|
Notice:
|
|
Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
|
|
| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
discarded
|
| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
2098R-2 |
| Symbol |
ferrochelatase |
Full Name |
ferrochelatase |
| CG No |
CG2098 |
Old CG No |
CG2098 |
| Synonyms |
CG2098, ferrochelatase |
| Accession No (Link to NCBI) |
NM_079874.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Ohhara Y, Nakamura A, Kato Y, Yamakawa-Kobayashi K.
Chaperonin TRiC/CCT supports mitotic exit and entry into endocycle in Drosophila.
PLoS Genet (2019) 15(4) e1008121
[
PubMed ID = 31034473
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgACACGGACCAGGTGCATGACT |
| Primer Seq. 3' |
aatctagaggtaccTTAGAAGTGGATGAGTGCCCC |
| Predicted Fragment Size |
459 |
| IR fragment full Seq |
0001 ACACGGACCA GGTGCATGAC TACCTCCTGC GTATTATGAC CGACCGGGAC ATGATCCANG
0061 CTGCCGGTGC AGNAGCCGTC TTGGTCCGTG GATCGCCCAG CGCAGGACGC CCGAGGTACA
0121 GAAGAAGTAC AAGGAGATCG GCGGTGGTTC ACCGATTCTT AAGTGGACCG AGCTGCAGGG
0181 CCAGCTCATG TGCGAGCAGT TGGACAGAAT CTCCCCGGAG ACAGCACCTC ACAAGCACTA
0241 CGTCGGTTTC CGCTACGTGA ATCCCCTCAC TGAGAACACG CTGGCCGAGA TCGAAAAGGA
0301 CAAACCAGAG CGCGTTGTAC TCTTTTCACA ATATCCACAG TACAGCTGCG CCACCTCCGG
0361 ATCCAGCTTT AACTCCATAT TTACTCATTA TCGGAGCAAT AATCTGCCCT CGGATATCAA
0421 ATGGAGCATT ATCGACAGGT GGGGCACTCA TCCACTTCTA A |
| in silico PCR Fragment |
0001 ACACGGACCA GGTGCATGAC TACCTCCTGC GTATTATGAC CGACCGGGAC ATGATCCA-G
0061 CTGCCGGTGC AG-AGCCGTC TTGGTCCGTG GATCGCCCAG CGCAGGACGC CCGAGGTACA
0121 GAAGAAGTAC AAGGAGATCG GCGGTGGTTC ACCGATTCTT AAGTGGACCG AGCTGCAGGG
0181 CCAGCTCATG TGCGAGCAGT TGGACAGAAT CTCCCCGGAG ACAGCACCTC ACAAGCACTA
0241 CGTCGGTTTC CGCTACGTGA ATCCCCTCAC TGAGAACACG CTGGCCGAGA TCGAAAAGGA
0301 CAAACCAGAG CGCGTTGTAC TCTTTTCACA ATATCCACAG TACAGCTGCG CCACCTCCGG
0361 ATCCAGCTTT AACTCCATAT TTACTCATTA TCGGAGCAAT AATCTGCCCT CGGATATCAA
0421 ATGGAGCATT ATCGACAGGT GGGGCACTCA TCCACTTCTA A |
| Assemble Data |
2098R-2.IR_full 1 ACACGGACCAGGTGCATGACTACCTCCTGCGTATTATGACCGACCGGGACATGATCCANG 60
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||| |
2098R-2.in silico 1 ACACGGACCAGGTGCATGACTACCTCCTGCGTATTATGACCGACCGGGACATGATCCA-G 60
2098R-2.IR_full 61 CTGCCGGTGCAGNAGCCGTCTTGGTCCGTGGATCGCCCAGCGCAGGACGCCCGAGGTACA 120
|||||||||||| |||||||||||||||||||||||||||||||||||||||||||||||
2098R-2.in silico 61 CTGCCGGTGCAG-AGCCGTCTTGGTCCGTGGATCGCCCAGCGCAGGACGCCCGAGGTACA 120
2098R-2.IR_full 121 GAAGAAGTACAAGGAGATCGGCGGTGGTTCACCGATTCTTAAGTGGACCGAGCTGCAGGG 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2098R-2.in silico 121 GAAGAAGTACAAGGAGATCGGCGGTGGTTCACCGATTCTTAAGTGGACCGAGCTGCAGGG 180
2098R-2.IR_full 181 CCAGCTCATGTGCGAGCAGTTGGACAGAATCTCCCCGGAGACAGCACCTCACAAGCACTA 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2098R-2.in silico 181 CCAGCTCATGTGCGAGCAGTTGGACAGAATCTCCCCGGAGACAGCACCTCACAAGCACTA 240
2098R-2.IR_full 241 CGTCGGTTTCCGCTACGTGAATCCCCTCACTGAGAACACGCTGGCCGAGATCGAAAAGGA 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2098R-2.in silico 241 CGTCGGTTTCCGCTACGTGAATCCCCTCACTGAGAACACGCTGGCCGAGATCGAAAAGGA 300
2098R-2.IR_full 301 CAAACCAGAGCGCGTTGTACTCTTTTCACAATATCCACAGTACAGCTGCGCCACCTCCGG 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2098R-2.in silico 301 CAAACCAGAGCGCGTTGTACTCTTTTCACAATATCCACAGTACAGCTGCGCCACCTCCGG 360
2098R-2.IR_full 361 ATCCAGCTTTAACTCCATATTTACTCATTATCGGAGCAATAATCTGCCCTCGGATATCAA 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2098R-2.in silico 361 ATCCAGCTTTAACTCCATATTTACTCATTATCGGAGCAATAATCTGCCCTCGGATATCAA 420
2098R-2.IR_full 421 ATGGAGCATTATCGACAGGTGGGGCACTCATCCACTTCTAA 461
|||||||||||||||||||||||||||||||||||||||||
2098R-2.in silico 421 ATGGAGCATTATCGACAGGTGGGGCACTCATCCACTTCTAA 461
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
441 |
0 |
0 |
0 |
NM_170579.2 |
CG2098-RC, transcript variant C (ferrochelatase), mRNA |
| 100 |
441 |
0 |
0 |
0 |
NM_079874.2 |
CG2098-RA, transcript variant A (ferrochelatase), mRNA |
| 95.91 |
423 |
3 |
2 |
2 |
NM_170580.2 |
CG2098-RB, transcript variant B (ferrochelatase), mRNA |
| 0 |
0 |
6 |
4 |
3 |
NM_079584.2 |
CG4082-RA (Mcm5), mRNA |
| 0 |
0 |
3 |
3 |
2 |
NM_170027.2 |
CG31158-RA, transcript variant A (CG31158), mRNA |
| 0 |
0 |
3 |
3 |
2 |
NM_176541.1 |
CG31158-RB, transcript variant B (CG31158), mRNA |
| 0 |
0 |
3 |
2 |
7 |
NM_141688.1 |
CG8484-RA (topi), mRNA |
| 0 |
0 |
3 |
2 |
2 |
NM_141075.1 |
CG7634-RA (CG7634), mRNA |
| 0 |
0 |
2 |
4 |
2 |
NM_137707.2 |
CG30388-RA (Magi), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_144442.2 |
CG18854-RC, transcript variant C (CG18854), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_164872.1 |
CG18854-RA, transcript variant A (CG18854), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_164873.1 |
CG18854-RB, transcript variant B (CG18854), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_135469.2 |
CG18854-RB, transcript variant B (CG18854), mRNA,4,5-triphosphate kinase 1 CG4026-RA (IP3K1), mRNA |
| 0 |
0 |
1 |
5 |
2 |
NM_132628.2 |
CG15717-RA (CG15717), mRNA |
| 0 |
0 |
1 |
4 |
5 |
NM_137150.2 |
CG10209-RA (CG10209), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_167365.1 |
CG1640-RB, transcript variant B (CG1640), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_167366.1 |
CG1640-RC, transcript variant C (CG1640), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_167367.1 |
CG1640-RD, transcript variant D (CG1640), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_167368.1 |
CG1640-RE, transcript variant E (CG1640), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_132651.2 |
CG1640-RA, transcript variant A (CG1640), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_167369.1 |
CG1640-RF, transcript variant F (CG1640), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_135727.1 |
CG5418-RA (CG5418), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_137370.3 |
CG14478-RB, transcript variant B (CG14478), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_166229.2 |
CG14478-RA, transcript variant A (CG14478), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_130706.2 |
CG10802-RA (CG10802), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_131942.1 |
CG3527-RA (CG3527), mRNA |
| 0 |
0 |
0 |
6 |
6 |
NM_206496.1 |
CG14869-RB, transcript variant B (CG14869), mRNA |
| 0 |
0 |
0 |
6 |
6 |
NM_169659.2 |
CG14869-RA, transcript variant A (CG14869), mRNA |
| 0 |
0 |
0 |
6 |
3 |
NM_164438.1 |
CG31936-RA (Gr22e), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_130709.1 |
CG14271-RB (Gas8), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
|
JNK signal
|
Caspase-3 signal
|
|
|
|
|
