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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
|
| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
2125R-1 |
| Symbol |
ci |
Full Name |
cubitus interruptus |
| CG No |
CG2125 |
Old CG No |
CG2125 |
| Synonyms |
Gli, Ci, CI, CG2125, ci155, CID, CiD, ciD, l(4)17, ci[D], ci-D, Ci[D], Ce, l(4)13, l(4)102ABc, ci, Ci/GLI |
| Accession No (Link to NCBI) |
NM_079878.3 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Gao Y, Shan Z, Jian C, Wang Y, Yao X, Li S, Ti X, Zhao G, Liu C, Zhang Q.
HIB/SPOP inhibits Ci/Gli-mediated tumorigenesis by modulating the RNA Polymerase II components stabilities.
iScience (2023) 26(8) 107334
[
PubMed ID = 37554435
]
[
RRC reference
]
Liu B, Ding Y, Sun B, Liu Q, Zhou Z, Zhan M.
The Hh pathway promotes cell apoptosis through Ci-Rdx-Diap1 axis.
Cell Death Discov (2021) 7(1) 263
[
PubMed ID = 34561426
]
[
RRC reference
]
Velarde SB, Quevedo A, Estella C, Baonza A.
Dpp and Hedgehog promote the glial response to neuronal apoptosis in the developing Drosophila visual system.
PLoS Biol (2021) 19(8) e3001367
[
PubMed ID = 34379617
]
[
RRC reference
]
Cho Y, Lai CM, Lin KY, Hsu HJ.
A Targeted RNAi Screen Reveals Drosophila Female-Sterile Genes That Control the Size of Germline Stem Cell Niche During Development.
G3 (Bethesda) (2018) 8(7) 2345-2354
[
PubMed ID = 29764959
]
[
RRC reference
]
Lai CM, Lin KY, Kao SH, Chen YN, Huang F, Hsu HJ.
Hedgehog signaling establishes precursors for germline stem cell niches by regulating cell adhesion.
J Cell Biol (2017) 216(5) 1439-1453
[
PubMed ID = 28363970
]
[
RRC reference
]
Zhang Z, Lv X, Jiang J, Zhang L, Zhao Y.
Dual roles of Hh signaling in the regulation of somatic stem cell self-renewal and germline stem cell maintenance in Drosophila testis.
Cell Res (2013) 23(4) 573-6
[
PubMed ID = 23419515
]
[
RRC reference
]
Han H, Pan C, Liu C, Lv X, Yang X, Xiong Y, Lu Y, Wu W, Han J, Zhou Z, Jiang H, Zhang L, Zhao Y.
Gut-neuron interaction via Hh signaling regulates intestinal progenitor cell differentiation in Drosophila.
Cell Discov (2015) 1 15006
[
PubMed ID = 27462407
]
[
RRC reference
]
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Bauke AC, Sasse S, Matzat T, Klämbt C.
A transcriptional network controlling glial development in the Drosophila visual system.
Development (2015) 142(12) 2184-93
[
PubMed ID = 26015542
]
[
RRC reference
]
Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340
[
PubMed ID = 25473846
]
[
RRC reference
]
Zhang Z, Feng J, Pan C, Lv X, Wu W, Zhou Z, Liu F, Zhang L, Zhao Y.
Atrophin-Rpd3 complex represses Hedgehog signaling by acting as a corepressor of CiR.
J Cell Biol (2013) 203(4) 575-83
[
PubMed ID = 24385484
]
[
RRC reference
]
Sato T, Ogata J, Niki Y.
BMP and Hh signaling affects primordial germ cell division in Drosophila.
Zoolog Sci (2010) 27(10) 804-10
[
PubMed ID = 20887178
]
[
RRC reference
]
Terriente-Félix A, Molnar C, Gómez-Skarmeta JL, de Celis JF.
A conserved function of the chromatin ATPase Kismet in the regulation of hedgehog expression.
Dev Biol (2011) 350(2) 382-92
[
PubMed ID = 21146514
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGAAATGGACGCCTACGCGTTA |
| Primer Seq. 3' |
aatctagaggtaccCAGCAGCATTATGCAGTTCAC |
| Predicted Fragment Size |
523 |
| IR fragment full Seq |
0001 GAAATGNGAC GCCTACGCGT TACCTACATA TTTTCCTCTT GCGTATTCTG AATTGCAGTT
0061 TTTAGCGTCC AGGAGAGCAG CTGCCGTCGC TGCAGCGGCT ACTGTTTTAC CAGGATCACC
0121 ATGCATAAAC CAACATCACC CAACTGACGT TTCAAGCTCG GTAACAGTGC CATCAATTAT
0181 TCCAACGGGT GGAACATCAG ATTCAATTAA AACTTCAATA CAACCACAAA TATGCAATGA
0241 AAACACCCTT CTTGGAAATG CTGGCCACCA GCACAATCAT CAGCCTCAAC ATGTTCACAA
0301 TATAAATGTT ACTGGACAGC CACATGACTT TCACCCGGCC TATAGAATTC CCGGATACAT
0361 GGAACAGTTG TATTCTCTTC AACGAACTAA TTCAGCTTCA TCTTTTCACG ATCCCTATGT
0421 CAATTGTGCA TCAGCATTCC ATCTTGCCGG ACTTGGTTTG GGATCAGCTG ATTTTTTGGG
0481 CAGCCGAGGT TTGAGCTCTT TGGGTGAACT GCATAATGCT GCTG |
| in silico PCR Fragment |
0001 GAAATG-GAC GCCTACGCGT TACCTACATA TTTTCCTCTT GCGTATTCTG AATTGCAGTT
0061 TTTAGCGTCC AGGAGAGCAG CTGCCGTCGC TGCAGCGGCT ACTGTTTTAC CAGGATCACC
0121 ATGCATAAAC CAACATCACC CAACTGACGT TTCAAGCTCG GTAACAGTGC CATCAATTAT
0181 TCCAACGGGT GGAACATCAG ATTCAATTAA AACTTCAATA CAACCACAAA TATGCAATGA
0241 AAACACCCTT CTTGGAAATG CTGGCCACCA GCACAATCAT CAGCCTCAAC ATGTTCACAA
0301 TATAAATGTT ACTGGACAGC CACATGACTT TCACCCGGCC TATAGAATTC CCGGATACAT
0361 GGAACAGTTG TATTCTCTTC AACGAACTAA TTCAGCTTCA TCTTTTCACG ATCCCTATGT
0421 CAATTGTGCA TCAGCATTCC ATCTTGCCGG ACTTGGTTTG GGATCAGCTG ATTTTTTGGG
0481 CAGCCGAGGT TTGAGCTCTT TGGGTGAACT GCATAATGCT GCTG |
| Assemble Data |
2125R-1.IR_full 1 GAAATGNGACGCCTACGCGTTACCTACATATTTTCCTCTTGCGTATTCTGAATTGCAGTT 60
|||||| |||||||||||||||||||||||||||||||||||||||||||||||||||||
2125R-1.in silico 1 GAAATG-GACGCCTACGCGTTACCTACATATTTTCCTCTTGCGTATTCTGAATTGCAGTT 60
2125R-1.IR_full 61 TTTAGCGTCCAGGAGAGCAGCTGCCGTCGCTGCAGCGGCTACTGTTTTACCAGGATCACC 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2125R-1.in silico 61 TTTAGCGTCCAGGAGAGCAGCTGCCGTCGCTGCAGCGGCTACTGTTTTACCAGGATCACC 120
2125R-1.IR_full 121 ATGCATAAACCAACATCACCCAACTGACGTTTCAAGCTCGGTAACAGTGCCATCAATTAT 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2125R-1.in silico 121 ATGCATAAACCAACATCACCCAACTGACGTTTCAAGCTCGGTAACAGTGCCATCAATTAT 180
2125R-1.IR_full 181 TCCAACGGGTGGAACATCAGATTCAATTAAAACTTCAATACAACCACAAATATGCAATGA 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2125R-1.in silico 181 TCCAACGGGTGGAACATCAGATTCAATTAAAACTTCAATACAACCACAAATATGCAATGA 240
2125R-1.IR_full 241 AAACACCCTTCTTGGAAATGCTGGCCACCAGCACAATCATCAGCCTCAACATGTTCACAA 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2125R-1.in silico 241 AAACACCCTTCTTGGAAATGCTGGCCACCAGCACAATCATCAGCCTCAACATGTTCACAA 300
2125R-1.IR_full 301 TATAAATGTTACTGGACAGCCACATGACTTTCACCCGGCCTATAGAATTCCCGGATACAT 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2125R-1.in silico 301 TATAAATGTTACTGGACAGCCACATGACTTTCACCCGGCCTATAGAATTCCCGGATACAT 360
2125R-1.IR_full 361 GGAACAGTTGTATTCTCTTCAACGAACTAATTCAGCTTCATCTTTTCACGATCCCTATGT 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2125R-1.in silico 361 GGAACAGTTGTATTCTCTTCAACGAACTAATTCAGCTTCATCTTTTCACGATCCCTATGT 420
2125R-1.IR_full 421 CAATTGTGCATCAGCATTCCATCTTGCCGGACTTGGTTTGGGATCAGCTGATTTTTTGGG 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2125R-1.in silico 421 CAATTGTGCATCAGCATTCCATCTTGCCGGACTTGGTTTGGGATCAGCTGATTTTTTGGG 480
2125R-1.IR_full 481 CAGCCGAGGTTTGAGCTCTTTGGGTGAACTGCATAATGCTGCTG 524
||||||||||||||||||||||||||||||||||||||||||||
2125R-1.in silico 481 CAGCCGAGGTTTGAGCTCTTTGGGTGAACTGCATAATGCTGCTG 524
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
505 |
0 |
0 |
0 |
NM_079878.3 |
CG2125-RA (ci), mRNA |
| 0 |
0 |
4 |
3 |
5 |
NM_079700.1 |
CG3723-RA (Dhc93AB), mRNA |
| 0 |
0 |
3 |
17 |
14 |
NM_132412.1 |
CG9817-RA (CG9817), mRNA |
| 0 |
0 |
3 |
4 |
11 |
NM_132640.2 |
CG1770-RA, transcript variant A (HDAC4), mRNA |
| 0 |
0 |
3 |
4 |
11 |
NM_001014736.1 |
CG1770-RC, transcript variant C (HDAC4), mRNA |
| 0 |
0 |
3 |
4 |
11 |
NM_167356.1 |
CG1770-RB, transcript variant B (HDAC4), mRNA |
| 0 |
0 |
2 |
7 |
5 |
NM_134890.2 |
CG17265-RA (CG17265), mRNA |
| 0 |
0 |
2 |
6 |
5 |
NM_135186.1 |
CG16947-RA (CG16947), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_141676.2 |
CG12950-RA (CG12950), mRNA |
| 0 |
0 |
1 |
6 |
7 |
NM_136646.1 |
CG13953-RA (CG13953), mRNA |
| 0 |
0 |
1 |
5 |
4 |
NM_176036.1 |
CG32973-RA (CG32973), mRNA |
| 0 |
0 |
1 |
4 |
6 |
NM_079416.2 |
CG4166-RA (not), mRNA |
| 0 |
0 |
1 |
4 |
4 |
NM_170570.1 |
CG1873-RB, transcript variant B (Ef1alpha100E), mRNA |
| 0 |
0 |
1 |
4 |
4 |
NM_206592.1 |
CG1873-RD, transcript variant D (Ef1alpha100E), mRNA |
| 0 |
0 |
1 |
4 |
4 |
NM_079872.4 |
CG1873-RA, transcript variant A (Ef1alpha100E), mRNA |
| 0 |
0 |
1 |
4 |
4 |
NM_206593.1 |
CG1873-RC, transcript variant C (Ef1alpha100E), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_137507.3 |
CG5341-RA (sec6), mRNA |
| 0 |
0 |
1 |
3 |
7 |
NM_134964.1 |
CG3921-RA (CG3921), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_138052.2 |
CG3356-RA (CG3356), mRNA |
| 0 |
0 |
0 |
7 |
7 |
NM_141458.2 |
CG32466-RA, transcript variant A (rn), mRNA |
| 0 |
0 |
0 |
6 |
4 |
NM_169170.1 |
CG1070-RC, transcript variant C (Alh), mRNA |
| 0 |
0 |
0 |
6 |
3 |
NM_169171.1 |
CG1070-RD, transcript variant D (Alh), mRNA |
| 0 |
0 |
0 |
6 |
3 |
NM_079526.2 |
CG1070-RA, transcript variant A (Alh), mRNA |
| 0 |
0 |
0 |
6 |
3 |
NM_169172.1 |
CG1070-RB, transcript variant B (Alh), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_057338.2 |
CG8246-RA (Poxn), mRNA |
| 0 |
0 |
0 |
5 |
2 |
NM_168131.1 |
CG5505-RA, transcript variant A (mule), mRNA |
| 0 |
0 |
0 |
5 |
2 |
NM_168132.1 |
CG5505-RD, transcript variant D (mule), mRNA |
| 0 |
0 |
0 |
5 |
2 |
NM_139729.2 |
CG5505-RE, transcript variant E (mule), mRNA |
| 0 |
0 |
0 |
5 |
2 |
NM_168135.1 |
CG5505-RB, transcript variant B (mule), mRNA |
| 0 |
0 |
0 |
5 |
2 |
NM_168133.1 |
CG5505-RF, transcript variant F (mule), mRNA |
|
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