NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 2229R-3

Symbol Jon99Fii Full Name Jonah 99Fii

CG No CG2229 Old CG No CG2229

Synonyms CG2229, Jon99F, SP135, Jon99Fii

Accession No (Link to NCBI) NM_143542.1

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
[Please submit your publication] Kambris Z, Brun S, Jang IH, Nam HJ, Romeo Y, Takahashi K, Lee WJ, Ueda R, Lemaitre B.
Drosophila immunity: a large-scale in vivo RNAi screen identifies five serine proteases required for Toll activation.
Curr Biol (2006) 16(8) 808-13 [ PubMed ID = 16631589 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCCTACATCGTGGGTCTCCTTT

Primer Seq. 3'

aatctagaggtaccTCACCTCCGCAGGTGGACTTT

Predicted Fragment Size

IR fragment full Seq

0001 CCTACATCGT GGGTCTCCTT TTCAGCGGCA ATGGAAACTG GTGGTGCGGT GGCTCGATCA 
0061 TCGGCAACAC CTGGGTCCTG ACCGCCGCCC ACTGCACCAA CGGAGCCAGT GGAGTGACCA 
0121 TCAACTACGG AGCCAGTCTG CGCAACCAGC CCCAGTACAC CCACTGGGTT GGAAGCGGAA 
0181 ACTTCGTCCA GCACCACCAC TACAACAGCG GCAACCTGCA CAACGACATC TCCCTGATCC 
0241 GCACCCCGCA CGTCGACTTC TGGCACCTGG TCAACAAGGT GGAGTTGCCC AGCTACAACG 
0301 ACCGCTACCA GGACTACGCC GGATGGTGGG CCGTGGcCTC CGGATGGGGC GGCACCTACG 
0361 ATGGCAGCCC ACTGCCCGAC TGGCTCCAGG CTGTCGATGT CCAGATCATG TCCCAGAGTG 
0421 ATTGCAGCCG CACTTGGTCT CTCCACGACA ACATGATTTG CATCAACACC AACGGCGGAA 
0481 AGTCCAcCNG CGGAGGTGA

in silico PCR Fragment

0001 CCTACATCGT GGGTCTCCTT TTCAGTGGCA ATGGAAACTG GTGGTGCGGT GGCTCGATCA 
0061 TCGGCAACAC CTGGGTCCTG ACCGCCGCCC ACTGCACCAA CGGAGCCAGT GGAGTGACCA 
0121 TCAACTACGG AGCCAGTCTG CGCAACCAGC CCCAGTACAC CCACTGGGTT GGAAGCGGAA 
0181 ACTTCGTCCA GCACCACCAC TACAACAGCG GCAACCTGCA CAACGACATC TCCCTGATCC 
0241 GCACCCCGCA CGTCGACTTC TGGCACCTGG TCAACAAGGT GGAGTTGCCC AGCTACAACG 
0301 ACCGCTACCA GGACTACGCC GGATGGTGGG CCGTGGCCTC CGGATGGGGC GGCACCTACG 
0361 ATGGCAGCCC ACTGCCCGAC TGGCTCCAGG CTGTCGATGT CCAGATCATG TCCCAGAGTG 
0421 ATTGCAGCCG CTCGTGGTCT CTCCACGACA ACATGATTTG CATCAACACC AACGGCGGAA 
0481 AGTCCACCTG CGGAGGTGA

Assemble Data


2229R-3.IR_full       1   CCTACATCGTGGGTCTCCTTTTCAGCGGCAATGGAAACTGGTGGTGCGGTGGCTCGATCA 60
                          ||||||||||||||||||||||||| ||||||||||||||||||||||||||||||||||
2229R-3.in silico     1   CCTACATCGTGGGTCTCCTTTTCAGTGGCAATGGAAACTGGTGGTGCGGTGGCTCGATCA 60


2229R-3.IR_full       61  TCGGCAACACCTGGGTCCTGACCGCCGCCCACTGCACCAACGGAGCCAGTGGAGTGACCA 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2229R-3.in silico     61  TCGGCAACACCTGGGTCCTGACCGCCGCCCACTGCACCAACGGAGCCAGTGGAGTGACCA 120


2229R-3.IR_full       121 TCAACTACGGAGCCAGTCTGCGCAACCAGCCCCAGTACACCCACTGGGTTGGAAGCGGAA 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2229R-3.in silico     121 TCAACTACGGAGCCAGTCTGCGCAACCAGCCCCAGTACACCCACTGGGTTGGAAGCGGAA 180


2229R-3.IR_full       181 ACTTCGTCCAGCACCACCACTACAACAGCGGCAACCTGCACAACGACATCTCCCTGATCC 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2229R-3.in silico     181 ACTTCGTCCAGCACCACCACTACAACAGCGGCAACCTGCACAACGACATCTCCCTGATCC 240


2229R-3.IR_full       241 GCACCCCGCACGTCGACTTCTGGCACCTGGTCAACAAGGTGGAGTTGCCCAGCTACAACG 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2229R-3.in silico     241 GCACCCCGCACGTCGACTTCTGGCACCTGGTCAACAAGGTGGAGTTGCCCAGCTACAACG 300


2229R-3.IR_full       301 ACCGCTACCAGGACTACGCCGGATGGTGGGCCGTGGCCTCCGGATGGGGCGGCACCTACG 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2229R-3.in silico     301 ACCGCTACCAGGACTACGCCGGATGGTGGGCCGTGGCCTCCGGATGGGGCGGCACCTACG 360


2229R-3.IR_full       361 ATGGCAGCCCACTGCCCGACTGGCTCCAGGCTGTCGATGTCCAGATCATGTCCCAGAGTG 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2229R-3.in silico     361 ATGGCAGCCCACTGCCCGACTGGCTCCAGGCTGTCGATGTCCAGATCATGTCCCAGAGTG 420


2229R-3.IR_full       421 ATTGCAGCCGCACTTGGTCTCTCCACGACAACATGATTTGCATCAACACCAACGGCGGAA 480
                          ||||||||||| | ||||||||||||||||||||||||||||||||||||||||||||||
2229R-3.in silico     421 ATTGCAGCCGCTCGTGGTCTCTCCACGACAACATGATTTGCATCAACACCAACGGCGGAA 480


2229R-3.IR_full       481 AGTCCACCNGCGGAGGTGA 499
                          |||||||| ||||||||||
2229R-3.in silico     481 AGTCCACCTGCGGAGGTGA 499

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	481	0	0	0	NM_143542.1	CG2229-RA (Jon99Fii), mRNA
78.37	377	68	26	10	NM_143543.2	CG18030-RA (Jon99Fi), mRNA
46.36	223	73	110	30	NM_079830.2	CG31034-RA (Jon99Cii), mRNA
42.41	204	91	110	31	NM_170451.2	CG31362-RA (Jon99Ciii), mRNA
3.74	18	29	53	79	NM_165618.1	CG8579-RA (Jon44E), mRNA
1.87	9	35	38	59	NM_135038.1	CG8869-RA (Jon25Bii), mRNA
1.03	5	41	64	51	NM_135037.1	CG8871-RA (Jon25Biii), mRNA
0.83	4	42	50	64	NM_168271.1	CG7118-RA (Jon66Ci), mRNA
0.83	4	2	3	4	NM_138066.2	CG15873-RA (CG15873), mRNA
0.62	3	4	6	6	NM_143287.1	CG5909-RA (CG5909), mRNA
0.41	2	11	18	19	NM_079220.1	CG6457-RA (yip7), mRNA
0.2	1	34	58	56	NM_139960.1	CG7170-RA (Jon66Cii), mRNA
0.2	1	20	15	25	NM_139755.2	CG6467-RA (Jon65Aiv), mRNA
0.2	1	11	82	45	NM_001038784.1	CG8867-RB, transcript variant B (Jon25Bi), mRNA
0.2	1	11	82	45	NM_078753.2	CG8867-RA, transcript variant A (Jon25Bi), mRNA
0.2	1	5	10	27	NM_139753.1	CG10477-RA (CG10477), mRNA
0.2	1	3	2	13	NM_141239.1	CG17735-RA (CG17735), mRNA
0.2	1	2	2	3	NM_132921.1	CG9673-RA (CG9673), mRNA
0	0	19	26	49	NM_139757.1	CG6580-RA (Jon65Aii), mRNA
0	0	12	31	49	NM_139758.1	CG10475-RA (Jon65Ai), mRNA
0	0	7	2	2	NM_166886.1	CG32808-RA (CG32808), mRNA
0	0	4	6	6	NM_136265.2	CG17571-RA (CG17571), mRNA
0	0	4	4	11	NM_140737.1	CG6298-RA (Jon74E), mRNA
0	0	4	2	2	NM_205906.1	CG10033-RH, transcript variant H (for), mRNA
0	0	4	2	2	NM_205904.1	CG10033-RI, transcript variant I (for), mRNA
0	0	4	2	2	NM_058139.3	CG10033-RA, transcript variant A (for), mRNA
0	0	3	11	29	NM_140084.1	CG18180-RA (CG18180), mRNA
0	0	3	2	2	NM_079268.2	CG10923-RA (Klp67A), mRNA
0	0	2	24	35	NM_139756.2	CG6483-RA (Jon65Aiii), mRNA
0	0	2	10	2	NM_137222.1	CG8299-RA (CG8299), mRNA

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