National Institute of Genetics
Contact us      Quick Search       
NIG-Fly - Fly Stocks of National Institute of Genetics (NIG) Drosophila strain -NIG-Fly - Fly Stocks of National Institute of Genetics (NIG) Drosophila strain -NIG-Fly - Fly Stocks of National Institute of Genetics (NIG) Drosophila strain -
Home Home
Japanese | English  
Notice:

Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
NIG-RNAi Strains Detail
Request : Click the "Order" button to request for this stock discarded request_help.gif
Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 2200R-2 
 Symbol CG2200  Full Name CG2200 
 CG No CG2200  Old CG No CG2200 
 Synonyms CG2200 
 Accession No (Link to NCBI) NM_132624.1 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624 [ PubMed ID = 21666802 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgGGCCAGCTGGAGGATCTCTTC 
 Primer Seq. 3' 
aatctagaggtaccACGGGCAGTCCATGGTATGCG 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 GGCCAGCTGG AGGATCTCTT CAAGAGTGCC AATGTGAAGA CCGTGCTCTT TGTGCCCTAC 
0061 GCTCTGCGGG ATCACGACAA GTACACTGCC ACCGTGCGCG ATGCACTGCA GCCGTGGGGA 
0121 TTCAATGTGG AGGGACTGCA CACGAAGCCG GATCGCGAGC AGGCATTGCG GGAGGCACAG 
0181 GCCATCTTTG TGGGCGGCGG CAACACCTTT GTGCTGCTGC GTTCGCTCTA CGAGATGAAG 
0241 TTGCTCGATC CGATTAGGGA ACTGGTGCTC CAGCGTGGAT TGCCTTACGT GGGCAGCAGT 
0301 GCGGGCACAA ATGTGGCAAC TCGATCCATT CACACCACCA ACGACATGCC GGTGGCCTAT 
0361 CCGCCGAGCT TTGAGGCACT CGCCCTCGTT CCCTTCAACA TCAATCCGCA CTATCTGGAT 
0421 CCGGAGGCGG GTAGTCGGCA CAAGGGCGAG ACGCGAGACG AGCGGCTGGA GGAGTTCGTC 
0481 GCATACCATG GACTGCCCGT  
 in silico PCR Fragment 
0001 GGCCAGCTGG AGGATCTCTT CAAGAGTGCC AATGTGAAGA CCGTGCTCTT TGTGCCCTAC 
0061 GCTCTGCGGG ATCACGACAA GTACACTGCC ACCGTGCGCG ATGCACTGCA GCCGTGGGGA 
0121 TTCAATGTGG AGGGACTGCA CACGAAGCCG GATCGCGAGC AGGCATTGCG GGAGGCACAG 
0181 GCCATCTTTG TGGGCGGCGG CAACACCTTT GTGCTGCTGC GTTCGCTCTA CGAGATGAAG 
0241 TTGCTCGATC CGATTAGGGA ACTGGTGCTC CAGCGTGGAT TGCCTTACGT GGGCAGCAGT 
0301 GCGGGCACAA ATGTGGCAAC TCGATCCATT CACACCACCA ACGACATGCC GGTGGCCTAT 
0361 CCGCCGAGCT TTGAGGCACT CGCCCTCGTT CCCTTCAACA TCAATCCGCA CTATCTGGAT 
0421 CCGGAGGCGG GTAGTCGGCA CAAGGGCGAG ACGCGAGACG AGCGGCTGGA GGAGTTCGTC 
0481 GCATACCATG GACTGCCCGT  
 Assemble Data 

2200R-2.IR_full       1   GGCCAGCTGGAGGATCTCTTCAAGAGTGCCAATGTGAAGACCGTGCTCTTTGTGCCCTAC 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2200R-2.in silico     1   GGCCAGCTGGAGGATCTCTTCAAGAGTGCCAATGTGAAGACCGTGCTCTTTGTGCCCTAC 60


2200R-2.IR_full       61  GCTCTGCGGGATCACGACAAGTACACTGCCACCGTGCGCGATGCACTGCAGCCGTGGGGA 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2200R-2.in silico     61  GCTCTGCGGGATCACGACAAGTACACTGCCACCGTGCGCGATGCACTGCAGCCGTGGGGA 120


2200R-2.IR_full       121 TTCAATGTGGAGGGACTGCACACGAAGCCGGATCGCGAGCAGGCATTGCGGGAGGCACAG 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2200R-2.in silico     121 TTCAATGTGGAGGGACTGCACACGAAGCCGGATCGCGAGCAGGCATTGCGGGAGGCACAG 180


2200R-2.IR_full       181 GCCATCTTTGTGGGCGGCGGCAACACCTTTGTGCTGCTGCGTTCGCTCTACGAGATGAAG 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2200R-2.in silico     181 GCCATCTTTGTGGGCGGCGGCAACACCTTTGTGCTGCTGCGTTCGCTCTACGAGATGAAG 240


2200R-2.IR_full       241 TTGCTCGATCCGATTAGGGAACTGGTGCTCCAGCGTGGATTGCCTTACGTGGGCAGCAGT 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2200R-2.in silico     241 TTGCTCGATCCGATTAGGGAACTGGTGCTCCAGCGTGGATTGCCTTACGTGGGCAGCAGT 300


2200R-2.IR_full       301 GCGGGCACAAATGTGGCAACTCGATCCATTCACACCACCAACGACATGCCGGTGGCCTAT 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2200R-2.in silico     301 GCGGGCACAAATGTGGCAACTCGATCCATTCACACCACCAACGACATGCCGGTGGCCTAT 360


2200R-2.IR_full       361 CCGCCGAGCTTTGAGGCACTCGCCCTCGTTCCCTTCAACATCAATCCGCACTATCTGGAT 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2200R-2.in silico     361 CCGCCGAGCTTTGAGGCACTCGCCCTCGTTCCCTTCAACATCAATCCGCACTATCTGGAT 420


2200R-2.IR_full       421 CCGGAGGCGGGTAGTCGGCACAAGGGCGAGACGCGAGACGAGCGGCTGGAGGAGTTCGTC 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2200R-2.in silico     421 CCGGAGGCGGGTAGTCGGCACAAGGGCGAGACGCGAGACGAGCGGCTGGAGGAGTTCGTC 480


2200R-2.IR_full       481 GCATACCATGGACTGCCCGT 500
                          ||||||||||||||||||||
2200R-2.in silico     481 GCATACCATGGACTGCCCGT 500



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_132624.1  CG2200-RA (CG2200), mRNA 
0   0  4  3  4  NM_141542.1  CG11718-RA (CG11718), mRNA 
0   0  3  2  2  NM_137385.1  CG18432-RA (CG18432), mRNA 
0   0  2  2  5  NM_166894.1  CG11491-RA, transcript variant A (br), mRNA 
0   0  1  5  2  NM_170239.2  CG31092-RA, transcript variant A (LpR2), mRNA 
0   0  1  4  5  NM_167189.2  CG32705-RA (CG32705), mRNA 
0   0  1  3  5  NM_001042872.1  CG14042-RB, transcript variant B (CG14042), mRNA 
0   0  1  3  5  NM_001042871.1  CG14042-RA, transcript variant A (CG14042), mRNA 
0   0  1  3  5  NM_176520.1  CG33094-RB (Synd), mRNA 
0   0  1  3  5  NM_144359.2  CG14041-RB, transcript variant B (SP555), mRNA 
0   0  1  3  5  NM_164618.1  CG14041-RA, transcript variant A (SP555), mRNA 
0   0  1  3  5  NM_079930.2  CG3856-RB, transcript variant B (Oamb), mRNA 
0   0  1  3  4  NM_141936.1  CG6525-RA (CG6525), mRNA 
0   0  1  2  5  NM_137324.1  CG15614-RA (CG15614), mRNA 
0   0  1  2  4  NM_138090.1  CG12252-RA (CG12252), mRNA 
0   0  1  2  3  NM_144354.2  CG6987-RA (SF2), mRNA 
0   0  1  2  3  NM_167386.1  CG32626-RD, transcript variant D (CG32626), mRNA 
0   0  1  2  3  NM_167385.1  CG32626-RB, transcript variant B (CG32626), mRNA 
0   0  1  2  3  NM_132703.2  CG32626-RC, transcript variant C (CG32626), mRNA 
0   0  1  2  3  NM_167384.1  CG32626-RA, transcript variant A (CG32626), mRNA 
0   0  1  2  2  NM_166421.1  CG9364-RA, transcript variant A (Treh), mRNA 
0   0  1  2  2  NM_166425.1  CG9364-RB, transcript variant B (Treh), mRNA 
0   0  1  2  2  NM_166423.2  CG9364-RC, transcript variant C (Treh), mRNA 
0   0  1  2  2  NM_166424.1  CG9364-RF, transcript variant F (Treh), mRNA 
0   0  1  2  2  NM_080082.2  CG9364-RD, transcript variant D (Treh), mRNA 
0   0  1  2  2  NM_166422.1  CG9364-RE, transcript variant E (Treh), mRNA 
0   0  0  6  5  NM_139973.1  CG6694-RA (CG6694), mRNA 
0   0  0  5  9  NM_170139.1  CG31132-RA (BRWD3), mRNA 
0   0  0  5  2  NM_001043259.1  CG34157-RC, transcript variant C (Dys), mRNA 
0   0  0  5  2  NM_001043257.1  CG34157-RH, transcript variant H (Dys), mRNA 

SHIGEN - SHared Information of GENetic resources - National Institute of Genetics (NIG)
 Copyright (c) National Institute of Genetics (NIG)
All rights reserved.
The NIG-Fly is a part of the National BioResource Project which started in 2002.