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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 1958R-1 
 Symbol CG1958  Full Name CG1958 
 CG No CG1958  Old CG No CG1958 
 Synonyms CG1958 
 Accession No (Link to NCBI) NM_132158.1 
 Inserted Chr. ll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624 [ PubMed ID = 21666802 ] [ RRC reference ]

Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgCAACAAAGCTGCGATTCGCAC 
 Primer Seq. 3' 
aatctagaggtaccGTACGAGGGAGTACTTTTCAC 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 CAACAAAGCT GCGATTCGCA CTGATCACCG AGGATGTGCT GGACAAGCTG AGGCCTCGCT 
0061 CACAGTCGGA AAATGCACGA TCGAAGTACA TGAATGAAAT ATATGAGGCC ATTAGGATGG 
0121 CGGTCAACGA GGTTGTGGAC GAGAAGATGG AGCAGCTGAA TGATACAGTT AATCGAATGG 
0181 TTGAGGAGCG GGTGAGCAAA ATTCTGGACC ATCACTTGAC CAAGGGATCG TTGACGGGGC 
0241 GCAGTAGCTC AGTTGGAAGG AAAAGGGATT TGGATAATAG GGAAATCAAG GGAGCAAGGT 
0301 ATGCTGCTCC AGCCACATCA ATTAGTTCCC TAAAAGTTTC CAATCTCAAA CGTGGCCGAA 
0361 GCAAGAGGCA AAATCCCACC AAAGAGCATG TGACCTTCGC CTCCGACGAT CAGTTGACTA 
0421 AAAGGTCTAG GACCAAGGCG CAGAAAATCA CTGTAATGCC CATACATCGC GAGAGCCAGG 
0481 TGAAAAGTAC TCCCTCGTAC  
 in silico PCR Fragment 
0001 CAACAAAGCT GCGATTCGCA CTGATCACCG AGGATGTGCT GGACAAGCTG AGGCCTCGCT 
0061 CACAGTCGGA AAATGCACGA TCGAAGTACA TGAATGAAAT ATATGAGGCC ATTAGGATGG 
0121 CGGTCAACGA GGTTGTGGAC GAGAAGATGG AGCAGCTGAA TGATACAGTT AATCGAATGG 
0181 TTGAGGAGCG GGTGAGCAAA ATTCTGGACC ATCACTTGAC CAAGGGATCG TTGACGGGGC 
0241 GCAGTAGCTC AGTTGGAAGG AAAAGGGATT TGGATAATAG GGAAATCAAG GGAGCAAGGT 
0301 ATGCTGCTCC AGCCACATCA ATTAGTTCCC TAAAAGTTTC CAATCTCAAA CGTGGCCGAA 
0361 GCAAGAGGCA AAATCCCACC AAAGAGCATG TGACCTTCGC CTCCGACGAT CAGTTGACTA 
0421 AAAGGTCTAG GACCAAGGCG CAGAAAATCA CTGTAATGCC CATACATCGC GAGAGCCAGG 
0481 TGAAAAGTAC TCCCTCGTAC  
 Assemble Data 

1958R-1.IR_full       1   CAACAAAGCTGCGATTCGCACTGATCACCGAGGATGTGCTGGACAAGCTGAGGCCTCGCT 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1958R-1.in silico     1   CAACAAAGCTGCGATTCGCACTGATCACCGAGGATGTGCTGGACAAGCTGAGGCCTCGCT 60


1958R-1.IR_full       61  CACAGTCGGAAAATGCACGATCGAAGTACATGAATGAAATATATGAGGCCATTAGGATGG 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1958R-1.in silico     61  CACAGTCGGAAAATGCACGATCGAAGTACATGAATGAAATATATGAGGCCATTAGGATGG 120


1958R-1.IR_full       121 CGGTCAACGAGGTTGTGGACGAGAAGATGGAGCAGCTGAATGATACAGTTAATCGAATGG 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1958R-1.in silico     121 CGGTCAACGAGGTTGTGGACGAGAAGATGGAGCAGCTGAATGATACAGTTAATCGAATGG 180


1958R-1.IR_full       181 TTGAGGAGCGGGTGAGCAAAATTCTGGACCATCACTTGACCAAGGGATCGTTGACGGGGC 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1958R-1.in silico     181 TTGAGGAGCGGGTGAGCAAAATTCTGGACCATCACTTGACCAAGGGATCGTTGACGGGGC 240


1958R-1.IR_full       241 GCAGTAGCTCAGTTGGAAGGAAAAGGGATTTGGATAATAGGGAAATCAAGGGAGCAAGGT 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1958R-1.in silico     241 GCAGTAGCTCAGTTGGAAGGAAAAGGGATTTGGATAATAGGGAAATCAAGGGAGCAAGGT 300


1958R-1.IR_full       301 ATGCTGCTCCAGCCACATCAATTAGTTCCCTAAAAGTTTCCAATCTCAAACGTGGCCGAA 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1958R-1.in silico     301 ATGCTGCTCCAGCCACATCAATTAGTTCCCTAAAAGTTTCCAATCTCAAACGTGGCCGAA 360


1958R-1.IR_full       361 GCAAGAGGCAAAATCCCACCAAAGAGCATGTGACCTTCGCCTCCGACGATCAGTTGACTA 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1958R-1.in silico     361 GCAAGAGGCAAAATCCCACCAAAGAGCATGTGACCTTCGCCTCCGACGATCAGTTGACTA 420


1958R-1.IR_full       421 AAAGGTCTAGGACCAAGGCGCAGAAAATCACTGTAATGCCCATACATCGCGAGAGCCAGG 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1958R-1.in silico     421 AAAGGTCTAGGACCAAGGCGCAGAAAATCACTGTAATGCCCATACATCGCGAGAGCCAGG 480


1958R-1.IR_full       481 TGAAAAGTACTCCCTCGTAC 500
                          ||||||||||||||||||||
1958R-1.in silico     481 TGAAAAGTACTCCCTCGTAC 500



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_132158.1  CG1958-RA (CG1958), mRNA 
0.62   3  2  3  5  NM_001043297.1  CG3339-RB, transcript variant B (CG3339), mRNA 
0.62   3  2  3  5  NM_143300.1  CG3339-RA, transcript variant A (CG3339), mRNA 
0.41   2  3  2  4  NM_136121.1  CG9987-RA (CG9987), mRNA 
0   0  3  2  6  NM_167021.1  CG2861-RB, transcript variant B (CG2861), mRNA 
0   0  2  4  2  NM_168003.2  CG32270-RA, transcript variant A (CG32270), mRNA 
0   0  2  4  2  NM_139531.3  CG32269-RA, transcript variant A (CG32269), mRNA 
0   0  1  4  8  NM_167028.1  CG6775-RB, transcript variant B (rg), mRNA 
0   0  1  4  8  NM_080023.1  CG6775-RA, transcript variant A (rg), mRNA 
0   0  1  4  8  NM_001042796.1  CG6775-RC, transcript variant C (rg), mRNA 
0   0  1  4  2  NM_130709.1  CG14271-RB (Gas8), mRNA 
0   0  1  3  4  NM_165018.1  CG6167-RB, transcript variant B (PICK1), mRNA 
0   0  1  3  4  NM_135738.2  CG6167-RA, transcript variant A (PICK1), mRNA 
0   0  1  3  3  NM_165842.1  CG10897-RC, transcript variant C (tou), mRNA 
0   0  1  3  3  NM_078977.2  CG10897-RA, transcript variant A (tou), mRNA 
0   0  1  3  2  NM_145761.1  CG16778-RC, transcript variant C (Tkr), mRNA 
0   0  1  3  2  NM_079866.2  CG1744-RA (chp), mRNA 
0   0  1  3  2  NM_079141.2  CG16778-RB, transcript variant B (Tkr), mRNA 
0   0  1  3  2  NM_001014554.1  CG16778-RD, transcript variant D (Tkr), mRNA 
0   0  1  3  2  NM_138150.2  CG16778-RA, transcript variant A (Tkr), mRNA 
0   0  1  2  4  NM_080314.2  CG8590-RA (Klp3A), mRNA 
0   0  0  8  9  NM_205875.1  CG32019-RE, transcript variant E (bt), mRNA 
0   0  0  8  9  NM_205877.1  CG32019-RD, transcript variant D (bt), mRNA 
0   0  0  8  9  NM_166790.2  CG32019-RA, transcript variant A (bt), mRNA 
0   0  0  6  5  NM_080018.1  CG6890-RA (Tollo), mRNA 
0   0  0  5  5  NM_137543.2  CG15106-RA (Jheh3), mRNA 
0   0  0  5  4  NM_143640.1  CG11574-RA (CG11574), mRNA 
0   0  0  5  2  NM_140003.1  CG13312-RA (CG13312), mRNA 
0   0  0  4  9  NM_143807.2  CG3558-RA, transcript variant A (CG3558), mRNA 
0   0  0  4  9  NM_164519.1  CG3558-RB, transcript variant B (CG3558), mRNA 
 Wing Disc
GFP
 Puc
 Caspase
DAPI
 Merge
 Figure
JNK signal
N
 
DB
 
DC
+- 
DM
 
VM
 
VB
 
Caspase-3 signal
N
+ 
DB
 
DC
++ 
DM
 
VM
 
VB
 
Dcompartment   Penetrance
     

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