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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
2055R-1 |
| Symbol |
Pkn |
Full Name |
Protein kinase related to protein kinase N |
| CG No |
CG2049 |
Old CG No |
CG2055 |
| Synonyms |
PKN, CG2049, Dpkn, dPKN, l(2)45Ca, l(2)06736, DPKN, 3-2, PRK2, CT6660, CT6637, CG2055, v(2)rG232, Pk?3, Pk45C, Pkn, Protein kinase-like 45C, Protein kinase related to protein kinase N, protein kinase N, protein kinase c related |
| Accession No (Link to NCBI) |
NM_176110.1 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Hirschhäuser A, van Cann M, Bogdan S.
CK1α protects WAVE from degradation to regulate cell shape and motility in the immune response.
J Cell Sci (2021) 134(23)
[
PubMed ID = 34730182
]
[
RRC reference
]
Park J, Lee J, Kim JH, Lee J, Park H, Lim C.
ZNF598 co-translationally titrates poly(GR) protein implicated in the pathogenesis of C9ORF72-associated ALS/FTD.
Nucleic Acids Res (2021) 49(19) 11294-11311
[
PubMed ID = 34551427
]
[
RRC reference
]
Li Y, Sun X, Gao D, Ding Y, Liu J, Chen J, Luo J, Zhang J, Liu Q, Zhou Z.
Dual functions of Rack1 in regulating Hedgehog pathway.
Cell Death Differ (2020) 27(11) 3082-3096
[
PubMed ID = 32467643
]
[
RRC reference
]
Parsons LM, Grzeschik NA, Amaratunga K, Burke P, Quinn LM, Richardson HE.
A Kinome RNAi Screen in Drosophila Identifies Novel Genes Interacting with Lgl, aPKC, and Crb Cell Polarity Genes in Epithelial Tissues.
G3 (Bethesda) (2017) 7(8) 2497-2509
[
PubMed ID = 28611255
]
[
RRC reference
]
Hosono C, Matsuda R, Adryan B, Samakovlis C.
Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes.
Nat Cell Biol (2015) 17(12) 1569-76
[
PubMed ID = 26551273
]
[
RRC reference
]
Yashiro H, Loza AJ, Skeath JB, Longmore GD.
Rho1 regulates adherens junction remodeling by promoting recycling endosome formation through activation of myosin II.
Mol Biol Cell (2014) 25(19) 2956-69
[
PubMed ID = 25079692
]
[
RRC reference
]
Warner SJ, Longmore GD.
Distinct functions for Rho1 in maintaining adherens junctions and apical tension in remodeling epithelia.
J Cell Biol (2009) 185(6) 1111-25
[
PubMed ID = 19506041
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgAGCATCCCGTTCTGTACGAGC |
| Primer Seq. 3' |
aatctagaggtaccCTGGATCATGTTTTCCGCCCC |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 AGCATCCCGT TCTGTACGAG CTCAGTCACA AATATGGTTT CACAGAGAAT CTGCCGGAGA
0061 GCTGTATGTC CATACGGCTG GAGGAGATCA AGGAGGCCAT TCGGCGAGAG ATCCGCAAGG
0121 AGCTGAAGAT CAAAGAGGGC GCCGAGAAGC TCCGCGAGGT GGCTAAAGAT CGACGATCCC
0181 TCAGCGATGT GGCCGTTCTT GTCAAGAAGA GCAAAAGTAA ACTTGCCGAG CTGAAGTCCG
0241 AGTTGCAGGA GCTCGAGAGT CAAATCCTCC TGACATCGGC CAACACCGCC GTCAATAGTA
0301 ATGGACAAGA ATCGATCACT GCCTGCATTG ATCCCAATGG CGGCTTCTTG GTCAGCGGTG
0361 CAGTTGGTGG CTTGGGCGGT GGAAACACGG CTCTGGAGGG CGGCGCACCG GCCACTGCCA
0421 ATGACAAAGT GCTCGCCTCG CTGGAGAAGC AGCTGCAGAT CGAGATGAAG GTGAAGACCG
0481 GGGCGGAAAA CATGATCCAG |
| in silico PCR Fragment |
0001 AGCATCCCGT TCTGTACGAG CTCAGTCACA AATATGGTTT CACAGAGAAT CTGCCGGAGA
0061 GCTGTATGTC CATACGGCTG GAGGAGATCA AGGAGGCCAT TCGGCGAGAG ATCCGCAAGG
0121 AGCTGAAGAT CAAAGAGGGC GCCGAGAAGC TCCGCGAGGT GGCTAAAGAT CGACGATCCC
0181 TCAGCGATGT GGCCGTTCTT GTCAAGAAGA GCAAAAGTAA ACTTGCCGAG CTGAAGTCCG
0241 AGTTGCAGGA GCTCGAGAGT CAAATCCTCC TGACATCGGC CAACACCGCC GTCAATAGTA
0301 ATGGACAAGA ATCGATCACT GCCTGCATTG ATCCCAATGG CGGCTTCTTG GTCAGCGGTG
0361 CAGTTGGTGG CTTGGGCGGC GGAAACACGG CTCTGGAGGG CGGCGCACCG GCCACTGCCA
0421 ATGACAAAGT GCTCGCCTCG CTGGAGAAGC AGCTGCAGAT CGAGATGAAG GTGAAGACCG
0481 GGGCGGAAAA CATGATCCAG |
| Assemble Data |
2055R-1.IR_full 1 AGCATCCCGTTCTGTACGAGCTCAGTCACAAATATGGTTTCACAGAGAATCTGCCGGAGA 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2055R-1.in silico 1 AGCATCCCGTTCTGTACGAGCTCAGTCACAAATATGGTTTCACAGAGAATCTGCCGGAGA 60
2055R-1.IR_full 61 GCTGTATGTCCATACGGCTGGAGGAGATCAAGGAGGCCATTCGGCGAGAGATCCGCAAGG 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2055R-1.in silico 61 GCTGTATGTCCATACGGCTGGAGGAGATCAAGGAGGCCATTCGGCGAGAGATCCGCAAGG 120
2055R-1.IR_full 121 AGCTGAAGATCAAAGAGGGCGCCGAGAAGCTCCGCGAGGTGGCTAAAGATCGACGATCCC 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2055R-1.in silico 121 AGCTGAAGATCAAAGAGGGCGCCGAGAAGCTCCGCGAGGTGGCTAAAGATCGACGATCCC 180
2055R-1.IR_full 181 TCAGCGATGTGGCCGTTCTTGTCAAGAAGAGCAAAAGTAAACTTGCCGAGCTGAAGTCCG 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2055R-1.in silico 181 TCAGCGATGTGGCCGTTCTTGTCAAGAAGAGCAAAAGTAAACTTGCCGAGCTGAAGTCCG 240
2055R-1.IR_full 241 AGTTGCAGGAGCTCGAGAGTCAAATCCTCCTGACATCGGCCAACACCGCCGTCAATAGTA 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2055R-1.in silico 241 AGTTGCAGGAGCTCGAGAGTCAAATCCTCCTGACATCGGCCAACACCGCCGTCAATAGTA 300
2055R-1.IR_full 301 ATGGACAAGAATCGATCACTGCCTGCATTGATCCCAATGGCGGCTTCTTGGTCAGCGGTG 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2055R-1.in silico 301 ATGGACAAGAATCGATCACTGCCTGCATTGATCCCAATGGCGGCTTCTTGGTCAGCGGTG 360
2055R-1.IR_full 361 CAGTTGGTGGCTTGGGCGGTGGAAACACGGCTCTGGAGGGCGGCGCACCGGCCACTGCCA 420
||||||||||||||||||| ||||||||||||||||||||||||||||||||||||||||
2055R-1.in silico 361 CAGTTGGTGGCTTGGGCGGCGGAAACACGGCTCTGGAGGGCGGCGCACCGGCCACTGCCA 420
2055R-1.IR_full 421 ATGACAAAGTGCTCGCCTCGCTGGAGAAGCAGCTGCAGATCGAGATGAAGGTGAAGACCG 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2055R-1.in silico 421 ATGACAAAGTGCTCGCCTCGCTGGAGAAGCAGCTGCAGATCGAGATGAAGGTGAAGACCG 480
2055R-1.IR_full 481 GGGCGGAAAACATGATCCAG 500
||||||||||||||||||||
2055R-1.in silico 481 GGGCGGAAAACATGATCCAG 500
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
4 |
NM_176111.1 |
CG2049-RC, transcript variant C (Pkn), mRNA |
| 100 |
482 |
0 |
0 |
4 |
NM_176113.1 |
CG2049-RD, transcript variant D (Pkn), mRNA |
| 100 |
482 |
0 |
0 |
0 |
NM_176110.1 |
CG2049-RB, transcript variant B (Pkn), mRNA |
| 100 |
482 |
0 |
0 |
0 |
NM_176112.1 |
CG2049-RF, transcript variant F (Pkn), mRNA |
| 0 |
0 |
4 |
2 |
14 |
NM_132358.2 |
CG9686-RA (CG9686), mRNA |
| 0 |
0 |
3 |
3 |
12 |
NM_206518.1 |
CG4720-RB, transcript variant B (Pk92B), mRNA |
| 0 |
0 |
3 |
3 |
12 |
NM_057741.3 |
CG4720-RA, transcript variant A (Pk92B), mRNA |
| 0 |
0 |
3 |
3 |
9 |
NM_080257.2 |
CG6815-RA (bor), mRNA |
| 0 |
0 |
2 |
11 |
12 |
NM_143300.1 |
CG3339-RA, transcript variant A (CG3339), mRNA |
| 0 |
0 |
2 |
11 |
12 |
NM_001043297.1 |
CG3339-RB, transcript variant B (CG3339), mRNA |
| 0 |
0 |
2 |
5 |
5 |
NM_206089.1 |
CG33473-RB (luna), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_142994.3 |
CG18528-RA (CG18528), mRNA |
| 0 |
0 |
2 |
2 |
10 |
NM_078878.2 |
CG10123-RA (Top3alpha), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_079997.2 |
CG13580-RA (Crtp), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_137836.1 |
CG12489-RA (Dnr1), mRNA |
| 0 |
0 |
1 |
9 |
10 |
NM_165675.1 |
CG11804-RA, transcript variant A (ced-6), mRNA |
| 0 |
0 |
1 |
9 |
10 |
NM_136644.2 |
CG11804-RC, transcript variant C (ced-6), mRNA |
| 0 |
0 |
1 |
9 |
10 |
NM_165676.1 |
CG11804-RB, transcript variant B (ced-6), mRNA |
| 0 |
0 |
1 |
8 |
5 |
NM_130502.2 |
CG12311-RA (Pomt2), mRNA |
| 0 |
0 |
1 |
7 |
12 |
NM_080014.2 |
CG3064-RB (futsch), mRNA |
| 0 |
0 |
1 |
7 |
10 |
NM_133085.2 |
CG6606-RA (l(1)G0003), mRNA |
| 0 |
0 |
1 |
7 |
6 |
NM_079951.2 |
CG8269-RA (Dmn), mRNA |
| 0 |
0 |
1 |
6 |
2 |
NM_142950.1 |
CG5986-RA (CG5986), mRNA |
| 0 |
0 |
1 |
4 |
6 |
NM_079617.3 |
CG7855-RA (timeout), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_080021.2 |
CG2952-RA (Dox-A3), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_141552.2 |
CG11753-RA (CG11753), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_132577.1 |
CG2556-RA (CG2556), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_167890.1 |
CG7852-RB, transcript variant B (CG7852), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_206232.1 |
CG7852-RC, transcript variant C (CG7852), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_139363.1 |
CG7852-RA, transcript variant A (CG7852), mRNA |
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