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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
2054R-1 |
| Symbol |
Cht2 |
Full Name |
Chitinase 2 |
| CG No |
CG2054 |
Old CG No |
CG2054 |
| Synonyms |
CHT2, DmCHT2, CG2054, Cht2, cht2 |
| Accession No (Link to NCBI) |
NM_057950.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
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Reference
[Please submit your publication]
|
Ohhara Y, Nakamura A, Kato Y, Yamakawa-Kobayashi K.
Chaperonin TRiC/CCT supports mitotic exit and entry into endocycle in Drosophila.
PLoS Genet (2019) 15(4) e1008121
[
PubMed ID = 31034473
]
[
RRC reference
]
Nakaoka T, Iga M, Yamada T, Koujima I, Takeshima M, Zhou X, Suzuki Y, Ogihara MH, Kataoka H.
Deep sequencing of the prothoracic gland transcriptome reveals new players in insect ecdysteroidogenesis.
PLoS One (2017) 12(3) e0172951
[
PubMed ID = 28257485
]
[
RRC reference
]
Lesch C, Jo J, Wu Y, Fish GS, Galko MJ.
A targeted UAS-RNAi screen in Drosophila larvae identifies wound closure genes regulating distinct cellular processes.
Genetics (2010) 186(3) 943-57
[
PubMed ID = 20813879
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGGTGCCTATGCCATCGAGAAC |
| Primer Seq. 3' |
aatctagaggtaccTAACGCGAGATCTGCCGCACG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 GNTGCCTaTG CCATCGAGAA CTTCGACCCC AATTTGTGCA CCCATGTGGT GTACGCCTTT
0061 GCCGGCCTGG ACATCACCCA GGCAGCCATC AAGTCGTTGG ATCCCTGGCA GGACCTAAAG
0121 GAGGAGTACG GCAAGGGTGG CTACGAGAAA ATGACGGGTC TGAAAGCGCA GCCATCCGCA
0181 TCTGAAGGTC AGCCTGGCCA TCGGCGGCTG GNAACGAGGG ATCGGCCAAC TACTCCACCC
0241 TGGTGGCCAA CAATNCTGCT GCGCGGCAGG TTCGTCAAGC AGGTGTCCAG TTTCATACGC
0301 AAGTACAACT TCGACGGGCT GGATCTCGAC TGGGAGTATC CCACGCAGCG GAAGGGCAAG
0361 CCCGCNGGAT CGCGAGNAAC TTCGTTCTGC TGACCAAGGA GCTGCGCGAG GAGTTCGACG
0421 AGCACGGCCT GCTGCTGACC TCCGCCATCG GAGCCTCCAA GAAGGTGATC GACGAGGCCT
0481 ATGACGTGCG GCAGATNCTC GCGTTA |
| in silico PCR Fragment |
0001 GGTGCCTATG CCATCGAGAA CTTCGACCCC AATTTGTGCA CCCATGTGGT GTACGCCTTT
0061 GCCGGCCTGG ACATCACCCA GGCAGCCATC AAGTCGTTGG ATCCCTGGCA GGACCTAAAG
0121 GAGGAGTACG GCAAGGGTGG CTACGAGAAA ATGACGGGTC TG-AAGCGCA GCCATCCGCA
0181 TCTGAAGGTC AGCCTGGCCA TCGGCGGCTG G-AACGAGGG ATCGGCCAAC TACTCCACCC
0241 TGGTGGCCAA CAAT-CTGCT GCGCGGCAGG TTCGTCAAGC AGGTGTCCAG TTTCATACGC
0301 AAGTACAACT TCGACGGGCT GGATCTCGAC TGGGAGTATC CCACGCAGCG GAAGGGCAAG
0361 CCCGC-GGAT CGCGAG-AAC TTCGTTCTGC TGACCAAGGA GCTGCGCGAG GAGTTCGACG
0421 AGCACGGCCT GCTGCTGACC TCCGCCATCG GAGCCTCCAA GAAGGTGATC GACGAGGCCT
0481 ATGACGTGCG GCAGAT-CTC GCGTTA |
| Assemble Data |
2054R-1.IR_full 1 GNTGCCTATGCCATCGAGAACTTCGACCCCAATTTGTGCACCCATGTGGTGTACGCCTTT 60
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2054R-1.in silico 1 GGTGCCTATGCCATCGAGAACTTCGACCCCAATTTGTGCACCCATGTGGTGTACGCCTTT 60
2054R-1.IR_full 61 GCCGGCCTGGACATCACCCAGGCAGCCATCAAGTCGTTGGATCCCTGGCAGGACCTAAAG 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2054R-1.in silico 61 GCCGGCCTGGACATCACCCAGGCAGCCATCAAGTCGTTGGATCCCTGGCAGGACCTAAAG 120
2054R-1.IR_full 121 GAGGAGTACGGCAAGGGTGGCTACGAGAAAATGACGGGTCTGAAAGCGCAGCCATCCGCA 180
|||||||||||||||||||||||||||||||||||||||||| |||||||||||||||||
2054R-1.in silico 121 GAGGAGTACGGCAAGGGTGGCTACGAGAAAATGACGGGTCTG-AAGCGCAGCCATCCGCA 180
2054R-1.IR_full 181 TCTGAAGGTCAGCCTGGCCATCGGCGGCTGGNAACGAGGGATCGGCCAACTACTCCACCC 240
||||||||||||||||||||||||||||||| ||||||||||||||||||||||||||||
2054R-1.in silico 181 TCTGAAGGTCAGCCTGGCCATCGGCGGCTGG-AACGAGGGATCGGCCAACTACTCCACCC 240
2054R-1.IR_full 241 TGGTGGCCAACAATNCTGCTGCGCGGCAGGTTCGTCAAGCAGGTGTCCAGTTTCATACGC 300
|||||||||||||| |||||||||||||||||||||||||||||||||||||||||||||
2054R-1.in silico 241 TGGTGGCCAACAAT-CTGCTGCGCGGCAGGTTCGTCAAGCAGGTGTCCAGTTTCATACGC 300
2054R-1.IR_full 301 AAGTACAACTTCGACGGGCTGGATCTCGACTGGGAGTATCCCACGCAGCGGAAGGGCAAG 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2054R-1.in silico 301 AAGTACAACTTCGACGGGCTGGATCTCGACTGGGAGTATCCCACGCAGCGGAAGGGCAAG 360
2054R-1.IR_full 361 CCCGCNGGATCGCGAGNAACTTCGTTCTGCTGACCAAGGAGCTGCGCGAGGAGTTCGACG 420
||||| |||||||||| |||||||||||||||||||||||||||||||||||||||||||
2054R-1.in silico 361 CCCGC-GGATCGCGAG-AACTTCGTTCTGCTGACCAAGGAGCTGCGCGAGGAGTTCGACG 420
2054R-1.IR_full 421 AGCACGGCCTGCTGCTGACCTCCGCCATCGGAGCCTCCAAGAAGGTGATCGACGAGGCCT 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2054R-1.in silico 421 AGCACGGCCTGCTGCTGACCTCCGCCATCGGAGCCTCCAAGAAGGTGATCGACGAGGCCT 480
2054R-1.IR_full 481 ATGACGTGCGGCAGATNCTCGCGTTA 506
|||||||||||||||| |||||||||
2054R-1.in silico 481 ATGACGTGCGGCAGAT-CTCGCGTTA 506
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_057950.2 |
CG2054-RA (Cht2), mRNA |
| 0.62 |
3 |
3 |
2 |
6 |
NM_132074.1 |
CG3585-RA (CG3585), mRNA |
| 0.62 |
3 |
3 |
2 |
2 |
NM_142924.2 |
CG10219-RA (CG10219), mRNA |
| 0.41 |
2 |
2 |
2 |
5 |
NM_168239.1 |
CG17888-RC, transcript variant C (Pdp1), mRNA |
| 0.2 |
1 |
6 |
10 |
24 |
NM_137699.3 |
CG10531-RA (CG10531), mRNA |
| 0.2 |
1 |
2 |
8 |
5 |
NM_132068.1 |
CG15899-RB (Ca-alpha1T), mRNA |
| 0.2 |
1 |
2 |
3 |
5 |
NM_140070.2 |
CG3424-RA, transcript variant A (path), mRNA |
| 0.2 |
1 |
2 |
3 |
5 |
NM_168349.1 |
CG3424-RC, transcript variant C (path), mRNA |
| 0 |
0 |
4 |
2 |
3 |
NM_143756.2 |
CG1548-RA (cathD), mRNA |
| 0 |
0 |
3 |
7 |
2 |
NM_136785.2 |
CG7712-RA (CG7712), mRNA |
| 0 |
0 |
3 |
4 |
3 |
NM_135771.2 |
CG9431-RA (kek4), mRNA |
| 0 |
0 |
3 |
3 |
3 |
NM_080223.2 |
CG3986-RA (Cht4), mRNA |
| 0 |
0 |
3 |
2 |
7 |
NM_168715.1 |
CG7930-RB, transcript variant B (TpnC73F), mRNA |
| 0 |
0 |
3 |
2 |
7 |
NM_079398.2 |
CG7930-RA, transcript variant A (TpnC73F), mRNA |
| 0 |
0 |
3 |
2 |
3 |
NM_079770.2 |
CG7748-RA (OstStt3), mRNA |
| 0 |
0 |
2 |
5 |
5 |
NM_137551.2 |
CG15117-RA, transcript variant A (CG15117), mRNA |
| 0 |
0 |
2 |
5 |
5 |
NM_166324.1 |
CG15117-RB, transcript variant B (CG15117), mRNA |
| 0 |
0 |
2 |
5 |
5 |
NM_001014535.1 |
CG15117-RC, transcript variant C (CG15117), mRNA |
| 0 |
0 |
2 |
5 |
4 |
NM_132133.1 |
CG3044-RA (CG3044), mRNA |
| 0 |
0 |
2 |
4 |
7 |
NM_137698.1 |
CG9357-RA (CG9357), mRNA |
| 0 |
0 |
2 |
4 |
6 |
NM_167908.1 |
CG7961-RB, transcript variant B (alphaCop), mRNA |
| 0 |
0 |
2 |
4 |
6 |
NM_058047.3 |
CG7961-RA, transcript variant A (alphaCop), mRNA |
| 0 |
0 |
2 |
2 |
7 |
NM_165747.2 |
CG4001-RC, transcript variant C (Pfk), mRNA |
| 0 |
0 |
2 |
2 |
7 |
NM_165746.1 |
CG4001-RB, transcript variant B (Pfk), mRNA |
| 0 |
0 |
2 |
2 |
7 |
NM_078952.2 |
CG4001-RA, transcript variant A (Pfk), mRNA |
| 0 |
0 |
2 |
2 |
6 |
NM_167080.1 |
CG32742-RA (l(1)G0148), mRNA |
| 0 |
0 |
1 |
7 |
12 |
NM_176385.1 |
CG33214-RA (CG33214), mRNA |
| 0 |
0 |
1 |
5 |
11 |
NM_132216.2 |
CG2116-RA (CG2116), mRNA |
| 0 |
0 |
1 |
5 |
8 |
NM_079447.3 |
CG8363-RE, transcript variant E (Paps), mRNA |
| 0 |
0 |
1 |
5 |
8 |
NM_168824.2 |
CG8363-RD, transcript variant D (Paps), mRNA |
|
| Wing Disc |
GFP
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Puc
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Caspase
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DAPI
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Merge
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Figure
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JNK signal
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Caspase-3 signal
| N |
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| ++ |
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| DB |
 |
| + |
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| DC |
 |
| + |
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| DM |
 |
| + |
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| VM |
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| |
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| VB |
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