NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 2147R-2

Symbol CG2147 Full Name CG2147

CG No CG2147 Old CG No CG2147

Synonyms BcDNA:LP02728, CG2147

Accession No (Link to NCBI) NM_132237.2

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees viable

Map Viewer

Reference
[Please submit your publication] Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624 [ PubMed ID = 21666802 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgATGGATCCAACACCGCCGCTT

Primer Seq. 3'

aatctagaggtaccTTGGTTCTTGGTGGAACCTTG

Predicted Fragment Size

IR fragment full Seq

0001 ATGGATCCAA CACCGCCGCT TCGTGCCACC AATAGTATTA ACCCACAGCA GGTGGCAAGC 
0061 GTTCCACCGC CACCGCCAAC GGGGCAAATC ATAGCTCCAT CGGGAGCGTT TCCTAGGATC 
0121 CCTGACTCCC GGCTGGAATG ACCCGCCACC CATTTCGGCG GATTCTCAGA GCTGCAAATT 
0181 AAACAATAGT CGTCCACGCC TAGATCTTCG CANAGCGCGT GGCCCACCCG CTGGACGGGA 
0241 AGTCCTCNTA GTGTCCAGCT GGTTCAGCCA CAACTATCAA TACCTACAGC ACAAGATTCA 
0301 ATCTCGCCGC GTCCCGTGGC CCTGGTAACA CCCCAGCGCC AGATTCCATA TCCGGATCCC 
0361 AACAATCCAC CGGCCGGTGG AGCGGNATGT GGGCGGTTTC GGAGCGGGAC CGAAATGCAC 
0421 AATGCCGTGC GCCTGCCANC GTTGGCCCAC AGCATCAGCA TCGATNCCTC CAGGGTGGCC 
0481 AAgGTTCCAC CAAGAACCAA

in silico PCR Fragment

0001 ATGGATCCAA CACCGCCGCT TCGTGCCACC AATAGTATTA ACCCACAGCA GGTGGCAAGC 
0061 GTTCCACCGC CACCGCCAAC GGGGCAAATC ATAGCTCCAT CGGGAGCGTT TCCT-GGATC 
0121 CCTGACTCCC GGCTGGAATG ACCCGCCACC CATTTCGGCG GATTCTCAGA GCTGCAAATT 
0181 AAACAATAGT CGTCCACGCC TAGATCTTCG CA-AGCGCGT GGCCCACCCG CTGGACGGGA 
0241 AGTCCTC-TA GTGTCCAGCT GGTTCAGCCA CAACTATCAA TACCTACAGC ACAAGATTCA 
0301 ATCTCGCCGC GTCCCGTGGC CCTGGTAACA CCCCAGCGCC AGATTCCATA TCCGGATCCC 
0361 AACAATCCAC CGGCCGGTGG AGCGG-ATGT GGGCGGTTTC GGAGCGGGAC C-AAATGC-C 
0421 AATGCCGTGC GCCTGCCACC GTTGGCCCAC AGCATCAGCA TCGATCCCTC CAGGGTGGCC 
0481 AAGGTTCCAC CAAGAACCAA

Assemble Data


2147R-2.IR_full       1   ATGGATCCAACACCGCCGCTTCGTGCCACCAATAGTATTAACCCACAGCAGGTGGCAAGC 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2147R-2.in silico     1   ATGGATCCAACACCGCCGCTTCGTGCCACCAATAGTATTAACCCACAGCAGGTGGCAAGC 60


2147R-2.IR_full       61  GTTCCACCGCCACCGCCAACGGGGCAAATCATAGCTCCATCGGGAGCGTTTCCTAGGATC 120
                          |||||||||||||||||||||||||||||||||||||||||||||||||||||| |||||
2147R-2.in silico     61  GTTCCACCGCCACCGCCAACGGGGCAAATCATAGCTCCATCGGGAGCGTTTCCT-GGATC 120


2147R-2.IR_full       121 CCTGACTCCCGGCTGGAATGACCCGCCACCCATTTCGGCGGATTCTCAGAGCTGCAAATT 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2147R-2.in silico     121 CCTGACTCCCGGCTGGAATGACCCGCCACCCATTTCGGCGGATTCTCAGAGCTGCAAATT 180


2147R-2.IR_full       181 AAACAATAGTCGTCCACGCCTAGATCTTCGCANAGCGCGTGGCCCACCCGCTGGACGGGA 240
                          |||||||||||||||||||||||||||||||| |||||||||||||||||||||||||||
2147R-2.in silico     181 AAACAATAGTCGTCCACGCCTAGATCTTCGCA-AGCGCGTGGCCCACCCGCTGGACGGGA 240


2147R-2.IR_full       241 AGTCCTCNTAGTGTCCAGCTGGTTCAGCCACAACTATCAATACCTACAGCACAAGATTCA 300
                          ||||||| ||||||||||||||||||||||||||||||||||||||||||||||||||||
2147R-2.in silico     241 AGTCCTC-TAGTGTCCAGCTGGTTCAGCCACAACTATCAATACCTACAGCACAAGATTCA 300


2147R-2.IR_full       301 ATCTCGCCGCGTCCCGTGGCCCTGGTAACACCCCAGCGCCAGATTCCATATCCGGATCCC 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2147R-2.in silico     301 ATCTCGCCGCGTCCCGTGGCCCTGGTAACACCCCAGCGCCAGATTCCATATCCGGATCCC 360


2147R-2.IR_full       361 AACAATCCACCGGCCGGTGGAGCGGNATGTGGGCGGTTTCGGAGCGGGACCGAAATGCAC 420
                          ||||||||||||||||||||||||| ||||||||||||||||||||||||| |||||| |
2147R-2.in silico     361 AACAATCCACCGGCCGGTGGAGCGG-ATGTGGGCGGTTTCGGAGCGGGACC-AAATGC-C 420


2147R-2.IR_full       421 AATGCCGTGCGCCTGCCANCGTTGGCCCACAGCATCAGCATCGATNCCTCCAGGGTGGCC 480
                          |||||||||||||||||| |||||||||||||||||||||||||| ||||||||||||||
2147R-2.in silico     421 AATGCCGTGCGCCTGCCACCGTTGGCCCACAGCATCAGCATCGATCCCTCCAGGGTGGCC 480


2147R-2.IR_full       481 AAGGTTCCACCAAGAACCAA 500
                          ||||||||||||||||||||
2147R-2.in silico     481 AAGGTTCCACCAAGAACCAA 500

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	476	0	2	5	NM_132237.2	CG2147-RA (CG2147), mRNA
0	0	3	2	2	NM_137253.2	CG8446-RA (CG8446), mRNA
0	0	2	4	3	NM_057617.3	CG10497-RA, transcript variant A (Sdc), mRNA
0	0	2	3	4	NM_139949.2	CG7185-RA (CG7185), mRNA
0	0	2	2	5	NM_132086.1	CG15894-RA (CG15894), mRNA
0	0	1	7	4	NM_134978.2	CG16857-RA (CG16857), mRNA
0	0	1	5	10	NM_165738.1	CG2269-RC, transcript variant C (CG2269), mRNA
0	0	1	5	10	NM_136709.2	CG2269-RA, transcript variant A (CG2269), mRNA
0	0	1	5	10	NM_165737.1	CG2269-RB, transcript variant B (CG2269), mRNA
0	0	1	4	12	NM_175956.1	CG33003-RA (CG33003), mRNA
0	0	1	4	6	NM_167916.2	CG32315-RA (dlt), mRNA
0	0	1	3	11	NM_167487.1	CG32577-RA (disco-r), mRNA
0	0	1	3	3	NM_078722.2	CG17941-RA (ds), mRNA
0	0	1	3	2	NM_142497.2	CG18208-RA (CG18208), mRNA
0	0	1	2	5	NM_132719.2	CG32604-RA, transcript variant A (l(1)G0007), mRNA
0	0	1	2	4	NM_141178.3	CG12581-RA, transcript variant A (CG12581), mRNA
0	0	1	2	4	NM_164313.1	CG12581-RB, transcript variant B (CG12581), mRNA
0	0	1	2	3	NM_079436.1	CG12581-RB, transcript variant B (CG12581), mRNA, small, or homeotic discs 1 CG8887-RA (ash1), mRNA
0	0	1	2	2	NM_058010.3	CG8200-RA, transcript variant A (Flo), mRNA
0	0	1	2	2	NM_166102.1	CG8200-RB, transcript variant B (Flo), mRNA
0	0	1	2	2	NM_140029.2	CG4942-RA (CG4942), mRNA
0	0	0	8	5	NM_166484.1	CG17952-RC, transcript variant C (LBR), mRNA
0	0	0	8	5	NM_137764.2	CG17952-RA, transcript variant A (LBR), mRNA
0	0	0	8	5	NM_166485.1	CG17952-RB, transcript variant B (LBR), mRNA
0	0	0	7	10	NM_169783.1	CG31243-RB, transcript variant B (cpo), mRNA
0	0	0	7	10	NM_169781.1	CG31243-RE, transcript variant E (cpo), mRNA
0	0	0	7	10	NM_169782.1	CG31243-RA, transcript variant A (cpo), mRNA
0	0	0	7	3	NM_080105.2	CG31243-RF, transcript variant F (cpo), mRNA
0	0	0	5	6	NM_168517.1	CG11006-RC, transcript variant C (CG11006), mRNA
0	0	0	5	6	NM_168518.1	CG11006-RB, transcript variant B (CG11006), mRNA

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