256R-2.in silico     121 CACATGCTGCCGTGGAGGGCATCGATCGCATTGTGTTCCGCGAGCTGTTGCACAGCACTT 180


2256R-2.IR_full       181 TCGACATCGTCACCGAGGAGATCCTGATGGAGCGCATCTTCTGCAGCTGGGACAAGGCCC 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2256R-2.in silico     181 TCGACATCGTCACCGAGGAGATCCTGATGGAGCGCATCTTCTGCAGCTGGGACAAGGCCC 240


2256R-2.IR_full       241 ACGAGGGTTTGCCCCTNTCGCCTCGAGGGCTGGCTCATTGGACTTTCAACATTNCCTGCG 300
                          |||||||||||||||| |||||||||||||||||||||||||||||||||||| ||||||
2256R-2.in silico     241 ACGAGGGTTTGCCCCT-TCGCCTCGAGGGCTGGCTCATTGGACTTTCAACATT-CCTGCG 300


2256R-2.IR_full       301 CGGTACGCCGGCGGAACGGGCGGCATTTTGCTTCCGGGTTTACGATCTGAACACGGATGG 360
                          ||| |||||||||||||||||||| |||||||||||||||||||| ||||||||||||||
2256R-2.in silico     301 CGGCACGCCGGCGGAACGGGCGGCTTTTTGCTTCCGGGTTTACGACCTGAACACGGATGG 360


2256R-2.IR_full       361 CTTCATCACCAAGGATGAAATGTTTACGCTGCTGCGAAATTGCCTGATAAAACAACCGCA 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2256R-2.in silico     361 CTTCATCACCAAGGATGAAATGTTTACGCTGCTGCGAAATTGCCTGATAAAACAACCGCA 420


2256R-2.IR_full       421 GGACGAAGATCCCGACGAGGGCGTCAAGGATCTGGTGGAAATTGTGCTCAAGAAGTTCGA 480
                          ||||||||||||||||||||||||||||||||||||||| ||||||||||||||||||||
2256R-2.in silico     421 GGACGAAGATCCCGACGAGGGCGTCAAGGATCTGGTGGAGATTGTGCTCAAGAAGTTCGA 480


2256R-2.IR_full       481 CCTGGACAAGGACGGCAAGGTC 502
                          ||||||||||||||||||||||
2256R-2.in silico     481 CCTGGACAAGGACGGCAAGGTC 502



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  2  NM_132209.1  CG2256-RB (CG2256), mRNA 
0.2   1  3  3  3  NM_079290.2  CG6721-RA, transcript variant A (Gap1), mRNA 
0.2   1  3  3  3  NM_168382.1  CG6721-RB, transcript variant B (Gap1), mRNA 
0   0  4  2  5  NM_205892.1  CG3539-RC, transcript variant C (Slh), mRNA 
0   0  4  2  5  NM_205891.1  CG3539-RD, transcript variant D (Slh), mRNA 
0   0  3  4  3  NM_170009.2  CG31170-RB, transcript variant B (epsin-like), mRNA 
0   0  3  4  3  NM_170010.1  CG31170-RA, transcript variant A (epsin-like), mRNA 
0   0  3  4  2  NM_057454.3  CG16720-RA, transcript variant A (5-HT1A), mRNA 
0   0  3  4  2  NM_166322.1  CG16720-RB, transcript variant B (5-HT1A), mRNA 
0   0  3  2  10  NM_138121.2  CG16932-RA, transcript variant A (Eps-15), mRNA 
0   0  3  2  10  NM_166686.1  CG16932-RC, transcript variant C (Eps-15), mRNA 
0   0  3  2  10  NM_166687.1  CG16932-RB, transcript variant B (Eps-15), mRNA 
0   0  2  6  12  NM_167865.1  CG32333-RA, transcript variant A (CG32333), mRNA 
0   0  2  5  8  NM_164436.1  CG17646-RB, transcript variant B (CG17646), mRNA 
0   0  2  5  8  NM_134774.2  CG17646-RA, transcript variant A (CG17646), mRNA 
0   0  2  4  7  NM_165794.2  CG11895-RA (stan), mRNA 
0   0  2  4  3  NM_166022.1  CG30481-RA, transcript variant A (mRpL53), mRNA 
0   0  2  4  3  NM_001032246.1  CG33155-RD, transcript variant D (CG33155), mRNA 
0   0  2  4  3  NM_144091.1  CG15411-RA (CG15411), mRNA 
0   0  2  4  3  NM_001032248.1  CG33155-RA, transcript variant A (CG33155), mRNA 
0   0  2  4  3  NM_001032247.1  CG33155-RC, transcript variant C (CG33155), mRNA 
0   0  2  3  4  NM_143774.2  CG8392-RA (l(2)05070), mRNA 
0   0  2  2  2  NM_141169.2  CG10712-RA, transcript variant A (Chro), mRNA 
0   0  2  2  2  NM_168976.1  CG10712-RC, transcript variant C (Chro), mRNA 
0   0  2  2  2  NM_168975.1  CG10712-RB, transcript variant B (Chro), mRNA 
0   0  1  6  7  NM_078700.2  CG1849-RA (run), mRNA 
0   0  1  5  7  NM_170358.1  CG5508-RC, transcript variant C (CG5508), mRNA 
0   0  1  5  7  NM_170359.1  CG5508-RB, transcript variant B (CG5508), mRNA 
0   0  1  5  7  NM_143340.2  CG5508-RA, transcript variant A (CG5508), mRNA 
0   0  1  5  4  NM_137249.1  CG18243-RA (Ptp52F), mRNA 

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