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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
2186R-1 |
| Symbol |
CG2186 |
Full Name |
CG2186 |
| CG No |
CG2186 |
Old CG No |
CG2186 |
| Synonyms |
CG2186 |
| Accession No (Link to NCBI) |
NM_132432.1 |
| Inserted Chr. |
lll |
| Insertional Mutation |
lethal |
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624
[
PubMed ID = 21666802
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgCTCTCGGACGAAGAGCTCGAA |
| Primer Seq. 3' |
aatctagaggtaccGAGGCCAGTAGCGAGCACAAG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 CTCTCGGACG AAGAGCTCGA AAAGGATGTC ACCAGCGAGG TGAAGCAGCA GATGCGCNGC
0061 CGAGGCCGAG GAGTGGGCCC AGGAGCAGAT TAGACAGCAC GAGCGCAAGC ACAAGGAGCA
0121 ATTGCAGGCA GATGCTGACT GCAGCGACTG AAGTCACTGT TGCAGCCACG GCCACCGCAG
0181 AGGTAGCACC AACTTTAAGG GCGCAACCAG AGCTAGCTTC ATCTCCTGCT CCGGATCCTG
0241 TTCCTGCGAA ACCTCTAGTC GCTCAGGTGG AGAAACATGT TGACACAGCC CACGCGGCTG
0301 TGGCAGCTGA GCCAGCGAAA GAACTGGTGT CAGCCAGTCG CTCGGAATCC CCACAACAAC
0361 AAGTTAAGGA GGAGGCGCCT CCAGCTGATT GCGCCGAAAA GGAGACGACT CAGAATAATG
0421 GCAACAGCGA GGAACAAGCT GAGAACGCTG GCAGTCAGAG TGAGGCTGAA CGAGGACTGG
0481 GCTTGTGCTC GCTANCTGGC CTC |
| in silico PCR Fragment |
0001 CTCTCGGACG AAGAGCTCGA AAAGGATGTC ACCAGCGAGG TGAAGCAGCA GATGCGC-GC
0061 CGAGGCCGAG GAGTGGGCCC AGGAGCAGAT TAGACAGCAC GAGCGCAAGC ACAAGGAGCA
0121 ATTGCAGGCA GATGC-GACT GCAGCGACTG AAGTCACTGT TGCAGCCACG GCCACCGCAG
0181 AGGTAGCACC AACTTTAAGG GCGCAACCAG AGCTAGCTTC ATCTCCTGCT CCGGATCCTG
0241 TTCCTGCGAA ACCTCTAGTC GCTCAGGTGG AGAAACATGT TGACACAGCC CACGCGGCTG
0301 TGGCAGCTGA GCCAGCGAAG GAACTGGTGT CAGCCAGTCG CTCGGAATCC CCACAACAGC
0361 AAGCTAAGGA GGAGGCGCCT CCAGCTGATT GCGCCGAAAA GGAGGCGACT CAGAATAATG
0421 GCAACAGCGA GGAACAAGCT GAGAACGCTG GCAGTCAGAG TGAGGCTGAA CGAGGACTGG
0481 GCTTGTGCTC GCTA-CTGGC CTC |
| Assemble Data |
2186R-1.IR_full 1 CTCTCGGACGAAGAGCTCGAAAAGGATGTCACCAGCGAGGTGAAGCAGCAGATGCGCNGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||| ||
2186R-1.in silico 1 CTCTCGGACGAAGAGCTCGAAAAGGATGTCACCAGCGAGGTGAAGCAGCAGATGCGC-GC 60
2186R-1.IR_full 61 CGAGGCCGAGGAGTGGGCCCAGGAGCAGATTAGACAGCACGAGCGCAAGCACAAGGAGCA 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2186R-1.in silico 61 CGAGGCCGAGGAGTGGGCCCAGGAGCAGATTAGACAGCACGAGCGCAAGCACAAGGAGCA 120
2186R-1.IR_full 121 ATTGCAGGCAGATGCTGACTGCAGCGACTGAAGTCACTGTTGCAGCCACGGCCACCGCAG 180
||||||||||||||| ||||||||||||||||||||||||||||||||||||||||||||
2186R-1.in silico 121 ATTGCAGGCAGATGC-GACTGCAGCGACTGAAGTCACTGTTGCAGCCACGGCCACCGCAG 180
2186R-1.IR_full 181 AGGTAGCACCAACTTTAAGGGCGCAACCAGAGCTAGCTTCATCTCCTGCTCCGGATCCTG 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2186R-1.in silico 181 AGGTAGCACCAACTTTAAGGGCGCAACCAGAGCTAGCTTCATCTCCTGCTCCGGATCCTG 240
2186R-1.IR_full 241 TTCCTGCGAAACCTCTAGTCGCTCAGGTGGAGAAACATGTTGACACAGCCCACGCGGCTG 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2186R-1.in silico 241 TTCCTGCGAAACCTCTAGTCGCTCAGGTGGAGAAACATGTTGACACAGCCCACGCGGCTG 300
2186R-1.IR_full 301 TGGCAGCTGAGCCAGCGAAAGAACTGGTGTCAGCCAGTCGCTCGGAATCCCCACAACAAC 360
||||||||||||||||||| |||||||||||||||||||||||||||||||||||||| |
2186R-1.in silico 301 TGGCAGCTGAGCCAGCGAAGGAACTGGTGTCAGCCAGTCGCTCGGAATCCCCACAACAGC 360
2186R-1.IR_full 361 AAGTTAAGGAGGAGGCGCCTCCAGCTGATTGCGCCGAAAAGGAGACGACTCAGAATAATG 420
||| |||||||||||||||||||||||||||||||||||||||| |||||||||||||||
2186R-1.in silico 361 AAGCTAAGGAGGAGGCGCCTCCAGCTGATTGCGCCGAAAAGGAGGCGACTCAGAATAATG 420
2186R-1.IR_full 421 GCAACAGCGAGGAACAAGCTGAGAACGCTGGCAGTCAGAGTGAGGCTGAACGAGGACTGG 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2186R-1.in silico 421 GCAACAGCGAGGAACAAGCTGAGAACGCTGGCAGTCAGAGTGAGGCTGAACGAGGACTGG 480
2186R-1.IR_full 481 GCTTGTGCTCGCTANCTGGCCTC 503
|||||||||||||| ||||||||
2186R-1.in silico 481 GCTTGTGCTCGCTA-CTGGCCTC 503
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_132432.1 |
CG2186-RA (CG2186), mRNA |
| 0.2 |
1 |
2 |
2 |
3 |
NM_164869.1 |
CG18660-RA, transcript variant A (Nckx30C), mRNA |
| 0.2 |
1 |
2 |
2 |
3 |
NM_164870.1 |
CG18660-RB, transcript variant B (Nckx30C), mRNA |
| 0.2 |
1 |
2 |
2 |
3 |
NM_143754.2 |
CG18660-RC, transcript variant C (Nckx30C), mRNA |
| 0 |
0 |
5 |
3 |
20 |
NM_001032051.1 |
CG33715-RD, transcript variant D (Msp-300), mRNA |
| 0 |
0 |
5 |
3 |
11 |
NM_001032053.1 |
CG33715-RB, transcript variant B (Msp-300), mRNA |
| 0 |
0 |
4 |
5 |
14 |
NM_134718.4 |
CG31795-RA, transcript variant A (ia2), mRNA |
| 0 |
0 |
4 |
5 |
14 |
NM_164401.3 |
CG31795-RB, transcript variant B (ia2), mRNA |
| 0 |
0 |
3 |
8 |
7 |
NM_144011.1 |
CG14063-RA (CG14063), mRNA |
| 0 |
0 |
3 |
5 |
11 |
NM_001014552.1 |
CG15792-RD, transcript variant D (zip), mRNA |
| 0 |
0 |
3 |
5 |
11 |
NM_079136.2 |
CG15792-RA, transcript variant A (zip), mRNA |
| 0 |
0 |
3 |
5 |
11 |
NM_001014553.1 |
CG15792-RC, transcript variant C (zip), mRNA |
| 0 |
0 |
3 |
5 |
11 |
NM_166704.1 |
CG15792-RB, transcript variant B (zip), mRNA |
| 0 |
0 |
3 |
3 |
2 |
NM_142401.2 |
CG17806-RA (CG17806), mRNA |
| 0 |
0 |
2 |
4 |
5 |
NM_057512.3 |
CG3724-RA (Pgd), mRNA |
| 0 |
0 |
2 |
4 |
4 |
NM_142033.1 |
CG14376-RA (CG14376), mRNA |
| 0 |
0 |
2 |
4 |
4 |
NM_176557.1 |
CG33106-RB, transcript variant B (mask), mRNA |
| 0 |
0 |
2 |
4 |
4 |
NM_176556.1 |
CG33106-RA, transcript variant A (mask), mRNA |
| 0 |
0 |
2 |
2 |
8 |
NM_141334.1 |
CG10979-RA (CG10979), mRNA |
| 0 |
0 |
2 |
2 |
6 |
NM_132610.2 |
CG4404-RA (CG4404), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_139894.1 |
CG8492-RA (CG8492), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_133012.2 |
CG32560-RA (CG32560), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_057867.2 |
CG8808-RA (Pdk), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_169867.1 |
CG16718-RB, transcript variant B (CG16718), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_142563.1 |
CG16718-RA, transcript variant A (CG16718), mRNA |
| 0 |
0 |
1 |
9 |
4 |
NM_166688.1 |
CG30426-RA (CG30426), mRNA |
| 0 |
0 |
1 |
7 |
12 |
NM_079170.2 |
CG1066-RB, transcript variant B (Shab), mRNA |
| 0 |
0 |
1 |
7 |
12 |
NM_167967.1 |
CG1066-RA, transcript variant A (Shab), mRNA |
| 0 |
0 |
1 |
7 |
12 |
NM_001043115.1 |
CG1066-RC, transcript variant C (Shab), mRNA |
| 0 |
0 |
1 |
6 |
2 |
NM_135028.2 |
CG15625-RA (CG15625), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
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|
JNK signal
|
Caspase-3 signal
| N |
 |
| ++ |
|
| DB |
 |
| ++ |
|
| DC |
 |
| ++ |
|
| DM |
 |
| ++ |
|
| VM |
 |
| + |
|
| VB |
 |
| |
|
|
| Dcompartment |
|
Penetrance |
| + |
|
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